Restriction map for
pF1KB9592
(Circular) (Six-base) MAPSORT of: pF1KB9592.seq Check: 1028 from: 1 to: 4892
pF1KB9592 4892 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 1496 1656 1930 1496
Size: 160 274 4458
Fragments arranged by size:
4458 274 160
Acc65I G'GTAC_C
Cuts at: 1908 1908
Size: 4892
AclI AA'CG_TT
Cuts at: 4732 4732
Size: 4892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1090 1904 2717 3149 3896 4164 1090
Size: 814 813 432 747 268 1818
Fragments arranged by size:
1818 814 813 747 432 268
AflIII A'CryG_T
Cuts at: 1659 1854 2244 3348 4325 1659
Size: 195 390 1104 977 2226
Fragments arranged by size:
2226 1104 977 390 195
AgeI A'CCGG_T
Cuts at: 767 3280 767
Size: 2513 2379
AhdI GACnn_n'nnGTC
Cuts at: 1688 1688
Size: 4892
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 305 305
Size: 4892
AlwNI CAG_nnn'CTG
Cuts at: 214 1428 1705 2197 3764 214
Size: 1214 277 492 1567 1342
Fragments arranged by size:
1567 1342 1214 492 277
ApaLI G'TGCA_C
Cuts at: 3662 3662
Size: 4892
ApoI r'AATT_y
Cuts at: 434 539 643 813 832 883 919 1896 4245
Size: 105 104 170 19 51 36 977 2349
Cuts at: 4245 4501 434
Size: 256 825
Fragments arranged by size:
2349 977 825 256 170 105 104 51
36 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 4892
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
AvaI C'yCGr_G
Cuts at: 1912 1912
Size: 4892
BamHI G'GATC_C
Cuts at: 819 1917 819
Size: 1098 3794
BanI G'GyrC_C
Cuts at: 1006 1908 2322 2570 2605 1006
Size: 902 414 248 35 3293
Fragments arranged by size:
3293 902 414 248 35
BanII G_rGCy'C
Cuts at: 1906 2936 1906
Size: 1030 3862
BbeI G_GCGC'C
Cuts at: 2326 2574 2326
Size: 248 4644
BbsI GAAGACnn'nnnn_
Cuts at: 1725 1725
Size: 4892
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4858
BciVI GTATCCnnnnn_n'
Cuts at: 1399 2790 3551 4892 1399
Size: 1391 761 1341 1399
Fragments arranged by size:
1399 1391 1341 761
BclI T'GATC_A
Cuts at: 1463 2412 1463
Size: 949 3943
BfrBI ATG'CAT
Cuts at: 878 878
Size: 4892
BglII A'GATC_T
Cuts at: 2407 4444 2407
Size: 2037 2855
BlpI GC'TnA_GC
Cuts at: 2004 2004
Size: 4892
Bme1580I G_kGCm'C
Cuts at: 2517 2610 3666 2517
Size: 93 1056 3743
Fragments arranged by size:
3743 1056 93
BmgBI CAC'GTC
Cuts at: 763 1660 763
Size: 897 3995
BmrI ACTGGGnnnn_n'
Cuts at: 2104 2185 2520 4664 2104
Size: 81 335 2144 2332
Fragments arranged by size:
2332 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 345 3259 345
Size: 2914 1978
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1666 1682 1699 1666
Size: 16 17 4859
Fragments arranged by size:
4859 17 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2068 2947 3439 3737 2068
Size: 879 492 298 3223
Fragments arranged by size:
3223 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1675 4596 1675
Size: 2921 1971
BsaAI yAC'GTr
Cuts at: 2245 2245
Size: 4892
BsaBI GATnn'nnATC
Cuts at: 6 1523 2406 4443 6
Size: 1517 883 2037 455
Fragments arranged by size:
2037 1517 883 455
BsaHI Gr'CG_yC
Cuts at: 2323 2571 3273 4769 2323
Size: 248 702 1496 2446
Fragments arranged by size:
2446 1496 702 248
BsaWI w'CCGG_w
Cuts at: 767 2081 2602 3280 3554 3701 4112 767
Size: 1314 521 678 274 147 411 1547
Fragments arranged by size:
1547 1314 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 432 432
Size: 4892
BseYI C'CCAG_C
Cuts at: 2317 3075 3652 4148 2317
Size: 758 577 496 3061
Fragments arranged by size:
3061 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 415
Size: 4892
BsiEI CG_ry'CG
Cuts at: 104 2434 2480 3688 104
Size: 2330 46 1208 1308
Fragments arranged by size:
2330 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1906 2684 2874 3666 1906
Size: 778 190 792 3132
Fragments arranged by size:
3132 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 753 753
Size: 4892
Bsp1286I G_dGCh'C
Cuts at: 1906 2517 2610 2684 2874 2936 3666 1906
Size: 611 93 74 190 62 730 3132
Fragments arranged by size:
3132 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4112 4112
Size: 4892
BspHI T'CATG_A
Cuts at: 4068 4068
Size: 4892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 472 1942 2156 2458 2839 472
Size: 1470 214 302 381 2525
Fragments arranged by size:
2525 1470 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3184 3238 4722 4887 50
Size: 3134 54 1484 165 55
Fragments arranged by size:
3134 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2804 2804
Size: 4892
BsrFI r'CCGG_y
Cuts at: 767 2890 3071 3280 767
Size: 2123 181 209 2379
Fragments arranged by size:
2379 2123 209 181
BsrGI T'GTAC_A
Cuts at: 468 618 468
Size: 150 4742
BssSI C'ACGA_G
Cuts at: 3163 3521 3163
Size: 358 4534
BstAPI GCAn_nnn'nTGC
Cuts at: 2269 2269
Size: 4892
BstBI TT'CG_AA
Cuts at: 1580 3253 1580
Size: 1673 3219
BstXI CCAn_nnnn'nTGG
Cuts at: 1017 1017
Size: 4892
BstYI r'GATC_y
Cuts at: 1 819 1917 2384 2407 2742 2988 4000 4086
Size: 818 1098 467 23 335 246 1012 86
Cuts at: 4086 4098 4444 1
Size: 12 346 449
Fragments arranged by size:
1098 1012 818 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 1539 1686 1539
Size: 147 4745
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1809 2842 3023 3186 1809
Size: 1033 181 163 3515
Fragments arranged by size:
3515 1033 181 163
BtsI GCAGTG_nn'
Cuts at: 2135 2135
Size: 4892
DraI TTT'AAA
Cuts at: 1280 1891 1280
Size: 611 4281
DrdI GACnn_nn'nnGTC
Cuts at: 2598 3456 2598
Size: 858 4034
EaeI y'GGCC_r
Cuts at: 1707 2477 2651 3042 3069 4414 1707
Size: 770 174 391 27 1345 2185
Fragments arranged by size:
2185 1345 770 391 174 27
EagI C'GGCC_G
Cuts at: 2477 2477
Size: 4892
EarI CTCTTCn'nnn_
Cuts at: 2915 3125 2915
Size: 210 4682
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3100 3410 3556 4702 3100
Size: 310 146 1146 3290
Fragments arranged by size:
3290 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1904 1904
Size: 4892
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1090 1666 1682 1699 1904 2717 3149 3896 4164
Size: 576 16 17 205 813 432 747 268
Cuts at: 4164 1090
Size: 1818
Fragments arranged by size:
1818 813 747 576 432 268 205 17
16
EcoO109I rG'GnC_Cy
Cuts at: 2031 2031
Size: 4892
EcoRI G'AATT_C
Cuts at: 643 919 1896 643
Size: 276 977 3639
Fragments arranged by size:
3639 977 276
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2911 2911
Size: 4892
FspI TGC'GCA
Cuts at: 2673 2673
Size: 4892
HaeII r_GCGC'y
Cuts at: 2326 2574 3596 4575 2326
Size: 248 1022 979 2643
Fragments arranged by size:
2643 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 214 246 307 325 339 357 1224 1256 1674
Size: 32 61 18 14 18 867 32 418
Cuts at: 1674 1706 2734 2764 2766 2796 214
Size: 32 1028 30 2 30 2310
Fragments arranged by size:
2310 1028 867 418 61 32 32 32
30 30 18 18 14 2
HincII GTy'rAC
Cuts at: 1657 1931 1657
Size: 274 4618
KasI G'GCGC_C
Cuts at: 2322 2570 2322
Size: 248 4644
KpnI G_GTAC'C
Cuts at: 1912 1912
Size: 4892
MfeI C'AATT_G
Cuts at: 1606 1606
Size: 4892
MluI A'CGCG_T
Cuts at: 4325 4325
Size: 4892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 617 3563 3747 617
Size: 2946 184 1762
Fragments arranged by size:
2946 1762 184
MscI TGG'CCA
Cuts at: 1709 2653 1709
Size: 944 3948
MslI CAynn'nnrTG
Cuts at: 520 2881 3008 520
Size: 2361 127 2404
Fragments arranged by size:
2404 2361 127
MspA1I CmG'CkG
Cuts at: 289 2003 2317 2677 3690 3935 289
Size: 1714 314 360 1013 245 1246
Fragments arranged by size:
1714 1246 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3073 3073
Size: 4892
NarI GG'CG_CC
Cuts at: 2323 2571 2323
Size: 248 4644
NcoI C'CATG_G
Cuts at: 1686 1686
Size: 4892
NdeI CA'TA_TG
Cuts at: 1620 1620
Size: 4892
NgoMIV G'CCGG_C
Cuts at: 3071 3071
Size: 4892
NsiI A_TGCA'T
Cuts at: 880 880
Size: 4892
NspI r_CATG'y
Cuts at: 799 1858 1945 2445 3352 799
Size: 1059 87 500 907 2339
Fragments arranged by size:
2339 1059 907 500 87
PciI A'CATG_T
Cuts at: 1854 3348 1854
Size: 1494 3398
PflMI CCAn_nnn'nTGG
Cuts at: 1485 1677 1705 1485
Size: 192 28 4672
Fragments arranged by size:
4672 192 28
PmeI GTTT'AAAC
Cuts at: 1891 1891
Size: 4892
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 4031
Size: 32 4860
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1465 1497 1465
Size: 32 4860
PstI C_TGCA'G
Cuts at: 1342 1939 2624 1342
Size: 597 685 3610
Fragments arranged by size:
3610 685 597
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4892
PvuII CAG'CTG
Cuts at: 289 2317 2677 289
Size: 2028 360 2504
Fragments arranged by size:
2504 2028 360
RsrII CG'GwC_CG
Cuts at: 3087 3087
Size: 4892
SacI G_AGCT'C
Cuts at: 1906 1906
Size: 4892
SalI G'TCGA_C
Cuts at: 1655 1929 1655
Size: 274 4618
SapI GCTCTTCn'nnn_
Cuts at: 2915 3125 2915
Size: 210 4682
SbfI CC_TGCA'GG
Cuts at: 1939 1939
Size: 4892
ScaI AGT'ACT
Cuts at: 589 4409 589
Size: 3820 1072
SfcI C'TryA_G
Cuts at: 33 179 206 1338 1935 2620 3613 3804 33
Size: 146 27 1132 597 685 993 191 1121
Fragments arranged by size:
1132 1121 993 685 597 191 146 27
SfoI GGC'GCC
Cuts at: 2324 2572 2324
Size: 248 4644
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
SmaI CCC'GGG
Cuts at: 1914 1914
Size: 4892
SmlI C'TyrA_G
Cuts at: 2047 2962 3454 3716 2047
Size: 915 492 262 3223
Fragments arranged by size:
3223 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4892
SphI G_CATG'C
Cuts at: 1945 2445 1945
Size: 500 4392
SspI AAT'ATT
Cuts at: 1332 1332
Size: 4892
StyI C'CwwG_G
Cuts at: 1686 2026 2380 1686
Size: 340 354 4198
Fragments arranged by size:
4198 354 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2475 2608 3067 3276 2475
Size: 133 459 209 4091
Fragments arranged by size:
4091 459 209 133
TatI w'GTAC_w
Cuts at: 468 587 618 738 4407 468
Size: 119 31 120 3669 953
Fragments arranged by size:
3669 953 120 119 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4409 4441 4409
Size: 32 4860
Tth111I GACn'n_nGTC
Cuts at: 565 2689 565
Size: 2124 2768
XbaI T'CTAG_A
Cuts at: 1923 1923
Size: 4892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2387 2387
Size: 4892
XmaI C'CCGG_G
Cuts at: 1912 1912
Size: 4892
XmnI GAAnn'nnTTC
Cuts at: 551 551
Size: 4892
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbvCI BglI BmtI Bpu10I BsaXI BsiWI
BsmI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NheI NotI
NruI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI