Restriction map for
pF1KB9557
(Circular) (Six-base) MAPSORT of: pF1KB9557.seq Check: 6609 from: 1 to: 9125
pF1KB9557 9125 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4728 4728
Size: 9125
AatII G_ACGT'C
Cuts at: 1119 2782 1119
Size: 1663 7462
AccI GT'mk_AC
Cuts at: 1038 6163 1038
Size: 5125 4000
Acc65I G'GTAC_C
Cuts at: 6141 6141
Size: 9125
AclI AA'CG_TT
Cuts at: 474 2739 8965 474
Size: 2265 6226 634
Fragments arranged by size:
6226 2265 634
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 1149 1439 2371 2768 3001 3042 3157 3254
Size: 976 290 932 397 233 41 115 97
Cuts at: 3254 3836 4740 5343 5575 6950 7382 7735 8397
Size: 582 904 603 232 1375 432 353 662
Cuts at: 8397 173
Size: 901
Fragments arranged by size:
1375 976 932 904 901 662 603 582
432 397 353 290 233 232 115 97
41
AflIII A'CryG_T
Cuts at: 2638 4250 5600 6477 8277 8558 2638
Size: 1612 1350 877 1800 281 3205
Fragments arranged by size:
3205 1800 1612 1350 877 281
AleI CACnn'nnGTG
Cuts at: 3235 4255 3235
Size: 1020 8105
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4978 4978
Size: 9125
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4837 4869 4837
Size: 32 9093
AlwNI CAG_nnn'CTG
Cuts at: 445 908 4030 4473 4838 4952 5275 5382 5727
Size: 463 3122 443 365 114 323 107 345
Cuts at: 5727 6430 7868 445
Size: 703 1438 1702
Fragments arranged by size:
3122 1702 1438 703 463 443 365 345
323 114 107
ApaLI G'TGCA_C
Cuts at: 7963 7963
Size: 9125
ApoI r'AATT_y
Cuts at: 1351 1661 1729 2394 3510 6129 8478 8734 1351
Size: 310 68 665 1116 2619 2349 256 1742
Fragments arranged by size:
2619 2349 1742 1116 665 310 256 68
AseI AT'TA_AT
Cuts at: 20 20
Size: 9125
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9125
AvaI C'yCGr_G
Cuts at: 1385 3766 6145 1385
Size: 2381 2379 4365
Fragments arranged by size:
4365 2381 2379
AvrII C'CTAG_G
Cuts at: 1042 1042
Size: 9125
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5280 5313 5280
Size: 33 9092
BamHI G'GATC_C
Cuts at: 4657 6150 4657
Size: 1493 7632
BanI G'GyrC_C
Cuts at: 4429 5326 6141 6555 6803 6838 4429
Size: 897 815 414 248 35 6716
Fragments arranged by size:
6716 897 815 414 248 35
BanII G_rGCy'C
Cuts at: 1334 2945 3680 6139 7169 1334
Size: 1611 735 2459 1030 3290
Fragments arranged by size:
3290 2459 1611 1030 735
BbeI G_GCGC'C
Cuts at: 4433 6559 6807 4433
Size: 2126 248 6751
Fragments arranged by size:
6751 2126 248
BbsI GAAGACnn'nnnn_
Cuts at: 209 2665 2774 2928 3365 3758 4307 4529 209
Size: 2456 109 154 437 393 549 222 4805
Fragments arranged by size:
4805 2456 549 437 393 222 154 109
BbvCI CC'TCA_GC
Cuts at: 2489 5162 2489
Size: 2673 6452
BciVI GTATCCnnnnn_n'
Cuts at: 5963 7023 8079 9125 5963
Size: 1060 1056 1046 5963
Fragments arranged by size:
5963 1060 1056 1046
BclI T'GATC_A
Cuts at: 5255 6645 5255
Size: 1390 7735
BglI GCCn_nnn'nGGC
Cuts at: 5761 5761
Size: 9125
BglII A'GATC_T
Cuts at: 6640 8677 6640
Size: 2037 7088
BlpI GC'TnA_GC
Cuts at: 5483 6237 5483
Size: 754 8371
Bme1580I G_kGCm'C
Cuts at: 5669 6086 6750 6843 7967 5669
Size: 417 664 93 1124 6827
Fragments arranged by size:
6827 1124 664 417 93
BmgBI CAC'GTC
Cuts at: 4253 4253
Size: 9125
BmrI ACTGGGnnnn_n'
Cuts at: 5033 5294 6337 6418 6753 8897 5033
Size: 261 1043 81 335 2144 5261
Fragments arranged by size:
5261 2144 1043 335 261 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2974 7492 2974
Size: 4518 4607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 659 2126 2423 2609 3851 4099 4875 659
Size: 1467 297 186 1242 248 776 4909
Fragments arranged by size:
4909 1467 1242 776 297 248 186
Bpu10I CC'TnA_GC
Cuts at: 356 2489 5162 5505 356
Size: 2133 2673 343 3976
Fragments arranged by size:
3976 2673 2133 343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1676 1686 2264 2944 3541 3644 4388 4642 5504
Size: 10 578 680 597 103 744 254 862
Cuts at: 5504 6301 7180 7894 8192 1676
Size: 797 879 714 298 2609
Fragments arranged by size:
2609 879 862 797 744 714 680 597
578 298 254 103 10
BsaI GGTCTCn'nnnn_
Cuts at: 2144 2469 3220 3886 4684 8829 2144
Size: 325 751 666 798 4145 2440
Fragments arranged by size:
4145 2440 798 751 666 325
BsaAI yAC'GTr
Cuts at: 6478 6478
Size: 9125
BsaBI GATnn'nnATC
Cuts at: 6 2414 6639 8676 6
Size: 2408 4225 2037 455
Fragments arranged by size:
4225 2408 2037 455
BsaHI Gr'CG_yC
Cuts at: 1116 2779 4430 5539 6556 6804 7506 9002 1116
Size: 1663 1651 1109 1017 248 702 1496 1239
Fragments arranged by size:
1663 1651 1496 1239 1109 1017 702 248
BsaWI w'CCGG_w
Cuts at: 6314 6835 7513 7924 8071 8345 6314
Size: 521 678 411 147 274 7094
Fragments arranged by size:
7094 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3140 3170 5232 5262 3140
Size: 30 2062 30 7003
Fragments arranged by size:
7003 2062 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1522 1668 2485 2497 2780 2893 3088 3091 3094
Size: 146 817 12 283 113 195 3 3
Cuts at: 3094 3097 3183 3334 3506 3784 3796 3799 3802
Size: 3 86 151 172 278 12 3 3
Cuts at: 3802 3934 3964 3982 3985 4003 4018 4021 4150
Size: 132 30 18 3 18 15 3 129
Cuts at: 4150 4246 4354 4548 5507 5573 1522
Size: 96 108 194 959 66 5074
Fragments arranged by size:
5074 959 817 283 278 195 194 172
151 146 132 129 113 108 96 86
66 30 18 18 15 12 12 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 1484 2708 3855 4276 4576 5132 5168 5195 5244
Size: 1224 1147 421 300 556 36 27 49
Cuts at: 5244 5509 5999 6550 7308 7973 8381 1484
Size: 265 490 551 758 665 408 2228
Fragments arranged by size:
2228 1224 1147 758 665 556 551 490
421 408 300 265 49 36 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3006 4411 3006
Size: 1405 7720
BsiEI CG_ry'CG
Cuts at: 104 6667 6713 7943 104
Size: 6563 46 1230 1286
Fragments arranged by size:
6563 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 4783 6139 6917 7107 7967 4783
Size: 1356 778 190 860 5941
Fragments arranged by size:
5941 1356 860 778 190
BsmI GAATG_Cn'
Cuts at: 2318 5621 5871 2318
Size: 3303 250 5572
Fragments arranged by size:
5572 3303 250
BsmBI CGTCTCn'nnnn_
Cuts at: 2770 2770
Size: 9125
Bsp1286I G_dGCh'C
Cuts at: 1334 2945 3680 4783 5669 6086 6139 6750 6843
Size: 1611 735 1103 886 417 53 611 93
Cuts at: 6843 6917 7107 7169 7967 1334
Size: 74 190 62 798 2492
Fragments arranged by size:
2492 1611 1103 886 798 735 611 417
190 93 74 62 53
BspHI T'CATG_A
Cuts at: 1535 2562 2664 7557 1535
Size: 1027 102 4893 3103
Fragments arranged by size:
4893 3103 1027 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2075 4728 6175 6389 6691 7072 2075
Size: 2653 1447 214 302 381 4128
Fragments arranged by size:
4128 2653 1447 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1263 5681 7417 7471 8955 9120 50
Size: 1213 4418 1736 54 1484 165 55
Fragments arranged by size:
4418 1736 1484 1213 165 55 54
BsrDI GCAATG_nn'
Cuts at: 213 1302 7037 213
Size: 1089 5735 2301
Fragments arranged by size:
5735 2301 1089
BsrFI r'CCGG_y
Cuts at: 708 5329 7123 7304 708
Size: 4621 1794 181 2529
Fragments arranged by size:
4621 2529 1794 181
BsrGI T'GTAC_A
Cuts at: 4380 6038 4380
Size: 1658 7467
BssSI C'ACGA_G
Cuts at: 4116 7396 8104 4116
Size: 3280 708 5137
Fragments arranged by size:
5137 3280 708
BstAPI GCAn_nnn'nTGC
Cuts at: 4030 4952 5640 6009 6502 4030
Size: 922 688 369 493 6653
Fragments arranged by size:
6653 922 688 493 369
BstBI TT'CG_AA
Cuts at: 1176 7486 1176
Size: 6310 2815
BstXI CCAn_nnnn'nTGG
Cuts at: 1993 2460 3593 1993
Size: 467 1133 7525
Fragments arranged by size:
7525 1133 467
BstYI r'GATC_y
Cuts at: 1 324 2415 3716 4368 4657 6150 6617 6640
Size: 323 2091 1301 652 289 1493 467 23
Cuts at: 6640 6975 7221 7527 7539 7625 8677 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
2091 1493 1301 1052 652 467 449 335
323 306 289 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2792 2972 3049 3911 2792
Size: 180 77 862 8006
Fragments arranged by size:
8006 862 180 77
BtgI C'CryG_G
Cuts at: 107 225 369 576 4256 4514 107
Size: 118 144 207 3680 258 4718
Fragments arranged by size:
4718 3680 258 207 144 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7075 7256 7419 7075
Size: 181 163 8781
Fragments arranged by size:
8781 181 163
BtsI GCAGTG_nn'
Cuts at: 525 886 1582 2016 3207 3852 4394 4422 5695
Size: 361 696 434 1191 645 542 28 1273
Cuts at: 5695 6368 525
Size: 673 3282
Fragments arranged by size:
3282 1273 1191 696 673 645 542 434
361 28
ClaI AT'CG_AT
Cuts at: 2096 3308 2096
Size: 1212 7913
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 614 649 5598 5633 614
Size: 35 4949 35 4106
Fragments arranged by size:
4949 4106 35 35
DraI TTT'AAA
Cuts at: 290 833 6124 290
Size: 543 5291 3291
Fragments arranged by size:
5291 3291 543
DrdI GACnn_nn'nnGTC
Cuts at: 3043 6831 8175 3043
Size: 3788 1344 3993
Fragments arranged by size:
3993 3788 1344
EaeI y'GGCC_r
Cuts at: 573 816 4071 4934 5733 6710 6884 7275 7302
Size: 243 3255 863 799 977 174 391 27
Cuts at: 7302 8647 573
Size: 1345 1051
Fragments arranged by size:
3255 1345 1051 977 863 799 391 243
174 27
EagI C'GGCC_G
Cuts at: 6710 6710
Size: 9125
EarI CTCTTCn'nnn_
Cuts at: 2474 2501 2768 3311 3320 3408 3434 3803 3812
Size: 27 267 543 9 88 26 369 9
Cuts at: 3812 3824 3923 4174 4223 4313 4340 4857 6111
Size: 12 99 251 49 90 27 517 1254
Cuts at: 6111 7148 7358 2474
Size: 1037 210 4241
Fragments arranged by size:
4241 1254 1037 543 517 369 267 251
210 99 90 88 49 27 27 26
12 9 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 980 1392 2338 7333 8075 8221 8935 980
Size: 412 946 4995 742 146 714 1170
Fragments arranged by size:
4995 1170 946 742 714 412 146
EcoICRI GAG'CTC
Cuts at: 6137 6137
Size: 9125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 659 1149 1439 2126 2371 2423 2609 2768
Size: 486 490 290 687 245 52 186 159
Cuts at: 2768 3001 3042 3157 3254 3836 3851 4099 4740
Size: 233 41 115 97 582 15 248 641
Cuts at: 4740 4875 5343 5575 6950 7382 7735 8397 173
Size: 135 468 232 1375 432 353 662 901
Fragments arranged by size:
1375 901 687 662 641 582 490 486
468 432 353 290 248 245 233 232
186 159 135 115 97 52 41 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3766 3766
Size: 9125
EcoO109I rG'GnC_Cy
Cuts at: 1159 4500 6046 6264 1159
Size: 3341 1546 218 4020
Fragments arranged by size:
4020 3341 1546 218
EcoRI G'AATT_C
Cuts at: 3510 6129 3510
Size: 2619 6506
EcoRV GAT'ATC
Cuts at: 1569 1569
Size: 9125
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7144 7144
Size: 9125
FspI TGC'GCA
Cuts at: 6906 6906
Size: 9125
HaeII r_GCGC'y
Cuts at: 1098 4433 6559 6807 8037 8808 1098
Size: 3335 2126 248 1230 771 1415
Fragments arranged by size:
3335 2126 1415 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 542 574 2043 2075 3250 3282 3427 3459 5808
Size: 32 1469 32 1175 32 145 32 2349
Cuts at: 5808 5840 6967 6997 6999 7029 542
Size: 32 1127 30 2 30 2638
Fragments arranged by size:
2638 2349 1469 1175 1127 145 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1039 2264 6164 1039
Size: 1225 3900 4000
Fragments arranged by size:
4000 3900 1225
HindIII A'AGCT_T
Cuts at: 2383 2383
Size: 9125
KasI G'GCGC_C
Cuts at: 4429 6555 6803 4429
Size: 2126 248 6751
Fragments arranged by size:
6751 2126 248
KpnI G_GTAC'C
Cuts at: 6145 6145
Size: 9125
MfeI C'AATT_G
Cuts at: 256 256
Size: 9125
MluI A'CGCG_T
Cuts at: 8558 8558
Size: 9125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1575 2477 7884 8068 1575
Size: 902 5407 184 2632
Fragments arranged by size:
5407 2632 902 184
MscI TGG'CCA
Cuts at: 575 818 4073 4936 6886 575
Size: 243 3255 863 1950 2814
Fragments arranged by size:
3255 2814 1950 863 243
MslI CAynn'nnrTG
Cuts at: 1003 2225 3235 4255 5395 5566 5800 5851 7114
Size: 1222 1010 1020 1140 171 234 51 1263
Cuts at: 7114 7241 1003
Size: 127 2887
Fragments arranged by size:
2887 1263 1222 1140 1020 1010 234 171
127 51
MspA1I CmG'CkG
Cuts at: 1628 2391 2708 2865 4021 4751 4865 4913 4952
Size: 763 317 157 1156 730 114 48 39
Cuts at: 4952 5211 5382 5487 5727 6236 6550 6910 7694
Size: 259 171 105 240 509 314 360 784
Cuts at: 7694 7939 1628
Size: 245 2814
Fragments arranged by size:
2814 1156 784 763 730 509 360 317
314 259 245 240 171 157 114 105
48 39
NaeI GCC'GGC
Cuts at: 5331 7306 5331
Size: 1975 7150
NarI GG'CG_CC
Cuts at: 4430 6556 6804 4430
Size: 2126 248 6751
Fragments arranged by size:
6751 2126 248
NcoI C'CATG_G
Cuts at: 107 225 369 4514 107
Size: 118 144 4145 4718
Fragments arranged by size:
4718 4145 144 118
NgoMIV G'CCGG_C
Cuts at: 5329 7304 5329
Size: 1975 7150
NspI r_CATG'y
Cuts at: 902 942 1020 3668 3759 5604 6178 6678 8281
Size: 40 78 2648 91 1845 574 500 1603
Cuts at: 8281 902
Size: 1746
Fragments arranged by size:
2648 1845 1746 1603 574 500 91 78
40
PciI A'CATG_T
Cuts at: 5600 8277 5600
Size: 2677 6448
PflMI CCAn_nnn'nTGG
Cuts at: 231 5275 231
Size: 5044 4081
PfoI T'CCnGG_A
Cuts at: 2366 3719 2366
Size: 1353 7772
PmeI GTTT'AAAC
Cuts at: 6124 6124
Size: 9125
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7571 7603 7571
Size: 32 9093
PpuMI rG'GwC_Cy
Cuts at: 1159 4500 1159
Size: 3341 5784
PshAI GACnn'nnGTC
Cuts at: 2440 2440
Size: 9125
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5896 5928 6022 6054 5896
Size: 32 94 32 8967
Fragments arranged by size:
8967 94 32 32
PstI C_TGCA'G
Cuts at: 1288 1589 1769 4026 4478 4957 4999 5378 6172
Size: 301 180 2257 452 479 42 379 794
Cuts at: 6172 6857 1288
Size: 685 3556
Fragments arranged by size:
3556 2257 794 685 479 452 379 301
180 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9125
PvuII CAG'CTG
Cuts at: 2391 2865 4021 4865 4913 4952 5382 5487 5727
Size: 474 1156 844 48 39 430 105 240
Cuts at: 5727 6550 6910 2391
Size: 823 360 4606
Fragments arranged by size:
4606 1156 844 823 474 430 360 240
105 48 39
RsrII CG'GwC_CG
Cuts at: 7320 7320
Size: 9125
SacI G_AGCT'C
Cuts at: 6139 6139
Size: 9125
SalI G'TCGA_C
Cuts at: 1037 6162 1037
Size: 5125 4000
SapI GCTCTTCn'nnn_
Cuts at: 2501 4857 7148 7358 2501
Size: 2356 2291 210 4268
Fragments arranged by size:
4268 2356 2291 210
SbfI CC_TGCA'GG
Cuts at: 6172 6172
Size: 9125
ScaI AGT'ACT
Cuts at: 8642 8642
Size: 9125
SexAI A'CCwGG_T
Cuts at: 1669 3606 1669
Size: 1937 7188
SfcI C'TryA_G
Cuts at: 33 732 1284 1585 1765 2405 3024 4022 4474
Size: 699 552 301 180 640 619 998 452
Cuts at: 4474 4953 4995 5374 6168 6853 7821 8012 33
Size: 479 42 379 794 685 968 191 1146
Fragments arranged by size:
1146 998 968 794 699 685 640 619
552 479 452 379 301 191 180 42
SfoI GGC'GCC
Cuts at: 4431 6557 6805 4431
Size: 2126 248 6751
Fragments arranged by size:
6751 2126 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9125
SmaI CCC'GGG
Cuts at: 6147 6147
Size: 9125
SmlI C'TyrA_G
Cuts at: 1691 1701 2279 2959 3520 3659 4403 4621 5519
Size: 10 578 680 561 139 744 218 898
Cuts at: 5519 6280 7195 7909 8171 1691
Size: 761 915 714 262 2645
Fragments arranged by size:
2645 915 898 761 744 714 680 578
561 262 218 139 10
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9125
SphI G_CATG'C
Cuts at: 3668 6178 6678 3668
Size: 2510 500 6115
Fragments arranged by size:
6115 2510 500
SspI AAT'ATT
Cuts at: 1833 2204 1833
Size: 371 8754
StuI AGG'CCT
Cuts at: 868 5034 5992 868
Size: 4166 958 4001
Fragments arranged by size:
4166 4001 958
StyI C'CwwG_G
Cuts at: 107 225 369 1042 3902 4514 6259 6613 107
Size: 118 144 673 2860 612 1745 354 2619
Fragments arranged by size:
2860 2619 1745 673 612 354 144 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 977 2848 3089 3910 4501 4584 4651 4918 6708
Size: 1871 241 821 591 83 67 267 1790
Cuts at: 6708 6841 7300 7509 977
Size: 133 459 209 2593
Fragments arranged by size:
2593 1871 1790 821 591 459 267 241
209 133 83 67
TatI w'GTAC_w
Cuts at: 1956 2030 3384 4380 6038 8640 1956
Size: 74 1354 996 1658 2602 2441
Fragments arranged by size:
2602 2441 1658 1354 996 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2540 2572 4066 4098 5232 5264 8642 8674 2540
Size: 32 1494 32 1134 32 3378 32 2991
Fragments arranged by size:
3378 2991 1494 1134 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3608 6922 3608
Size: 3314 5811
XbaI T'CTAG_A
Cuts at: 6156 6156
Size: 9125
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1016 2740 4701 5394 5475 6620 1016
Size: 1724 1961 693 81 1145 3521
Fragments arranged by size:
3521 1961 1724 1145 693 81
XmaI C'CCGG_G
Cuts at: 6145 6145
Size: 9125
XmnI GAAnn'nnTTC
Cuts at: 1559 4349 1559
Size: 2790 6335
ZraI GAC'GTC
Cuts at: 1117 2780 1117
Size: 1663 7462
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI ApaI AscI BcgI BfrBI
BmtI BsiWI BspEI BssHII BstEII BstZ17I DraIII FseI
FspAI HpaI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PsiI PspOMI PspXI SacII SanDI SfiI
SgrAI SnaBI SrfI SwaI XhoI