Restriction map for
pF1KB9584
(Circular) (Six-base) MAPSORT of: pF1KB9584.seq Check: 3384 from: 1 to: 3923
pF1KB9584 3923 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 961 961
Size: 3923
Acc65I G'GTAC_C
Cuts at: 939 939
Size: 3923
AclI AA'CG_TT
Cuts at: 3763 3763
Size: 3923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1748 2180 2927 3195 1748
Size: 432 747 268 2476
Fragments arranged by size:
2476 747 432 268
AflIII A'CryG_T
Cuts at: 1275 2379 3356 1275
Size: 1104 977 1842
Fragments arranged by size:
1842 1104 977
AgeI A'CCGG_T
Cuts at: 2311 2311
Size: 3923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 469 545 469
Size: 76 3847
AlwNI CAG_nnn'CTG
Cuts at: 1228 2795 1228
Size: 1567 2356
ApaLI G'TGCA_C
Cuts at: 2693 2693
Size: 3923
ApoI r'AATT_y
Cuts at: 927 3276 3532 927
Size: 2349 256 1318
Fragments arranged by size:
2349 1318 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
AvaI C'yCGr_G
Cuts at: 207 357 615 654 943 207
Size: 150 258 39 289 3187
Fragments arranged by size:
3187 289 258 150 39
BamHI G'GATC_C
Cuts at: 948 948
Size: 3923
BanI G'GyrC_C
Cuts at: 154 350 939 1353 1601 1636 154
Size: 196 589 414 248 35 2441
Fragments arranged by size:
2441 589 414 248 196 35
BanII G_rGCy'C
Cuts at: 214 937 1967 214
Size: 723 1030 2170
Fragments arranged by size:
2170 1030 723
BbeI G_GCGC'C
Cuts at: 1357 1605 1357
Size: 248 3675
BciVI GTATCCnnnnn_n'
Cuts at: 1821 2582 3923 1821
Size: 761 1341 1821
Fragments arranged by size:
1821 1341 761
BclI T'GATC_A
Cuts at: 1443 1443
Size: 3923
BglI GCCn_nnn'nGGC
Cuts at: 376 428 721 376
Size: 52 293 3578
Fragments arranged by size:
3578 293 52
BglII A'GATC_T
Cuts at: 1438 3475 1438
Size: 2037 1886
BlpI GC'TnA_GC
Cuts at: 1035 1035
Size: 3923
Bme1580I G_kGCm'C
Cuts at: 353 1548 1641 2697 353
Size: 1195 93 1056 1579
Fragments arranged by size:
1579 1195 1056 93
BmgBI CAC'GTC
Cuts at: 729 729
Size: 3923
BmrI ACTGGGnnnn_n'
Cuts at: 390 1135 1216 1551 3695 390
Size: 745 81 335 2144 618
Fragments arranged by size:
2144 745 618 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 3923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1099 1978 2470 2768 1099
Size: 879 492 298 2254
Fragments arranged by size:
2254 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3627 3627
Size: 3923
BsaAI yAC'GTr
Cuts at: 1276 1276
Size: 3923
BsaBI GATnn'nnATC
Cuts at: 6 1437 3474 6
Size: 1431 2037 455
Fragments arranged by size:
2037 1431 455
BsaHI Gr'CG_yC
Cuts at: 1354 1602 2304 3800 1354
Size: 248 702 1496 1477
Fragments arranged by size:
1496 1477 702 248
BsaWI w'CCGG_w
Cuts at: 1112 1633 2311 2585 2732 3143 1112
Size: 521 678 274 147 411 1892
Fragments arranged by size:
1892 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 239 849 901 1348 2106 2683 3179 239
Size: 610 52 447 758 577 496 983
Fragments arranged by size:
983 758 610 577 496 447 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 151
Size: 3923
BsiEI CG_ry'CG
Cuts at: 104 1465 1511 2719 104
Size: 1361 46 1208 1308
Fragments arranged by size:
1361 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 272 937 1715 1905 2697 272
Size: 665 778 190 792 1498
Fragments arranged by size:
1498 792 778 665 190
BsmI GAATG_Cn'
Cuts at: 303 303
Size: 3923
Bsp1286I G_dGCh'C
Cuts at: 214 272 353 937 1548 1641 1715 1905 1967
Size: 58 81 584 611 93 74 190 62
Cuts at: 1967 2697 214
Size: 730 1440
Fragments arranged by size:
1440 730 611 584 190 93 81 74
62 58
BspEI T'CCGG_A
Cuts at: 3143 3143
Size: 3923
BspHI T'CATG_A
Cuts at: 3099 3099
Size: 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 973 1187 1489 1870 973
Size: 214 302 381 3026
Fragments arranged by size:
3026 381 302 214
BsrBI CCG'CTC
Cuts at: 50 308 2215 2269 3753 3918 50
Size: 258 1907 54 1484 165 55
Fragments arranged by size:
1907 1484 258 165 55 54
BsrDI GCAATG_nn'
Cuts at: 746 1835 746
Size: 1089 2834
BsrFI r'CCGG_y
Cuts at: 438 1921 2102 2311 438
Size: 1483 181 209 2050
Fragments arranged by size:
2050 1483 209 181
BssHII G'CGCG_C
Cuts at: 362 362
Size: 3923
BssSI C'ACGA_G
Cuts at: 303 2194 2552 303
Size: 1891 358 1674
Fragments arranged by size:
1891 1674 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1300 1300
Size: 3923
BstBI TT'CG_AA
Cuts at: 2284 2284
Size: 3923
BstYI r'GATC_y
Cuts at: 1 948 1415 1438 1773 2019 3031 3117 3129
Size: 947 467 23 335 246 1012 86 12
Cuts at: 3129 3475 1
Size: 346 449
Fragments arranged by size:
1012 947 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 135 374 719 107
Size: 28 239 345 3311
Fragments arranged by size:
3311 345 239 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 337 1873 2054 2217 337
Size: 1536 181 163 2043
Fragments arranged by size:
2043 1536 181 163
BtsI GCAGTG_nn'
Cuts at: 1166 1166
Size: 3923
DraI TTT'AAA
Cuts at: 922 922
Size: 3923
DrdI GACnn_nn'nnGTC
Cuts at: 1629 2487 1629
Size: 858 3065
EaeI y'GGCC_r
Cuts at: 125 377 416 440 1508 1682 2073 2100 3445
Size: 252 39 24 1068 174 391 27 1345
Cuts at: 3445 125
Size: 603
Fragments arranged by size:
1345 1068 603 391 252 174 39 27
24
EagI C'GGCC_G
Cuts at: 1508 1508
Size: 3923
EarI CTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2131 2441 2587 3733 2131
Size: 310 146 1146 2321
Fragments arranged by size:
2321 1146 310 146
EcoICRI GAG'CTC
Cuts at: 935 935
Size: 3923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1748 2180 2927 3195 1748
Size: 432 747 268 2476
Fragments arranged by size:
2476 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 530 530
Size: 3923
EcoO109I rG'GnC_Cy
Cuts at: 406 1062 406
Size: 656 3267
EcoRI G'AATT_C
Cuts at: 927 927
Size: 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3923
FspI TGC'GCA
Cuts at: 1704 1704
Size: 3923
HaeII r_GCGC'y
Cuts at: 144 1357 1605 2627 3606 144
Size: 1213 248 1022 979 461
Fragments arranged by size:
1213 1022 979 461 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 201 233 1765 1795 1797 1827 201
Size: 32 1532 30 2 30 2297
Fragments arranged by size:
2297 1532 32 30 30 2
HincII GTy'rAC
Cuts at: 962 962
Size: 3923
KasI G'GCGC_C
Cuts at: 1353 1601 1353
Size: 248 3675
KpnI G_GTAC'C
Cuts at: 943 943
Size: 3923
MluI A'CGCG_T
Cuts at: 3356 3356
Size: 3923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2594 2778 2594
Size: 184 3739
MscI TGG'CCA
Cuts at: 127 418 1684 127
Size: 291 1266 2366
Fragments arranged by size:
2366 1266 291
MslI CAynn'nnrTG
Cuts at: 193 1912 2039 193
Size: 1719 127 2077
Fragments arranged by size:
2077 1719 127
MspA1I CmG'CkG
Cuts at: 376 453 590 683 695 701 736 1034 1348
Size: 77 137 93 12 6 35 298 314
Cuts at: 1348 1708 2721 2966 376
Size: 360 1013 245 1333
Fragments arranged by size:
1333 1013 360 314 298 245 137 93
77 35 12 6
NaeI GCC'GGC
Cuts at: 440 2104 440
Size: 1664 2259
NarI GG'CG_CC
Cuts at: 1354 1602 1354
Size: 248 3675
NcoI C'CATG_G
Cuts at: 107 107
Size: 3923
NdeI CA'TA_TG
Cuts at: 119 119
Size: 3923
NgoMIV G'CCGG_C
Cuts at: 438 2102 438
Size: 1664 2259
NspI r_CATG'y
Cuts at: 322 450 513 711 976 1476 2383 322
Size: 128 63 198 265 500 907 1862
Fragments arranged by size:
1862 907 500 265 198 128 63
PciI A'CATG_T
Cuts at: 2379 2379
Size: 3923
PflMI CCAn_nnn'nTGG
Cuts at: 123 189 402 123
Size: 66 213 3644
Fragments arranged by size:
3644 213 66
PmeI GTTT'AAAC
Cuts at: 922 922
Size: 3923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 3891
PpuMI rG'GwC_Cy
Cuts at: 406 406
Size: 3923
PstI C_TGCA'G
Cuts at: 458 534 970 1655 458
Size: 76 436 685 2726
Fragments arranged by size:
2726 685 436 76
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3923
PvuII CAG'CTG
Cuts at: 453 1348 1708 453
Size: 895 360 2668
Fragments arranged by size:
2668 895 360
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 3923
SacI G_AGCT'C
Cuts at: 937 937
Size: 3923
SacII CC_GC'GG
Cuts at: 377 377
Size: 3923
SalI G'TCGA_C
Cuts at: 960 960
Size: 3923
SanDI GG'GwC_CC
Cuts at: 406 406
Size: 3923
SapI GCTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
SbfI CC_TGCA'GG
Cuts at: 970 970
Size: 3923
ScaI AGT'ACT
Cuts at: 3440 3440
Size: 3923
SfcI C'TryA_G
Cuts at: 33 454 530 966 1651 2644 2835 33
Size: 421 76 436 685 993 191 1121
Fragments arranged by size:
1121 993 685 436 421 191 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 376 376
Size: 3923
SfoI GGC'GCC
Cuts at: 1355 1603 1355
Size: 248 3675
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
SmaI CCC'GGG
Cuts at: 945 945
Size: 3923
SmlI C'TyrA_G
Cuts at: 1078 1993 2485 2747 1078
Size: 915 492 262 2254
Fragments arranged by size:
2254 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3923
SphI G_CATG'C
Cuts at: 322 450 976 1476 322
Size: 128 526 500 2769
Fragments arranged by size:
2769 526 500 128
StyI C'CwwG_G
Cuts at: 107 797 1057 1411 107
Size: 690 260 354 2619
Fragments arranged by size:
2619 690 354 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1506 1639 2098 2307 1506
Size: 133 459 209 3122
Fragments arranged by size:
3122 459 209 133
TatI w'GTAC_w
Cuts at: 3438 3438
Size: 3923
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 871 903 3440 3472 871
Size: 32 2537 32 1322
Fragments arranged by size:
2537 1322 32 32
Tth111I GACn'n_nGTC
Cuts at: 1720 1720
Size: 3923
XbaI T'CTAG_A
Cuts at: 954 954
Size: 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1418 1418
Size: 3923
XmaI C'CCGG_G
Cuts at: 943 943
Size: 3923
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SexAI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI