Restriction map for
pF1KB9566
(Circular) (Six-base) MAPSORT of: pF1KB9566.seq Check: 2874 from: 1 to: 5528
pF1KB9566 5528 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 914 2184 2566 914
Size: 1270 382 3876
Fragments arranged by size:
3876 1270 382
Acc65I G'GTAC_C
Cuts at: 2544 2544
Size: 5528
AclI AA'CG_TT
Cuts at: 5368 5368
Size: 5528
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 461 689 2114 2456 3353 3785 4138 4800
Size: 24 228 1425 342 897 432 353 662
Cuts at: 4800 437
Size: 1165
Fragments arranged by size:
1425 1165 897 662 432 353 342 228
24
AflIII A'CryG_T
Cuts at: 501 525 543 552 561 570 2102 2145 2880
Size: 24 18 9 9 9 1532 43 735
Cuts at: 2880 4680 4961 501
Size: 1800 281 1068
Fragments arranged by size:
1800 1532 1068 735 281 43 24 18
9 9 9
AgeI A'CCGG_T
Cuts at: 2435 2435
Size: 5528
AhdI GACnn_n'nnGTC
Cuts at: 810 810
Size: 5528
AleI CACnn'nnGTG
Cuts at: 2007 2438 2007
Size: 431 5097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1991 2188 1991
Size: 197 5331
AlwNI CAG_nnn'CTG
Cuts at: 169 2833 4271 169
Size: 2664 1438 1426
Fragments arranged by size:
2664 1438 1426
ApaLI G'TGCA_C
Cuts at: 1132 4366 1132
Size: 3234 2294
ApoI r'AATT_y
Cuts at: 1672 2532 4881 5137 1672
Size: 860 2349 256 2063
Fragments arranged by size:
2349 2063 860 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5528
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5528
AvaI C'yCGr_G
Cuts at: 2548 2548
Size: 5528
BamHI G'GATC_C
Cuts at: 692 2553 692
Size: 1861 3667
BanI G'GyrC_C
Cuts at: 2544 2958 3206 3241 2544
Size: 414 248 35 4831
Fragments arranged by size:
4831 414 248 35
BanII G_rGCy'C
Cuts at: 1796 2542 3572 1796
Size: 746 1030 3752
Fragments arranged by size:
3752 1030 746
BbeI G_GCGC'C
Cuts at: 2962 3210 2962
Size: 248 5280
BbsI GAAGACnn'nnnn_
Cuts at: 1320 1930 2329 2472 1320
Size: 610 399 143 4376
Fragments arranged by size:
4376 610 399 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1441 1475 1441
Size: 34 5494
BciVI GTATCCnnnnn_n'
Cuts at: 1947 3426 4482 5528 1947
Size: 1479 1056 1046 1947
Fragments arranged by size:
1947 1479 1056 1046
BclI T'GATC_A
Cuts at: 192 942 963 3048 192
Size: 750 21 2085 2672
Fragments arranged by size:
2672 2085 750 21
BglII A'GATC_T
Cuts at: 3043 5080 3043
Size: 2037 3491
BlpI GC'TnA_GC
Cuts at: 2640 2640
Size: 5528
Bme1580I G_kGCm'C
Cuts at: 343 1136 1248 3153 3246 4370 343
Size: 793 112 1905 93 1124 1501
Fragments arranged by size:
1905 1501 1124 793 112 93
BmgBI CAC'GTC
Cuts at: 504 504
Size: 5528
BmrI ACTGGGnnnn_n'
Cuts at: 1829 2740 2821 3156 5300 1829
Size: 911 81 335 2144 2057
Fragments arranged by size:
2144 2057 911 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 162 3895 162
Size: 3733 1795
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 908 1552 908
Size: 644 4884
Bpu10I CC'TnA_GC
Cuts at: 389 389
Size: 5528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2025 2122 2704 3583 4297 4595 2025
Size: 97 582 879 714 298 2958
Fragments arranged by size:
2958 879 714 582 298 97
BsaI GGTCTCn'nnnn_
Cuts at: 5232 5232
Size: 5528
BsaAI yAC'GTr
Cuts at: 2148 2881 2148
Size: 733 4795
BsaBI GATnn'nnATC
Cuts at: 6 191 1364 3042 5079 6
Size: 185 1173 1678 2037 455
Fragments arranged by size:
2037 1678 1173 455 185
BsaHI Gr'CG_yC
Cuts at: 2959 3207 3909 5405 2959
Size: 248 702 1496 3082
Fragments arranged by size:
3082 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1226 2435 2717 3238 3916 4327 4474 4748 1226
Size: 1209 282 521 678 411 147 274 2006
Fragments arranged by size:
2006 1209 678 521 411 282 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1816 1846 1816
Size: 30 5498
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1111 1111
Size: 5528
BseYI C'CCAG_C
Cuts at: 2953 3711 4376 4784 2953
Size: 758 665 408 3697
Fragments arranged by size:
3697 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1987 1987
Size: 5528
BsiEI CG_ry'CG
Cuts at: 104 3070 3116 4346 104
Size: 2966 46 1230 1286
Fragments arranged by size:
2966 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1136 1796 2542 3320 3510 4370 1136
Size: 660 746 778 190 860 2294
Fragments arranged by size:
2294 860 778 746 660 190
BsmI GAATG_Cn'
Cuts at: 1579 1579
Size: 5528
Bsp1286I G_dGCh'C
Cuts at: 343 1136 1248 1796 2542 3153 3246 3320 3510
Size: 793 112 548 746 611 93 74 190
Cuts at: 3510 3572 4370 343
Size: 62 798 1501
Fragments arranged by size:
1501 798 793 746 611 548 190 112
93 74 62
BspHI T'CATG_A
Cuts at: 579 3960 579
Size: 3381 2147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2578 2792 3094 3475 2578
Size: 214 302 381 4631
Fragments arranged by size:
4631 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3820 3874 5358 5523 50
Size: 3770 54 1484 165 55
Fragments arranged by size:
3770 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 435 2016 3440 435
Size: 1581 1424 2523
Fragments arranged by size:
2523 1581 1424
BsrFI r'CCGG_y
Cuts at: 2435 3526 3707 2435
Size: 1091 181 4256
Fragments arranged by size:
4256 1091 181
BsrGI T'GTAC_A
Cuts at: 866 866
Size: 5528
BssSI C'ACGA_G
Cuts at: 1135 3799 4507 1135
Size: 2664 708 2156
Fragments arranged by size:
2664 2156 708
BstAPI GCAn_nnn'nTGC
Cuts at: 661 2493 2905 661
Size: 1832 412 3284
Fragments arranged by size:
3284 1832 412
BstBI TT'CG_AA
Cuts at: 2035 3889 2035
Size: 1854 3674
BstYI r'GATC_y
Cuts at: 1 692 2208 2253 2553 3020 3043 3378 3624
Size: 691 1516 45 300 467 23 335 246
Cuts at: 3624 3930 3942 4028 5080 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1516 1052 691 467 449 335 306 300
246 86 45 23 12
Bsu36I CC'TnA_GG
Cuts at: 851 2385 851
Size: 1534 3994
BtgI C'CryG_G
Cuts at: 107 800 107
Size: 693 4835
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1592 3478 3659 3822 1592
Size: 1886 181 163 3298
Fragments arranged by size:
3298 1886 181 163
BtsI GCAGTG_nn'
Cuts at: 1235 2771 1235
Size: 1536 3992
DraI TTT'AAA
Cuts at: 876 1046 2394 2527 876
Size: 170 1348 133 3877
Fragments arranged by size:
3877 1348 170 133
DraIII CAC_nnn'GTG
Cuts at: 2332 2332
Size: 5528
DrdI GACnn_nn'nnGTC
Cuts at: 3234 4578 3234
Size: 1344 4184
EaeI y'GGCC_r
Cuts at: 3113 3287 3678 3705 5050 3113
Size: 174 391 27 1345 3591
Fragments arranged by size:
3591 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3113 3113
Size: 5528
EarI CTCTTCn'nnn_
Cuts at: 1875 3551 3761 1875
Size: 1676 210 3642
Fragments arranged by size:
3642 1676 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3736 4478 4624 5338 3736
Size: 742 146 714 3926
Fragments arranged by size:
3926 742 714 146
EcoICRI GAG'CTC
Cuts at: 1794 2540 1794
Size: 746 4782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 461 689 908 1552 2114 2456 3353 3785
Size: 24 228 219 644 562 342 897 432
Cuts at: 3785 4138 4800 437
Size: 353 662 1165
Fragments arranged by size:
1165 897 662 644 562 432 353 342
228 219 24
EcoO109I rG'GnC_Cy
Cuts at: 2667 2667
Size: 5528
EcoRI G'AATT_C
Cuts at: 2532 2532
Size: 5528
EcoRV GAT'ATC
Cuts at: 348 348
Size: 5528
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 139 2475 3547 139
Size: 2336 1072 2120
Fragments arranged by size:
2336 2120 1072
FspI TGC'GCA
Cuts at: 3309 3309
Size: 5528
HaeII r_GCGC'y
Cuts at: 2962 3210 4440 5211 2962
Size: 248 1230 771 3279
Fragments arranged by size:
3279 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 649 681 796 828 1365 1397 2193 2225 3370
Size: 32 115 32 537 32 796 32 1145
Cuts at: 3370 3400 3402 3432 649
Size: 30 2 30 2745
Fragments arranged by size:
2745 1145 796 537 115 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2567 2567
Size: 5528
KasI G'GCGC_C
Cuts at: 2958 3206 2958
Size: 248 5280
KpnI G_GTAC'C
Cuts at: 2548 2548
Size: 5528
MluI A'CGCG_T
Cuts at: 4961 4961
Size: 5528
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 519 528 537 794 4287 4471 519
Size: 9 9 257 3493 184 1576
Fragments arranged by size:
3493 1576 257 184 9 9
MscI TGG'CCA
Cuts at: 3289 3289
Size: 5528
MslI CAynn'nnrTG
Cuts at: 185 256 584 862 1259 1754 1923 2007 2096
Size: 71 328 278 397 495 169 84 89
Cuts at: 2096 2265 2438 3517 3644 185
Size: 169 173 1079 127 2069
Fragments arranged by size:
2069 1079 495 397 328 278 173 169
169 127 89 84 71
MspA1I CmG'CkG
Cuts at: 1117 1478 1693 2639 2953 3313 4097 4342 1117
Size: 361 215 946 314 360 784 245 2303
Fragments arranged by size:
2303 946 784 361 360 314 245 215
NaeI GCC'GGC
Cuts at: 3709 3709
Size: 5528
NarI GG'CG_CC
Cuts at: 2959 3207 2959
Size: 248 5280
NcoI C'CATG_G
Cuts at: 107 800 107
Size: 693 4835
NdeI CA'TA_TG
Cuts at: 2318 2318
Size: 5528
NgoMIV G'CCGG_C
Cuts at: 3707 3707
Size: 5528
NspI r_CATG'y
Cuts at: 529 547 556 565 574 1891 1978 2106 2581
Size: 18 9 9 9 1317 87 128 475
Cuts at: 2581 3081 4684 529
Size: 500 1603 1373
Fragments arranged by size:
1603 1373 1317 500 475 128 87 18
9 9 9
PasI CC'CwG_GG
Cuts at: 989 989
Size: 5528
PciI A'CATG_T
Cuts at: 525 543 552 561 570 2102 4680 525
Size: 18 9 9 9 1532 2578 1373
Fragments arranged by size:
2578 1532 1373 18 9 9 9
PflMI CCAn_nnn'nTGG
Cuts at: 1722 1722
Size: 5528
PmeI GTTT'AAAC
Cuts at: 2527 2527
Size: 5528
PmlI CAC'GTG
Cuts at: 2148 2148
Size: 5528
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 3974
Size: 32 5496
PshAI GACnn'nnGTC
Cuts at: 1934 1934
Size: 5528
PstI C_TGCA'G
Cuts at: 1110 1866 2575 3260 1110
Size: 756 709 685 3378
Fragments arranged by size:
3378 756 709 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5528
PvuII CAG'CTG
Cuts at: 1693 2953 3313 1693
Size: 1260 360 3908
Fragments arranged by size:
3908 1260 360
RsrII CG'GwC_CG
Cuts at: 3723 3723
Size: 5528
SacI G_AGCT'C
Cuts at: 1796 2542 1796
Size: 746 4782
SalI G'TCGA_C
Cuts at: 2565 2565
Size: 5528
SapI GCTCTTCn'nnn_
Cuts at: 3551 3761 3551
Size: 210 5318
SbfI CC_TGCA'GG
Cuts at: 2575 2575
Size: 5528
ScaI AGT'ACT
Cuts at: 1846 2359 2446 5045 1846
Size: 513 87 2599 2329
Fragments arranged by size:
2599 2329 513 87
SfcI C'TryA_G
Cuts at: 33 911 1106 1862 2375 2571 3256 4224 4415
Size: 878 195 756 513 196 685 968 191
Cuts at: 4415 33
Size: 1146
Fragments arranged by size:
1146 968 878 756 685 513 196 195
191
SfoI GGC'GCC
Cuts at: 2960 3208 2960
Size: 248 5280
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5528
SgrAI Cr'CCGG_yG
Cuts at: 2435 2435
Size: 5528
SmaI CCC'GGG
Cuts at: 2550 2550
Size: 5528
SmlI C'TyrA_G
Cuts at: 2040 2137 2683 3598 4312 4574 2040
Size: 97 546 915 714 262 2994
Fragments arranged by size:
2994 915 714 546 262 97
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5528
SphI G_CATG'C
Cuts at: 2581 3081 2581
Size: 500 5028
StuI AGG'CCT
Cuts at: 2127 2127
Size: 5528
StyI C'CwwG_G
Cuts at: 107 752 800 2662 3016 107
Size: 645 48 1862 354 2619
Fragments arranged by size:
2619 1862 645 354 48
SwaI ATTT'AAAT
Cuts at: 1046 1046
Size: 5528
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3111 3244 3703 3912 3111
Size: 133 459 209 4727
Fragments arranged by size:
4727 459 209 133
TatI w'GTAC_w
Cuts at: 210 866 1480 1844 2357 2444 5043 210
Size: 656 614 364 513 87 2599 695
Fragments arranged by size:
2599 695 656 614 513 364 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2392 2424 5045 5077 2392
Size: 32 2621 32 2843
Fragments arranged by size:
2843 2621 32 32
Tth111I GACn'n_nGTC
Cuts at: 3325 3325
Size: 5528
XbaI T'CTAG_A
Cuts at: 2559 2559
Size: 5528
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 996 3023 996
Size: 2027 3501
XmaI C'CCGG_G
Cuts at: 2548 2548
Size: 5528
XmnI GAAnn'nnTTC
Cuts at: 332 1594 2458 2469 332
Size: 1262 864 11 3391
Fragments arranged by size:
3391 1262 864 11
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI BsiWI BsmBI BspEI
BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PfoI PpuMI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SspI XhoI ZraI