Restriction map for
pF1KB9549
(Circular) (Six-base) MAPSORT of: pF1KB9549.seq Check: 7387 from: 1 to: 4517
pF1KB9549 4517 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1349 1349
Size: 4517
AccI GT'mk_AC
Cuts at: 1555 1555
Size: 4517
Acc65I G'GTAC_C
Cuts at: 1533 1533
Size: 4517
AclI AA'CG_TT
Cuts at: 4357 4357
Size: 4517
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 2342 2774 3127 3789 200
Size: 2142 432 353 662 928
Fragments arranged by size:
2142 928 662 432 353
AflIII A'CryG_T
Cuts at: 458 1323 1505 1869 3669 3950 458
Size: 865 182 364 1800 281 1025
Fragments arranged by size:
1800 1025 865 364 281 182
AleI CACnn'nnGTG
Cuts at: 461 946 461
Size: 485 4032
AlwNI CAG_nnn'CTG
Cuts at: 1822 3260 1822
Size: 1438 3079
ApaLI G'TGCA_C
Cuts at: 939 3355 939
Size: 2416 2101
ApoI r'AATT_y
Cuts at: 668 973 1132 1521 3870 4126 668
Size: 305 159 389 2349 256 1059
Fragments arranged by size:
2349 1059 389 305 256 159
AseI AT'TA_AT
Cuts at: 20 20
Size: 4517
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
AvaI C'yCGr_G
Cuts at: 148 782 1537 148
Size: 634 755 3128
Fragments arranged by size:
3128 755 634
BamHI G'GATC_C
Cuts at: 1542 1542
Size: 4517
BanI G'GyrC_C
Cuts at: 1533 1947 2195 2230 1533
Size: 414 248 35 3820
Fragments arranged by size:
3820 414 248 35
BanII G_rGCy'C
Cuts at: 172 340 968 1531 2561 172
Size: 168 628 563 1030 2128
Fragments arranged by size:
2128 1030 628 563 168
BbeI G_GCGC'C
Cuts at: 1951 2199 1951
Size: 248 4269
BbsI GAAGACnn'nnnn_
Cuts at: 868 1064 1459 868
Size: 196 395 3926
Fragments arranged by size:
3926 395 196
BciVI GTATCCnnnnn_n'
Cuts at: 2415 3471 4517 2415
Size: 1056 1046 2415
Fragments arranged by size:
2415 1056 1046
BclI T'GATC_A
Cuts at: 2037 2037
Size: 4517
BglII A'GATC_T
Cuts at: 640 711 1493 2032 4069 640
Size: 71 782 539 2037 1088
Fragments arranged by size:
2037 1088 782 539 71
BlpI GC'TnA_GC
Cuts at: 1629 1629
Size: 4517
Bme1580I G_kGCm'C
Cuts at: 943 2142 2235 3359 943
Size: 1199 93 1124 2101
Fragments arranged by size:
2101 1199 1124 93
BmgBI CAC'GTC
Cuts at: 944 944
Size: 4517
BmrI ACTGGGnnnn_n'
Cuts at: 383 1729 1810 2145 4289 383
Size: 1346 81 335 2144 611
Fragments arranged by size:
2144 1346 611 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2884 2884
Size: 4517
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 1693 2572 3286 3584 487
Size: 1206 879 714 298 1420
Fragments arranged by size:
1420 1206 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 946 4221 946
Size: 3275 1242
BsaAI yAC'GTr
Cuts at: 1870 1870
Size: 4517
BsaBI GATnn'nnATC
Cuts at: 6 2031 4068 6
Size: 2025 2037 455
Fragments arranged by size:
2037 2025 455
BsaHI Gr'CG_yC
Cuts at: 1948 2196 2898 4394 1948
Size: 248 702 1496 2071
Fragments arranged by size:
2071 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1706 2227 2905 3316 3463 3737 1706
Size: 521 678 411 147 274 2486
Fragments arranged by size:
2486 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 832 862 832
Size: 30 4487
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 333 333
Size: 4517
BseYI C'CCAG_C
Cuts at: 1942 2700 3365 3773 1942
Size: 758 665 408 2686
Fragments arranged by size:
2686 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1459 1459
Size: 4517
BsiEI CG_ry'CG
Cuts at: 104 528 2059 2105 3335 104
Size: 424 1531 46 1230 1286
Fragments arranged by size:
1531 1286 1230 424 46
BsiHKAI G_wGCw'C
Cuts at: 943 968 1331 1531 2309 2499 3359 943
Size: 25 363 200 778 190 860 2101
Fragments arranged by size:
2101 860 778 363 200 190 25
BsmI GAATG_Cn'
Cuts at: 638 638
Size: 4517
Bsp1286I G_dGCh'C
Cuts at: 172 340 943 968 1331 1531 2142 2235 2309
Size: 168 603 25 363 200 611 93 74
Cuts at: 2309 2499 2561 3359 172
Size: 190 62 798 1330
Fragments arranged by size:
1330 798 611 603 363 200 190 168
93 74 62 25
BspHI T'CATG_A
Cuts at: 2949 2949
Size: 4517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1349 1567 1781 2083 2464 1349
Size: 218 214 302 381 3402
Fragments arranged by size:
3402 381 302 218 214
BsrBI CCG'CTC
Cuts at: 50 2809 2863 4347 4512 50
Size: 2759 54 1484 165 55
Fragments arranged by size:
2759 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1027 2429 1027
Size: 1402 3115
BsrFI r'CCGG_y
Cuts at: 154 2515 2696 154
Size: 2361 181 1975
Fragments arranged by size:
2361 1975 181
BsrGI T'GTAC_A
Cuts at: 1320 1320
Size: 4517
BssSI C'ACGA_G
Cuts at: 1127 2788 3496 1127
Size: 1661 708 2148
Fragments arranged by size:
2148 1661 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1437 1894 1437
Size: 457 4060
BstBI TT'CG_AA
Cuts at: 2878 2878
Size: 4517
BstXI CCAn_nnnn'nTGG
Cuts at: 882 882
Size: 4517
BstYI r'GATC_y
Cuts at: 1 539 640 711 1493 1542 2009 2032 2367
Size: 538 101 71 782 49 467 23 335
Cuts at: 2367 2613 2919 2931 3017 4069 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 782 538 467 449 335 306 246
101 86 71 49 23 12
Bsu36I CC'TnA_GG
Cuts at: 435 1147 435
Size: 712 3805
BtgI C'CryG_G
Cuts at: 107 107
Size: 4517
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2467 2648 2811 2467
Size: 181 163 4173
Fragments arranged by size:
4173 181 163
BtsI GCAGTG_nn'
Cuts at: 927 1760 927
Size: 833 3684
DraI TTT'AAA
Cuts at: 1516 1516
Size: 4517
DrdI GACnn_nn'nnGTC
Cuts at: 2223 3567 2223
Size: 1344 3173
EaeI y'GGCC_r
Cuts at: 725 872 884 2102 2276 2667 2694 4039 725
Size: 147 12 1218 174 391 27 1345 1203
Fragments arranged by size:
1345 1218 1203 391 174 147 27 12
EagI C'GGCC_G
Cuts at: 2102 2102
Size: 4517
EarI CTCTTCn'nnn_
Cuts at: 176 1378 2540 2750 176
Size: 1202 1162 210 1943
Fragments arranged by size:
1943 1202 1162 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 925 2725 3467 3613 4327 925
Size: 1800 742 146 714 1115
Fragments arranged by size:
1800 1115 742 714 146
EcoICRI GAG'CTC
Cuts at: 966 1529 966
Size: 563 3954
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 2342 2774 3127 3789 200
Size: 2142 432 353 662 928
Fragments arranged by size:
2142 928 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1656 1656
Size: 4517
EcoRI G'AATT_C
Cuts at: 973 1132 1521 973
Size: 159 389 3969
Fragments arranged by size:
3969 389 159
EcoRV GAT'ATC
Cuts at: 1167 1230 1365 1167
Size: 63 135 4319
Fragments arranged by size:
4319 135 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 498 2536 498
Size: 2038 2479
FspI TGC'GCA
Cuts at: 1430 2298 1430
Size: 868 3649
HaeII r_GCGC'y
Cuts at: 1951 2199 3429 4200 1951
Size: 248 1230 771 2268
Fragments arranged by size:
2268 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 703 735 2359 2389 2391 2421 418
Size: 32 253 32 1624 30 2 30 2514
Fragments arranged by size:
2514 1624 253 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1556 1556
Size: 4517
HindIII A'AGCT_T
Cuts at: 249 249
Size: 4517
KasI G'GCGC_C
Cuts at: 1947 2195 1947
Size: 248 4269
KpnI G_GTAC'C
Cuts at: 1537 1537
Size: 4517
MluI A'CGCG_T
Cuts at: 458 3950 458
Size: 3492 1025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1442 3276 3460 1442
Size: 1834 184 2499
Fragments arranged by size:
2499 1834 184
MscI TGG'CCA
Cuts at: 727 874 886 2278 727
Size: 147 12 1392 2966
Fragments arranged by size:
2966 1392 147 12
MslI CAynn'nnrTG
Cuts at: 461 946 2506 2633 461
Size: 485 1560 127 2345
Fragments arranged by size:
2345 1560 485 127
MspA1I CmG'CkG
Cuts at: 533 1413 1434 1628 1942 2302 3086 3331 533
Size: 880 21 194 314 360 784 245 1719
Fragments arranged by size:
1719 880 784 360 314 245 194 21
NaeI GCC'GGC
Cuts at: 156 2698 156
Size: 2542 1975
NarI GG'CG_CC
Cuts at: 1948 2196 1948
Size: 248 4269
NcoI C'CATG_G
Cuts at: 107 107
Size: 4517
NgoMIV G'CCGG_C
Cuts at: 154 2696 154
Size: 2542 1975
NspI r_CATG'y
Cuts at: 1327 1429 1509 1570 2070 3673 1327
Size: 102 80 61 500 1603 2171
Fragments arranged by size:
2171 1603 500 102 80 61
PciI A'CATG_T
Cuts at: 1323 1505 3669 1323
Size: 182 2164 2171
Fragments arranged by size:
2171 2164 182
PmeI GTTT'AAAC
Cuts at: 1516 1516
Size: 4517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 4485
PsiI TTA'TAA
Cuts at: 1123 1123
Size: 4517
PspXI vC'TCGA_Gb
Cuts at: 148 148
Size: 4517
PstI C_TGCA'G
Cuts at: 711 1346 1490 1564 2249 711
Size: 635 144 74 685 2979
Fragments arranged by size:
2979 685 635 144 74
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4517
PvuII CAG'CTG
Cuts at: 533 1413 1434 1942 2302 533
Size: 880 21 508 360 2748
Fragments arranged by size:
2748 880 508 360 21
RsrII CG'GwC_CG
Cuts at: 2712 2712
Size: 4517
SacI G_AGCT'C
Cuts at: 968 1531 968
Size: 563 3954
SalI G'TCGA_C
Cuts at: 1554 1554
Size: 4517
SapI GCTCTTCn'nnn_
Cuts at: 2540 2750 2540
Size: 210 4307
SbfI CC_TGCA'GG
Cuts at: 1564 1564
Size: 4517
ScaI AGT'ACT
Cuts at: 4034 4034
Size: 4517
SfcI C'TryA_G
Cuts at: 33 707 1342 1486 1497 1560 2245 3213 3404
Size: 674 635 144 11 63 685 968 191
Cuts at: 3404 33
Size: 1146
Fragments arranged by size:
1146 968 685 674 635 191 144 63
11
SfoI GGC'GCC
Cuts at: 1949 2197 1949
Size: 248 4269
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
SmaI CCC'GGG
Cuts at: 784 1539 784
Size: 755 3762
SmlI C'TyrA_G
Cuts at: 148 502 1672 2587 3301 3563 148
Size: 354 1170 915 714 262 1102
Fragments arranged by size:
1170 1102 915 714 354 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4517
SphI G_CATG'C
Cuts at: 1429 1570 2070 1429
Size: 141 500 3876
Fragments arranged by size:
3876 500 141
StuI AGG'CCT
Cuts at: 433 745 433
Size: 312 4205
StyI C'CwwG_G
Cuts at: 107 980 1651 2005 107
Size: 873 671 354 2619
Fragments arranged by size:
2619 873 671 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 294 2100 2233 2692 2901 294
Size: 1806 133 459 209 1910
Fragments arranged by size:
1910 1806 459 209 133
TatI w'GTAC_w
Cuts at: 1320 4032 1320
Size: 2712 1805
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 832 864 4034 4066 832
Size: 32 3170 32 1283
Fragments arranged by size:
3170 1283 32 32
Tth111I GACn'n_nGTC
Cuts at: 1458 2314 1458
Size: 856 3661
XbaI T'CTAG_A
Cuts at: 162 1548 162
Size: 1386 3131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2012 2012
Size: 4517
XhoI C'TCGA_G
Cuts at: 148 148
Size: 4517
XmaI C'CCGG_G
Cuts at: 782 1537 782
Size: 755 3762
XmnI GAAnn'nnTTC
Cuts at: 757 1156 757
Size: 399 4118
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PsiI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BpmI Bpu10I BsiWI BsmBI BspEI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI