Restriction map for
pF1KB9568
(Circular) (Six-base) MAPSORT of: pF1KB9568.seq Check: 5754 from: 1 to: 7448
pF1KB9568 7448 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1648 1648
Size: 7448
AatII G_ACGT'C
Cuts at: 875 2552 875
Size: 1677 5771
AccI GT'mk_AC
Cuts at: 4486 4486
Size: 7448
Acc65I G'GTAC_C
Cuts at: 3037 4464 3037
Size: 1427 6021
AclI AA'CG_TT
Cuts at: 758 1691 7288 758
Size: 933 5597 918
Fragments arranged by size:
5597 933 918
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 835 1537 3524 3743 5273 5705 6058 6720
Size: 105 702 1987 219 1530 432 353 662
Cuts at: 6720 730
Size: 1458
Fragments arranged by size:
1987 1530 1458 702 662 432 353 219
105
AfeI AGC'GCT
Cuts at: 2684 3024 2684
Size: 340 7108
AflIII A'CryG_T
Cuts at: 4800 6600 6881 4800
Size: 1800 281 5367
Fragments arranged by size:
5367 1800 281
AleI CACnn'nnGTG
Cuts at: 4150 4150
Size: 7448
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2359 2894 4349 2359
Size: 535 1455 5458
Fragments arranged by size:
5458 1455 535
AlwNI CAG_nnn'CTG
Cuts at: 785 813 1303 1803 2409 3286 4012 4753 6191
Size: 28 490 500 606 877 726 741 1438
Cuts at: 6191 785
Size: 2042
Fragments arranged by size:
2042 1438 877 741 726 606 500 490
28
ApaI G_GGCC'C
Cuts at: 1150 2365 1150
Size: 1215 6233
ApaLI G'TGCA_C
Cuts at: 4153 6286 4153
Size: 2133 5315
ApoI r'AATT_y
Cuts at: 2606 3595 4452 6801 7057 2606
Size: 989 857 2349 256 2997
Fragments arranged by size:
2997 2349 989 857 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7448
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7448
AvaI C'yCGr_G
Cuts at: 2009 3205 3385 4468 2009
Size: 1196 180 1083 4989
Fragments arranged by size:
4989 1196 1083 180
BamHI G'GATC_C
Cuts at: 1197 2235 4473 1197
Size: 1038 2238 4172
Fragments arranged by size:
4172 2238 1038
BanI G'GyrC_C
Cuts at: 667 1479 1711 3037 3141 3175 3327 4464 4878
Size: 812 232 1326 104 34 152 1137 414
Cuts at: 4878 5126 5161 667
Size: 248 35 2954
Fragments arranged by size:
2954 1326 1137 812 414 248 232 152
104 35 34
BanII G_rGCy'C
Cuts at: 335 605 1150 1787 2252 2327 2365 2717 2812
Size: 270 545 637 465 75 38 352 95
Cuts at: 2812 2932 3086 3445 3685 4462 5492 335
Size: 120 154 359 240 777 1030 2291
Fragments arranged by size:
2291 1030 777 637 545 465 359 352
270 240 154 120 95 75 38
BbeI G_GCGC'C
Cuts at: 3145 3331 4882 5130 3145
Size: 186 1551 248 5463
Fragments arranged by size:
5463 1551 248 186
BbsI GAAGACnn'nnnn_
Cuts at: 990 990
Size: 7448
BbvCI CC'TCA_GC
Cuts at: 3019 3019
Size: 7448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 777 811 2437 2471 3825 3859 777
Size: 34 1626 34 1354 34 4366
Fragments arranged by size:
4366 1626 1354 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3511 5346 6402 7448 3511
Size: 1835 1056 1046 3511
Fragments arranged by size:
3511 1835 1056 1046
BclI T'GATC_A
Cuts at: 4968 4968
Size: 7448
BfrBI ATG'CAT
Cuts at: 474 474
Size: 7448
BglI GCCn_nnn'nGGC
Cuts at: 505 3229 3365 505
Size: 2724 136 4588
Fragments arranged by size:
4588 2724 136
BglII A'GATC_T
Cuts at: 4963 7000 4963
Size: 2037 5411
BlpI GC'TnA_GC
Cuts at: 2152 2431 2722 4013 4560 2152
Size: 279 291 1291 547 5040
Fragments arranged by size:
5040 1291 547 291 279
Bme1580I G_kGCm'C
Cuts at: 1150 1365 1714 2365 4157 5073 5166 6290 1150
Size: 215 349 651 1792 916 93 1124 2308
Fragments arranged by size:
2308 1792 1124 916 651 349 215 93
BmgBI CAC'GTC
Cuts at: 2755 2755
Size: 7448
BmrI ACTGGGnnnn_n'
Cuts at: 908 3049 3178 4660 4741 5076 7220 908
Size: 2141 129 1482 81 335 2144 1136
Fragments arranged by size:
2144 2141 1482 1136 335 129 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2014 2341 2949 3699 5815 2014
Size: 327 608 750 2116 3647
Fragments arranged by size:
3647 2116 750 608 327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 2248 2288 2624 2642 3070 3444 3456 3806
Size: 1860 40 336 18 428 374 12 350
Cuts at: 3806 388
Size: 4030
Fragments arranged by size:
4030 1860 428 374 350 336 40 18
12
Bpu10I CC'TnA_GC
Cuts at: 2711 3019 4108 4376 2711
Size: 308 1089 268 5783
Fragments arranged by size:
5783 1089 308 268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 1414 4624 5503 6217 6515 214
Size: 1200 3210 879 714 298 1147
Fragments arranged by size:
3210 1200 1147 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2114 7152 2114
Size: 5038 2410
BsaAI yAC'GTr
Cuts at: 1476 1497 4801 1476
Size: 21 3304 4123
Fragments arranged by size:
4123 3304 21
BsaBI GATnn'nnATC
Cuts at: 6 787 4962 6999 6
Size: 781 4175 2037 455
Fragments arranged by size:
4175 2037 781 455
BsaHI Gr'CG_yC
Cuts at: 872 2549 2857 3142 3328 4879 5127 5829 7325
Size: 1677 308 285 186 1551 248 702 1496
Cuts at: 7325 872
Size: 995
Fragments arranged by size:
1677 1551 1496 995 702 308 285 248
186
BsaWI w'CCGG_w
Cuts at: 610 1722 3007 3034 3129 4214 4637 5158 5836
Size: 1112 1285 27 95 1085 423 521 678
Cuts at: 5836 6247 6394 6668 610
Size: 411 147 274 1390
Fragments arranged by size:
1390 1285 1112 1085 678 521 423 411
274 147 95 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2111 2141 2614 2644 4057 4087 2111
Size: 30 473 30 1413 30 5472
Fragments arranged by size:
5472 1413 473 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 202 1182 1590 2382 2922 2934 3223 3447
Size: 75 980 408 792 540 12 289 224
Cuts at: 3447 127
Size: 4128
Fragments arranged by size:
4128 980 792 540 408 289 224 75
12
BseYI C'CCAG_C
Cuts at: 775 806 1094 1796 1982 2089 2365 2458 2730
Size: 31 288 702 186 107 276 93 272
Cuts at: 2730 2988 3148 3259 4873 5631 6296 6704 775
Size: 258 160 111 1614 758 665 408 1519
Fragments arranged by size:
1614 1519 758 702 665 408 288 276
272 258 186 160 111 107 93 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 928 1139 1552 2874 184
Size: 744 211 413 1322 4758
Fragments arranged by size:
4758 1322 744 413 211
BsiEI CG_ry'CG
Cuts at: 104 3000 4990 5036 6266 104
Size: 2896 1990 46 1230 1286
Fragments arranged by size:
2896 1990 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 335 2158 2932 3086 3493 3862 4157 4462 5240
Size: 1823 774 154 407 369 295 305 778
Cuts at: 5240 5430 6290 335
Size: 190 860 1493
Fragments arranged by size:
1823 1493 860 778 774 407 369 305
295 190 154
BsiWI C'GTAC_G
Cuts at: 1103 1103
Size: 7448
BsmI GAATG_Cn'
Cuts at: 633 3906 633
Size: 3273 4175
BsmBI CGTCTCn'nnnn_
Cuts at: 492 1499 2551 2770 492
Size: 1007 1052 219 5170
Fragments arranged by size:
5170 1052 1007 219
Bsp1286I G_dGCh'C
Cuts at: 335 605 1150 1365 1714 1787 2158 2252 2327
Size: 270 545 215 349 73 371 94 75
Cuts at: 2327 2365 2717 2812 2932 3086 3445 3493 3685
Size: 38 352 95 120 154 359 48 192
Cuts at: 3685 3862 4157 4462 5073 5166 5240 5430 5492
Size: 177 295 305 611 93 74 190 62
Cuts at: 5492 6290 335
Size: 798 1493
Fragments arranged by size:
1493 798 611 545 371 359 352 349
305 295 270 215 192 190 177 154
120 95 94 93 75 74 73 62
48 38
BspEI T'CCGG_A
Cuts at: 610 1722 4214 610
Size: 1112 2492 3844
Fragments arranged by size:
3844 2492 1112
BspHI T'CATG_A
Cuts at: 4418 5880 4418
Size: 1462 5986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 206 1574 1648 2531 4498 4712 5014 5395 206
Size: 1368 74 883 1967 214 302 381 2259
Fragments arranged by size:
2259 1967 1368 883 381 302 214 74
BsrBI CCG'CTC
Cuts at: 50 857 1758 1764 2996 5740 5794 7278 7443
Size: 807 901 6 1232 2744 54 1484 165
Cuts at: 7443 50
Size: 55
Fragments arranged by size:
2744 1484 1232 901 807 165 55 54
6
BsrDI GCAATG_nn'
Cuts at: 530 4047 5360 530
Size: 3517 1313 2618
Fragments arranged by size:
3517 2618 1313
BsrFI r'CCGG_y
Cuts at: 175 698 801 2140 2999 4081 5446 5627 175
Size: 523 103 1339 859 1082 1365 181 1996
Fragments arranged by size:
1996 1365 1339 1082 859 523 181 103
BsrGI T'GTAC_A
Cuts at: 737 1040 737
Size: 303 7145
BssSI C'ACGA_G
Cuts at: 5719 6427 5719
Size: 708 6740
BstAPI GCAn_nnn'nTGC
Cuts at: 785 4825 785
Size: 4040 3408
BstBI TT'CG_AA
Cuts at: 5809 5809
Size: 7448
BstXI CCAn_nnnn'nTGG
Cuts at: 2290 2976 3741 2290
Size: 686 765 5997
Fragments arranged by size:
5997 765 686
BstYI r'GATC_y
Cuts at: 1 749 782 939 1197 1413 1701 2235 3957
Size: 748 33 157 258 216 288 534 1722
Cuts at: 3957 4473 4940 4963 5298 5544 5850 5862 5948
Size: 516 467 23 335 246 306 12 86
Cuts at: 5948 7000 1
Size: 1052 449
Fragments arranged by size:
1722 1052 748 534 516 467 449 335
306 288 258 246 216 157 86 33
23 12
BtgI C'CryG_G
Cuts at: 107 120 663 1050 2975 4337 107
Size: 13 543 387 1925 1362 3218
Fragments arranged by size:
3218 1925 1362 543 387 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 141 3639 5398 5579 5742 141
Size: 3498 1759 181 163 1847
Fragments arranged by size:
3498 1847 1759 181 163
BtsI GCAGTG_nn'
Cuts at: 423 1284 2058 4338 4691 423
Size: 861 774 2280 353 3180
Fragments arranged by size:
3180 2280 861 774 353
ClaI AT'CG_AT
Cuts at: 2597 2597
Size: 7448
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1443 1478 1443
Size: 35 7413
DraI TTT'AAA
Cuts at: 4447 4447
Size: 7448
DraIII CAC_nnn'GTG
Cuts at: 1056 1479 1575 2793 1056
Size: 423 96 1218 5711
Fragments arranged by size:
5711 1218 423 96
DrdI GACnn_nn'nnGTC
Cuts at: 5154 6498 5154
Size: 1344 6104
EaeI y'GGCC_r
Cuts at: 325 860 1232 1287 2997 3230 3731 3743 3794
Size: 535 372 55 1710 233 501 12 51
Cuts at: 3794 4083 5033 5207 5598 5625 6970 325
Size: 289 950 174 391 27 1345 803
Fragments arranged by size:
1710 1345 950 803 535 501 391 372
289 233 174 55 51 27 12
EagI C'GGCC_G
Cuts at: 2997 5033 2997
Size: 2036 5412
EarI CTCTTCn'nnn_
Cuts at: 1319 2940 4213 5471 5681 1319
Size: 1621 1273 1258 210 3086
Fragments arranged by size:
3086 1621 1273 1258 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 871 1815 3191 3646 5656 6398 6544 7258 871
Size: 944 1376 455 2010 742 146 714 1061
Fragments arranged by size:
2010 1376 1061 944 742 714 455 146
EcoICRI GAG'CTC
Cuts at: 333 2930 3084 4460 333
Size: 2597 154 1376 3321
Fragments arranged by size:
3321 2597 1376 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 730 835 1537 2248 2288 2624 2642 3070
Size: 342 105 702 711 40 336 18 428
Cuts at: 3070 3444 3456 3524 3743 3806 5273 5705 6058
Size: 374 12 68 219 63 1467 432 353
Cuts at: 6058 6720 388
Size: 662 1116
Fragments arranged by size:
1467 1116 711 702 662 432 428 374
353 342 336 219 105 68 63 40
18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 211 1305 1975 2351 4123 211
Size: 1094 670 376 1772 3536
Fragments arranged by size:
3536 1772 1094 670 376
EcoO109I rG'GnC_Cy
Cuts at: 507 1146 1147 1353 1466 1589 1609 1630 2161
Size: 639 1 206 113 123 20 21 531
Cuts at: 2161 2361 2362 2583 3213 3221 3719 4104 4587
Size: 200 1 221 630 8 498 385 483
Cuts at: 4587 507
Size: 3368
Fragments arranged by size:
3368 639 630 531 498 483 385 221
206 200 123 113 21 20 8 1
1
EcoRI G'AATT_C
Cuts at: 4452 4452
Size: 7448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4227 5467 4227
Size: 1240 6208
FseI GG_CCGG'CC
Cuts at: 3003 3003
Size: 7448
FspI TGC'GCA
Cuts at: 5229 5229
Size: 7448
HaeII r_GCGC'y
Cuts at: 500 1332 2686 3026 3145 3331 4882 5130 6360
Size: 832 1354 340 119 186 1551 248 1230
Cuts at: 6360 7131 500
Size: 771 817
Fragments arranged by size:
1551 1354 1230 832 817 771 340 248
186 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1431 1463 2769 2801 2836 2868 4057 4089 4179
Size: 32 1306 32 35 32 1189 32 90
Cuts at: 4179 4211 5290 5320 5322 5352 1431
Size: 32 1079 30 2 30 3527
Fragments arranged by size:
3527 1306 1189 1079 90 35 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4401 4487 4401
Size: 86 7362
HindIII A'AGCT_T
Cuts at: 295 295
Size: 7448
KasI G'GCGC_C
Cuts at: 3141 3327 4878 5126 3141
Size: 186 1551 248 5463
Fragments arranged by size:
5463 1551 248 186
KpnI G_GTAC'C
Cuts at: 3041 4468 3041
Size: 1427 6021
MluI A'CGCG_T
Cuts at: 6881 6881
Size: 7448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1361 6207 6391 1361
Size: 4846 184 2418
Fragments arranged by size:
4846 2418 184
MscI TGG'CCA
Cuts at: 327 3232 3733 3745 3796 5209 327
Size: 2905 501 12 51 1413 2566
Fragments arranged by size:
2905 2566 1413 501 51 12
MslI CAynn'nnrTG
Cuts at: 160 223 316 451 1499 3104 3658 3739 4150
Size: 63 93 135 1048 1605 554 81 411
Cuts at: 4150 5437 5564 160
Size: 1287 127 2044
Fragments arranged by size:
2044 1605 1287 1048 554 411 135 127
93 81 63
MspA1I CmG'CkG
Cuts at: 1389 1949 2469 2545 2721 3270 3485 3771 4012
Size: 560 520 76 176 549 215 286 241
Cuts at: 4012 4559 4873 5233 6017 6262 1389
Size: 547 314 360 784 245 2575
Fragments arranged by size:
2575 784 560 549 547 520 360 314
286 245 241 215 176 76
NaeI GCC'GGC
Cuts at: 177 700 803 2142 3001 5629 177
Size: 523 103 1339 859 2628 1996
Fragments arranged by size:
2628 1996 1339 859 523 103
NarI GG'CG_CC
Cuts at: 3142 3328 4879 5127 3142
Size: 186 1551 248 5463
Fragments arranged by size:
5463 1551 248 186
NcoI C'CATG_G
Cuts at: 107 120 2975 107
Size: 13 2855 4580
Fragments arranged by size:
4580 2855 13
NdeI CA'TA_TG
Cuts at: 367 367
Size: 7448
NgoMIV G'CCGG_C
Cuts at: 175 698 801 2140 2999 5627 175
Size: 523 103 1339 859 2628 1996
Fragments arranged by size:
2628 1996 1339 859 523 103
NsiI A_TGCA'T
Cuts at: 476 476
Size: 7448
NspI r_CATG'y
Cuts at: 228 412 474 4501 5001 6604 228
Size: 184 62 4027 500 1603 1072
Fragments arranged by size:
4027 1603 1072 500 184 62
PasI CC'CwG_GG
Cuts at: 2409 2911 4173 2409
Size: 502 1262 5684
Fragments arranged by size:
5684 1262 502
PciI A'CATG_T
Cuts at: 6600 6600
Size: 7448
PflMI CCAn_nnn'nTGG
Cuts at: 126 411 2955 3620 126
Size: 285 2544 665 3954
Fragments arranged by size:
3954 2544 665 285
PfoI T'CCnGG_A
Cuts at: 745 1416 2115 2225 4124 745
Size: 671 699 110 1899 4069
Fragments arranged by size:
4069 1899 699 671 110
PmeI GTTT'AAAC
Cuts at: 4447 4447
Size: 7448
PmlI CAC'GTG
Cuts at: 1476 1497 1476
Size: 21 7427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5894 5926 5894
Size: 32 7416
PpuMI rG'GwC_Cy
Cuts at: 1466 1589 1630 2583 3213 1466
Size: 123 41 953 630 5701
Fragments arranged by size:
5701 953 630 123 41
PspOMI G'GGCC_C
Cuts at: 1146 2361 1146
Size: 1215 6233
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 574 606 574
Size: 32 7416
PstI C_TGCA'G
Cuts at: 430 890 1959 2943 3365 3770 4495 5180 430
Size: 460 1069 984 422 405 725 685 2698
Fragments arranged by size:
2698 1069 984 725 685 460 422 405
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7448
PvuII CAG'CTG
Cuts at: 1389 2469 3771 4012 4873 5233 1389
Size: 1080 1302 241 861 360 3604
Fragments arranged by size:
3604 1302 1080 861 360 241
RsrII CG'GwC_CG
Cuts at: 5643 5643
Size: 7448
SacI G_AGCT'C
Cuts at: 335 2932 3086 4462 335
Size: 2597 154 1376 3321
Fragments arranged by size:
3321 2597 1376 154
SalI G'TCGA_C
Cuts at: 4485 4485
Size: 7448
SanDI GG'GwC_CC
Cuts at: 1466 1589 1630 1466
Size: 123 41 7284
Fragments arranged by size:
7284 123 41
SapI GCTCTTCn'nnn_
Cuts at: 1319 4213 5471 5681 1319
Size: 2894 1258 210 3086
Fragments arranged by size:
3086 2894 1258 210
SbfI CC_TGCA'GG
Cuts at: 3365 4495 3365
Size: 1130 6318
ScaI AGT'ACT
Cuts at: 6965 6965
Size: 7448
SexAI A'CCwGG_T
Cuts at: 254 2123 254
Size: 1869 5579
SfcI C'TryA_G
Cuts at: 33 208 426 886 1295 1955 2939 3361 3766
Size: 175 218 460 409 660 984 422 405
Cuts at: 3766 4491 5176 6144 6335 33
Size: 725 685 968 191 1146
Fragments arranged by size:
1146 984 968 725 685 660 460 422
409 405 218 191 175
SfiI GGCCn_nnn'nGGCC
Cuts at: 3229 3365 3229
Size: 136 7312
SfoI GGC'GCC
Cuts at: 3143 3329 4880 5128 3143
Size: 186 1551 248 5463
Fragments arranged by size:
5463 1551 248 186
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7448
SmaI CCC'GGG
Cuts at: 3387 4470 3387
Size: 1083 6365
SmlI C'TyrA_G
Cuts at: 229 1429 4603 5518 6232 6494 229
Size: 1200 3174 915 714 262 1183
Fragments arranged by size:
3174 1200 1183 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7448
SphI G_CATG'C
Cuts at: 228 4501 5001 228
Size: 4273 500 2675
Fragments arranged by size:
4273 2675 500
SrfI GCCC'GGGC
Cuts at: 3387 3387
Size: 7448
StuI AGG'CCT
Cuts at: 1840 3160 3359 1840
Size: 1320 199 5929
Fragments arranged by size:
5929 1320 199
StyI C'CwwG_G
Cuts at: 107 120 1584 1734 2975 3533 3893 3920 4253
Size: 13 1464 150 1241 558 360 27 333
Cuts at: 4253 4582 4936 107
Size: 329 354 2619
Fragments arranged by size:
2619 1464 1241 558 360 354 333 329
150 27 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1863 2943 5031 5164 5623 5832 1863
Size: 1080 2088 133 459 209 3479
Fragments arranged by size:
3479 2088 1080 459 209 133
TatI w'GTAC_w
Cuts at: 737 1040 1457 6963 737
Size: 303 417 5506 1222
Fragments arranged by size:
5506 1222 417 303
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 343 375 1704 1736 2614 2646 2997 3029 3746
Size: 32 1329 32 878 32 351 32 717
Cuts at: 3746 3778 4333 4365 6965 6997 343
Size: 32 555 32 2600 32 794
Fragments arranged by size:
2600 1329 878 794 717 555 351 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1593 5245 1593
Size: 3652 3796
XbaI T'CTAG_A
Cuts at: 4479 4479
Size: 7448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1091 1393 2025 2131 3918 4943 1091
Size: 302 632 106 1787 1025 3596
Fragments arranged by size:
3596 1787 1025 632 302 106
XmaI C'CCGG_G
Cuts at: 3385 4468 3385
Size: 1083 6365
XmnI GAAnn'nnTTC
Cuts at: 4207 4207
Size: 7448
ZraI GAC'GTC
Cuts at: 873 2550 873
Size: 1677 5771
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AloI AscI AvrII BaeI BmtI
BssHII BstEII BstZ17I Bsu36I EcoRV FspAI HpaI MfeI
NheI NotI NruI PacI PshAI PsiI PspXI SacII
SgrAI SnaBI SspI SwaI XhoI