(Circular) (Six-base) MAPSORT of: pF1KB9566.seq Check: 2874 from: 1 to: 5528 pF1KB9566 5528 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AccI GT'mk_AC Cuts at: 914 2184 2566 914 Size: 1270 382 3876 Fragments arranged by size: 3876 1270 382 Acc65I G'GTAC_C Cuts at: 2544 2544 Size: 5528 AclI AA'CG_TT Cuts at: 5368 5368 Size: 5528 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 437 461 689 2114 2456 3353 3785 4138 4800 Size: 24 228 1425 342 897 432 353 662 Cuts at: 4800 437 Size: 1165 Fragments arranged by size: 1425 1165 897 662 432 353 342 228 24 AflIII A'CryG_T Cuts at: 501 525 543 552 561 570 2102 2145 2880 Size: 24 18 9 9 9 1532 43 735 Cuts at: 2880 4680 4961 501 Size: 1800 281 1068 Fragments arranged by size: 1800 1532 1068 735 281 43 24 18 9 9 9 AgeI A'CCGG_T Cuts at: 2435 2435 Size: 5528 AhdI GACnn_n'nnGTC Cuts at: 810 810 Size: 5528 AleI CACnn'nnGTG Cuts at: 2007 2438 2007 Size: 431 5097 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1991 2188 1991 Size: 197 5331 AlwNI CAG_nnn'CTG Cuts at: 169 2833 4271 169 Size: 2664 1438 1426 Fragments arranged by size: 2664 1438 1426 ApaLI G'TGCA_C Cuts at: 1132 4366 1132 Size: 3234 2294 ApoI r'AATT_y Cuts at: 1672 2532 4881 5137 1672 Size: 860 2349 256 2063 Fragments arranged by size: 2349 2063 860 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5528 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5528 AvaI C'yCGr_G Cuts at: 2548 2548 Size: 5528 BamHI G'GATC_C Cuts at: 692 2553 692 Size: 1861 3667 BanI G'GyrC_C Cuts at: 2544 2958 3206 3241 2544 Size: 414 248 35 4831 Fragments arranged by size: 4831 414 248 35 BanII G_rGCy'C Cuts at: 1796 2542 3572 1796 Size: 746 1030 3752 Fragments arranged by size: 3752 1030 746 BbeI G_GCGC'C Cuts at: 2962 3210 2962 Size: 248 5280 BbsI GAAGACnn'nnnn_ Cuts at: 1320 1930 2329 2472 1320 Size: 610 399 143 4376 Fragments arranged by size: 4376 610 399 143 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1441 1475 1441 Size: 34 5494 BciVI GTATCCnnnnn_n' Cuts at: 1947 3426 4482 5528 1947 Size: 1479 1056 1046 1947 Fragments arranged by size: 1947 1479 1056 1046 BclI T'GATC_A Cuts at: 192 942 963 3048 192 Size: 750 21 2085 2672 Fragments arranged by size: 2672 2085 750 21 BglII A'GATC_T Cuts at: 3043 5080 3043 Size: 2037 3491 BlpI GC'TnA_GC Cuts at: 2640 2640 Size: 5528 Bme1580I G_kGCm'C Cuts at: 343 1136 1248 3153 3246 4370 343 Size: 793 112 1905 93 1124 1501 Fragments arranged by size: 1905 1501 1124 793 112 93 BmgBI CAC'GTC Cuts at: 504 504 Size: 5528 BmrI ACTGGGnnnn_n' Cuts at: 1829 2740 2821 3156 5300 1829 Size: 911 81 335 2144 2057 Fragments arranged by size: 2144 2057 911 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 162 3895 162 Size: 3733 1795 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 908 1552 908 Size: 644 4884 Bpu10I CC'TnA_GC Cuts at: 389 389 Size: 5528 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2025 2122 2704 3583 4297 4595 2025 Size: 97 582 879 714 298 2958 Fragments arranged by size: 2958 879 714 582 298 97 BsaI GGTCTCn'nnnn_ Cuts at: 5232 5232 Size: 5528 BsaAI yAC'GTr Cuts at: 2148 2881 2148 Size: 733 4795 BsaBI GATnn'nnATC Cuts at: 6 191 1364 3042 5079 6 Size: 185 1173 1678 2037 455 Fragments arranged by size: 2037 1678 1173 455 185 BsaHI Gr'CG_yC Cuts at: 2959 3207 3909 5405 2959 Size: 248 702 1496 3082 Fragments arranged by size: 3082 1496 702 248 BsaWI w'CCGG_w Cuts at: 1226 2435 2717 3238 3916 4327 4474 4748 1226 Size: 1209 282 521 678 411 147 274 2006 Fragments arranged by size: 2006 1209 678 521 411 282 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1816 1846 1816 Size: 30 5498 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1111 1111 Size: 5528 BseYI C'CCAG_C Cuts at: 2953 3711 4376 4784 2953 Size: 758 665 408 3697 Fragments arranged by size: 3697 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1987 1987 Size: 5528 BsiEI CG_ry'CG Cuts at: 104 3070 3116 4346 104 Size: 2966 46 1230 1286 Fragments arranged by size: 2966 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 1136 1796 2542 3320 3510 4370 1136 Size: 660 746 778 190 860 2294 Fragments arranged by size: 2294 860 778 746 660 190 BsmI GAATG_Cn' Cuts at: 1579 1579 Size: 5528 Bsp1286I G_dGCh'C Cuts at: 343 1136 1248 1796 2542 3153 3246 3320 3510 Size: 793 112 548 746 611 93 74 190 Cuts at: 3510 3572 4370 343 Size: 62 798 1501 Fragments arranged by size: 1501 798 793 746 611 548 190 112 93 74 62 BspHI T'CATG_A Cuts at: 579 3960 579 Size: 3381 2147 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2578 2792 3094 3475 2578 Size: 214 302 381 4631 Fragments arranged by size: 4631 381 302 214 BsrBI CCG'CTC Cuts at: 50 3820 3874 5358 5523 50 Size: 3770 54 1484 165 55 Fragments arranged by size: 3770 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 435 2016 3440 435 Size: 1581 1424 2523 Fragments arranged by size: 2523 1581 1424 BsrFI r'CCGG_y Cuts at: 2435 3526 3707 2435 Size: 1091 181 4256 Fragments arranged by size: 4256 1091 181 BsrGI T'GTAC_A Cuts at: 866 866 Size: 5528 BssSI C'ACGA_G Cuts at: 1135 3799 4507 1135 Size: 2664 708 2156 Fragments arranged by size: 2664 2156 708 BstAPI GCAn_nnn'nTGC Cuts at: 661 2493 2905 661 Size: 1832 412 3284 Fragments arranged by size: 3284 1832 412 BstBI TT'CG_AA Cuts at: 2035 3889 2035 Size: 1854 3674 BstYI r'GATC_y Cuts at: 1 692 2208 2253 2553 3020 3043 3378 3624 Size: 691 1516 45 300 467 23 335 246 Cuts at: 3624 3930 3942 4028 5080 1 Size: 306 12 86 1052 449 Fragments arranged by size: 1516 1052 691 467 449 335 306 300 246 86 45 23 12 Bsu36I CC'TnA_GG Cuts at: 851 2385 851 Size: 1534 3994 BtgI C'CryG_G Cuts at: 107 800 107 Size: 693 4835 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1592 3478 3659 3822 1592 Size: 1886 181 163 3298 Fragments arranged by size: 3298 1886 181 163 BtsI GCAGTG_nn' Cuts at: 1235 2771 1235 Size: 1536 3992 DraI TTT'AAA Cuts at: 876 1046 2394 2527 876 Size: 170 1348 133 3877 Fragments arranged by size: 3877 1348 170 133 DraIII CAC_nnn'GTG Cuts at: 2332 2332 Size: 5528 DrdI GACnn_nn'nnGTC Cuts at: 3234 4578 3234 Size: 1344 4184 EaeI y'GGCC_r Cuts at: 3113 3287 3678 3705 5050 3113 Size: 174 391 27 1345 3591 Fragments arranged by size: 3591 1345 391 174 27 EagI C'GGCC_G Cuts at: 3113 3113 Size: 5528 EarI CTCTTCn'nnn_ Cuts at: 1875 3551 3761 1875 Size: 1676 210 3642 Fragments arranged by size: 3642 1676 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3736 4478 4624 5338 3736 Size: 742 146 714 3926 Fragments arranged by size: 3926 742 714 146 EcoICRI GAG'CTC Cuts at: 1794 2540 1794 Size: 746 4782 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 437 461 689 908 1552 2114 2456 3353 3785 Size: 24 228 219 644 562 342 897 432 Cuts at: 3785 4138 4800 437 Size: 353 662 1165 Fragments arranged by size: 1165 897 662 644 562 432 353 342 228 219 24 EcoO109I rG'GnC_Cy Cuts at: 2667 2667 Size: 5528 EcoRI G'AATT_C Cuts at: 2532 2532 Size: 5528 EcoRV GAT'ATC Cuts at: 348 348 Size: 5528 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 139 2475 3547 139 Size: 2336 1072 2120 Fragments arranged by size: 2336 2120 1072 FspI TGC'GCA Cuts at: 3309 3309 Size: 5528 HaeII r_GCGC'y Cuts at: 2962 3210 4440 5211 2962 Size: 248 1230 771 3279 Fragments arranged by size: 3279 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 649 681 796 828 1365 1397 2193 2225 3370 Size: 32 115 32 537 32 796 32 1145 Cuts at: 3370 3400 3402 3432 649 Size: 30 2 30 2745 Fragments arranged by size: 2745 1145 796 537 115 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2567 2567 Size: 5528 KasI G'GCGC_C Cuts at: 2958 3206 2958 Size: 248 5280 KpnI G_GTAC'C Cuts at: 2548 2548 Size: 5528 MluI A'CGCG_T Cuts at: 4961 4961 Size: 5528 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 519 528 537 794 4287 4471 519 Size: 9 9 257 3493 184 1576 Fragments arranged by size: 3493 1576 257 184 9 9 MscI TGG'CCA Cuts at: 3289 3289 Size: 5528 MslI CAynn'nnrTG Cuts at: 185 256 584 862 1259 1754 1923 2007 2096 Size: 71 328 278 397 495 169 84 89 Cuts at: 2096 2265 2438 3517 3644 185 Size: 169 173 1079 127 2069 Fragments arranged by size: 2069 1079 495 397 328 278 173 169 169 127 89 84 71 MspA1I CmG'CkG Cuts at: 1117 1478 1693 2639 2953 3313 4097 4342 1117 Size: 361 215 946 314 360 784 245 2303 Fragments arranged by size: 2303 946 784 361 360 314 245 215 NaeI GCC'GGC Cuts at: 3709 3709 Size: 5528 NarI GG'CG_CC Cuts at: 2959 3207 2959 Size: 248 5280 NcoI C'CATG_G Cuts at: 107 800 107 Size: 693 4835 NdeI CA'TA_TG Cuts at: 2318 2318 Size: 5528 NgoMIV G'CCGG_C Cuts at: 3707 3707 Size: 5528 NspI r_CATG'y Cuts at: 529 547 556 565 574 1891 1978 2106 2581 Size: 18 9 9 9 1317 87 128 475 Cuts at: 2581 3081 4684 529 Size: 500 1603 1373 Fragments arranged by size: 1603 1373 1317 500 475 128 87 18 9 9 9 PasI CC'CwG_GG Cuts at: 989 989 Size: 5528 PciI A'CATG_T Cuts at: 525 543 552 561 570 2102 4680 525 Size: 18 9 9 9 1532 2578 1373 Fragments arranged by size: 2578 1532 1373 18 9 9 9 PflMI CCAn_nnn'nTGG Cuts at: 1722 1722 Size: 5528 PmeI GTTT'AAAC Cuts at: 2527 2527 Size: 5528 PmlI CAC'GTG Cuts at: 2148 2148 Size: 5528 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 3974 Size: 32 5496 PshAI GACnn'nnGTC Cuts at: 1934 1934 Size: 5528 PstI C_TGCA'G Cuts at: 1110 1866 2575 3260 1110 Size: 756 709 685 3378 Fragments arranged by size: 3378 756 709 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 5528 PvuII CAG'CTG Cuts at: 1693 2953 3313 1693 Size: 1260 360 3908 Fragments arranged by size: 3908 1260 360 RsrII CG'GwC_CG Cuts at: 3723 3723 Size: 5528 SacI G_AGCT'C Cuts at: 1796 2542 1796 Size: 746 4782 SalI G'TCGA_C Cuts at: 2565 2565 Size: 5528 SapI GCTCTTCn'nnn_ Cuts at: 3551 3761 3551 Size: 210 5318 SbfI CC_TGCA'GG Cuts at: 2575 2575 Size: 5528 ScaI AGT'ACT Cuts at: 1846 2359 2446 5045 1846 Size: 513 87 2599 2329 Fragments arranged by size: 2599 2329 513 87 SfcI C'TryA_G Cuts at: 33 911 1106 1862 2375 2571 3256 4224 4415 Size: 878 195 756 513 196 685 968 191 Cuts at: 4415 33 Size: 1146 Fragments arranged by size: 1146 968 878 756 685 513 196 195 191 SfoI GGC'GCC Cuts at: 2960 3208 2960 Size: 248 5280 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5528 SgrAI Cr'CCGG_yG Cuts at: 2435 2435 Size: 5528 SmaI CCC'GGG Cuts at: 2550 2550 Size: 5528 SmlI C'TyrA_G Cuts at: 2040 2137 2683 3598 4312 4574 2040 Size: 97 546 915 714 262 2994 Fragments arranged by size: 2994 915 714 546 262 97 SpeI A'CTAG_T Cuts at: 67 67 Size: 5528 SphI G_CATG'C Cuts at: 2581 3081 2581 Size: 500 5028 StuI AGG'CCT Cuts at: 2127 2127 Size: 5528 StyI C'CwwG_G Cuts at: 107 752 800 2662 3016 107 Size: 645 48 1862 354 2619 Fragments arranged by size: 2619 1862 645 354 48 SwaI ATTT'AAAT Cuts at: 1046 1046 Size: 5528 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 3111 3244 3703 3912 3111 Size: 133 459 209 4727 Fragments arranged by size: 4727 459 209 133 TatI w'GTAC_w Cuts at: 210 866 1480 1844 2357 2444 5043 210 Size: 656 614 364 513 87 2599 695 Fragments arranged by size: 2599 695 656 614 513 364 87 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2392 2424 5045 5077 2392 Size: 32 2621 32 2843 Fragments arranged by size: 2843 2621 32 32 Tth111I GACn'n_nGTC Cuts at: 3325 3325 Size: 5528 XbaI T'CTAG_A Cuts at: 2559 2559 Size: 5528 XcmI CCAnnnn_n'nnnnTGG Cuts at: 996 3023 996 Size: 2027 3501 XmaI C'CCGG_G Cuts at: 2548 2548 Size: 5528 XmnI GAAnn'nnTTC Cuts at: 332 1594 2458 2469 332 Size: 1262 864 11 3391 Fragments arranged by size: 3391 1262 864 11 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AloI ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmtI BsiWI BsmBI BspEI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI NheI NotI NruI NsiI PacI PfoI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SnaBI SrfI SspI XhoI ZraI