Restriction map for
pF1KB9555
(Circular) (Six-base) MAPSORT of: pF1KB9555.seq Check: 5159 from: 1 to: 4451
pF1KB9555 4451 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 578 662 1294 578
Size: 84 632 3735
Fragments arranged by size:
3735 632 84
AccI GT'mk_AC
Cuts at: 1489 1489
Size: 4451
Acc65I G'GTAC_C
Cuts at: 1467 1467
Size: 4451
AclI AA'CG_TT
Cuts at: 4291 4291
Size: 4451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 537 621 1041 1125 2276 2708 3061 3723
Size: 338 84 420 84 1151 432 353 662
Cuts at: 3723 199
Size: 927
Fragments arranged by size:
1151 927 662 432 420 353 338 84
84
AflIII A'CryG_T
Cuts at: 1364 1803 3603 3884 1364
Size: 439 1800 281 1931
Fragments arranged by size:
1931 1800 439 281
AgeI A'CCGG_T
Cuts at: 1183 1313 1183
Size: 130 4321
AleI CACnn'nnGTG
Cuts at: 1184 1184
Size: 4451
AlwNI CAG_nnn'CTG
Cuts at: 1756 3194 1756
Size: 1438 3013
ApaLI G'TGCA_C
Cuts at: 3289 3289
Size: 4451
ApoI r'AATT_y
Cuts at: 303 839 1455 3804 4060 303
Size: 536 616 2349 256 694
Fragments arranged by size:
2349 694 616 536 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4451
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
AvaI C'yCGr_G
Cuts at: 1471 1471
Size: 4451
BamHI G'GATC_C
Cuts at: 1007 1476 1007
Size: 469 3982
BanI G'GyrC_C
Cuts at: 662 1310 1467 1881 2129 2164 662
Size: 648 157 414 248 35 2949
Fragments arranged by size:
2949 648 414 248 157 35
BanII G_rGCy'C
Cuts at: 377 443 565 649 733 817 901 1069 1153
Size: 66 122 84 84 84 84 168 84
Cuts at: 1153 1402 1465 2495 377
Size: 249 63 1030 2333
Fragments arranged by size:
2333 1030 249 168 122 84 84 84
84 84 66 63
BbeI G_GCGC'C
Cuts at: 1885 2133 1885
Size: 248 4203
BbvCI CC'TCA_GC
Cuts at: 409 409
Size: 4451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1393 1427 1393
Size: 34 4417
BciVI GTATCCnnnnn_n'
Cuts at: 352 2349 3405 4451 352
Size: 1997 1056 1046 352
Fragments arranged by size:
1997 1056 1046 352
BclI T'GATC_A
Cuts at: 1971 1971
Size: 4451
BglII A'GATC_T
Cuts at: 1966 4003 1966
Size: 2037 2414
BlpI GC'TnA_GC
Cuts at: 732 1563 732
Size: 831 3620
Bme1580I G_kGCm'C
Cuts at: 2076 2169 3293 2076
Size: 93 1124 3234
Fragments arranged by size:
3234 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 437 605 1025 1663 1744 2079 4223 437
Size: 168 420 638 81 335 2144 665
Fragments arranged by size:
2144 665 638 420 335 168 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 4451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 801 379
Size: 422 4029
Bpu10I CC'TnA_GC
Cuts at: 409 658 900 907 409
Size: 249 242 7 3953
Fragments arranged by size:
3953 249 242 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 1627 2506 3220 3518 303
Size: 1324 879 714 298 1236
Fragments arranged by size:
1324 1236 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 133 849 4155 133
Size: 716 3306 429
Fragments arranged by size:
3306 716 429
BsaAI yAC'GTr
Cuts at: 1365 1804 1365
Size: 439 4012
BsaBI GATnn'nnATC
Cuts at: 6 1965 4002 6
Size: 1959 2037 455
Fragments arranged by size:
2037 1959 455
BsaHI Gr'CG_yC
Cuts at: 1882 2130 2832 4328 1882
Size: 248 702 1496 2005
Fragments arranged by size:
2005 1496 702 248
BsaWI w'CCGG_w
Cuts at: 763 1183 1313 1640 2161 2839 3250 3397 3671
Size: 420 130 327 521 678 411 147 274
Cuts at: 3671 763
Size: 1543
Fragments arranged by size:
1543 678 521 420 411 327 274 147
130
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1143 1143
Size: 4451
BseYI C'CCAG_C
Cuts at: 120 1876 2634 3299 3707 120
Size: 1756 758 665 408 864
Fragments arranged by size:
1756 864 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 479 804 479
Size: 325 4126
BsiEI CG_ry'CG
Cuts at: 104 1993 2039 3269 104
Size: 1889 46 1230 1286
Fragments arranged by size:
1889 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 319 377 565 649 733 817 901 1069 1085
Size: 58 188 84 84 84 84 168 16
Cuts at: 1085 1153 1169 1334 1402 1465 2243 2433 3293
Size: 68 16 165 68 63 778 190 860
Cuts at: 3293 319
Size: 1477
Fragments arranged by size:
1477 860 778 190 188 168 165 84
84 84 84 68 68 63 58 16
16
BsmI GAATG_Cn'
Cuts at: 796 796
Size: 4451
Bsp1286I G_dGCh'C
Cuts at: 319 377 443 565 649 733 817 901 1069
Size: 58 66 122 84 84 84 84 168
Cuts at: 1069 1085 1153 1169 1334 1402 1465 2076 2169
Size: 16 68 16 165 68 63 611 93
Cuts at: 2169 2243 2433 2495 3293 319
Size: 74 190 62 798 1477
Fragments arranged by size:
1477 798 611 190 168 165 122 93
84 84 84 84 74 68 68 66
63 62 58 16 16
BspHI T'CATG_A
Cuts at: 2883 2883
Size: 4451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 578 662 1294 1501 1715 2017 2398 578
Size: 84 632 207 214 302 381 2631
Fragments arranged by size:
2631 632 381 302 214 207 84
BsrBI CCG'CTC
Cuts at: 50 2743 2797 4281 4446 50
Size: 2693 54 1484 165 55
Fragments arranged by size:
2693 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2363 2363
Size: 4451
BsrFI r'CCGG_y
Cuts at: 1183 1267 1313 2449 2630 1183
Size: 84 46 1136 181 3004
Fragments arranged by size:
3004 1136 181 84 46
BssSI C'ACGA_G
Cuts at: 832 946 2722 3430 832
Size: 114 1776 708 1853
Fragments arranged by size:
1853 1776 708 114
BstAPI GCAn_nnn'nTGC
Cuts at: 791 1376 1828 791
Size: 585 452 3414
Fragments arranged by size:
3414 585 452
BstBI TT'CG_AA
Cuts at: 2812 2812
Size: 4451
BstXI CCAn_nnnn'nTGG
Cuts at: 1129 1129
Size: 4451
BstYI r'GATC_y
Cuts at: 1 257 755 923 1007 1476 1943 1966 2301
Size: 256 498 168 84 469 467 23 335
Cuts at: 2301 2547 2853 2865 2951 4003 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 498 469 467 449 335 306 256
246 168 86 84 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4451
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1052 2401 2582 2745 1052
Size: 1349 181 163 2758
Fragments arranged by size:
2758 1349 181 163
BtsI GCAGTG_nn'
Cuts at: 1694 1694
Size: 4451
DraI TTT'AAA
Cuts at: 1450 1450
Size: 4451
DraIII CAC_nnn'GTG
Cuts at: 585 585
Size: 4451
DrdI GACnn_nn'nnGTC
Cuts at: 2157 3501 2157
Size: 1344 3107
EaeI y'GGCC_r
Cuts at: 2036 2210 2601 2628 3973 2036
Size: 174 391 27 1345 2514
Fragments arranged by size:
2514 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2036 2036
Size: 4451
EarI CTCTTCn'nnn_
Cuts at: 382 431 2474 2684 382
Size: 49 2043 210 2149
Fragments arranged by size:
2149 2043 210 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 712 2659 3401 3547 4261 712
Size: 1947 742 146 714 902
Fragments arranged by size:
1947 902 742 714 146
EcoICRI GAG'CTC
Cuts at: 375 563 647 731 815 899 1067 1151 1400
Size: 188 84 84 84 84 168 84 249
Cuts at: 1400 1463 375
Size: 63 3363
Fragments arranged by size:
3363 249 188 168 84 84 84 84
84 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 379 537 621 801 1041 1125 2276 2708
Size: 180 158 84 180 240 84 1151 432
Cuts at: 2708 3061 3723 199
Size: 353 662 927
Fragments arranged by size:
1151 927 662 432 353 240 180 180
158 84 84
EcoO109I rG'GnC_Cy
Cuts at: 195 204 484 773 1590 195
Size: 9 280 289 817 3056
Fragments arranged by size:
3056 817 289 280 9
EcoRI G'AATT_C
Cuts at: 839 1455 839
Size: 616 3835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4451
FspI TGC'GCA
Cuts at: 2232 2232
Size: 4451
HaeII r_GCGC'y
Cuts at: 1885 2133 3363 4134 1885
Size: 248 1230 771 2202
Fragments arranged by size:
2202 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 846 878 900 932 1401 1433 2293 2323 2325
Size: 32 22 32 469 32 860 30 2
Cuts at: 2325 2355 846
Size: 30 2942
Fragments arranged by size:
2942 860 469 32 32 32 30 30
22 2
HincII GTy'rAC
Cuts at: 1490 1490
Size: 4451
KasI G'GCGC_C
Cuts at: 1881 2129 1881
Size: 248 4203
KpnI G_GTAC'C
Cuts at: 1471 1471
Size: 4451
MluI A'CGCG_T
Cuts at: 3884 3884
Size: 4451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 428 600 1020 1094 3210 3394 428
Size: 172 420 74 2116 184 1485
Fragments arranged by size:
2116 1485 420 184 172 74
MscI TGG'CCA
Cuts at: 2212 2212
Size: 4451
MslI CAynn'nnrTG
Cuts at: 1032 1184 2440 2567 1032
Size: 152 1256 127 2916
Fragments arranged by size:
2916 1256 152 127
MspA1I CmG'CkG
Cuts at: 1005 1562 1876 2236 3020 3265 1005
Size: 557 314 360 784 245 2191
Fragments arranged by size:
2191 784 557 360 314 245
NaeI GCC'GGC
Cuts at: 2632 2632
Size: 4451
NarI GG'CG_CC
Cuts at: 1882 2130 1882
Size: 248 4203
NcoI C'CATG_G
Cuts at: 107 107
Size: 4451
NgoMIV G'CCGG_C
Cuts at: 2630 2630
Size: 4451
NspI r_CATG'y
Cuts at: 417 1504 2004 3607 417
Size: 1087 500 1603 1261
Fragments arranged by size:
1603 1261 1087 500
PasI CC'CwG_GG
Cuts at: 187 187
Size: 4451
PciI A'CATG_T
Cuts at: 3603 3603
Size: 4451
PmeI GTTT'AAAC
Cuts at: 1450 1450
Size: 4451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2897 2929 2897
Size: 32 4419
PpuMI rG'GwC_Cy
Cuts at: 195 204 484 195
Size: 9 280 4162
Fragments arranged by size:
4162 280 9
PstI C_TGCA'G
Cuts at: 1498 2183 1498
Size: 685 3766
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4451
PvuII CAG'CTG
Cuts at: 1876 2236 1876
Size: 360 4091
RsrII CG'GwC_CG
Cuts at: 2646 2646
Size: 4451
SacI G_AGCT'C
Cuts at: 377 565 649 733 817 901 1069 1153 1402
Size: 188 84 84 84 84 168 84 249
Cuts at: 1402 1465 377
Size: 63 3363
Fragments arranged by size:
3363 249 188 168 84 84 84 84
84 63
SalI G'TCGA_C
Cuts at: 1488 1488
Size: 4451
SanDI GG'GwC_CC
Cuts at: 204 204
Size: 4451
SapI GCTCTTCn'nnn_
Cuts at: 382 431 2474 2684 382
Size: 49 2043 210 2149
Fragments arranged by size:
2149 2043 210 49
SbfI CC_TGCA'GG
Cuts at: 1498 1498
Size: 4451
ScaI AGT'ACT
Cuts at: 3968 3968
Size: 4451
SexAI A'CCwGG_T
Cuts at: 581 581
Size: 4451
SfcI C'TryA_G
Cuts at: 33 1494 2179 3147 3338 33
Size: 1461 685 968 191 1146
Fragments arranged by size:
1461 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1883 2131 1883
Size: 248 4203
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
SgrAI Cr'CCGG_yG
Cuts at: 1183 1183
Size: 4451
SmaI CCC'GGG
Cuts at: 1473 1473
Size: 4451
SmlI C'TyrA_G
Cuts at: 318 1606 2521 3235 3497 318
Size: 1288 915 714 262 1272
Fragments arranged by size:
1288 1272 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4451
SphI G_CATG'C
Cuts at: 1504 2004 1504
Size: 500 3951
StuI AGG'CCT
Cuts at: 550 634 718 905 1054 1222 550
Size: 84 84 187 149 168 3779
Fragments arranged by size:
3779 187 168 149 84 84
StyI C'CwwG_G
Cuts at: 107 1585 1939 107
Size: 1478 354 2619
Fragments arranged by size:
2619 1478 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2034 2167 2626 2835 2034
Size: 133 459 209 3650
Fragments arranged by size:
3650 459 209 133
TatI w'GTAC_w
Cuts at: 3966 3966
Size: 4451
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 977 1009 3968 4000 977
Size: 32 2959 32 1428
Fragments arranged by size:
2959 1428 32 32
Tth111I GACn'n_nGTC
Cuts at: 2248 2248
Size: 4451
XbaI T'CTAG_A
Cuts at: 1482 1482
Size: 4451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1946 1946
Size: 4451
XmaI C'CCGG_G
Cuts at: 1471 1471
Size: 4451
XmnI GAAnn'nnTTC
Cuts at: 550 634 718 802 886 1054 1138 550
Size: 84 84 84 84 168 84 3863
Fragments arranged by size:
3863 168 84 84 84 84 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmBI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SfiI SnaBI SrfI SspI SwaI XhoI ZraI