Restriction map for
pF1KB9564
(Circular) (Six-base) MAPSORT of: pF1KB9564.seq Check: 594 from: 1 to: 3842
pF1KB9564 3842 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 530 880 530
Size: 350 3492
Acc65I G'GTAC_C
Cuts at: 858 858
Size: 3842
AclI AA'CG_TT
Cuts at: 3682 3682
Size: 3842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 616 1667 2099 2452 3114 490
Size: 126 1051 432 353 662 1218
Fragments arranged by size:
1218 1051 662 432 353 126
AflIII A'CryG_T
Cuts at: 1194 2994 3275 1194
Size: 1800 281 1761
Fragments arranged by size:
1800 1761 281
AlwNI CAG_nnn'CTG
Cuts at: 426 468 1147 2585 426
Size: 42 679 1438 1683
Fragments arranged by size:
1683 1438 679 42
ApaI G_GGCC'C
Cuts at: 522 666 738 819 522
Size: 144 72 81 3545
Fragments arranged by size:
3545 144 81 72
ApaLI G'TGCA_C
Cuts at: 2680 2680
Size: 3842
ApoI r'AATT_y
Cuts at: 846 3195 3451 846
Size: 2349 256 1237
Fragments arranged by size:
2349 1237 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3842
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
AvaI C'yCGr_G
Cuts at: 659 722 742 862 659
Size: 63 20 120 3639
Fragments arranged by size:
3639 120 63 20
BamHI G'GATC_C
Cuts at: 581 867 581
Size: 286 3556
BanI G'GyrC_C
Cuts at: 642 791 858 1272 1520 1555 642
Size: 149 67 414 248 35 2929
Fragments arranged by size:
2929 414 248 149 67 35
BanII G_rGCy'C
Cuts at: 522 666 738 819 856 1886 522
Size: 144 72 81 37 1030 2478
Fragments arranged by size:
2478 1030 144 81 72 37
BbeI G_GCGC'C
Cuts at: 795 1276 1524 795
Size: 481 248 3113
Fragments arranged by size:
3113 481 248
BbsI GAAGACnn'nnnn_
Cuts at: 338 338
Size: 3842
BciVI GTATCCnnnnn_n'
Cuts at: 1740 2796 3842 1740
Size: 1056 1046 1740
Fragments arranged by size:
1740 1056 1046
BclI T'GATC_A
Cuts at: 1362 1362
Size: 3842
BglI GCCn_nnn'nGGC
Cuts at: 733 814 733
Size: 81 3761
BglII A'GATC_T
Cuts at: 1357 3394 1357
Size: 2037 1805
BlpI GC'TnA_GC
Cuts at: 475 703 954 475
Size: 228 251 3363
Fragments arranged by size:
3363 251 228
Bme1580I G_kGCm'C
Cuts at: 522 666 738 819 1467 1560 2684 522
Size: 144 72 81 648 93 1124 1680
Fragments arranged by size:
1680 1124 648 144 93 81 72
BmrI ACTGGGnnnn_n'
Cuts at: 201 250 1054 1135 1470 3614 201
Size: 49 804 81 335 2144 429
Fragments arranged by size:
2144 804 429 335 81 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 3842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 102
Size: 3842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1897 2611 2909 1018
Size: 879 714 298 1951
Fragments arranged by size:
1951 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 316 3546 316
Size: 3230 612
BsaAI yAC'GTr
Cuts at: 1195 1195
Size: 3842
BsaBI GATnn'nnATC
Cuts at: 6 1356 3393 6
Size: 1350 2037 455
Fragments arranged by size:
2037 1350 455
BsaHI Gr'CG_yC
Cuts at: 792 1273 1521 2223 3719 792
Size: 481 248 702 1496 915
Fragments arranged by size:
1496 915 702 481 248
BsaWI w'CCGG_w
Cuts at: 1031 1552 2230 2641 2788 3062 1031
Size: 521 678 411 147 274 1811
Fragments arranged by size:
1811 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 541 793 844 847 178
Size: 363 252 51 3 3173
Fragments arranged by size:
3173 363 252 51 3
BseYI C'CCAG_C
Cuts at: 573 1267 2025 2690 3098 573
Size: 694 758 665 408 1317
Fragments arranged by size:
1317 758 694 665 408
BsiEI CG_ry'CG
Cuts at: 104 1384 1430 2660 104
Size: 1280 46 1230 1286
Fragments arranged by size:
1286 1280 1230 46
BsiHKAI G_wGCw'C
Cuts at: 856 1634 1824 2684 856
Size: 778 190 860 2014
Fragments arranged by size:
2014 860 778 190
BsmI GAATG_Cn'
Cuts at: 435 435
Size: 3842
Bsp1286I G_dGCh'C
Cuts at: 522 666 738 819 856 1467 1560 1634 1824
Size: 144 72 81 37 611 93 74 190
Cuts at: 1824 1886 2684 522
Size: 62 798 1680
Fragments arranged by size:
1680 798 611 190 144 93 81 74
72 62 37
BspHI T'CATG_A
Cuts at: 2274 2274
Size: 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 892 1106 1408 1789 892
Size: 214 302 381 2945
Fragments arranged by size:
2945 381 302 214
BsrBI CCG'CTC
Cuts at: 50 805 2134 2188 3672 3837 50
Size: 755 1329 54 1484 165 55
Fragments arranged by size:
1484 1329 755 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1754 1754
Size: 3842
BsrFI r'CCGG_y
Cuts at: 616 707 1840 2021 616
Size: 91 1133 181 2437
Fragments arranged by size:
2437 1133 181 91
BssSI C'ACGA_G
Cuts at: 2113 2821 2113
Size: 708 3134
BstAPI GCAn_nnn'nTGC
Cuts at: 1219 1219
Size: 3842
BstBI TT'CG_AA
Cuts at: 2203 2203
Size: 3842
BstXI CCAn_nnnn'nTGG
Cuts at: 165 333 387 165
Size: 168 54 3620
Fragments arranged by size:
3620 168 54
BstYI r'GATC_y
Cuts at: 1 581 867 1334 1357 1692 1938 2244 2256
Size: 580 286 467 23 335 246 306 12
Cuts at: 2256 2342 3394 1
Size: 86 1052 449
Fragments arranged by size:
1052 580 467 449 335 306 286 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 133 107
Size: 26 3816
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1792 1973 2136 1792
Size: 181 163 3498
Fragments arranged by size:
3498 181 163
BtsI GCAGTG_nn'
Cuts at: 828 1085 828
Size: 257 3585
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 343 378 343
Size: 35 3807
DraI TTT'AAA
Cuts at: 841 841
Size: 3842
DraIII CAC_nnn'GTG
Cuts at: 336 336
Size: 3842
DrdI GACnn_nn'nnGTC
Cuts at: 1548 2892 1548
Size: 1344 2498
EaeI y'GGCC_r
Cuts at: 483 614 806 1427 1601 1992 2019 3364 483
Size: 131 192 621 174 391 27 1345 961
Fragments arranged by size:
1345 961 621 391 192 174 131 27
EagI C'GGCC_G
Cuts at: 1427 1427
Size: 3842
EarI CTCTTCn'nnn_
Cuts at: 238 1865 2075 238
Size: 1627 210 2005
Fragments arranged by size:
2005 1627 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 594 2050 2792 2938 3652 594
Size: 1456 742 146 714 784
Fragments arranged by size:
1456 784 742 714 146
EcoICRI GAG'CTC
Cuts at: 854 854
Size: 3842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 490 616 1667 2099 2452 3114 102
Size: 388 126 1051 432 353 662 830
Fragments arranged by size:
1051 830 662 432 388 353 126
EcoNI CCTnn'n_nnAGG
Cuts at: 460 460
Size: 3842
EcoO109I rG'GnC_Cy
Cuts at: 465 518 519 591 663 734 815 981 465
Size: 53 1 72 72 71 81 166 3326
Fragments arranged by size:
3326 166 81 72 72 71 53 1
EcoRI G'AATT_C
Cuts at: 846 846
Size: 3842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3842
FspI TGC'GCA
Cuts at: 1623 1623
Size: 3842
HaeII r_GCGC'y
Cuts at: 795 1276 1524 2754 3525 795
Size: 481 248 1230 771 1112
Fragments arranged by size:
1230 1112 771 481 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 334 366 1684 1714 1716 1746 334
Size: 32 1318 30 2 30 2430
Fragments arranged by size:
2430 1318 32 30 30 2
HincII GTy'rAC
Cuts at: 881 881
Size: 3842
HindIII A'AGCT_T
Cuts at: 598 598
Size: 3842
KasI G'GCGC_C
Cuts at: 791 1272 1520 791
Size: 481 248 3113
Fragments arranged by size:
3113 481 248
KpnI G_GTAC'C
Cuts at: 862 862
Size: 3842
MluI A'CGCG_T
Cuts at: 3275 3275
Size: 3842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2601 2785 2601
Size: 184 3658
MscI TGG'CCA
Cuts at: 485 1603 485
Size: 1118 2724
MslI CAynn'nnrTG
Cuts at: 163 385 1831 1958 163
Size: 222 1446 127 2047
Fragments arranged by size:
2047 1446 222 127
MspA1I CmG'CkG
Cuts at: 423 953 1267 1627 2411 2656 423
Size: 530 314 360 784 245 1609
Fragments arranged by size:
1609 784 530 360 314 245
NaeI GCC'GGC
Cuts at: 618 709 2023 618
Size: 91 1314 2437
Fragments arranged by size:
2437 1314 91
NarI GG'CG_CC
Cuts at: 792 1273 1521 792
Size: 481 248 3113
Fragments arranged by size:
3113 481 248
NcoI C'CATG_G
Cuts at: 107 133 107
Size: 26 3816
NgoMIV G'CCGG_C
Cuts at: 616 707 2021 616
Size: 91 1314 2437
Fragments arranged by size:
2437 1314 91
NspI r_CATG'y
Cuts at: 148 895 1395 2998 148
Size: 747 500 1603 992
Fragments arranged by size:
1603 992 747 500
PasI CC'CwG_GG
Cuts at: 462 462
Size: 3842
PciI A'CATG_T
Cuts at: 2994 2994
Size: 3842
PmeI GTTT'AAAC
Cuts at: 841 841
Size: 3842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2288 2320 2288
Size: 32 3810
PspOMI G'GGCC_C
Cuts at: 518 662 734 815 518
Size: 144 72 81 3545
Fragments arranged by size:
3545 144 81 72
PspXI vC'TCGA_Gb
Cuts at: 742 742
Size: 3842
PstI C_TGCA'G
Cuts at: 889 1574 889
Size: 685 3157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3842
PvuII CAG'CTG
Cuts at: 423 1267 1627 423
Size: 844 360 2638
Fragments arranged by size:
2638 844 360
RsrII CG'GwC_CG
Cuts at: 2037 2037
Size: 3842
SacI G_AGCT'C
Cuts at: 856 856
Size: 3842
SalI G'TCGA_C
Cuts at: 879 879
Size: 3842
SapI GCTCTTCn'nnn_
Cuts at: 1865 2075 1865
Size: 210 3632
SbfI CC_TGCA'GG
Cuts at: 889 889
Size: 3842
ScaI AGT'ACT
Cuts at: 3359 3359
Size: 3842
SexAI A'CCwGG_T
Cuts at: 332 332
Size: 3842
SfcI C'TryA_G
Cuts at: 33 885 1570 2538 2729 33
Size: 852 685 968 191 1146
Fragments arranged by size:
1146 968 852 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 814 814
Size: 3842
SfoI GGC'GCC
Cuts at: 793 1274 1522 793
Size: 481 248 3113
Fragments arranged by size:
3113 481 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
SmaI CCC'GGG
Cuts at: 661 864 661
Size: 203 3639
SmlI C'TyrA_G
Cuts at: 742 997 1912 2626 2888 742
Size: 255 915 714 262 1696
Fragments arranged by size:
1696 915 714 262 255
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3842
SphI G_CATG'C
Cuts at: 895 1395 895
Size: 500 3342
StyI C'CwwG_G
Cuts at: 107 133 434 749 809 976 1330 107
Size: 26 301 315 60 167 354 2619
Fragments arranged by size:
2619 354 315 301 167 60 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1425 1558 2017 2226 1425
Size: 133 459 209 3041
Fragments arranged by size:
3041 459 209 133
TatI w'GTAC_w
Cuts at: 3357 3357
Size: 3842
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 131 163 3359 3391 131
Size: 32 3196 32 582
Fragments arranged by size:
3196 582 32 32
Tth111I GACn'n_nGTC
Cuts at: 1639 1639
Size: 3842
XbaI T'CTAG_A
Cuts at: 873 873
Size: 3842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1337 1337
Size: 3842
XhoI C'TCGA_G
Cuts at: 742 742
Size: 3842
XmaI C'CCGG_G
Cuts at: 659 862 659
Size: 203 3639
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PpuMI PshAI PsiI PsrI SacII
SanDI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI