Restriction map for
pF1KB9559
(Circular) (Six-base) MAPSORT of: pF1KB9559.seq Check: 6707 from: 1 to: 6371
pF1KB9559 6371 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AatII G_ACGT'C
Cuts at: 1929 1929
Size: 6371
AccI GT'mk_AC
Cuts at: 2052 2322 3409 2052
Size: 270 1087 5014
Fragments arranged by size:
5014 1087 270
Acc65I G'GTAC_C
Cuts at: 2365 3387 2365
Size: 1022 5349
AclI AA'CG_TT
Cuts at: 1080 6211 1080
Size: 5131 1240
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 2731 4196 4628 4981 5643 580
Size: 2151 1465 432 353 662 1308
Fragments arranged by size:
2151 1465 1308 662 432 353
AfeI AGC'GCT
Cuts at: 1492 1492
Size: 6371
AflII C'TTAA_G
Cuts at: 153 153
Size: 6371
AflIII A'CryG_T
Cuts at: 526 1844 3041 3723 5523 5804 526
Size: 1318 1197 682 1800 281 1093
Fragments arranged by size:
1800 1318 1197 1093 682 281
AgeI A'CCGG_T
Cuts at: 289 289
Size: 6371
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2021 2053 2633 2665 2735 2767 2021
Size: 32 580 32 70 32 5625
Fragments arranged by size:
5625 580 70 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 967 2817 2931 3676 5114 967
Size: 1850 114 745 1438 2224
Fragments arranged by size:
2224 1850 1438 745 114
ApaI G_GGCC'C
Cuts at: 1549 1549
Size: 6371
ApaLI G'TGCA_C
Cuts at: 5209 5209
Size: 6371
ApoI r'AATT_y
Cuts at: 458 1177 2128 2264 3018 3136 3187 3375 5724
Size: 719 951 136 754 118 51 188 2349
Cuts at: 5724 5980 458
Size: 256 849
Fragments arranged by size:
2349 951 849 754 719 256 188 136
118 51
AseI AT'TA_AT
Cuts at: 20 191 833 20
Size: 171 642 5558
Fragments arranged by size:
5558 642 171
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6371
AvaI C'yCGr_G
Cuts at: 1765 3127 3391 1765
Size: 1362 264 4745
Fragments arranged by size:
4745 1362 264
BamHI G'GATC_C
Cuts at: 510 3396 510
Size: 2886 3485
BanI G'GyrC_C
Cuts at: 2365 2524 3387 3801 4049 4084 2365
Size: 159 863 414 248 35 4652
Fragments arranged by size:
4652 863 414 248 159 35
BanII G_rGCy'C
Cuts at: 799 1549 3385 4415 799
Size: 750 1836 1030 2755
Fragments arranged by size:
2755 1836 1030 750
BbeI G_GCGC'C
Cuts at: 3805 4053 3805
Size: 248 6123
BbsI GAAGACnn'nnnn_
Cuts at: 134 523 1961 3044 134
Size: 389 1438 1083 3461
Fragments arranged by size:
3461 1438 1083 389
BbvCI CC'TCA_GC
Cuts at: 1676 2978 1676
Size: 1302 5069
BciVI GTATCCnnnnn_n'
Cuts at: 3114 4269 5325 6371 3114
Size: 1155 1056 1046 3114
Fragments arranged by size:
3114 1155 1056 1046
BclI T'GATC_A
Cuts at: 2376 3891 2376
Size: 1515 4856
BfrBI ATG'CAT
Cuts at: 449 1920 449
Size: 1471 4900
BglI GCCn_nnn'nGGC
Cuts at: 1749 1749
Size: 6371
BglII A'GATC_T
Cuts at: 3153 3886 5923 3153
Size: 733 2037 3601
Fragments arranged by size:
3601 2037 733
BlpI GC'TnA_GC
Cuts at: 3483 3483
Size: 6371
Bme1580I G_kGCm'C
Cuts at: 1549 3996 4089 5213 1549
Size: 2447 93 1124 2707
Fragments arranged by size:
2707 2447 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2218 3583 3664 3999 6143 2218
Size: 1365 81 335 2144 2446
Fragments arranged by size:
2446 2144 1365 335 81
BmtI G_CTAG'C
Cuts at: 2303 2303
Size: 6371
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 478 4738 478
Size: 4260 2111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 640 1250 2090 2953 448
Size: 192 610 840 863 3866
Fragments arranged by size:
3866 863 840 610 192
Bpu10I CC'TnA_GC
Cuts at: 1232 1676 2978 1232
Size: 444 1302 4625
Fragments arranged by size:
4625 1302 444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1107 3547 4426 5140 5438 1107
Size: 2440 879 714 298 2040
Fragments arranged by size:
2440 2040 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 298 687 6075 298
Size: 389 5388 594
Fragments arranged by size:
5388 594 389
BsaAI yAC'GTr
Cuts at: 3724 3724
Size: 6371
BsaBI GATnn'nnATC
Cuts at: 6 3885 5922 6
Size: 3879 2037 455
Fragments arranged by size:
3879 2037 455
BsaHI Gr'CG_yC
Cuts at: 1926 2868 3802 4050 4752 6248 1926
Size: 942 934 248 702 1496 2049
Fragments arranged by size:
2049 1496 942 934 702 248
BsaWI w'CCGG_w
Cuts at: 289 3560 4081 4759 5170 5317 5591 289
Size: 3271 521 678 411 147 274 1069
Fragments arranged by size:
3271 1069 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 605 635 1626 1656 2021 2051 2063 2093 605
Size: 30 991 30 365 30 12 30 4883
Fragments arranged by size:
4883 991 365 30 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1634 2042 3248 1634
Size: 408 1206 4757
Fragments arranged by size:
4757 1206 408
BseYI C'CCAG_C
Cuts at: 361 1707 2316 2962 3796 4554 5219 5627 361
Size: 1346 609 646 834 758 665 408 1105
Fragments arranged by size:
1346 1105 834 758 665 646 609 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2459 2521 2459
Size: 62 6309
BsiEI CG_ry'CG
Cuts at: 104 3913 3959 5189 104
Size: 3809 46 1230 1286
Fragments arranged by size:
3809 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 434 799 928 2202 3385 4163 4353 5213 434
Size: 365 129 1274 1183 778 190 860 1592
Fragments arranged by size:
1592 1274 1183 860 778 365 190 129
BsmI GAATG_Cn'
Cuts at: 1787 1890 1787
Size: 103 6268
BsmBI CGTCTCn'nnnn_
Cuts at: 138 138
Size: 6371
Bsp1286I G_dGCh'C
Cuts at: 434 799 928 1549 2202 3385 3996 4089 4163
Size: 365 129 621 653 1183 611 93 74
Cuts at: 4163 4353 4415 5213 434
Size: 190 62 798 1592
Fragments arranged by size:
1592 1183 798 653 621 611 365 190
129 93 74 62
BspHI T'CATG_A
Cuts at: 4803 4803
Size: 6371
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3421 3635 3937 4318 3421
Size: 214 302 381 5474
Fragments arranged by size:
5474 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4663 4717 6201 6366 50
Size: 4613 54 1484 165 55
Fragments arranged by size:
4613 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 262 4283 262
Size: 4021 2350
BsrFI r'CCGG_y
Cuts at: 289 1973 4369 4550 289
Size: 1684 2396 181 2110
Fragments arranged by size:
2396 2110 1684 181
BssSI C'ACGA_G
Cuts at: 4642 5350 4642
Size: 708 5663
BstAPI GCAn_nnn'nTGC
Cuts at: 3748 3748
Size: 6371
BstBI TT'CG_AA
Cuts at: 4732 4732
Size: 6371
BstEII G'GTnAC_C
Cuts at: 319 319
Size: 6371
BstXI CCAn_nnnn'nTGG
Cuts at: 2316 3114 2316
Size: 798 5573
BstYI r'GATC_y
Cuts at: 1 335 510 888 1116 1254 2750 3153 3396
Size: 334 175 378 228 138 1496 403 243
Cuts at: 3396 3863 3886 4221 4467 4773 4785 4871 5923
Size: 467 23 335 246 306 12 86 1052
Cuts at: 5923 1
Size: 449
Fragments arranged by size:
1496 1052 467 449 403 378 335 334
306 246 243 228 175 138 86 23
12
BstZ17I GTA'TAC
Cuts at: 2323 2323
Size: 6371
Bsu36I CC'TnA_GG
Cuts at: 648 648
Size: 6371
BtgI C'CryG_G
Cuts at: 107 2732 2754 107
Size: 2625 22 3724
Fragments arranged by size:
3724 2625 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1419 4321 4502 4665 1419
Size: 2902 181 163 3125
Fragments arranged by size:
3125 2902 181 163
BtsI GCAGTG_nn'
Cuts at: 614 3614 614
Size: 3000 3371
ClaI AT'CG_AT
Cuts at: 2876 2876
Size: 6371
DraI TTT'AAA
Cuts at: 1077 3370 1077
Size: 2293 4078
DraIII CAC_nnn'GTG
Cuts at: 2800 2800
Size: 6371
DrdI GACnn_nn'nnGTC
Cuts at: 742 3166 4077 5421 742
Size: 2424 911 1344 1692
Fragments arranged by size:
2424 1692 1344 911
EaeI y'GGCC_r
Cuts at: 1389 3956 4130 4521 4548 5893 1389
Size: 2567 174 391 27 1345 1867
Fragments arranged by size:
2567 1867 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3956 3956
Size: 6371
EarI CTCTTCn'nnn_
Cuts at: 2111 2394 4394 4604 2111
Size: 283 2000 210 3878
Fragments arranged by size:
3878 2000 283 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2774 4579 5321 5467 6181 2774
Size: 1805 742 146 714 2964
Fragments arranged by size:
2964 1805 742 714 146
EcoICRI GAG'CTC
Cuts at: 797 3383 797
Size: 2586 3785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 580 640 1250 2090 2731 2953 4196 4628
Size: 132 60 610 840 641 222 1243 432
Cuts at: 4628 4981 5643 448
Size: 353 662 1176
Fragments arranged by size:
1243 1176 840 662 641 610 432 353
222 132 60
EcoO109I rG'GnC_Cy
Cuts at: 365 416 1545 2341 3510 365
Size: 51 1129 796 1169 3226
Fragments arranged by size:
3226 1169 1129 796 51
EcoRI G'AATT_C
Cuts at: 458 3375 458
Size: 2917 3454
EcoRV GAT'ATC
Cuts at: 1294 1294
Size: 6371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2790 4390 2790
Size: 1600 4771
FspI TGC'GCA
Cuts at: 4152 4152
Size: 6371
HaeII r_GCGC'y
Cuts at: 1494 3805 4053 5283 6054 1494
Size: 2311 248 1230 771 1811
Fragments arranged by size:
2311 1811 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 667 699 1659 1691 4213 4243 4245 4275 667
Size: 32 960 32 2522 30 2 30 2763
Fragments arranged by size:
2763 2522 960 32 32 30 30 2
HincII GTy'rAC
Cuts at: 260 2229 3410 260
Size: 1969 1181 3221
Fragments arranged by size:
3221 1969 1181
HindIII A'AGCT_T
Cuts at: 1694 1694
Size: 6371
KasI G'GCGC_C
Cuts at: 3801 4049 3801
Size: 248 6123
KpnI G_GTAC'C
Cuts at: 2369 3391 2369
Size: 1022 5349
MluI A'CGCG_T
Cuts at: 5804 5804
Size: 6371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 212 307 5130 5314 212
Size: 95 4823 184 1269
Fragments arranged by size:
4823 1269 184 95
MscI TGG'CCA
Cuts at: 4132 4132
Size: 6371
MslI CAynn'nnrTG
Cuts at: 4360 4487 4360
Size: 127 6244
MspA1I CmG'CkG
Cuts at: 1144 1327 1707 2790 3482 3796 4156 4940 5185
Size: 183 380 1083 692 314 360 784 245
Cuts at: 5185 1144
Size: 2330
Fragments arranged by size:
2330 1083 784 692 380 360 314 245
183
NaeI GCC'GGC
Cuts at: 4552 4552
Size: 6371
NarI GG'CG_CC
Cuts at: 3802 4050 3802
Size: 248 6123
NcoI C'CATG_G
Cuts at: 107 2732 107
Size: 2625 3746
NdeI CA'TA_TG
Cuts at: 1623 1623
Size: 6371
NgoMIV G'CCGG_C
Cuts at: 4550 4550
Size: 6371
NheI G'CTAG_C
Cuts at: 2299 2299
Size: 6371
NsiI A_TGCA'T
Cuts at: 451 1922 451
Size: 1471 4900
NspI r_CATG'y
Cuts at: 314 530 1848 2700 3284 3424 3924 5527 314
Size: 216 1318 852 584 140 500 1603 1158
Fragments arranged by size:
1603 1318 1158 852 584 500 216 140
PasI CC'CwG_GG
Cuts at: 3264 3264
Size: 6371
PciI A'CATG_T
Cuts at: 526 1844 5523 526
Size: 1318 3679 1374
Fragments arranged by size:
3679 1374 1318
PflMI CCAn_nnn'nTGG
Cuts at: 426 2733 426
Size: 2307 4064
PfoI T'CCnGG_A
Cuts at: 338 1949 338
Size: 1611 4760
PmeI GTTT'AAAC
Cuts at: 3370 3370
Size: 6371
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2021 2053 4817 4849 2021
Size: 32 2764 32 3543
Fragments arranged by size:
3543 2764 32 32
PpuMI rG'GwC_Cy
Cuts at: 416 416
Size: 6371
PshAI GACnn'nnGTC
Cuts at: 3353 3353
Size: 6371
PsiI TTA'TAA
Cuts at: 2597 2597
Size: 6371
PspOMI G'GGCC_C
Cuts at: 1545 1545
Size: 6371
PstI C_TGCA'G
Cuts at: 1895 2834 3418 4103 1895
Size: 939 584 685 4163
Fragments arranged by size:
4163 939 685 584
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6371
PvuII CAG'CTG
Cuts at: 1144 1327 1707 2790 3796 4156 1144
Size: 183 380 1083 1006 360 3359
Fragments arranged by size:
3359 1083 1006 380 360 183
RsrII CG'GwC_CG
Cuts at: 4566 4566
Size: 6371
SacI G_AGCT'C
Cuts at: 799 3385 799
Size: 2586 3785
SalI G'TCGA_C
Cuts at: 3408 3408
Size: 6371
SapI GCTCTTCn'nnn_
Cuts at: 2111 2394 4394 4604 2111
Size: 283 2000 210 3878
Fragments arranged by size:
3878 2000 283 210
SbfI CC_TGCA'GG
Cuts at: 3418 3418
Size: 6371
ScaI AGT'ACT
Cuts at: 1271 1312 5888 1271
Size: 41 4576 1754
Fragments arranged by size:
4576 1754 41
SfcI C'TryA_G
Cuts at: 33 1891 2053 2394 2830 3414 4099 5067 5258
Size: 1858 162 341 436 584 685 968 191
Cuts at: 5258 33
Size: 1146
Fragments arranged by size:
1858 1146 968 685 584 436 341 191
162
SfiI GGCCn_nnn'nGGCC
Cuts at: 1749 1749
Size: 6371
SfoI GGC'GCC
Cuts at: 3803 4051 3803
Size: 248 6123
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6371
SmaI CCC'GGG
Cuts at: 3393 3393
Size: 6371
SmlI C'TyrA_G
Cuts at: 153 1086 3526 4441 5155 5417 153
Size: 933 2440 915 714 262 1107
Fragments arranged by size:
2440 1107 933 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6371
SphI G_CATG'C
Cuts at: 2700 3424 3924 2700
Size: 724 500 5147
Fragments arranged by size:
5147 724 500
SspI AAT'ATT
Cuts at: 1909 3054 1909
Size: 1145 5226
StyI C'CwwG_G
Cuts at: 107 2732 2774 3505 3859 107
Size: 2625 42 731 354 2619
Fragments arranged by size:
2625 2619 731 354 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2272 2512 2623 2930 3954 4087 4546 4755 2272
Size: 240 111 307 1024 133 459 209 3888
Fragments arranged by size:
3888 1024 459 307 240 209 133 111
TatI w'GTAC_w
Cuts at: 1239 1269 1310 3068 3111 5886 1239
Size: 30 41 1758 43 2775 1724
Fragments arranged by size:
2775 1758 1724 43 41 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 605 637 1787 1819 5888 5920 605
Size: 32 1150 32 4069 32 1056
Fragments arranged by size:
4069 1150 1056 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4168 4168
Size: 6371
XbaI T'CTAG_A
Cuts at: 822 3156 3402 822
Size: 2334 246 3791
Fragments arranged by size:
3791 2334 246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3866 3866
Size: 6371
XmaI C'CCGG_G
Cuts at: 3391 3391
Size: 6371
XmnI GAAnn'nnTTC
Cuts at: 1661 2120 2267 1661
Size: 459 147 5765
Fragments arranged by size:
5765 459 147
ZraI GAC'GTC
Cuts at: 1927 1927
Size: 6371
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AhdI AleI AlfI AscI AvrII BaeI BcgI
BmgBI BsiWI BspEI BsrGI BssHII CspCI EcoNI FseI
FspAI HpaI MfeI NotI NruI PacI PmlI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI StuI
SwaI XhoI