Restriction map for
pF1KB9585
(Circular) (Six-base) MAPSORT of: pF1KB9585.seq Check: 420 from: 1 to: 4148
pF1KB9585 4148 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1186 1186
Size: 4148
Acc65I G'GTAC_C
Cuts at: 1164 1164
Size: 4148
AclI AA'CG_TT
Cuts at: 3988 3988
Size: 4148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 1973 2405 3152 3420 315
Size: 1658 432 747 268 1043
Fragments arranged by size:
1658 1043 747 432 268
AflIII A'CryG_T
Cuts at: 1500 2604 3581 1500
Size: 1104 977 2067
Fragments arranged by size:
2067 1104 977
AgeI A'CCGG_T
Cuts at: 2536 2536
Size: 4148
AlwNI CAG_nnn'CTG
Cuts at: 1453 3020 1453
Size: 1567 2581
ApaLI G'TGCA_C
Cuts at: 2918 2918
Size: 4148
ApoI r'AATT_y
Cuts at: 1152 3501 3757 1152
Size: 2349 256 1543
Fragments arranged by size:
2349 1543 256
AscI GG'CGCG_CC
Cuts at: 356 356
Size: 4148
AseI AT'TA_AT
Cuts at: 20 20
Size: 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
AvaI C'yCGr_G
Cuts at: 268 1111 1168 268
Size: 843 57 3248
Fragments arranged by size:
3248 843 57
BamHI G'GATC_C
Cuts at: 1173 1173
Size: 4148
BanI G'GyrC_C
Cuts at: 249 372 536 681 753 834 870 1164 1578
Size: 123 164 145 72 81 36 294 414
Cuts at: 1578 1826 1861 249
Size: 248 35 2536
Fragments arranged by size:
2536 414 294 248 164 145 123 81
72 36 35
BanII G_rGCy'C
Cuts at: 626 650 820 1025 1079 1118 1162 2192 626
Size: 24 170 205 54 39 44 1030 2582
Fragments arranged by size:
2582 1030 205 170 54 44 39 24
BbeI G_GCGC'C
Cuts at: 253 376 540 685 838 874 1582 1830 253
Size: 123 164 145 153 36 708 248 2571
Fragments arranged by size:
2571 708 248 164 153 145 123 36
BbsI GAAGACnn'nnnn_
Cuts at: 907 907
Size: 4148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 936 970 90
Size: 34 812 34 3268
Fragments arranged by size:
3268 812 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2046 2807 4148 2046
Size: 761 1341 2046
Fragments arranged by size:
2046 1341 761
BclI T'GATC_A
Cuts at: 1668 1668
Size: 4148
BglI GCCn_nnn'nGGC
Cuts at: 247 806 868 247
Size: 559 62 3527
Fragments arranged by size:
3527 559 62
BglII A'GATC_T
Cuts at: 1663 3700 1663
Size: 2037 2111
BlpI GC'TnA_GC
Cuts at: 1078 1260 1078
Size: 182 3966
Bme1580I G_kGCm'C
Cuts at: 1773 1866 2922 1773
Size: 93 1056 2999
Fragments arranged by size:
2999 1056 93
BmgBI CAC'GTC
Cuts at: 785 785
Size: 4148
BmrI ACTGGGnnnn_n'
Cuts at: 1360 1441 1776 3920 1360
Size: 81 335 2144 1588
Fragments arranged by size:
2144 1588 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1018 1039 364
Size: 654 21 3473
Fragments arranged by size:
3473 654 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 1324 2203 2695 2993 610
Size: 714 879 492 298 1765
Fragments arranged by size:
1765 879 714 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3852 3852
Size: 4148
BsaAI yAC'GTr
Cuts at: 525 1501 525
Size: 976 3172
BsaBI GATnn'nnATC
Cuts at: 6 1662 3699 6
Size: 1656 2037 455
Fragments arranged by size:
2037 1656 455
BsaHI Gr'CG_yC
Cuts at: 250 373 537 682 827 835 871 1579 1827
Size: 123 164 145 145 8 36 708 248
Cuts at: 1827 2529 4025 250
Size: 702 1496 373
Fragments arranged by size:
1496 708 702 373 248 164 145 145
123 36 8
BsaWI w'CCGG_w
Cuts at: 1337 1858 2536 2810 2957 3368 1337
Size: 521 678 274 147 411 2117
Fragments arranged by size:
2117 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 445 457 475 487 1051 1081 445
Size: 12 18 12 564 30 3512
Fragments arranged by size:
3512 564 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 426 685 780 1119 426
Size: 259 95 339 3455
Fragments arranged by size:
3455 339 259 95
BseYI C'CCAG_C
Cuts at: 132 294 351 1573 2331 2908 3404 132
Size: 162 57 1222 758 577 496 876
Fragments arranged by size:
1222 876 758 577 496 162 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 571 547
Size: 24 4124
BsiEI CG_ry'CG
Cuts at: 104 512 845 1031 1690 1736 2944 104
Size: 408 333 186 659 46 1208 1308
Fragments arranged by size:
1308 1208 659 408 333 186 46
BsiHKAI G_wGCw'C
Cuts at: 626 1025 1079 1162 1940 2130 2922 626
Size: 399 54 83 778 190 792 1852
Fragments arranged by size:
1852 792 778 399 190 83 54
BsiWI C'GTAC_G
Cuts at: 224 719 224
Size: 495 3653
BsmBI CGTCTCn'nnnn_
Cuts at: 994 994
Size: 4148
Bsp1286I G_dGCh'C
Cuts at: 626 650 820 1025 1079 1118 1162 1773 1866
Size: 24 170 205 54 39 44 611 93
Cuts at: 1866 1940 2130 2192 2922 626
Size: 74 190 62 730 1852
Fragments arranged by size:
1852 730 611 205 190 170 93 74
62 54 44 39 24
BspEI T'CCGG_A
Cuts at: 3368 3368
Size: 4148
BspHI T'CATG_A
Cuts at: 3324 3324
Size: 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 870 1198 1412 1714 2095 870
Size: 328 214 302 381 2923
Fragments arranged by size:
2923 381 328 302 214
BsrBI CCG'CTC
Cuts at: 50 761 1041 2440 2494 3978 4143 50
Size: 711 280 1399 54 1484 165 55
Fragments arranged by size:
1484 1399 711 280 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2060 2060
Size: 4148
BsrFI r'CCGG_y
Cuts at: 286 507 867 2146 2327 2536 286
Size: 221 360 1279 181 209 1898
Fragments arranged by size:
1898 1279 360 221 209 181
BssHII G'CGCG_C
Cuts at: 318 356 557 729 318
Size: 38 201 172 3737
Fragments arranged by size:
3737 201 172 38
BssSI C'ACGA_G
Cuts at: 313 2419 2777 313
Size: 2106 358 1684
Fragments arranged by size:
2106 1684 358
BstAPI GCAn_nnn'nTGC
Cuts at: 497 1525 497
Size: 1028 3120
BstBI TT'CG_AA
Cuts at: 2509 2509
Size: 4148
BstXI CCAn_nnnn'nTGG
Cuts at: 183 183
Size: 4148
BstYI r'GATC_y
Cuts at: 1 932 1173 1640 1663 1998 2244 3256 3342
Size: 931 241 467 23 335 246 1012 86
Cuts at: 3342 3354 3700 1
Size: 12 346 449
Fragments arranged by size:
1012 931 467 449 346 335 246 241
86 23 12
BtgI C'CryG_G
Cuts at: 176 245 257 380 386 650 905 176
Size: 69 12 123 6 264 255 3419
Fragments arranged by size:
3419 264 255 123 69 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 939 2098 2279 2442 939
Size: 1159 181 163 2645
Fragments arranged by size:
2645 1159 181 163
BtsI GCAGTG_nn'
Cuts at: 1137 1391 1137
Size: 254 3894
DraI TTT'AAA
Cuts at: 171 1147 171
Size: 976 3172
DrdI GACnn_nn'nnGTC
Cuts at: 1854 2712 1854
Size: 858 3290
EaeI y'GGCC_r
Cuts at: 185 260 383 509 1028 1134 1733 1907 2298
Size: 75 123 126 519 106 599 174 391
Cuts at: 2298 2325 3670 185
Size: 27 1345 663
Fragments arranged by size:
1345 663 599 519 391 174 126 123
106 75 27
EagI C'GGCC_G
Cuts at: 509 1028 1733 509
Size: 519 705 2924
Fragments arranged by size:
2924 705 519
EarI CTCTTCn'nnn_
Cuts at: 475 2171 2381 475
Size: 1696 210 2242
Fragments arranged by size:
2242 1696 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 220 398 646 2356 2666 2812 3958 220
Size: 178 248 1710 310 146 1146 410
Fragments arranged by size:
1710 1146 410 310 248 178 146
EcoICRI GAG'CTC
Cuts at: 624 1023 1077 1160 624
Size: 399 54 83 3612
Fragments arranged by size:
3612 399 83 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 364 1018 1039 1973 2405 3152 3420 315
Size: 49 654 21 934 432 747 268 1043
Fragments arranged by size:
1043 934 747 654 432 268 49 21
EcoO109I rG'GnC_Cy
Cuts at: 129 366 1014 1287 129
Size: 237 648 273 2990
Fragments arranged by size:
2990 648 273 237
EcoRI G'AATT_C
Cuts at: 1152 1152
Size: 4148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 4148
FspI TGC'GCA
Cuts at: 964 991 1929 964
Size: 27 938 3183
Fragments arranged by size:
3183 938 27
HaeII r_GCGC'y
Cuts at: 253 376 540 685 838 874 1582 1830 2852
Size: 123 164 145 153 36 708 248 1022
Cuts at: 2852 3831 253
Size: 979 570
Fragments arranged by size:
1022 979 708 570 248 164 153 145
123 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 445 457 477 489 1051 1083 1990 2020 2022
Size: 12 20 12 562 32 907 30 2
Cuts at: 2022 2052 445
Size: 30 2541
Fragments arranged by size:
2541 907 562 32 30 30 20 12
12 2
HincII GTy'rAC
Cuts at: 1187 1187
Size: 4148
KasI G'GCGC_C
Cuts at: 249 372 536 681 834 870 1578 1826 249
Size: 123 164 145 153 36 708 248 2571
Fragments arranged by size:
2571 708 248 164 153 145 123 36
KpnI G_GTAC'C
Cuts at: 1168 1168
Size: 4148
MluI A'CGCG_T
Cuts at: 3581 3581
Size: 4148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 488 500 2819 3003 488
Size: 12 2319 184 1633
Fragments arranged by size:
2319 1633 184 12
MscI TGG'CCA
Cuts at: 187 1909 187
Size: 1722 2426
MslI CAynn'nnrTG
Cuts at: 181 958 2137 2264 181
Size: 777 1179 127 2065
Fragments arranged by size:
2065 1179 777 127
MspA1I CmG'CkG
Cuts at: 247 259 351 652 770 823 1065 1107 1259
Size: 12 92 301 118 53 242 42 152
Cuts at: 1259 1573 1933 2946 3191 247
Size: 314 360 1013 245 1204
Fragments arranged by size:
1204 1013 360 314 301 245 242 152
118 92 53 42 12
NaeI GCC'GGC
Cuts at: 288 509 869 2329 288
Size: 221 360 1460 2107
Fragments arranged by size:
2107 1460 360 221
NarI GG'CG_CC
Cuts at: 250 373 537 682 835 871 1579 1827 250
Size: 123 164 145 153 36 708 248 2571
Fragments arranged by size:
2571 708 248 164 153 145 123 36
NcoI C'CATG_G
Cuts at: 176 176
Size: 4148
NgoMIV G'CCGG_C
Cuts at: 286 507 867 2327 286
Size: 221 360 1460 2107
Fragments arranged by size:
2107 1460 360 221
NspI r_CATG'y
Cuts at: 140 1201 1701 2608 140
Size: 1061 500 907 1680
Fragments arranged by size:
1680 1061 907 500
PciI A'CATG_T
Cuts at: 2604 2604
Size: 4148
PfoI T'CCnGG_A
Cuts at: 1016 1016
Size: 4148
PmeI GTTT'AAAC
Cuts at: 1147 1147
Size: 4148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 4116
PpuMI rG'GwC_Cy
Cuts at: 129 1014 129
Size: 885 3263
PstI C_TGCA'G
Cuts at: 1049 1195 1880 1049
Size: 146 685 3317
Fragments arranged by size:
3317 685 146
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4148
PvuII CAG'CTG
Cuts at: 1065 1107 1573 1933 1065
Size: 42 466 360 3280
Fragments arranged by size:
3280 466 360 42
RsrII CG'GwC_CG
Cuts at: 2343 2343
Size: 4148
SacI G_AGCT'C
Cuts at: 626 1025 1079 1162 626
Size: 399 54 83 3612
Fragments arranged by size:
3612 399 83 54
SacII CC_GC'GG
Cuts at: 248 260 653 248
Size: 12 393 3743
Fragments arranged by size:
3743 393 12
SalI G'TCGA_C
Cuts at: 1185 1185
Size: 4148
SanDI GG'GwC_CC
Cuts at: 129 129
Size: 4148
SapI GCTCTTCn'nnn_
Cuts at: 2171 2381 2171
Size: 210 3938
SbfI CC_TGCA'GG
Cuts at: 1195 1195
Size: 4148
ScaI AGT'ACT
Cuts at: 3665 3665
Size: 4148
SfcI C'TryA_G
Cuts at: 33 1045 1191 1876 2869 3060 33
Size: 1012 146 685 993 191 1121
Fragments arranged by size:
1121 1012 993 685 191 146
SfoI GGC'GCC
Cuts at: 251 374 538 683 836 872 1580 1828 251
Size: 123 164 145 153 36 708 248 2571
Fragments arranged by size:
2571 708 248 164 153 145 123 36
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
SgrAI Cr'CCGG_yG
Cuts at: 286 867 286
Size: 581 3567
SmaI CCC'GGG
Cuts at: 270 1170 270
Size: 900 3248
SmlI C'TyrA_G
Cuts at: 625 1303 2218 2710 2972 625
Size: 678 915 492 262 1801
Fragments arranged by size:
1801 915 678 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4148
SphI G_CATG'C
Cuts at: 1201 1701 1201
Size: 500 3648
StyI C'CwwG_G
Cuts at: 176 551 830 1282 1636 176
Size: 375 279 452 354 2688
Fragments arranged by size:
2688 452 375 354 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 605 1731 1864 2323 2532 605
Size: 1126 133 459 209 2221
Fragments arranged by size:
2221 1126 459 209 133
TatI w'GTAC_w
Cuts at: 926 3663 926
Size: 2737 1411
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 4116
Tth111I GACn'n_nGTC
Cuts at: 1945 1945
Size: 4148
XbaI T'CTAG_A
Cuts at: 1179 1179
Size: 4148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 903 1643 114
Size: 789 740 2619
Fragments arranged by size:
2619 789 740
XmaI C'CCGG_G
Cuts at: 268 1168 268
Size: 900 3248
XmnI GAAnn'nnTTC
Cuts at: 1096 1096
Size: 4148
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsmI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SexAI SfiI SnaBI SrfI
SspI StuI SwaI XhoI ZraI