Restriction map for
pF1KB9558
(Circular) (Six-base) MAPSORT of: pF1KB9558.seq Check: 5965 from: 1 to: 5162
pF1KB9558 5162 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 2200 2200
Size: 5162
Acc65I G'GTAC_C
Cuts at: 2178 2178
Size: 5162
AclI AA'CG_TT
Cuts at: 5002 5002
Size: 5162
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 731 1419 2987 3419 4166 4434 222
Size: 509 688 1568 432 747 268 950
Fragments arranged by size:
1568 950 747 688 509 432 268
AflIII A'CryG_T
Cuts at: 2514 3618 4595 2514
Size: 1104 977 3081
Fragments arranged by size:
3081 1104 977
AgeI A'CCGG_T
Cuts at: 1124 3550 1124
Size: 2426 2736
AhdI GACnn_n'nnGTC
Cuts at: 493 493
Size: 5162
AleI CACnn'nnGTG
Cuts at: 913 1478 2153 913
Size: 565 675 3922
Fragments arranged by size:
3922 675 565
AlwNI CAG_nnn'CTG
Cuts at: 189 375 417 627 1062 2467 4034 189
Size: 186 42 210 435 1405 1567 1317
Fragments arranged by size:
1567 1405 1317 435 210 186 42
ApaI G_GGCC'C
Cuts at: 121 746 828 121
Size: 625 82 4455
Fragments arranged by size:
4455 625 82
ApaLI G'TGCA_C
Cuts at: 3932 3932
Size: 5162
ApoI r'AATT_y
Cuts at: 1003 1263 1637 2166 4515 4771 1003
Size: 260 374 529 2349 256 1394
Fragments arranged by size:
2349 1394 529 374 260 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5162
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5162
AvaI C'yCGr_G
Cuts at: 120 217 272 1079 1669 2182 120
Size: 97 55 807 590 513 3100
Fragments arranged by size:
3100 807 590 513 97 55
BamHI G'GATC_C
Cuts at: 810 939 2187 810
Size: 129 1248 3785
Fragments arranged by size:
3785 1248 129
BanI G'GyrC_C
Cuts at: 907 1089 1143 1292 1544 1628 1967 2178 2592
Size: 182 54 149 252 84 339 211 414
Cuts at: 2592 2840 2875 907
Size: 248 35 3194
Fragments arranged by size:
3194 414 339 252 248 211 182 149
84 54 35
BanII G_rGCy'C
Cuts at: 121 127 146 217 746 828 976 1809 2176
Size: 6 19 71 529 82 148 833 367
Cuts at: 2176 3206 121
Size: 1030 2077
Fragments arranged by size:
2077 1030 833 529 367 148 82 71
19 6
BbeI G_GCGC'C
Cuts at: 1147 2596 2844 1147
Size: 1449 248 3465
Fragments arranged by size:
3465 1449 248
BbsI GAAGACnn'nnnn_
Cuts at: 731 1119 731
Size: 388 4774
BbvCI CC'TCA_GC
Cuts at: 283 283
Size: 5162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1203 1237 1203
Size: 34 5128
BciVI GTATCCnnnnn_n'
Cuts at: 3060 3821 5162 3060
Size: 761 1341 3060
Fragments arranged by size:
3060 1341 761
BclI T'GATC_A
Cuts at: 2682 2682
Size: 5162
BglI GCCn_nnn'nGGC
Cuts at: 980 1813 980
Size: 833 4329
BglII A'GATC_T
Cuts at: 344 1598 2677 4714 344
Size: 1254 1079 2037 792
Fragments arranged by size:
2037 1254 1079 792
BlpI GC'TnA_GC
Cuts at: 2274 2274
Size: 5162
Bme1580I G_kGCm'C
Cuts at: 121 746 828 1094 1585 2787 2880 3936 121
Size: 625 82 266 491 1202 93 1056 1347
Fragments arranged by size:
1347 1202 1056 625 491 266 93 82
BmgBI CAC'GTC
Cuts at: 771 771
Size: 5162
BmrI ACTGGGnnnn_n'
Cuts at: 741 749 884 938 1213 1571 2374 2455 2790
Size: 8 135 54 275 358 803 81 335
Cuts at: 2790 4934 741
Size: 2144 969
Fragments arranged by size:
2144 969 803 358 335 275 135 81
54 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3529 3529
Size: 5162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 198 601 816 895 974 1116 1667 181
Size: 17 403 215 79 79 142 551 3676
Fragments arranged by size:
3676 551 403 215 142 79 79 17
Bpu10I CC'TnA_GC
Cuts at: 283 970 1103 283
Size: 687 133 4342
Fragments arranged by size:
4342 687 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1173 2338 3217 3709 4007 1173
Size: 1165 879 492 298 2328
Fragments arranged by size:
2328 1165 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2070 4866 2070
Size: 2796 2366
BsaAI yAC'GTr
Cuts at: 2515 2515
Size: 5162
BsaBI GATnn'nnATC
Cuts at: 6 2676 4713 6
Size: 2670 2037 455
Fragments arranged by size:
2670 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 1144 2593 2841 3543 5039 112
Size: 1032 1449 248 702 1496 235
Fragments arranged by size:
1496 1449 1032 702 248 235
BsaWI w'CCGG_w
Cuts at: 423 1124 2351 2872 3550 3824 3971 4382 423
Size: 701 1227 521 678 274 147 411 1203
Fragments arranged by size:
1227 1203 701 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2109 2139 2109
Size: 30 5132
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 547 808 1414 178
Size: 369 261 606 3926
Fragments arranged by size:
3926 606 369 261
BseYI C'CCAG_C
Cuts at: 637 984 1107 2587 3345 3922 4418 637
Size: 347 123 1480 758 577 496 1381
Fragments arranged by size:
1480 1381 758 577 496 347 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 865 1323 430
Size: 435 458 4269
Fragments arranged by size:
4269 458 435
BsiEI CG_ry'CG
Cuts at: 104 1129 1213 2704 2750 3958 104
Size: 1025 84 1491 46 1208 1308
Fragments arranged by size:
1491 1308 1208 1025 84 46
BsiHKAI G_wGCw'C
Cuts at: 1389 2176 2954 3144 3936 1389
Size: 787 778 190 792 2615
Fragments arranged by size:
2615 792 787 778 190
Bsp1286I G_dGCh'C
Cuts at: 121 127 146 217 746 828 976 1094 1389
Size: 6 19 71 529 82 148 118 295
Cuts at: 1389 1585 1809 2176 2787 2880 2954 3144 3206
Size: 196 224 367 611 93 74 190 62
Cuts at: 3206 3936 121
Size: 730 1347
Fragments arranged by size:
1347 730 611 529 367 295 224 196
190 148 118 93 82 74 71 62
19 6
BspEI T'CCGG_A
Cuts at: 423 4382 423
Size: 3959 1203
BspHI T'CATG_A
Cuts at: 1119 1725 2007 4338 1119
Size: 606 282 2331 1943
Fragments arranged by size:
2331 1943 606 282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2212 2426 2728 3109 2212
Size: 214 302 381 4265
Fragments arranged by size:
4265 381 302 214
BsrBI CCG'CTC
Cuts at: 50 431 3454 3508 4992 5157 50
Size: 381 3023 54 1484 165 55
Fragments arranged by size:
3023 1484 381 165 55 54
BsrDI GCAATG_nn'
Cuts at: 782 3074 782
Size: 2292 2870
BsrFI r'CCGG_y
Cuts at: 287 1124 3160 3341 3550 287
Size: 837 2036 181 209 1899
Fragments arranged by size:
2036 1899 837 209 181
BsrGI T'GTAC_A
Cuts at: 779 779
Size: 5162
BssSI C'ACGA_G
Cuts at: 557 3433 3791 557
Size: 2876 358 1928
Fragments arranged by size:
2876 1928 358
BstAPI GCAn_nnn'nTGC
Cuts at: 417 627 682 1505 1869 2539 417
Size: 210 55 823 364 670 3040
Fragments arranged by size:
3040 823 670 364 210 55
BstBI TT'CG_AA
Cuts at: 3523 3523
Size: 5162
BstEII G'GTnAC_C
Cuts at: 444 444
Size: 5162
BstYI r'GATC_y
Cuts at: 1 344 395 810 939 1598 1951 2187 2654
Size: 343 51 415 129 659 353 236 467
Cuts at: 2654 2677 3012 3258 4270 4356 4368 4714 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 659 467 449 415 353 346 343
335 246 236 129 86 51 23 12
Bsu36I CC'TnA_GG
Cuts at: 1055 1055
Size: 5162
BtgI C'CryG_G
Cuts at: 107 306 504 1800 107
Size: 199 198 1296 3469
Fragments arranged by size:
3469 1296 199 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3112 3293 3456 3112
Size: 181 163 4818
Fragments arranged by size:
4818 181 163
BtsI GCAGTG_nn'
Cuts at: 2405 2405
Size: 5162
DraI TTT'AAA
Cuts at: 2161 2161
Size: 5162
DrdI GACnn_nn'nnGTC
Cuts at: 2868 3726 2868
Size: 858 4304
EaeI y'GGCC_r
Cuts at: 2747 2921 3312 3339 4684 2747
Size: 174 391 27 1345 3225
Fragments arranged by size:
3225 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2747 2747
Size: 5162
EarI CTCTTCn'nnn_
Cuts at: 992 3185 3395 992
Size: 2193 210 2759
Fragments arranged by size:
2759 2193 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3370 3680 3826 4972 3370
Size: 310 146 1146 3560
Fragments arranged by size:
3560 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2174 2174
Size: 5162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 198 222 601 731 816 895 974 1116
Size: 17 24 379 130 85 79 79 142
Cuts at: 1116 1419 1667 2987 3419 4166 4434 181
Size: 303 248 1320 432 747 268 909
Fragments arranged by size:
1320 909 747 432 379 303 268 248
142 130 85 79 79 24 17
EcoNI CCTnn'n_nnAGG
Cuts at: 203 388 454 710 1396 203
Size: 185 66 256 686 3969
Fragments arranged by size:
3969 686 256 185 66
EcoO109I rG'GnC_Cy
Cuts at: 634 651 743 824 825 900 966 2301 634
Size: 17 92 81 1 75 66 1335 3495
Fragments arranged by size:
3495 1335 92 81 75 66 17 1
EcoRI G'AATT_C
Cuts at: 1637 2166 1637
Size: 529 4633
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1845 3181 1845
Size: 1336 3826
FspI TGC'GCA
Cuts at: 331 2943 331
Size: 2612 2550
FspAI rTGC'GCAy
Cuts at: 331 331
Size: 5162
HaeII r_GCGC'y
Cuts at: 1147 2596 2844 3866 4845 1147
Size: 1449 248 1022 979 1464
Fragments arranged by size:
1464 1449 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 479 511 1590 1622 2011 2043 2109 2141 3004
Size: 32 1079 32 389 32 66 32 863
Cuts at: 3004 3034 3036 3066 479
Size: 30 2 30 2575
Fragments arranged by size:
2575 1079 863 389 66 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2201 2201
Size: 5162
HindIII A'AGCT_T
Cuts at: 1854 2106 1854
Size: 252 4910
KasI G'GCGC_C
Cuts at: 1143 2592 2840 1143
Size: 1449 248 3465
Fragments arranged by size:
3465 1449 248
KpnI G_GTAC'C
Cuts at: 2182 2182
Size: 5162
MluI A'CGCG_T
Cuts at: 4595 4595
Size: 5162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1556 1640 3833 4017 1556
Size: 84 2193 184 2701
Fragments arranged by size:
2701 2193 184 84
MscI TGG'CCA
Cuts at: 2923 2923
Size: 5162
MslI CAynn'nnrTG
Cuts at: 913 1478 2153 3151 3278 913
Size: 565 675 998 127 2797
Fragments arranged by size:
2797 998 675 565 127
MspA1I CmG'CkG
Cuts at: 265 1312 2273 2587 2947 3960 4205 265
Size: 1047 961 314 360 1013 245 1222
Fragments arranged by size:
1222 1047 1013 961 360 314 245
NaeI GCC'GGC
Cuts at: 289 3343 289
Size: 3054 2108
NarI GG'CG_CC
Cuts at: 1144 2593 2841 1144
Size: 1449 248 3465
Fragments arranged by size:
3465 1449 248
NcoI C'CATG_G
Cuts at: 107 306 504 1800 107
Size: 199 198 1296 3469
Fragments arranged by size:
3469 1296 199 198
NgoMIV G'CCGG_C
Cuts at: 287 3341 287
Size: 3054 2108
NspI r_CATG'y
Cuts at: 1309 1872 2215 2715 3622 1309
Size: 563 343 500 907 2849
Fragments arranged by size:
2849 907 563 500 343
PciI A'CATG_T
Cuts at: 3618 3618
Size: 5162
PflMI CCAn_nnn'nTGG
Cuts at: 1159 1159
Size: 5162
PfoI T'CCnGG_A
Cuts at: 990 990
Size: 5162
PmeI GTTT'AAAC
Cuts at: 2161 2161
Size: 5162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 4301
Size: 32 5130
PpuMI rG'GwC_Cy
Cuts at: 651 900 651
Size: 249 4913
PsiI TTA'TAA
Cuts at: 1661 1661
Size: 5162
PspOMI G'GGCC_C
Cuts at: 117 742 824 117
Size: 625 82 4455
Fragments arranged by size:
4455 625 82
PstI C_TGCA'G
Cuts at: 623 1226 2209 2894 623
Size: 603 983 685 2891
Fragments arranged by size:
2891 983 685 603
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5162
PvuII CAG'CTG
Cuts at: 265 1312 2587 2947 265
Size: 1047 1275 360 2480
Fragments arranged by size:
2480 1275 1047 360
RsrII CG'GwC_CG
Cuts at: 3357 3357
Size: 5162
SacI G_AGCT'C
Cuts at: 2176 2176
Size: 5162
SalI G'TCGA_C
Cuts at: 2199 2199
Size: 5162
SanDI GG'GwC_CC
Cuts at: 651 651
Size: 5162
SapI GCTCTTCn'nnn_
Cuts at: 3185 3395 3185
Size: 210 4952
SbfI CC_TGCA'GG
Cuts at: 2209 2209
Size: 5162
ScaI AGT'ACT
Cuts at: 4679 4679
Size: 5162
SfcI C'TryA_G
Cuts at: 33 619 1222 2205 2890 3883 4074 33
Size: 586 603 983 685 993 191 1121
Fragments arranged by size:
1121 993 983 685 603 586 191
SfoI GGC'GCC
Cuts at: 1145 2594 2842 1145
Size: 1449 248 3465
Fragments arranged by size:
3465 1449 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5162
SmaI CCC'GGG
Cuts at: 122 274 2184 122
Size: 152 1910 3100
Fragments arranged by size:
3100 1910 152
SmlI C'TyrA_G
Cuts at: 1188 2317 3232 3724 3986 1188
Size: 1129 915 492 262 2364
Fragments arranged by size:
2364 1129 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5162
SphI G_CATG'C
Cuts at: 1872 2215 2715 1872
Size: 343 500 4319
Fragments arranged by size:
4319 500 343
SrfI GCCC'GGGC
Cuts at: 122 122
Size: 5162
StuI AGG'CCT
Cuts at: 223 1174 223
Size: 951 4211
StyI C'CwwG_G
Cuts at: 107 306 504 1175 1800 2296 2650 107
Size: 199 198 671 625 496 354 2619
Fragments arranged by size:
2619 671 625 496 354 199 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 225 299 925 1227 1318 1462 1486 2745 2878
Size: 74 626 302 91 144 24 1259 133
Cuts at: 2878 3337 3546 225
Size: 459 209 1841
Fragments arranged by size:
1841 1259 626 459 302 209 144 133
91 74 24
TatI w'GTAC_w
Cuts at: 779 4677 779
Size: 3898 1264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 506 538 4679 4711 506
Size: 32 4141 32 957
Fragments arranged by size:
4141 957 32 32
Tth111I GACn'n_nGTC
Cuts at: 2959 2959
Size: 5162
XbaI T'CTAG_A
Cuts at: 2193 2193
Size: 5162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 262 2657 262
Size: 2395 2767
XmaI C'CCGG_G
Cuts at: 120 272 2182 120
Size: 152 1910 3100
Fragments arranged by size:
3100 1910 152
XmnI GAAnn'nnTTC
Cuts at: 1022 1432 1684 1855 1022
Size: 410 252 171 4329
Fragments arranged by size:
4329 410 252 171
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BfrBI BmtI BsiWI BsmI BsmBI BssHII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SspI SwaI XhoI ZraI