Restriction map for
pF1KB9574
(Circular) (Six-base) MAPSORT of: pF1KB9574.seq Check: 774 from: 1 to: 4253
pF1KB9574 4253 bp
With 182 enzymes: SgfI *
February 20, 2009 16:43 ..
AccI GT'mk_AC
Cuts at: 1291 1291
Size: 4253
Acc65I G'GTAC_C
Cuts at: 1269 1269
Size: 4253
AclI AA'CG_TT
Cuts at: 4093 4093
Size: 4253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 919 2078 2510 3257 3525 688
Size: 231 1159 432 747 268 1416
Fragments arranged by size:
1416 1159 747 432 268 231
AflIII A'CryG_T
Cuts at: 1118 1605 2709 3686 1118
Size: 487 1104 977 1685
Fragments arranged by size:
1685 1104 977 487
AgeI A'CCGG_T
Cuts at: 2641 2641
Size: 4253
AleI CACnn'nnGTG
Cuts at: 1084 1084
Size: 4253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 582 582
Size: 4253
AlwNI CAG_nnn'CTG
Cuts at: 333 1558 3125 333
Size: 1225 1567 1461
Fragments arranged by size:
1567 1461 1225
ApaI G_GGCC'C
Cuts at: 1052 1052
Size: 4253
ApaLI G'TGCA_C
Cuts at: 1077 3023 1077
Size: 1946 2307
ApoI r'AATT_y
Cuts at: 1257 3606 3862 1257
Size: 2349 256 1648
Fragments arranged by size:
2349 1648 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4253
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
AvaI C'yCGr_G
Cuts at: 117 1144 1273 117
Size: 1027 129 3097
Fragments arranged by size:
3097 1027 129
BamHI G'GATC_C
Cuts at: 1278 1278
Size: 4253
BanI G'GyrC_C
Cuts at: 1269 1683 1931 1966 1269
Size: 414 248 35 3556
Fragments arranged by size:
3556 414 248 35
BanII G_rGCy'C
Cuts at: 232 384 854 911 1052 1073 1267 2297 232
Size: 152 470 57 141 21 194 1030 2188
Fragments arranged by size:
2188 1030 470 194 152 141 57 21
BbeI G_GCGC'C
Cuts at: 1687 1935 1687
Size: 248 4005
BbsI GAAGACnn'nnnn_
Cuts at: 451 907 1115 1125 451
Size: 456 208 10 3579
Fragments arranged by size:
3579 456 208 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 698 732 698
Size: 34 4219
BciVI GTATCCnnnnn_n'
Cuts at: 2151 2912 4253 2151
Size: 761 1341 2151
Fragments arranged by size:
2151 1341 761
BclI T'GATC_A
Cuts at: 1773 1773
Size: 4253
BglII A'GATC_T
Cuts at: 1768 3805 1768
Size: 2037 2216
BlpI GC'TnA_GC
Cuts at: 1222 1365 1222
Size: 143 4110
Bme1580I G_kGCm'C
Cuts at: 1052 1081 1878 1971 3027 1052
Size: 29 797 93 1056 2278
Fragments arranged by size:
2278 1056 797 93 29
BmgBI CAC'GTC
Cuts at: 1121 1121
Size: 4253
BmrI ACTGGGnnnn_n'
Cuts at: 788 941 1023 1465 1546 1881 4025 788
Size: 153 82 442 81 335 2144 1016
Fragments arranged by size:
2144 1016 442 335 153 82 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1170 1227 2620 1170
Size: 57 1393 2803
Fragments arranged by size:
2803 1393 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 258 411 491 1087 204
Size: 54 153 80 596 3370
Fragments arranged by size:
3370 596 153 80 54
Bpu10I CC'TnA_GC
Cuts at: 848 848
Size: 4253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1429 2308 2800 3098 1429
Size: 879 492 298 2584
Fragments arranged by size:
2584 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3957 3957
Size: 4253
BsaAI yAC'GTr
Cuts at: 1606 1606
Size: 4253
BsaBI GATnn'nnATC
Cuts at: 6 1767 3804 6
Size: 1761 2037 455
Fragments arranged by size:
2037 1761 455
BsaHI Gr'CG_yC
Cuts at: 142 1684 1932 2634 4130 142
Size: 1542 248 702 1496 265
Fragments arranged by size:
1542 1496 702 265 248
BsaWI w'CCGG_w
Cuts at: 318 575 1083 1442 1963 2641 2915 3062 3473
Size: 257 508 359 521 678 274 147 411
Cuts at: 3473 318
Size: 1098
Fragments arranged by size:
1098 678 521 508 411 359 274 257
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 202 232 1018 1048 1060 1083 1090 1113 202
Size: 30 786 30 12 23 7 23 3342
Fragments arranged by size:
3342 786 30 30 23 23 12 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 345 594 826 829 345
Size: 249 232 3 3769
Fragments arranged by size:
3769 249 232 3
BseYI C'CCAG_C
Cuts at: 415 930 1102 1162 1678 2436 3013 3509 415
Size: 515 172 60 516 758 577 496 1159
Fragments arranged by size:
1159 758 577 516 515 496 172 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 358 1009 1092 1190 240
Size: 118 651 83 98 3303
Fragments arranged by size:
3303 651 118 98 83
BsiEI CG_ry'CG
Cuts at: 104 176 1795 1841 3049 104
Size: 72 1619 46 1208 1308
Fragments arranged by size:
1619 1308 1208 72 46
BsiHKAI G_wGCw'C
Cuts at: 1081 1267 2045 2235 3027 1081
Size: 186 778 190 792 2307
Fragments arranged by size:
2307 792 778 190 186
BsmBI CGTCTCn'nnnn_
Cuts at: 276 357 276
Size: 81 4172
Bsp1286I G_dGCh'C
Cuts at: 232 384 854 911 1052 1073 1081 1267 1878
Size: 152 470 57 141 21 8 186 611
Cuts at: 1878 1971 2045 2235 2297 3027 232
Size: 93 74 190 62 730 1458
Fragments arranged by size:
1458 730 611 470 190 186 152 141
93 74 62 57 21 8
BspEI T'CCGG_A
Cuts at: 318 3473 318
Size: 3155 1098
BspHI T'CATG_A
Cuts at: 3429 3429
Size: 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 332 671 720 1303 1517 1819 2200 332
Size: 339 49 583 214 302 381 2385
Fragments arranged by size:
2385 583 381 339 302 214 49
BsrBI CCG'CTC
Cuts at: 50 968 2545 2599 4083 4248 50
Size: 918 1577 54 1484 165 55
Fragments arranged by size:
1577 1484 918 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1206 2165 1206
Size: 959 3294
BsrFI r'CCGG_y
Cuts at: 2251 2432 2641 2251
Size: 181 209 3863
Fragments arranged by size:
3863 209 181
BssSI C'ACGA_G
Cuts at: 2524 2882 2524
Size: 358 3895
BstAPI GCAn_nnn'nTGC
Cuts at: 1630 1630
Size: 4253
BstBI TT'CG_AA
Cuts at: 2614 2614
Size: 4253
BstEII G'GTnAC_C
Cuts at: 1089 1089
Size: 4253
BstXI CCAn_nnnn'nTGG
Cuts at: 1065 1239 1065
Size: 174 4079
BstYI r'GATC_y
Cuts at: 1 722 1278 1745 1768 2103 2349 3361 3447
Size: 721 556 467 23 335 246 1012 86
Cuts at: 3447 3459 3805 1
Size: 12 346 449
Fragments arranged by size:
1012 721 556 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 293 1052 293
Size: 759 3494
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 353 2203 2384 2547 353
Size: 1850 181 163 2059
Fragments arranged by size:
2059 1850 181 163
BtsI GCAGTG_nn'
Cuts at: 299 334 1109 1496 299
Size: 35 775 387 3056
Fragments arranged by size:
3056 775 387 35
DraI TTT'AAA
Cuts at: 1252 1252
Size: 4253
DrdI GACnn_nn'nnGTC
Cuts at: 268 1959 2817 268
Size: 1691 858 1704
Fragments arranged by size:
1704 1691 858
EaeI y'GGCC_r
Cuts at: 173 266 1055 1838 2012 2403 2430 3775 173
Size: 93 789 783 174 391 27 1345 651
Fragments arranged by size:
1345 789 783 651 391 174 93 27
EagI C'GGCC_G
Cuts at: 173 1838 173
Size: 1665 2588
EarI CTCTTCn'nnn_
Cuts at: 512 743 2276 2486 512
Size: 231 1533 210 2279
Fragments arranged by size:
2279 1533 231 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2461 2771 2917 4063 2461
Size: 310 146 1146 2651
Fragments arranged by size:
2651 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1265 1265
Size: 4253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 258 411 491 688 919 1087 2078 2510
Size: 54 153 80 197 231 168 991 432
Cuts at: 2510 3257 3525 204
Size: 747 268 932
Fragments arranged by size:
991 932 747 432 268 231 197 168
153 80 54
EcoNI CCTnn'n_nnAGG
Cuts at: 667 790 667
Size: 123 4130
EcoO109I rG'GnC_Cy
Cuts at: 783 995 1048 1392 783
Size: 212 53 344 3644
Fragments arranged by size:
3644 344 212 53
EcoRI G'AATT_C
Cuts at: 1257 1257
Size: 4253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 4253
FspI TGC'GCA
Cuts at: 2034 2034
Size: 4253
HaeII r_GCGC'y
Cuts at: 1687 1935 2957 3936 1687
Size: 248 1022 979 2004
Fragments arranged by size:
2004 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 162 172 202 204 234 619 651 2095
Size: 32 10 30 2 30 385 32 1444
Cuts at: 2095 2125 2127 2157 130
Size: 30 2 30 2226
Fragments arranged by size:
2226 1444 385 32 32 30 30 30
30 10 2 2
HincII GTy'rAC
Cuts at: 240 1292 240
Size: 1052 3201
KasI G'GCGC_C
Cuts at: 1683 1931 1683
Size: 248 4005
KpnI G_GTAC'C
Cuts at: 1273 1273
Size: 4253
MluI A'CGCG_T
Cuts at: 3686 3686
Size: 4253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2924 3108 2924
Size: 184 4069
MscI TGG'CCA
Cuts at: 1057 2014 1057
Size: 957 3296
MslI CAynn'nnrTG
Cuts at: 1084 2242 2369 1084
Size: 1158 127 2968
Fragments arranged by size:
2968 1158 127
MspA1I CmG'CkG
Cuts at: 155 415 1106 1166 1174 1364 1678 2038 3051
Size: 260 691 60 8 190 314 360 1013
Cuts at: 3051 3296 155
Size: 245 1112
Fragments arranged by size:
1112 1013 691 360 314 260 245 190
60 8
NaeI GCC'GGC
Cuts at: 2434 2434
Size: 4253
NarI GG'CG_CC
Cuts at: 1684 1932 1684
Size: 248 4005
NcoI C'CATG_G
Cuts at: 1052 1052
Size: 4253
NgoMIV G'CCGG_C
Cuts at: 2432 2432
Size: 4253
NspI r_CATG'y
Cuts at: 1306 1806 2713 1306
Size: 500 907 2846
Fragments arranged by size:
2846 907 500
PasI CC'CwG_GG
Cuts at: 1038 1038
Size: 4253
PciI A'CATG_T
Cuts at: 2709 2709
Size: 4253
PmeI GTTT'AAAC
Cuts at: 1252 1252
Size: 4253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 4221
PpuMI rG'GwC_Cy
Cuts at: 995 995
Size: 4253
PspOMI G'GGCC_C
Cuts at: 1048 1048
Size: 4253
PstI C_TGCA'G
Cuts at: 160 329 561 1300 1985 160
Size: 169 232 739 685 2428
Fragments arranged by size:
2428 739 685 232 169
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4253
PvuII CAG'CTG
Cuts at: 1106 1166 1174 1678 2038 1106
Size: 60 8 504 360 3321
Fragments arranged by size:
3321 504 360 60 8
RsrII CG'GwC_CG
Cuts at: 2448 2448
Size: 4253
SacI G_AGCT'C
Cuts at: 1267 1267
Size: 4253
SalI G'TCGA_C
Cuts at: 1290 1290
Size: 4253
SapI GCTCTTCn'nnn_
Cuts at: 2276 2486 2276
Size: 210 4043
SbfI CC_TGCA'GG
Cuts at: 1300 1300
Size: 4253
ScaI AGT'ACT
Cuts at: 3770 3770
Size: 4253
SfcI C'TryA_G
Cuts at: 33 156 325 557 1296 1981 2974 3165 33
Size: 123 169 232 739 685 993 191 1121
Fragments arranged by size:
1121 993 739 685 232 191 169 123
SfoI GGC'GCC
Cuts at: 1685 1933 1685
Size: 248 4005
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
SmaI CCC'GGG
Cuts at: 1275 1275
Size: 4253
SmlI C'TyrA_G
Cuts at: 1408 2323 2815 3077 1408
Size: 915 492 262 2584
Fragments arranged by size:
2584 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4253
SphI G_CATG'C
Cuts at: 1306 1806 1306
Size: 500 3753
StuI AGG'CCT
Cuts at: 820 820
Size: 4253
StyI C'CwwG_G
Cuts at: 1052 1387 1741 1052
Size: 335 354 3564
Fragments arranged by size:
3564 354 335
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 664 919 1245 1836 1969 2428 2637 664
Size: 255 326 591 133 459 209 2280
Fragments arranged by size:
2280 591 459 326 255 209 133
TatI w'GTAC_w
Cuts at: 1096 3768 1096
Size: 2672 1581
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 313 345 1018 1050 1060 1083 1092 1115 3770
Size: 32 673 32 10 23 9 23 2655
Cuts at: 3770 3802 313
Size: 32 764
Fragments arranged by size:
2655 764 673 32 32 32 23 23
10 9
Tth111I GACn'n_nGTC
Cuts at: 2050 2050
Size: 4253
XbaI T'CTAG_A
Cuts at: 1284 1284
Size: 4253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 861 1748 861
Size: 887 3366
XmaI C'CCGG_G
Cuts at: 1273 1273
Size: 4253
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI BsiWI BsmI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI