Restriction map for
pF1KB9554
(Circular) (Six-base) MAPSORT of: pF1KB9554.seq Check: 3390 from: 1 to: 3623
pF1KB9554 3623 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 661 661
Size: 3623
Acc65I G'GTAC_C
Cuts at: 639 639
Size: 3623
AclI AA'CG_TT
Cuts at: 3463 3463
Size: 3623
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1448 1880 2233 2895 1448
Size: 432 353 662 2176
Fragments arranged by size:
2176 662 432 353
AflIII A'CryG_T
Cuts at: 224 335 975 2775 3056 224
Size: 111 640 1800 281 791
Fragments arranged by size:
1800 791 640 281 111
AlwNI CAG_nnn'CTG
Cuts at: 360 552 928 2366 360
Size: 192 376 1438 1617
Fragments arranged by size:
1617 1438 376 192
ApaLI G'TGCA_C
Cuts at: 2461 2461
Size: 3623
ApoI r'AATT_y
Cuts at: 627 2976 3232 627
Size: 2349 256 1018
Fragments arranged by size:
2349 1018 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3623
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3623
AvaI C'yCGr_G
Cuts at: 424 643 424
Size: 219 3404
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 111 144 111
Size: 33 3590
BamHI G'GATC_C
Cuts at: 648 648
Size: 3623
BanI G'GyrC_C
Cuts at: 570 639 1053 1301 1336 570
Size: 69 414 248 35 2857
Fragments arranged by size:
2857 414 248 69 35
BanII G_rGCy'C
Cuts at: 422 475 637 1667 422
Size: 53 162 1030 2378
Fragments arranged by size:
2378 1030 162 53
BbeI G_GCGC'C
Cuts at: 1057 1305 1057
Size: 248 3375
BbsI GAAGACnn'nnnn_
Cuts at: 507 507
Size: 3623
BciVI GTATCCnnnnn_n'
Cuts at: 1521 2577 3623 1521
Size: 1056 1046 1521
Fragments arranged by size:
1521 1056 1046
BclI T'GATC_A
Cuts at: 1143 1143
Size: 3623
BglII A'GATC_T
Cuts at: 1138 3175 1138
Size: 2037 1586
BlpI GC'TnA_GC
Cuts at: 361 469 474 735 361
Size: 108 5 261 3249
Fragments arranged by size:
3249 261 108 5
Bme1580I G_kGCm'C
Cuts at: 573 1248 1341 2465 573
Size: 675 93 1124 1731
Fragments arranged by size:
1731 1124 675 93
BmrI ACTGGGnnnn_n'
Cuts at: 835 916 1251 3395 835
Size: 81 335 2144 1063
Fragments arranged by size:
2144 1063 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1990 1990
Size: 3623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 469 457
Size: 12 3611
Bpu10I CC'TnA_GC
Cuts at: 416 416
Size: 3623
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 799 1678 2392 2690 184
Size: 615 879 714 298 1117
Fragments arranged by size:
1117 879 714 615 298
BsaI GGTCTCn'nnnn_
Cuts at: 341 3327 341
Size: 2986 637
BsaAI yAC'GTr
Cuts at: 976 976
Size: 3623
BsaBI GATnn'nnATC
Cuts at: 6 1137 3174 6
Size: 1131 2037 455
Fragments arranged by size:
2037 1131 455
BsaHI Gr'CG_yC
Cuts at: 1054 1302 2004 3500 1054
Size: 248 702 1496 1177
Fragments arranged by size:
1496 1177 702 248
BsaWI w'CCGG_w
Cuts at: 812 1333 2011 2422 2569 2843 812
Size: 521 678 411 147 274 1592
Fragments arranged by size:
1592 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 298 328 298
Size: 30 3593
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 394 391
Size: 3 3620
BseYI C'CCAG_C
Cuts at: 1048 1806 2471 2879 1048
Size: 758 665 408 1792
Fragments arranged by size:
1792 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 141 1165 1211 2441 104
Size: 37 1024 46 1230 1286
Fragments arranged by size:
1286 1230 1024 46 37
BsiHKAI G_wGCw'C
Cuts at: 422 475 637 1415 1605 2465 422
Size: 53 162 778 190 860 1580
Fragments arranged by size:
1580 860 778 190 162 53
Bsp1286I G_dGCh'C
Cuts at: 422 475 573 637 1248 1341 1415 1605 1667
Size: 53 98 64 611 93 74 190 62
Cuts at: 1667 2465 422
Size: 798 1580
Fragments arranged by size:
1580 798 611 190 98 93 74 64
62 53
BspHI T'CATG_A
Cuts at: 2055 2055
Size: 3623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 359 673 887 1189 1570 359
Size: 314 214 302 381 2412
Fragments arranged by size:
2412 381 314 302 214
BsrBI CCG'CTC
Cuts at: 50 143 1915 1969 3453 3618 50
Size: 93 1772 54 1484 165 55
Fragments arranged by size:
1772 1484 165 93 55 54
BsrDI GCAATG_nn'
Cuts at: 1535 1535
Size: 3623
BsrFI r'CCGG_y
Cuts at: 1621 1802 1621
Size: 181 3442
BssSI C'ACGA_G
Cuts at: 1894 2602 1894
Size: 708 2915
BstAPI GCAn_nnn'nTGC
Cuts at: 1000 1000
Size: 3623
BstBI TT'CG_AA
Cuts at: 1984 1984
Size: 3623
BstXI CCAn_nnnn'nTGG
Cuts at: 252 252
Size: 3623
BstYI r'GATC_y
Cuts at: 1 648 1115 1138 1473 1719 2025 2037 2123
Size: 647 467 23 335 246 306 12 86
Cuts at: 2123 3175 1
Size: 1052 449
Fragments arranged by size:
1052 647 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 527 107
Size: 420 3203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1573 1754 1917 1573
Size: 181 163 3279
Fragments arranged by size:
3279 181 163
BtsI GCAGTG_nn'
Cuts at: 866 866
Size: 3623
DraI TTT'AAA
Cuts at: 622 622
Size: 3623
DrdI GACnn_nn'nnGTC
Cuts at: 1329 2673 1329
Size: 1344 2279
EaeI y'GGCC_r
Cuts at: 535 1208 1382 1773 1800 3145 535
Size: 673 174 391 27 1345 1013
Fragments arranged by size:
1345 1013 673 391 174 27
EagI C'GGCC_G
Cuts at: 1208 1208
Size: 3623
EarI CTCTTCn'nnn_
Cuts at: 448 1646 1856 448
Size: 1198 210 2215
Fragments arranged by size:
2215 1198 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1831 2573 2719 3433 1831
Size: 742 146 714 2021
Fragments arranged by size:
2021 742 714 146
EcoICRI GAG'CTC
Cuts at: 420 473 635 420
Size: 53 162 3408
Fragments arranged by size:
3408 162 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 469 1448 1880 2233 2895 457
Size: 12 979 432 353 662 1185
Fragments arranged by size:
1185 979 662 432 353 12
EcoO109I rG'GnC_Cy
Cuts at: 762 762
Size: 3623
EcoRI G'AATT_C
Cuts at: 627 627
Size: 3623
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 138 1642 138
Size: 1504 2119
FspI TGC'GCA
Cuts at: 1404 1404
Size: 3623
HaeII r_GCGC'y
Cuts at: 1057 1305 2535 3306 1057
Size: 248 1230 771 1374
Fragments arranged by size:
1374 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 1465 1495 1497 1527 298
Size: 32 1135 30 2 30 2394
Fragments arranged by size:
2394 1135 32 30 30 2
HincII GTy'rAC
Cuts at: 662 662
Size: 3623
KasI G'GCGC_C
Cuts at: 1053 1301 1053
Size: 248 3375
KpnI G_GTAC'C
Cuts at: 643 643
Size: 3623
MluI A'CGCG_T
Cuts at: 3056 3056
Size: 3623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 341 2382 2566 341
Size: 2041 184 1398
Fragments arranged by size:
2041 1398 184
MscI TGG'CCA
Cuts at: 537 1384 537
Size: 847 2776
MslI CAynn'nnrTG
Cuts at: 370 1612 1739 370
Size: 1242 127 2254
Fragments arranged by size:
2254 1242 127
MspA1I CmG'CkG
Cuts at: 241 734 1048 1408 2192 2437 241
Size: 493 314 360 784 245 1427
Fragments arranged by size:
1427 784 493 360 314 245
NaeI GCC'GGC
Cuts at: 1804 1804
Size: 3623
NarI GG'CG_CC
Cuts at: 1054 1302 1054
Size: 248 3375
NcoI C'CATG_G
Cuts at: 107 107
Size: 3623
NgoMIV G'CCGG_C
Cuts at: 1802 1802
Size: 3623
NspI r_CATG'y
Cuts at: 228 339 676 1176 2779 228
Size: 111 337 500 1603 1072
Fragments arranged by size:
1603 1072 500 337 111
PciI A'CATG_T
Cuts at: 224 335 2775 224
Size: 111 2440 1072
Fragments arranged by size:
2440 1072 111
PflMI CCAn_nnn'nTGG
Cuts at: 398 398
Size: 3623
PfoI T'CCnGG_A
Cuts at: 149 149
Size: 3623
PmeI GTTT'AAAC
Cuts at: 622 622
Size: 3623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2069 2101 2069
Size: 32 3591
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 211 243 211
Size: 32 3591
PstI C_TGCA'G
Cuts at: 194 280 325 356 599 670 1355 194
Size: 86 45 31 243 71 685 2462
Fragments arranged by size:
2462 685 243 86 71 45 31
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3623
PvuII CAG'CTG
Cuts at: 241 1048 1408 241
Size: 807 360 2456
Fragments arranged by size:
2456 807 360
RsrII CG'GwC_CG
Cuts at: 1818 1818
Size: 3623
SacI G_AGCT'C
Cuts at: 422 475 637 422
Size: 53 162 3408
Fragments arranged by size:
3408 162 53
SalI G'TCGA_C
Cuts at: 660 660
Size: 3623
SapI GCTCTTCn'nnn_
Cuts at: 1646 1856 1646
Size: 210 3413
SbfI CC_TGCA'GG
Cuts at: 280 356 670 280
Size: 76 314 3233
Fragments arranged by size:
3233 314 76
ScaI AGT'ACT
Cuts at: 3140 3140
Size: 3623
SfcI C'TryA_G
Cuts at: 33 190 276 321 352 595 666 1351 2319
Size: 157 86 45 31 243 71 685 968
Cuts at: 2319 2510 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 243 191 157 86 71
45 31
SfoI GGC'GCC
Cuts at: 1055 1303 1055
Size: 248 3375
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3623
SmaI CCC'GGG
Cuts at: 645 645
Size: 3623
SmlI C'TyrA_G
Cuts at: 199 778 1693 2407 2669 199
Size: 579 915 714 262 1153
Fragments arranged by size:
1153 915 714 579 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3623
SphI G_CATG'C
Cuts at: 676 1176 676
Size: 500 3123
StyI C'CwwG_G
Cuts at: 107 757 1111 107
Size: 650 354 2619
Fragments arranged by size:
2619 650 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1206 1339 1798 2007 1206
Size: 133 459 209 2822
Fragments arranged by size:
2822 459 209 133
TatI w'GTAC_w
Cuts at: 3138 3138
Size: 3623
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 3591
Tth111I GACn'n_nGTC
Cuts at: 1420 1420
Size: 3623
XbaI T'CTAG_A
Cuts at: 654 654
Size: 3623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 307 584 1118 307
Size: 277 534 2812
Fragments arranged by size:
2812 534 277
XmaI C'CCGG_G
Cuts at: 643 643
Size: 3623
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BbvCI BcgI BfrBI BglI
BmgBI BmtI BsgI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI