Restriction map for
pF1KB9572
(Circular) (Six-base) MAPSORT of: pF1KB9572.seq Check: 4874 from: 1 to: 5477
pF1KB9572 5477 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 2515 2515
Size: 5477
Acc65I G'GTAC_C
Cuts at: 2493 2493
Size: 5477
AclI AA'CG_TT
Cuts at: 5317 5317
Size: 5477
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 320 443 888 932 2115 3302 3734 4481
Size: 21 123 445 44 1183 1187 432 747
Cuts at: 4481 4749 299
Size: 268 1027
Fragments arranged by size:
1187 1183 1027 747 445 432 268 123
44 21
AflIII A'CryG_T
Cuts at: 2829 3933 4910 2829
Size: 1104 977 3396
Fragments arranged by size:
3396 1104 977
AgeI A'CCGG_T
Cuts at: 3865 3865
Size: 5477
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 670 670
Size: 5477
AlwNI CAG_nnn'CTG
Cuts at: 132 367 739 2064 2088 2253 2782 4349 132
Size: 235 372 1325 24 165 529 1567 1260
Fragments arranged by size:
1567 1325 1260 529 372 235 165 24
ApaI G_GGCC'C
Cuts at: 212 2394 212
Size: 2182 3295
ApaLI G'TGCA_C
Cuts at: 4247 4247
Size: 5477
ApoI r'AATT_y
Cuts at: 507 542 1516 2481 4830 5086 507
Size: 35 974 965 2349 256 898
Fragments arranged by size:
2349 974 965 898 256 35
AseI AT'TA_AT
Cuts at: 20 20
Size: 5477
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5477
AvaI C'yCGr_G
Cuts at: 211 223 721 1592 2497 211
Size: 12 498 871 905 3191
Fragments arranged by size:
3191 905 871 498 12
BamHI G'GATC_C
Cuts at: 1386 2502 1386
Size: 1116 4361
BanI G'GyrC_C
Cuts at: 1702 2493 2907 3155 3190 1702
Size: 791 414 248 35 3989
Fragments arranged by size:
3989 791 414 248 35
BanII G_rGCy'C
Cuts at: 212 1054 1348 1831 2394 2491 3521 212
Size: 842 294 483 563 97 1030 2168
Fragments arranged by size:
2168 1030 842 563 483 294 97
BbeI G_GCGC'C
Cuts at: 2911 3159 2911
Size: 248 5229
BbsI GAAGACnn'nnnn_
Cuts at: 1757 1757
Size: 5477
BbvCI CC'TCA_GC
Cuts at: 1849 1849
Size: 5477
BciVI GTATCCnnnnn_n'
Cuts at: 3375 4136 5477 3375
Size: 761 1341 3375
Fragments arranged by size:
3375 1341 761
BclI T'GATC_A
Cuts at: 2997 2997
Size: 5477
BfrBI ATG'CAT
Cuts at: 1585 1585
Size: 5477
BglII A'GATC_T
Cuts at: 2992 5029 2992
Size: 2037 3440
BlpI GC'TnA_GC
Cuts at: 2589 2589
Size: 5477
Bme1580I G_kGCm'C
Cuts at: 212 779 877 2394 3102 3195 4251 212
Size: 567 98 1517 708 93 1056 1438
Fragments arranged by size:
1517 1438 1056 708 567 98 93
BmgBI CAC'GTC
Cuts at: 161 1679 1733 161
Size: 1518 54 3905
Fragments arranged by size:
3905 1518 54
BmrI ACTGGGnnnn_n'
Cuts at: 1150 1210 2689 2770 3105 5249 1150
Size: 60 1479 81 335 2144 1378
Fragments arranged by size:
2144 1479 1378 335 81 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1073 2354 3844 1073
Size: 1281 1490 2706
Fragments arranged by size:
2706 1490 1281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 1798 664
Size: 1134 4343
Bpu10I CC'TnA_GC
Cuts at: 1849 2204 2303 1849
Size: 355 99 5023
Fragments arranged by size:
5023 355 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 1045 1087 2653 3532 4024 4322 279
Size: 766 42 1566 879 492 298 1434
Fragments arranged by size:
1566 1434 879 766 492 298 42
BsaI GGTCTCn'nnnn_
Cuts at: 241 1939 5181 241
Size: 1698 3242 537
Fragments arranged by size:
3242 1698 537
BsaAI yAC'GTr
Cuts at: 2830 2830
Size: 5477
BsaBI GATnn'nnATC
Cuts at: 6 2991 5028 6
Size: 2985 2037 455
Fragments arranged by size:
2985 2037 455
BsaHI Gr'CG_yC
Cuts at: 674 2908 3156 3858 5354 674
Size: 2234 248 702 1496 797
Fragments arranged by size:
2234 1496 797 702 248
BsaWI w'CCGG_w
Cuts at: 2666 3187 3865 4139 4286 4697 2666
Size: 521 678 274 147 411 3446
Fragments arranged by size:
3446 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1140 1170 1140
Size: 30 5447
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 241 244 854 1054 1062 1675 2248 241
Size: 3 610 200 8 613 573 3470
Fragments arranged by size:
3470 613 610 573 200 8 3
BseYI C'CCAG_C
Cuts at: 778 1032 1282 2457 2902 3660 4237 4733 778
Size: 254 250 1175 445 758 577 496 1522
Fragments arranged by size:
1522 1175 758 577 496 445 254 250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 2354 163
Size: 2191 3286
BsiEI CG_ry'CG
Cuts at: 104 3019 3065 4273 104
Size: 2915 46 1208 1308
Fragments arranged by size:
2915 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1054 1877 2205 2491 3269 3459 4251 1054
Size: 823 328 286 778 190 792 2280
Fragments arranged by size:
2280 823 792 778 328 286 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1220 1723 1220
Size: 503 4974
Bsp1286I G_dGCh'C
Cuts at: 212 779 877 1054 1348 1831 1877 2205 2394
Size: 567 98 177 294 483 46 328 189
Cuts at: 2394 2491 3102 3195 3269 3459 3521 4251 212
Size: 97 611 93 74 190 62 730 1438
Fragments arranged by size:
1438 730 611 567 483 328 294 190
189 177 98 97 93 74 62 46
BspEI T'CCGG_A
Cuts at: 4697 4697
Size: 5477
BspHI T'CATG_A
Cuts at: 4653 4653
Size: 5477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1183 2527 2741 3043 3424 1183
Size: 1344 214 302 381 3236
Fragments arranged by size:
3236 1344 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3769 3823 5307 5472 50
Size: 3719 54 1484 165 55
Fragments arranged by size:
3719 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3389 3389
Size: 5477
BsrFI r'CCGG_y
Cuts at: 187 3475 3656 3865 187
Size: 3288 181 209 1799
Fragments arranged by size:
3288 1799 209 181
BssSI C'ACGA_G
Cuts at: 1223 3748 4106 1223
Size: 2525 358 2594
Fragments arranged by size:
2594 2525 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2854 2854
Size: 5477
BstBI TT'CG_AA
Cuts at: 2310 3838 2310
Size: 1528 3949
BstEII G'GTnAC_C
Cuts at: 714 714
Size: 5477
BstYI r'GATC_y
Cuts at: 1 128 1260 1386 1841 2502 2969 2992 3327
Size: 127 1132 126 455 661 467 23 335
Cuts at: 3327 3573 4585 4671 4683 5029 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1132 1012 661 467 455 449 346 335
246 127 126 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 820 820
Size: 5477
BtgI C'CryG_G
Cuts at: 107 121 584 1382 2069 107
Size: 14 463 798 687 3515
Fragments arranged by size:
3515 798 687 463 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 706 3427 3608 3771 706
Size: 2721 181 163 2412
Fragments arranged by size:
2721 2412 181 163
BtsI GCAGTG_nn'
Cuts at: 151 1425 1613 2720 151
Size: 1274 188 1107 2908
Fragments arranged by size:
2908 1274 1107 188
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1749 1784 1749
Size: 35 5442
DraI TTT'AAA
Cuts at: 1395 2476 1395
Size: 1081 4396
DrdI GACnn_nn'nnGTC
Cuts at: 3183 4041 3183
Size: 858 4619
EaeI y'GGCC_r
Cuts at: 1644 3062 3236 3627 3654 4999 1644
Size: 1418 174 391 27 1345 2122
Fragments arranged by size:
2122 1418 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3062 3062
Size: 5477
EarI CTCTTCn'nnn_
Cuts at: 908 1341 1886 1937 2044 2134 2264 2288 3500
Size: 433 545 51 107 90 130 24 1212
Cuts at: 3500 3710 908
Size: 210 2675
Fragments arranged by size:
2675 1212 545 433 210 130 107 90
51 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3685 3995 4141 5287 3685
Size: 310 146 1146 3875
Fragments arranged by size:
3875 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1052 2489 1052
Size: 1437 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 320 443 664 888 932 1798 2115 3302
Size: 21 123 221 224 44 866 317 1187
Cuts at: 3302 3734 4481 4749 299
Size: 432 747 268 1027
Fragments arranged by size:
1187 1027 866 747 432 317 268 224
221 123 44 21
EcoNI CCTnn'n_nnAGG
Cuts at: 941 941
Size: 5477
EcoO109I rG'GnC_Cy
Cuts at: 364 1797 2085 2114 2250 2390 2391 2616 364
Size: 1433 288 29 136 140 1 225 3225
Fragments arranged by size:
3225 1433 288 225 140 136 29 1
EcoRI G'AATT_C
Cuts at: 2481 2481
Size: 5477
EcoRV GAT'ATC
Cuts at: 591 591
Size: 5477
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3496 3496
Size: 5477
FspI TGC'GCA
Cuts at: 3258 3258
Size: 5477
HaeII r_GCGC'y
Cuts at: 2911 3159 4181 5160 2911
Size: 248 1022 979 3228
Fragments arranged by size:
3228 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 481 513 1989 2021 2086 2118 3319
Size: 32 211 32 1476 32 65 32 1201
Cuts at: 3319 3349 3351 3381 238
Size: 30 2 30 2334
Fragments arranged by size:
2334 1476 1201 211 65 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2516 2516
Size: 5477
HindIII A'AGCT_T
Cuts at: 1063 1126 1063
Size: 63 5414
KasI G'GCGC_C
Cuts at: 2907 3155 2907
Size: 248 5229
KpnI G_GTAC'C
Cuts at: 2497 2497
Size: 5477
MfeI C'AATT_G
Cuts at: 833 833
Size: 5477
MluI A'CGCG_T
Cuts at: 4910 4910
Size: 5477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 899 4148 4332 899
Size: 3249 184 2044
Fragments arranged by size:
3249 2044 184
MscI TGG'CCA
Cuts at: 1646 3238 1646
Size: 1592 3885
MslI CAynn'nnrTG
Cuts at: 626 1451 3466 3593 626
Size: 825 2015 127 2510
Fragments arranged by size:
2510 2015 825 127
MspA1I CmG'CkG
Cuts at: 1699 1870 2588 2902 3262 4275 4520 1699
Size: 171 718 314 360 1013 245 2656
Fragments arranged by size:
2656 1013 718 360 314 245 171
NaeI GCC'GGC
Cuts at: 189 3658 189
Size: 3469 2008
NarI GG'CG_CC
Cuts at: 2908 3156 2908
Size: 248 5229
NcoI C'CATG_G
Cuts at: 107 1382 2069 107
Size: 1275 687 3515
Fragments arranged by size:
3515 1275 687
NgoMIV G'CCGG_C
Cuts at: 187 3656 187
Size: 3469 2008
NsiI A_TGCA'T
Cuts at: 1587 1587
Size: 5477
NspI r_CATG'y
Cuts at: 1589 2530 3030 3937 1589
Size: 941 500 907 3129
Fragments arranged by size:
3129 941 907 500
PciI A'CATG_T
Cuts at: 3933 3933
Size: 5477
PflMI CCAn_nnn'nTGG
Cuts at: 1743 1743
Size: 5477
PfoI T'CCnGG_A
Cuts at: 680 680
Size: 5477
PmeI GTTT'AAAC
Cuts at: 2476 2476
Size: 5477
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4616 4648 4616
Size: 32 5445
PpuMI rG'GwC_Cy
Cuts at: 364 2114 364
Size: 1750 3727
PspOMI G'GGCC_C
Cuts at: 208 2390 208
Size: 2182 3295
PspXI vC'TCGA_Gb
Cuts at: 721 721
Size: 5477
PstI C_TGCA'G
Cuts at: 639 1319 1791 2399 2524 3209 639
Size: 680 472 608 125 685 2907
Fragments arranged by size:
2907 685 680 608 472 125
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5477
PvuII CAG'CTG
Cuts at: 1699 1870 2902 3262 1699
Size: 171 1032 360 3914
Fragments arranged by size:
3914 1032 360 171
RsrII CG'GwC_CG
Cuts at: 3672 3672
Size: 5477
SacI G_AGCT'C
Cuts at: 1054 2491 1054
Size: 1437 4040
SalI G'TCGA_C
Cuts at: 2514 2514
Size: 5477
SapI GCTCTTCn'nnn_
Cuts at: 2044 3500 3710 2044
Size: 1456 210 3811
Fragments arranged by size:
3811 1456 210
SbfI CC_TGCA'GG
Cuts at: 2524 2524
Size: 5477
ScaI AGT'ACT
Cuts at: 4994 4994
Size: 5477
SfcI C'TryA_G
Cuts at: 33 635 1208 1315 1787 2395 2520 3205 4198
Size: 602 573 107 472 608 125 685 993
Cuts at: 4198 4389 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 608 602 573 472 191
125 107
SfoI GGC'GCC
Cuts at: 2909 3157 2909
Size: 248 5229
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5477
SgrAI Cr'CCGG_yG
Cuts at: 187 187
Size: 5477
SmaI CCC'GGG
Cuts at: 2499 2499
Size: 5477
SmlI C'TyrA_G
Cuts at: 258 721 1060 1066 2632 3547 4039 4301 258
Size: 463 339 6 1566 915 492 262 1434
Fragments arranged by size:
1566 1434 915 492 463 339 262 6
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5477
SphI G_CATG'C
Cuts at: 2530 3030 2530
Size: 500 4977
StuI AGG'CCT
Cuts at: 727 727
Size: 5477
StyI C'CwwG_G
Cuts at: 107 887 1120 1382 2069 2611 2965 107
Size: 780 233 262 687 542 354 2619
Fragments arranged by size:
2619 780 687 542 354 262 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1485 3060 3193 3652 3861 1485
Size: 1575 133 459 209 3101
Fragments arranged by size:
3101 1575 459 209 133
TatI w'GTAC_w
Cuts at: 4992 4992
Size: 5477
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1140 1172 4994 5026 1140
Size: 32 3822 32 1591
Fragments arranged by size:
3822 1591 32 32
Tth111I GACn'n_nGTC
Cuts at: 162 3274 162
Size: 3112 2365
XbaI T'CTAG_A
Cuts at: 2508 2508
Size: 5477
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 864 2972 864
Size: 2108 3369
XhoI C'TCGA_G
Cuts at: 721 721
Size: 5477
XmaI C'CCGG_G
Cuts at: 2497 2497
Size: 5477
XmnI GAAnn'nnTTC
Cuts at: 2053 2053
Size: 5477
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BglI BmtI BsiWI BsmI BsrGI
BssHII BstXI BstZ17I ClaI DraIII FseI FspAI HpaI
NdeI NheI NotI NruI PacI PasI PmlI PshAI
PsiI PsrI SacII SanDI SexAI SfiI SnaBI SrfI
SspI SwaI ZraI