Restriction map for
pF1KB9563
(Circular) (Six-base) MAPSORT of: pF1KB9563.seq Check: 7822 from: 1 to: 10079
pF1KB9563 10079 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 893 2248 2388 3414 3927 4284 6250 6511 893
Size: 1355 140 1026 513 357 1966 261 4461
Fragments arranged by size:
4461 1966 1355 1026 513 357 261 140
AatII G_ACGT'C
Cuts at: 6878 6878
Size: 10079
AccI GT'mk_AC
Cuts at: 3020 4637 5060 7117 3020
Size: 1617 423 2057 5982
Fragments arranged by size:
5982 2057 1617 423
Acc65I G'GTAC_C
Cuts at: 913 2333 7095 913
Size: 1420 4762 3897
Fragments arranged by size:
4762 3897 1420
AclI AA'CG_TT
Cuts at: 9919 9919
Size: 10079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 707 2089 3222 3844 4185 4879 6090 6162 7904
Size: 1382 1133 622 341 694 1211 72 1742
Cuts at: 7904 8336 8689 9351 707
Size: 432 353 662 1435
Fragments arranged by size:
1742 1435 1382 1211 1133 694 662 622
432 353 341 72
AfeI AGC'GCT
Cuts at: 5332 5332
Size: 10079
AflII C'TTAA_G
Cuts at: 7051 7051
Size: 10079
AflIII A'CryG_T
Cuts at: 6770 7431 9231 9512 6770
Size: 661 1800 281 7337
Fragments arranged by size:
7337 1800 661 281
AhdI GACnn_n'nnGTC
Cuts at: 3637 4620 3637
Size: 983 9096
AleI CACnn'nnGTG
Cuts at: 442 932 2000 2786 2974 3550 4696 5474 5507
Size: 490 1068 786 188 576 1146 778 33
Cuts at: 5507 6970 442
Size: 1463 3551
Fragments arranged by size:
3551 1463 1146 1068 786 778 576 490
188 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1260 2134 3791 4714 1260
Size: 874 1657 923 6625
Fragments arranged by size:
6625 1657 923 874
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2008 2040 3470 3502 2008
Size: 32 1430 32 8585
Fragments arranged by size:
8585 1430 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1401 1512 3400 3681 4299 4875 4945 4959 5701
Size: 111 1888 281 618 576 70 14 742
Cuts at: 5701 6885 7384 8822 1401
Size: 1184 499 1438 2658
Fragments arranged by size:
2658 1888 1438 1184 742 618 576 499
281 111 70 14
ApaI G_GGCC'C
Cuts at: 734 2226 3811 4262 4984 5670 5777 6510 6989
Size: 1492 1585 451 722 686 107 733 479
Cuts at: 6989 734
Size: 3824
Fragments arranged by size:
3824 1585 1492 733 722 686 479 451
107
ApaLI G'TGCA_C
Cuts at: 3388 3766 8917 3388
Size: 378 5151 4550
Fragments arranged by size:
5151 4550 378
ApoI r'AATT_y
Cuts at: 7083 9432 9688 7083
Size: 2349 256 7474
Fragments arranged by size:
7474 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10079
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10079
AvaI C'yCGr_G
Cuts at: 1078 1526 1549 1563 1685 3197 3252 4138 5098
Size: 448 23 14 122 1512 55 886 960
Cuts at: 5098 5156 5254 5639 5663 6401 6418 6643 7099
Size: 58 98 385 24 738 17 225 456
Cuts at: 7099 1078
Size: 4058
Fragments arranged by size:
4058 1512 960 886 738 456 448 385
225 122 98 58 55 24 23 17
14
BamHI G'GATC_C
Cuts at: 3413 7104 3413
Size: 3691 6388
BanI G'GyrC_C
Cuts at: 225 816 913 1689 1894 2333 2832 3535 3985
Size: 591 97 776 205 439 499 703 450
Cuts at: 3985 4683 5482 5730 5949 5961 6280 7095 7509
Size: 698 799 248 219 12 319 815 414
Cuts at: 7509 7757 7792 225
Size: 248 35 2512
Fragments arranged by size:
2512 815 799 776 703 698 591 499
450 439 414 319 248 248 219 205
97 35 12
BanII G_rGCy'C
Cuts at: 522 545 734 1550 1572 2226 3329 3811 3949
Size: 23 189 816 22 654 1103 482 138
Cuts at: 3949 4262 4323 4736 4984 5105 5135 5405 5670
Size: 313 61 413 248 121 30 270 265
Cuts at: 5670 5777 6243 6419 6510 6728 6989 7093 8123
Size: 107 466 176 91 218 261 104 1030
Cuts at: 8123 522
Size: 2478
Fragments arranged by size:
2478 1103 1030 816 654 482 466 413
313 270 265 261 248 218 189 176
138 121 107 104 91 61 30 23
22
BbeI G_GCGC'C
Cuts at: 3989 4687 7513 7761 3989
Size: 698 2826 248 6307
Fragments arranged by size:
6307 2826 698 248
BbsI GAAGACnn'nnnn_
Cuts at: 1181 3002 3117 3846 1181
Size: 1821 115 729 7414
Fragments arranged by size:
7414 1821 729 115
BbvCI CC'TCA_GC
Cuts at: 388 504 1045 3721 4285 4357 5606 5749 388
Size: 116 541 2676 564 72 1249 143 4718
Fragments arranged by size:
4718 2676 1249 564 541 143 116 72
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 10045
BciVI GTATCCnnnnn_n'
Cuts at: 7977 9033 10079 7977
Size: 1056 1046 7977
Fragments arranged by size:
7977 1056 1046
BclI T'GATC_A
Cuts at: 7599 7599
Size: 10079
BfrBI ATG'CAT
Cuts at: 6211 6211
Size: 10079
BglI GCCn_nnn'nGGC
Cuts at: 832 1318 1827 2293 2707 3333 3713 3907 5542
Size: 486 509 466 414 626 380 194 1635
Cuts at: 5542 5827 832
Size: 285 5084
Fragments arranged by size:
5084 1635 626 509 486 466 414 380
285 194
BglII A'GATC_T
Cuts at: 3845 7594 9631 3845
Size: 3749 2037 4293
Fragments arranged by size:
4293 3749 2037
BlpI GC'TnA_GC
Cuts at: 1490 5863 7191 1490
Size: 4373 1328 4378
Fragments arranged by size:
4378 4373 1328
Bme1580I G_kGCm'C
Cuts at: 559 734 766 1438 1536 1694 2226 2623 3392
Size: 175 32 672 98 158 532 397 769
Cuts at: 3392 3538 3770 3811 4262 4693 4984 5485 5514
Size: 146 232 41 451 431 291 501 29
Cuts at: 5514 5655 5670 5733 5777 5952 5966 6401 6510
Size: 141 15 63 44 175 14 435 109
Cuts at: 6510 6552 6989 7704 7797 8921 559
Size: 42 437 715 93 1124 1717
Fragments arranged by size:
1717 1124 769 715 672 532 501 451
437 435 431 397 291 232 175 175
158 146 141 109 98 93 63 44
42 41 32 29 15 14
BmgBI CAC'GTC
Cuts at: 278 1602 3231 3609 5163 278
Size: 1324 1629 378 1554 5194
Fragments arranged by size:
5194 1629 1554 1324 378
BmrI ACTGGGnnnn_n'
Cuts at: 569 2228 2428 6057 7077 7291 7372 7707 9851
Size: 1659 200 3629 1020 214 81 335 2144
Cuts at: 9851 569
Size: 797
Fragments arranged by size:
3629 2144 1659 1020 797 335 214 200
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1060 1665 8446 1060
Size: 605 6781 2693
Fragments arranged by size:
6781 2693 605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 386 1075 1096 1273 1687 2410 2494 2548
Size: 145 689 21 177 414 723 84 54
Cuts at: 2548 2560 3481 3489 3913 4110 4180 4321 4392
Size: 12 921 8 424 197 70 141 71
Cuts at: 4392 4917 5119 5503 5977 6385 6502 6559 6977
Size: 525 202 384 474 408 117 57 418
Cuts at: 6977 241
Size: 3343
Fragments arranged by size:
3343 921 723 689 525 474 424 418
414 408 384 202 197 177 145 141
117 84 71 70 57 54 21 12
8
Bpu10I CC'TnA_GC
Cuts at: 388 504 1045 1343 1358 2338 2416 3721 4285
Size: 116 541 298 15 980 78 1305 564
Cuts at: 4285 4357 5606 5749 6722 6751 388
Size: 72 1249 143 973 29 3716
Fragments arranged by size:
3716 1305 1249 980 973 564 541 298
143 116 78 72 29 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1468 3506 4328 6481 6781 7255 8134 8848 9146
Size: 2038 822 2153 300 474 879 714 298
Cuts at: 9146 1468
Size: 2401
Fragments arranged by size:
2401 2153 2038 879 822 714 474 300
298
BsaI GGTCTCn'nnnn_
Cuts at: 1919 2167 2500 3034 5248 6919 9783 1919
Size: 248 333 534 2214 1671 2864 2215
Fragments arranged by size:
2864 2215 2214 1671 534 333 248
BsaAI yAC'GTr
Cuts at: 2698 4404 7432 2698
Size: 1706 3028 5345
Fragments arranged by size:
5345 3028 1706
BsaBI GATnn'nnATC
Cuts at: 6 7593 9630 6
Size: 7587 2037 455
Fragments arranged by size:
7587 2037 455
BsaHI Gr'CG_yC
Cuts at: 351 3266 3986 4145 4684 6557 6875 7510 7758
Size: 2915 720 159 539 1873 318 635 248
Cuts at: 7758 8460 9956 351
Size: 702 1496 474
Fragments arranged by size:
2915 1873 1496 720 702 635 539 474
318 248 159
BsaWI w'CCGG_w
Cuts at: 7268 7789 8467 8878 9025 9299 7268
Size: 521 678 411 147 274 8048
Fragments arranged by size:
8048 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1249 1279 3470 3500 4057 4087 5698 5728 6145
Size: 30 2191 30 557 30 1611 30 417
Cuts at: 6145 6175 6794 6824 1249
Size: 30 619 30 4504
Fragments arranged by size:
4504 2191 1611 619 557 417 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 397 492 637 1052 1270 1273 1276 1890 2557
Size: 95 145 415 218 3 3 614 667
Cuts at: 2557 2815 3124 3286 4614 4840 5413 5973 6040
Size: 258 309 162 1328 226 573 560 67
Cuts at: 6040 6150 6153 397
Size: 110 3 4323
Fragments arranged by size:
4323 1328 667 614 573 560 415 309
258 226 218 162 145 110 95 67
3 3 3
BseYI C'CCAG_C
Cuts at: 281 315 464 1161 1394 1824 2498 3269 3626
Size: 34 149 697 233 430 674 771 357
Cuts at: 3626 3671 4006 4310 4654 4718 4952 4985 5009
Size: 45 335 304 344 64 234 33 24
Cuts at: 5009 5065 5210 5218 5327 5371 5413 5438 5486
Size: 56 145 8 109 44 42 25 48
Cuts at: 5486 5669 6474 7504 8262 8927 9335 281
Size: 183 805 1030 758 665 408 1025
Fragments arranged by size:
1030 1025 805 771 758 697 674 665
430 408 357 344 335 304 234 233
183 149 145 109 64 56 48 45
44 42 34 33 25 24 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1483 1994 2416 2467 3187 3487 4042 4054 5959
Size: 511 422 51 720 300 555 12 1905
Cuts at: 5959 1483
Size: 5603
Fragments arranged by size:
5603 1905 720 555 511 422 300 51
12
BsiEI CG_ry'CG
Cuts at: 104 1949 2089 3551 7621 7667 8897 104
Size: 1845 140 1462 4070 46 1230 1286
Fragments arranged by size:
4070 1845 1462 1286 1230 140 46
BsiHKAI G_wGCw'C
Cuts at: 1550 2344 3392 3557 3770 4134 4606 4802 4850
Size: 794 1048 165 213 364 472 196 48
Cuts at: 4850 5135 5351 7093 7871 8061 8921 1550
Size: 285 216 1742 778 190 860 2708
Fragments arranged by size:
2708 1742 1048 860 794 778 472 364
285 216 213 196 190 165 48
BsmBI CGTCTCn'nnnn_
Cuts at: 403 1191 403
Size: 788 9291
Bsp1286I G_dGCh'C
Cuts at: 522 545 559 734 766 1438 1536 1550 1572
Size: 23 14 175 32 672 98 14 22
Cuts at: 1572 1694 2226 2344 2623 3329 3392 3538 3557
Size: 122 532 118 279 706 63 146 19
Cuts at: 3557 3770 3811 3949 4134 4262 4323 4606 4693
Size: 213 41 138 185 128 61 283 87
Cuts at: 4693 4736 4802 4850 4984 5105 5135 5351 5405
Size: 43 66 48 134 121 30 216 54
Cuts at: 5405 5485 5514 5655 5670 5733 5777 5952 5966
Size: 80 29 141 15 63 44 175 14
Cuts at: 5966 6243 6401 6419 6510 6552 6728 6989 7093
Size: 277 158 18 91 42 176 261 104
Cuts at: 7093 7704 7797 7871 8061 8123 8921 522
Size: 611 93 74 190 62 798 1680
Fragments arranged by size:
1680 798 706 672 611 532 283 279
277 261 216 213 190 185 176 175
175 158 146 141 138 134 128 122
121 118 104 98 93 91 87 80
74 66 63 63 62 61 54 48
44 43 42 41 32 30 29 23
22 19 18 15 14 14 14
BspHI T'CATG_A
Cuts at: 6168 8511 6168
Size: 2343 7736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 266 348 893 2248 2388 3414 3689 3927 4076
Size: 82 545 1355 140 1026 275 238 149
Cuts at: 4076 4284 6250 6511 7129 7343 7645 8026 266
Size: 208 1966 261 618 214 302 381 2319
Fragments arranged by size:
2319 1966 1355 1026 618 545 381 302
275 261 238 214 208 149 140 82
BsrBI CCG'CTC
Cuts at: 50 2922 5598 6963 8371 8425 9909 10074 50
Size: 2872 2676 1365 1408 54 1484 165 55
Fragments arranged by size:
2872 2676 1484 1408 1365 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3794 7991 3794
Size: 4197 5882
BsrFI r'CCGG_y
Cuts at: 809 842 989 1240 1941 1975 2088 3011 3958
Size: 33 147 251 701 34 113 923 947
Cuts at: 3958 4994 8077 8258 809
Size: 1036 3083 181 2630
Fragments arranged by size:
3083 2630 1036 947 923 701 251 181
147 113 34 33
BssSI C'ACGA_G
Cuts at: 2827 4735 5648 8350 9058 2827
Size: 1908 913 2702 708 3848
Fragments arranged by size:
3848 2702 1908 913 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3681 5091 5346 7456 3681
Size: 1410 255 2110 6304
Fragments arranged by size:
6304 2110 1410 255
BstBI TT'CG_AA
Cuts at: 1030 8440 1030
Size: 7410 2669
BstEII G'GTnAC_C
Cuts at: 2676 2791 2676
Size: 115 9964
BstXI CCAn_nnnn'nTGG
Cuts at: 1891 2002 2184 2788 3513 4278 5307 5476 5494
Size: 111 182 604 725 765 1029 169 18
Cuts at: 5494 5904 6798 1891
Size: 410 894 5172
Fragments arranged by size:
5172 1029 894 765 725 604 410 182
169 111 18
BstYI r'GATC_y
Cuts at: 1 2099 2411 3182 3413 3845 4629 7104 7571
Size: 2098 312 771 231 432 784 2475 467
Cuts at: 7571 7594 7929 8175 8481 8493 8579 9631 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
2475 2098 1052 784 771 467 449 432
335 312 306 246 231 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 583 604 1316 1832 2837 3364 4082 4119 4211
Size: 21 712 516 1005 527 718 37 92
Cuts at: 4211 4921 583
Size: 710 5741
Fragments arranged by size:
5741 1005 718 712 710 527 516 92
37 21
BtgI C'CryG_G
Cuts at: 121 191 236 893 1004 1060 1331 1539 1913
Size: 70 45 657 111 56 271 208 374
Cuts at: 1913 1998 2183 2372 2931 3405 3737 3749 5279
Size: 85 185 189 559 474 332 12 1530
Cuts at: 5279 5469 5540 6268 6707 121
Size: 190 71 728 439 3493
Fragments arranged by size:
3493 1530 728 657 559 474 439 374
332 271 208 190 189 185 111 85
71 70 56 45 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2051 3257 4109 8029 8210 8373 2051
Size: 1206 852 3920 181 163 3757
Fragments arranged by size:
3920 3757 1206 852 181 163
BtsI GCAGTG_nn'
Cuts at: 2479 3096 3449 3591 3917 4049 4240 4571 6348
Size: 617 353 142 326 132 191 331 1777
Cuts at: 6348 7322 2479
Size: 974 5236
Fragments arranged by size:
5236 1777 974 617 353 331 326 191
142 132
DraI TTT'AAA
Cuts at: 7078 7078
Size: 10079
DraIII CAC_nnn'GTG
Cuts at: 441 2459 3955 441
Size: 2018 1496 6565
Fragments arranged by size:
6565 2018 1496
DrdI GACnn_nn'nnGTC
Cuts at: 1183 4438 6304 6880 7785 9129 1183
Size: 3255 1866 576 905 1344 2133
Fragments arranged by size:
3255 2133 1866 1344 905 576
EaeI y'GGCC_r
Cuts at: 176 233 636 944 1191 1324 1424 2060 2078
Size: 57 403 308 247 133 100 636 18
Cuts at: 2078 2086 2090 2637 2670 3009 3134 3314 3494
Size: 8 4 547 33 339 125 180 180
Cuts at: 3494 3548 5165 5309 5828 6326 6381 6734 6978
Size: 54 1617 144 519 498 55 353 244
Cuts at: 6978 7059 7664 7838 8229 8256 9601 176
Size: 81 605 174 391 27 1345 654
Fragments arranged by size:
1617 1345 654 636 605 547 519 498
403 391 353 339 308 247 244 180
180 174 144 133 125 100 81 57
55 54 33 27 18 8 4
EagI C'GGCC_G
Cuts at: 2086 3548 7664 2086
Size: 1462 4116 4501
Fragments arranged by size:
4501 4116 1462
EarI CTCTTCn'nnn_
Cuts at: 683 3364 3937 4379 5356 6841 8102 8312 683
Size: 2681 573 442 977 1485 1261 210 2450
Fragments arranged by size:
2681 2450 1485 1261 977 573 442 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4771 5916 6346 8287 9029 9175 9889 4771
Size: 1145 430 1941 742 146 714 4961
Fragments arranged by size:
4961 1941 1145 742 714 430 146
EcoICRI GAG'CTC
Cuts at: 1548 5133 7091 1548
Size: 3585 1958 4536
Fragments arranged by size:
4536 3585 1958
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 386 707 1075 1096 1273 1687 2089 2410
Size: 145 321 368 21 177 414 402 321
Cuts at: 2410 2494 2548 2560 3222 3481 3489 3844 3913
Size: 84 54 12 662 259 8 355 69
Cuts at: 3913 4110 4180 4185 4321 4392 4879 4917 5119
Size: 197 70 5 136 71 487 38 202
Cuts at: 5119 5503 5977 6090 6162 6385 6502 6559 6977
Size: 384 474 113 72 223 117 57 418
Cuts at: 6977 7904 8336 8689 9351 241
Size: 927 432 353 662 969
Fragments arranged by size:
969 927 662 662 487 474 432 418
414 402 384 368 355 353 321 321
259 223 202 197 177 145 136 117
113 84 72 71 70 69 57 54
38 21 12 8 5
EcoNI CCTnn'n_nnAGG
Cuts at: 582 1361 1483 2542 2908 3524 4327 6052 6364
Size: 779 122 1059 366 616 803 1725 312
Cuts at: 6364 6995 582
Size: 631 3666
Fragments arranged by size:
3666 1725 1059 803 779 631 616 366
312 122
EcoO109I rG'GnC_Cy
Cuts at: 531 563 731 1828 1872 1887 2222 2223 3291
Size: 32 168 1097 44 15 335 1 1068
Cuts at: 3291 3360 3678 3807 4059 4259 4352 4980 4981
Size: 69 318 129 252 200 93 628 1
Cuts at: 4981 5031 5250 5534 5773 6058 6370 6456 6506
Size: 50 219 284 239 285 312 86 50
Cuts at: 6506 6507 6703 6882 6985 6986 7218 531
Size: 1 196 179 103 1 232 3392
Fragments arranged by size:
3392 1097 1068 628 335 318 312 285
284 252 239 232 219 200 196 179
168 129 103 93 86 69 50 50
44 32 15 1 1 1 1
EcoRI G'AATT_C
Cuts at: 7083 7083
Size: 10079
EcoRV GAT'ATC
Cuts at: 3057 3057
Size: 10079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 313 1638 2623 6762 8098 313
Size: 1325 985 4139 1336 2294
Fragments arranged by size:
4139 2294 1336 1325 985
FseI GG_CCGG'CC
Cuts at: 2092 2092
Size: 10079
FspI TGC'GCA
Cuts at: 1498 4702 7860 1498
Size: 3204 3158 3717
Fragments arranged by size:
3717 3204 3158
HaeII r_GCGC'y
Cuts at: 1923 3989 4364 4687 5217 5334 5982 6820 7513
Size: 2066 375 323 530 117 648 838 693
Cuts at: 7513 7761 8991 9762 1923
Size: 248 1230 771 2240
Fragments arranged by size:
2240 2066 1230 838 771 693 648 530
375 323 248 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1843 1875 3122 3154 3235 3267 3623 3655 4087
Size: 32 1247 32 81 32 356 32 432
Cuts at: 4087 4102 4119 4134 4600 4606 4632 4638 5677
Size: 15 17 15 466 6 26 6 1039
Cuts at: 5677 5709 7921 7951 7953 7983 1843
Size: 32 2212 30 2 30 3939
Fragments arranged by size:
3939 2212 1247 1039 466 432 356 81
32 32 32 32 32 30 30 26
17 15 15 6 6 2
HincII GTy'rAC
Cuts at: 1746 7118 1746
Size: 5372 4707
HindIII A'AGCT_T
Cuts at: 1214 1295 5969 1214
Size: 81 4674 5324
Fragments arranged by size:
5324 4674 81
KasI G'GCGC_C
Cuts at: 3985 4683 7509 7757 3985
Size: 698 2826 248 6307
Fragments arranged by size:
6307 2826 698 248
KpnI G_GTAC'C
Cuts at: 917 2337 7099 917
Size: 1420 4762 3897
Fragments arranged by size:
4762 3897 1420
MluI A'CGCG_T
Cuts at: 9512 9512
Size: 10079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1676 1949 3455 8838 9022 1676
Size: 273 1506 5383 184 2733
Fragments arranged by size:
5383 2733 1506 273 184
MscI TGG'CCA
Cuts at: 178 235 1193 1326 2080 2639 3136 3496 5311
Size: 57 958 133 754 559 497 360 1815
Cuts at: 5311 5830 6328 6736 6980 7061 7840 178
Size: 519 498 408 244 81 779 2417
Fragments arranged by size:
2417 1815 958 779 754 559 519 498
497 408 360 244 133 81 57
MslI CAynn'nnrTG
Cuts at: 442 862 932 980 2000 2786 2974 3550 3773
Size: 420 70 48 1020 786 188 576 223
Cuts at: 3773 4696 5474 5507 5578 5935 6790 6970 8068
Size: 923 778 33 71 357 855 180 1098
Cuts at: 8068 8195 442
Size: 127 2326
Fragments arranged by size:
2326 1098 1020 923 855 786 778 576
420 357 223 188 180 127 71 70
48 33
MspA1I CmG'CkG
Cuts at: 123 193 264 319 1398 1659 1824 2000 2307
Size: 70 71 55 1079 261 165 176 307
Cuts at: 2307 3273 3420 3616 3871 3891 4174 4392 4722
Size: 966 147 196 255 20 283 218 330
Cuts at: 4722 5139 5265 5385 5442 5673 5727 5862 5943
Size: 417 126 120 57 231 54 135 81
Cuts at: 5943 6432 6730 6970 7190 7504 7864 8648 8893
Size: 489 298 240 220 314 360 784 245
Cuts at: 8893 123
Size: 1309
Fragments arranged by size:
1309 1079 966 784 489 417 360 330
314 307 298 283 261 255 245 240
231 220 218 196 176 165 147 135
126 120 81 71 70 57 55 54
20
NaeI GCC'GGC
Cuts at: 811 844 1242 1943 1977 2090 3013 3960 4996
Size: 33 398 701 34 113 923 947 1036
Cuts at: 4996 8260 811
Size: 3264 2630
Fragments arranged by size:
3264 2630 1036 947 923 701 398 113
34 33
NarI GG'CG_CC
Cuts at: 3986 4684 7510 7758 3986
Size: 698 2826 248 6307
Fragments arranged by size:
6307 2826 698 248
NcoI C'CATG_G
Cuts at: 1331 1539 2931 3737 6268 6707 1331
Size: 208 1392 806 2531 439 4703
Fragments arranged by size:
4703 2531 1392 806 439 208
NgoMIV G'CCGG_C
Cuts at: 809 842 1240 1941 1975 2088 3011 3958 4994
Size: 33 398 701 34 113 923 947 1036
Cuts at: 4994 8258 809
Size: 3264 2630
Fragments arranged by size:
3264 2630 1036 947 923 701 398 113
34 33
NsiI A_TGCA'T
Cuts at: 6213 6213
Size: 10079
NspI r_CATG'y
Cuts at: 1462 5871 6253 6774 7132 7632 9235 1462
Size: 4409 382 521 358 500 1603 2306
Fragments arranged by size:
4409 2306 1603 521 500 382 358
PasI CC'CwG_GG
Cuts at: 717 4888 6276 717
Size: 4171 1388 4520
Fragments arranged by size:
4520 4171 1388
PciI A'CATG_T
Cuts at: 6770 9231 6770
Size: 2461 7618
PflMI CCAn_nnn'nTGG
Cuts at: 441 741 2459 4485 5388 5973 6435 441
Size: 300 1718 2026 903 585 462 4085
Fragments arranged by size:
4085 2026 1718 903 585 462 300
PfoI T'CCnGG_A
Cuts at: 1112 1595 1802 4369 6362 6993 1112
Size: 483 207 2567 1993 631 4198
Fragments arranged by size:
4198 2567 1993 631 483 207
PmeI GTTT'AAAC
Cuts at: 7078 7078
Size: 10079
PmlI CAC'GTG
Cuts at: 2698 2698
Size: 10079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 487 519 3470 3502 8525 8557 487
Size: 32 2951 32 5023 32 2009
Fragments arranged by size:
5023 2951 2009 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 531 563 1887 3291 3360 3678 4059 4352 5250
Size: 32 1324 1404 69 318 381 293 898
Cuts at: 5250 6058 6370 6456 6882 531
Size: 808 312 86 426 3728
Fragments arranged by size:
3728 1404 1324 898 808 426 381 318
312 293 86 69 32
PspOMI G'GGCC_C
Cuts at: 730 2222 3807 4258 4980 5666 5773 6506 6985
Size: 1492 1585 451 722 686 107 733 479
Cuts at: 6985 730
Size: 3824
Fragments arranged by size:
3824 1585 1492 733 722 686 479 451
107
PspXI vC'TCGA_Gb
Cuts at: 1549 4138 1549
Size: 2589 7490
PstI C_TGCA'G
Cuts at: 263 269 338 359 398 1249 2048 2231 2246
Size: 6 69 21 39 851 799 183 15
Cuts at: 2246 3425 3924 4295 4328 4556 5435 5451 7025
Size: 1179 499 371 33 228 879 16 1574
Cuts at: 7025 7126 7811 263
Size: 101 685 2531
Fragments arranged by size:
2531 1574 1179 879 851 799 685 499
371 228 183 101 69 39 33 21
16 15 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10079
PvuII CAG'CTG
Cuts at: 264 319 1398 1659 3273 3420 3616 3871 3891
Size: 55 1079 261 1614 147 196 255 20
Cuts at: 3891 4392 4722 5139 5385 5442 5673 5862 5943
Size: 501 330 417 246 57 231 189 81
Cuts at: 5943 6432 6970 7504 7864 264
Size: 489 538 534 360 2479
Fragments arranged by size:
2479 1614 1079 538 534 501 489 417
360 330 261 255 246 231 196 189
147 81 57 55 20
RsrII CG'GwC_CG
Cuts at: 8274 8274
Size: 10079
SacI G_AGCT'C
Cuts at: 1550 5135 7093 1550
Size: 3585 1958 4536
Fragments arranged by size:
4536 3585 1958
SacII CC_GC'GG
Cuts at: 124 194 2001 124
Size: 70 1807 8202
Fragments arranged by size:
8202 1807 70
SalI G'TCGA_C
Cuts at: 7116 7116
Size: 10079
SanDI GG'GwC_CC
Cuts at: 563 5250 6058 6370 563
Size: 4687 808 312 4272
Fragments arranged by size:
4687 4272 808 312
SapI GCTCTTCn'nnn_
Cuts at: 3937 4379 5356 6841 8102 8312 3937
Size: 442 977 1485 1261 210 5704
Fragments arranged by size:
5704 1485 1261 977 442 210
SbfI CC_TGCA'GG
Cuts at: 359 4295 7126 359
Size: 3936 2831 3312
Fragments arranged by size:
3936 3312 2831
ScaI AGT'ACT
Cuts at: 3380 7066 9596 3380
Size: 3686 2530 3863
Fragments arranged by size:
3863 3686 2530
SexAI A'CCwGG_T
Cuts at: 3965 3965
Size: 10079
SfcI C'TryA_G
Cuts at: 33 259 265 334 355 394 1245 2044 2227
Size: 226 6 69 21 39 851 799 183
Cuts at: 2227 2242 2614 3221 3421 3702 3920 4291 4324
Size: 15 372 607 200 281 218 371 33
Cuts at: 4324 4552 5431 5447 6618 7021 7122 7807 8775
Size: 228 879 16 1171 403 101 685 968
Cuts at: 8775 8966 33
Size: 191 1146
Fragments arranged by size:
1171 1146 968 879 851 799 685 607
403 372 371 281 228 226 218 200
191 183 101 69 39 33 21 16
15 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 5827 5827
Size: 10079
SfoI GGC'GCC
Cuts at: 3987 4685 7511 7759 3987
Size: 698 2826 248 6307
Fragments arranged by size:
6307 2826 698 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10079
SmaI CCC'GGG
Cuts at: 1565 3199 5158 5665 6420 7101 1565
Size: 1634 1959 507 755 681 4543
Fragments arranged by size:
4543 1959 1634 755 681 507
SmlI C'TyrA_G
Cuts at: 1447 1549 3521 4138 4343 6460 6760 7051 7234
Size: 102 1972 617 205 2117 300 291 183
Cuts at: 7234 8149 8863 9125 1447
Size: 915 714 262 2401
Fragments arranged by size:
2401 2117 1972 915 714 617 300 291
262 205 183 102
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10079
SphI G_CATG'C
Cuts at: 7132 7632 7132
Size: 500 9579
SspI AAT'ATT
Cuts at: 5073 5073
Size: 10079
StuI AGG'CCT
Cuts at: 1099 1846 3472 4087 4124 4550 5566 5617 1099
Size: 747 1626 615 37 426 1016 51 5561
Fragments arranged by size:
5561 1626 1016 747 615 426 51 37
StyI C'CwwG_G
Cuts at: 152 567 821 1331 1539 1890 2130 2931 3737
Size: 415 254 510 208 351 240 801 806
Cuts at: 3737 3989 6268 6707 6737 7213 7567 152
Size: 252 2279 439 30 476 354 2664
Fragments arranged by size:
2664 2279 806 801 510 476 439 415
354 351 254 252 240 208 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 262 1408 1963 2512 2665 3367 4020 4117 5500
Size: 1146 555 549 153 702 653 97 1383
Cuts at: 5500 5749 7053 7059 7662 7795 8254 8463 262
Size: 249 1304 6 603 133 459 209 1878
Fragments arranged by size:
1878 1383 1304 1146 702 653 603 555
549 459 249 209 153 133 97 6
TatI w'GTAC_w
Cuts at: 3378 7064 9594 3378
Size: 3686 2530 3863
Fragments arranged by size:
3863 3686 2530
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2282 2314 6542 6574 9596 9628 2282
Size: 32 4228 32 3022 32 2733
Fragments arranged by size:
4228 3022 2733 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7876 7876
Size: 10079
XbaI T'CTAG_A
Cuts at: 7110 7110
Size: 10079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 708 2070 2166 2523 3456 3613 5178 5277 6291
Size: 1362 96 357 933 157 1565 99 1014
Cuts at: 6291 7574 708
Size: 1283 3213
Fragments arranged by size:
3213 1565 1362 1283 1014 933 357 157
99 96
XhoI C'TCGA_G
Cuts at: 1549 4138 1549
Size: 2589 7490
XmaI C'CCGG_G
Cuts at: 1563 3197 5156 5663 6418 7099 1563
Size: 1634 1959 507 755 681 4543
Fragments arranged by size:
4543 1959 1634 755 681 507
ZraI GAC'GTC
Cuts at: 6876 6876
Size: 10079
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AgeI AscI AvrII BaeI BmtI BsiWI BsmI BspEI
BsrGI BssHII BstZ17I ClaI CspCI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PshAI PsiI PsrI
SgrAI SnaBI SrfI SwaI XmnI