Restriction map for
pF1KB9548
(Circular) (Six-base) MAPSORT of: pF1KB9548.seq Check: 6473 from: 1 to: 3494
pF1KB9548 3494 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 532 532
Size: 3494
Acc65I G'GTAC_C
Cuts at: 510 510
Size: 3494
AclI AA'CG_TT
Cuts at: 3334 3334
Size: 3494
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1319 1751 2104 2766 1319
Size: 432 353 662 2047
Fragments arranged by size:
2047 662 432 353
AfeI AGC'GCT
Cuts at: 317 317
Size: 3494
AflIII A'CryG_T
Cuts at: 846 2646 2927 846
Size: 1800 281 1413
Fragments arranged by size:
1800 1413 281
AlwNI CAG_nnn'CTG
Cuts at: 799 2237 799
Size: 1438 2056
ApaLI G'TGCA_C
Cuts at: 2332 2332
Size: 3494
ApoI r'AATT_y
Cuts at: 498 2847 3103 498
Size: 2349 256 889
Fragments arranged by size:
2349 889 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3494
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3494
AvaI C'yCGr_G
Cuts at: 311 514 311
Size: 203 3291
BamHI G'GATC_C
Cuts at: 519 519
Size: 3494
BanI G'GyrC_C
Cuts at: 307 510 924 1172 1207 307
Size: 203 414 248 35 2594
Fragments arranged by size:
2594 414 248 203 35
BanII G_rGCy'C
Cuts at: 297 476 508 1538 297
Size: 179 32 1030 2253
Fragments arranged by size:
2253 1030 179 32
BbeI G_GCGC'C
Cuts at: 928 1176 928
Size: 248 3246
BbsI GAAGACnn'nnnn_
Cuts at: 461 461
Size: 3494
BciVI GTATCCnnnnn_n'
Cuts at: 1392 2448 3494 1392
Size: 1056 1046 1392
Fragments arranged by size:
1392 1056 1046
BclI T'GATC_A
Cuts at: 1014 1014
Size: 3494
BglII A'GATC_T
Cuts at: 1009 3046 1009
Size: 2037 1457
BlpI GC'TnA_GC
Cuts at: 606 606
Size: 3494
Bme1580I G_kGCm'C
Cuts at: 310 339 1119 1212 2336 310
Size: 29 780 93 1124 1468
Fragments arranged by size:
1468 1124 780 93 29
BmrI ACTGGGnnnn_n'
Cuts at: 468 706 787 1122 3266 468
Size: 238 81 335 2144 696
Fragments arranged by size:
2144 696 335 238 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 376 304
Size: 72 3422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 670 1549 2263 2561 382
Size: 288 879 714 298 1315
Fragments arranged by size:
1315 879 714 298 288
BsaI GGTCTCn'nnnn_
Cuts at: 3198 3198
Size: 3494
BsaAI yAC'GTr
Cuts at: 847 847
Size: 3494
BsaBI GATnn'nnATC
Cuts at: 6 415 1008 3045 6
Size: 409 593 2037 455
Fragments arranged by size:
2037 593 455 409
BsaHI Gr'CG_yC
Cuts at: 925 1173 1875 3371 925
Size: 248 702 1496 1048
Fragments arranged by size:
1496 1048 702 248
BsaWI w'CCGG_w
Cuts at: 683 1204 1882 2293 2440 2714 683
Size: 521 678 411 147 274 1463
Fragments arranged by size:
1463 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 172
Size: 3494
BseYI C'CCAG_C
Cuts at: 303 919 1677 2342 2750 303
Size: 616 758 665 408 1047
Fragments arranged by size:
1047 758 665 616 408
BsiEI CG_ry'CG
Cuts at: 104 241 1036 1082 2312 104
Size: 137 795 46 1230 1286
Fragments arranged by size:
1286 1230 795 137 46
BsiHKAI G_wGCw'C
Cuts at: 476 508 1286 1476 2336 476
Size: 32 778 190 860 1634
Fragments arranged by size:
1634 860 778 190 32
BsmI GAATG_Cn'
Cuts at: 192 192
Size: 3494
Bsp1286I G_dGCh'C
Cuts at: 297 310 339 476 508 1119 1212 1286 1476
Size: 13 29 137 32 611 93 74 190
Cuts at: 1476 1538 2336 297
Size: 62 798 1455
Fragments arranged by size:
1455 798 611 190 137 93 74 62
32 29 13
BspHI T'CATG_A
Cuts at: 1926 1926
Size: 3494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 443 544 758 1060 1441 443
Size: 101 214 302 381 2496
Fragments arranged by size:
2496 381 302 214 101
BsrBI CCG'CTC
Cuts at: 50 1786 1840 3324 3489 50
Size: 1736 54 1484 165 55
Fragments arranged by size:
1736 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 380 1406 380
Size: 1026 2468
BsrFI r'CCGG_y
Cuts at: 1492 1673 1492
Size: 181 3313
BssSI C'ACGA_G
Cuts at: 1765 2473 1765
Size: 708 2786
BstAPI GCAn_nnn'nTGC
Cuts at: 871 871
Size: 3494
BstBI TT'CG_AA
Cuts at: 1855 1855
Size: 3494
BstYI r'GATC_y
Cuts at: 1 218 369 410 519 986 1009 1344 1590
Size: 217 151 41 109 467 23 335 246
Cuts at: 1590 1896 1908 1994 3046 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 467 449 335 306 246 217 151
109 86 41 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 343 1444 1625 1788 343
Size: 1101 181 163 2049
Fragments arranged by size:
2049 1101 181 163
BtsI GCAGTG_nn'
Cuts at: 737 737
Size: 3494
DraI TTT'AAA
Cuts at: 493 493
Size: 3494
DrdI GACnn_nn'nnGTC
Cuts at: 1200 2544 1200
Size: 1344 2150
EaeI y'GGCC_r
Cuts at: 1079 1253 1644 1671 3016 1079
Size: 174 391 27 1345 1557
Fragments arranged by size:
1557 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1079 1079
Size: 3494
EarI CTCTTCn'nnn_
Cuts at: 188 1517 1727 188
Size: 1329 210 1955
Fragments arranged by size:
1955 1329 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1702 2444 2590 3304 1702
Size: 742 146 714 1892
Fragments arranged by size:
1892 742 714 146
EcoICRI GAG'CTC
Cuts at: 474 506 474
Size: 32 3462
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 376 1319 1751 2104 2766 304
Size: 72 943 432 353 662 1032
Fragments arranged by size:
1032 943 662 432 353 72
EcoO109I rG'GnC_Cy
Cuts at: 633 633
Size: 3494
EcoRI G'AATT_C
Cuts at: 498 498
Size: 3494
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1513 1513
Size: 3494
FspI TGC'GCA
Cuts at: 1275 1275
Size: 3494
HaeII r_GCGC'y
Cuts at: 319 928 1176 2406 3177 319
Size: 609 248 1230 771 636
Fragments arranged by size:
1230 771 636 609 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 357 389 1336 1366 1368 1398 357
Size: 32 947 30 2 30 2453
Fragments arranged by size:
2453 947 32 30 30 2
HincII GTy'rAC
Cuts at: 533 533
Size: 3494
KasI G'GCGC_C
Cuts at: 924 1172 924
Size: 248 3246
KpnI G_GTAC'C
Cuts at: 514 514
Size: 3494
MluI A'CGCG_T
Cuts at: 2927 2927
Size: 3494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2253 2437 2253
Size: 184 3310
MscI TGG'CCA
Cuts at: 1255 1255
Size: 3494
MslI CAynn'nnrTG
Cuts at: 385 1483 1610 385
Size: 1098 127 2269
Fragments arranged by size:
2269 1098 127
MspA1I CmG'CkG
Cuts at: 605 919 1279 2063 2308 605
Size: 314 360 784 245 1791
Fragments arranged by size:
1791 784 360 314 245
NaeI GCC'GGC
Cuts at: 1675 1675
Size: 3494
NarI GG'CG_CC
Cuts at: 925 1173 925
Size: 248 3246
NgoMIV G'CCGG_C
Cuts at: 1673 1673
Size: 3494
NspI r_CATG'y
Cuts at: 547 1047 2650 547
Size: 500 1603 1391
Fragments arranged by size:
1603 1391 500
PciI A'CATG_T
Cuts at: 2646 2646
Size: 3494
PmeI GTTT'AAAC
Cuts at: 493 493
Size: 3494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1940 1972 1940
Size: 32 3462
PstI C_TGCA'G
Cuts at: 541 1226 541
Size: 685 2809
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3494
PvuII CAG'CTG
Cuts at: 919 1279 919
Size: 360 3134
RsrII CG'GwC_CG
Cuts at: 1689 1689
Size: 3494
SacI G_AGCT'C
Cuts at: 476 508 476
Size: 32 3462
SalI G'TCGA_C
Cuts at: 531 531
Size: 3494
SapI GCTCTTCn'nnn_
Cuts at: 1517 1727 1517
Size: 210 3284
SbfI CC_TGCA'GG
Cuts at: 541 541
Size: 3494
ScaI AGT'ACT
Cuts at: 3011 3011
Size: 3494
SfcI C'TryA_G
Cuts at: 33 537 1222 2190 2381 33
Size: 504 685 968 191 1146
Fragments arranged by size:
1146 968 685 504 191
SfoI GGC'GCC
Cuts at: 926 1174 926
Size: 248 3246
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3494
SmaI CCC'GGG
Cuts at: 516 516
Size: 3494
SmlI C'TyrA_G
Cuts at: 397 649 1564 2278 2540 397
Size: 252 915 714 262 1351
Fragments arranged by size:
1351 915 714 262 252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3494
SphI G_CATG'C
Cuts at: 547 1047 547
Size: 500 2994
StyI C'CwwG_G
Cuts at: 628 982 628
Size: 354 3140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1077 1210 1669 1878 1077
Size: 133 459 209 2693
Fragments arranged by size:
2693 459 209 133
TatI w'GTAC_w
Cuts at: 3009 3009
Size: 3494
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3462
Tth111I GACn'n_nGTC
Cuts at: 1291 1291
Size: 3494
XbaI T'CTAG_A
Cuts at: 525 525
Size: 3494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 989 989
Size: 3494
XmaI C'CCGG_G
Cuts at: 514 514
Size: 3494
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BspEI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI