(Circular) (Six-base) MAPSORT of: pF1KB9548.seq Check: 6473 from: 1 to: 3494 pF1KB9548 3494 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AccI GT'mk_AC Cuts at: 532 532 Size: 3494 Acc65I G'GTAC_C Cuts at: 510 510 Size: 3494 AclI AA'CG_TT Cuts at: 3334 3334 Size: 3494 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1319 1751 2104 2766 1319 Size: 432 353 662 2047 Fragments arranged by size: 2047 662 432 353 AfeI AGC'GCT Cuts at: 317 317 Size: 3494 AflIII A'CryG_T Cuts at: 846 2646 2927 846 Size: 1800 281 1413 Fragments arranged by size: 1800 1413 281 AlwNI CAG_nnn'CTG Cuts at: 799 2237 799 Size: 1438 2056 ApaLI G'TGCA_C Cuts at: 2332 2332 Size: 3494 ApoI r'AATT_y Cuts at: 498 2847 3103 498 Size: 2349 256 889 Fragments arranged by size: 2349 889 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3494 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3494 AvaI C'yCGr_G Cuts at: 311 514 311 Size: 203 3291 BamHI G'GATC_C Cuts at: 519 519 Size: 3494 BanI G'GyrC_C Cuts at: 307 510 924 1172 1207 307 Size: 203 414 248 35 2594 Fragments arranged by size: 2594 414 248 203 35 BanII G_rGCy'C Cuts at: 297 476 508 1538 297 Size: 179 32 1030 2253 Fragments arranged by size: 2253 1030 179 32 BbeI G_GCGC'C Cuts at: 928 1176 928 Size: 248 3246 BbsI GAAGACnn'nnnn_ Cuts at: 461 461 Size: 3494 BciVI GTATCCnnnnn_n' Cuts at: 1392 2448 3494 1392 Size: 1056 1046 1392 Fragments arranged by size: 1392 1056 1046 BclI T'GATC_A Cuts at: 1014 1014 Size: 3494 BglII A'GATC_T Cuts at: 1009 3046 1009 Size: 2037 1457 BlpI GC'TnA_GC Cuts at: 606 606 Size: 3494 Bme1580I G_kGCm'C Cuts at: 310 339 1119 1212 2336 310 Size: 29 780 93 1124 1468 Fragments arranged by size: 1468 1124 780 93 29 BmrI ACTGGGnnnn_n' Cuts at: 468 706 787 1122 3266 468 Size: 238 81 335 2144 696 Fragments arranged by size: 2144 696 335 238 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1861 1861 Size: 3494 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 304 376 304 Size: 72 3422 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 382 670 1549 2263 2561 382 Size: 288 879 714 298 1315 Fragments arranged by size: 1315 879 714 298 288 BsaI GGTCTCn'nnnn_ Cuts at: 3198 3198 Size: 3494 BsaAI yAC'GTr Cuts at: 847 847 Size: 3494 BsaBI GATnn'nnATC Cuts at: 6 415 1008 3045 6 Size: 409 593 2037 455 Fragments arranged by size: 2037 593 455 409 BsaHI Gr'CG_yC Cuts at: 925 1173 1875 3371 925 Size: 248 702 1496 1048 Fragments arranged by size: 1496 1048 702 248 BsaWI w'CCGG_w Cuts at: 683 1204 1882 2293 2440 2714 683 Size: 521 678 411 147 274 1463 Fragments arranged by size: 1463 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 172 172 Size: 3494 BseYI C'CCAG_C Cuts at: 303 919 1677 2342 2750 303 Size: 616 758 665 408 1047 Fragments arranged by size: 1047 758 665 616 408 BsiEI CG_ry'CG Cuts at: 104 241 1036 1082 2312 104 Size: 137 795 46 1230 1286 Fragments arranged by size: 1286 1230 795 137 46 BsiHKAI G_wGCw'C Cuts at: 476 508 1286 1476 2336 476 Size: 32 778 190 860 1634 Fragments arranged by size: 1634 860 778 190 32 BsmI GAATG_Cn' Cuts at: 192 192 Size: 3494 Bsp1286I G_dGCh'C Cuts at: 297 310 339 476 508 1119 1212 1286 1476 Size: 13 29 137 32 611 93 74 190 Cuts at: 1476 1538 2336 297 Size: 62 798 1455 Fragments arranged by size: 1455 798 611 190 137 93 74 62 32 29 13 BspHI T'CATG_A Cuts at: 1926 1926 Size: 3494 BspMI ACCTGCnnnn'nnnn_ Cuts at: 443 544 758 1060 1441 443 Size: 101 214 302 381 2496 Fragments arranged by size: 2496 381 302 214 101 BsrBI CCG'CTC Cuts at: 50 1786 1840 3324 3489 50 Size: 1736 54 1484 165 55 Fragments arranged by size: 1736 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 380 1406 380 Size: 1026 2468 BsrFI r'CCGG_y Cuts at: 1492 1673 1492 Size: 181 3313 BssSI C'ACGA_G Cuts at: 1765 2473 1765 Size: 708 2786 BstAPI GCAn_nnn'nTGC Cuts at: 871 871 Size: 3494 BstBI TT'CG_AA Cuts at: 1855 1855 Size: 3494 BstYI r'GATC_y Cuts at: 1 218 369 410 519 986 1009 1344 1590 Size: 217 151 41 109 467 23 335 246 Cuts at: 1590 1896 1908 1994 3046 1 Size: 306 12 86 1052 449 Fragments arranged by size: 1052 467 449 335 306 246 217 151 109 86 41 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 343 1444 1625 1788 343 Size: 1101 181 163 2049 Fragments arranged by size: 2049 1101 181 163 BtsI GCAGTG_nn' Cuts at: 737 737 Size: 3494 DraI TTT'AAA Cuts at: 493 493 Size: 3494 DrdI GACnn_nn'nnGTC Cuts at: 1200 2544 1200 Size: 1344 2150 EaeI y'GGCC_r Cuts at: 1079 1253 1644 1671 3016 1079 Size: 174 391 27 1345 1557 Fragments arranged by size: 1557 1345 391 174 27 EagI C'GGCC_G Cuts at: 1079 1079 Size: 3494 EarI CTCTTCn'nnn_ Cuts at: 188 1517 1727 188 Size: 1329 210 1955 Fragments arranged by size: 1955 1329 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1702 2444 2590 3304 1702 Size: 742 146 714 1892 Fragments arranged by size: 1892 742 714 146 EcoICRI GAG'CTC Cuts at: 474 506 474 Size: 32 3462 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 304 376 1319 1751 2104 2766 304 Size: 72 943 432 353 662 1032 Fragments arranged by size: 1032 943 662 432 353 72 EcoO109I rG'GnC_Cy Cuts at: 633 633 Size: 3494 EcoRI G'AATT_C Cuts at: 498 498 Size: 3494 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1513 1513 Size: 3494 FspI TGC'GCA Cuts at: 1275 1275 Size: 3494 HaeII r_GCGC'y Cuts at: 319 928 1176 2406 3177 319 Size: 609 248 1230 771 636 Fragments arranged by size: 1230 771 636 609 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 357 389 1336 1366 1368 1398 357 Size: 32 947 30 2 30 2453 Fragments arranged by size: 2453 947 32 30 30 2 HincII GTy'rAC Cuts at: 533 533 Size: 3494 KasI G'GCGC_C Cuts at: 924 1172 924 Size: 248 3246 KpnI G_GTAC'C Cuts at: 514 514 Size: 3494 MluI A'CGCG_T Cuts at: 2927 2927 Size: 3494 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2253 2437 2253 Size: 184 3310 MscI TGG'CCA Cuts at: 1255 1255 Size: 3494 MslI CAynn'nnrTG Cuts at: 385 1483 1610 385 Size: 1098 127 2269 Fragments arranged by size: 2269 1098 127 MspA1I CmG'CkG Cuts at: 605 919 1279 2063 2308 605 Size: 314 360 784 245 1791 Fragments arranged by size: 1791 784 360 314 245 NaeI GCC'GGC Cuts at: 1675 1675 Size: 3494 NarI GG'CG_CC Cuts at: 925 1173 925 Size: 248 3246 NgoMIV G'CCGG_C Cuts at: 1673 1673 Size: 3494 NspI r_CATG'y Cuts at: 547 1047 2650 547 Size: 500 1603 1391 Fragments arranged by size: 1603 1391 500 PciI A'CATG_T Cuts at: 2646 2646 Size: 3494 PmeI GTTT'AAAC Cuts at: 493 493 Size: 3494 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1940 1972 1940 Size: 32 3462 PstI C_TGCA'G Cuts at: 541 1226 541 Size: 685 2809 PvuI CG_AT'CG Cuts at: 104 104 Size: 3494 PvuII CAG'CTG Cuts at: 919 1279 919 Size: 360 3134 RsrII CG'GwC_CG Cuts at: 1689 1689 Size: 3494 SacI G_AGCT'C Cuts at: 476 508 476 Size: 32 3462 SalI G'TCGA_C Cuts at: 531 531 Size: 3494 SapI GCTCTTCn'nnn_ Cuts at: 1517 1727 1517 Size: 210 3284 SbfI CC_TGCA'GG Cuts at: 541 541 Size: 3494 ScaI AGT'ACT Cuts at: 3011 3011 Size: 3494 SfcI C'TryA_G Cuts at: 33 537 1222 2190 2381 33 Size: 504 685 968 191 1146 Fragments arranged by size: 1146 968 685 504 191 SfoI GGC'GCC Cuts at: 926 1174 926 Size: 248 3246 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3494 SmaI CCC'GGG Cuts at: 516 516 Size: 3494 SmlI C'TyrA_G Cuts at: 397 649 1564 2278 2540 397 Size: 252 915 714 262 1351 Fragments arranged by size: 1351 915 714 262 252 SpeI A'CTAG_T Cuts at: 67 67 Size: 3494 SphI G_CATG'C Cuts at: 547 1047 547 Size: 500 2994 StyI C'CwwG_G Cuts at: 628 982 628 Size: 354 3140 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1077 1210 1669 1878 1077 Size: 133 459 209 2693 Fragments arranged by size: 2693 459 209 133 TatI w'GTAC_w Cuts at: 3009 3009 Size: 3494 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3011 3043 3011 Size: 32 3462 Tth111I GACn'n_nGTC Cuts at: 1291 1291 Size: 3494 XbaI T'CTAG_A Cuts at: 525 525 Size: 3494 XcmI CCAnnnn_n'nnnnTGG Cuts at: 989 989 Size: 3494 XmaI C'CCGG_G Cuts at: 514 514 Size: 3494 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AgeI AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI