Restriction map for
pF1KB9603
(Circular) (Six-base) MAPSORT of: pF1KB9603.seq Check: 414 from: 1 to: 4148
pF1KB9603 4148 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1186 1186
Size: 4148
Acc65I G'GTAC_C
Cuts at: 1164 1164
Size: 4148
AclI AA'CG_TT
Cuts at: 3988 3988
Size: 4148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 873 920 1973 2405 3152 3420 873
Size: 47 1053 432 747 268 1601
Fragments arranged by size:
1601 1053 747 432 268 47
AflIII A'CryG_T
Cuts at: 500 1500 2604 3581 500
Size: 1000 1104 977 1067
Fragments arranged by size:
1104 1067 1000 977
AgeI A'CCGG_T
Cuts at: 2536 2536
Size: 4148
AlwNI CAG_nnn'CTG
Cuts at: 473 1453 3020 473
Size: 980 1567 1601
Fragments arranged by size:
1601 1567 980
ApaI G_GGCC'C
Cuts at: 553 561 649 685 974 553
Size: 8 88 36 289 3727
Fragments arranged by size:
3727 289 88 36 8
ApaLI G'TGCA_C
Cuts at: 2918 2918
Size: 4148
ApoI r'AATT_y
Cuts at: 1152 3501 3757 1152
Size: 2349 256 1543
Fragments arranged by size:
2349 1543 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
AvaI C'yCGr_G
Cuts at: 175 228 304 553 642 825 1081 1168 175
Size: 53 76 249 89 183 256 87 3155
Fragments arranged by size:
3155 256 249 183 89 87 76 53
AvrII C'CTAG_G
Cuts at: 951 951
Size: 4148
BamHI G'GATC_C
Cuts at: 1085 1173 1085
Size: 88 4060
BanI G'GyrC_C
Cuts at: 435 524 717 958 988 1164 1578 1826 1861
Size: 89 193 241 30 176 414 248 35
Cuts at: 1861 435
Size: 2722
Fragments arranged by size:
2722 414 248 241 193 176 89 35
30
BanII G_rGCy'C
Cuts at: 553 561 643 649 685 871 974 1162 2192
Size: 8 82 6 36 186 103 188 1030
Cuts at: 2192 553
Size: 2509
Fragments arranged by size:
2509 1030 188 186 103 82 36 8
6
BbeI G_GCGC'C
Cuts at: 528 721 1582 1830 528
Size: 193 861 248 2846
Fragments arranged by size:
2846 861 248 193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 623 657 623
Size: 34 4114
BciVI GTATCCnnnnn_n'
Cuts at: 2046 2807 4148 2046
Size: 761 1341 2046
Fragments arranged by size:
2046 1341 761
BclI T'GATC_A
Cuts at: 1668 1668
Size: 4148
BglI GCCn_nnn'nGGC
Cuts at: 211 565 211
Size: 354 3794
BglII A'GATC_T
Cuts at: 1663 3700 1663
Size: 2037 2111
BlpI GC'TnA_GC
Cuts at: 1260 1260
Size: 4148
Bme1580I G_kGCm'C
Cuts at: 440 553 561 649 685 974 1773 1866 2922
Size: 113 8 88 36 289 799 93 1056
Cuts at: 2922 440
Size: 1666
Fragments arranged by size:
1666 1056 799 289 113 93 88 36
8
BmrI ACTGGGnnnn_n'
Cuts at: 1360 1441 1776 3920 1360
Size: 81 335 2144 1588
Fragments arranged by size:
2144 1588 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 996 996
Size: 4148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 2203 2695 2993 1324
Size: 879 492 298 2479
Fragments arranged by size:
2479 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 174 235 3852 174
Size: 61 3617 470
Fragments arranged by size:
3617 470 61
BsaAI yAC'GTr
Cuts at: 1501 1501
Size: 4148
BsaBI GATnn'nnATC
Cuts at: 6 1662 3699 6
Size: 1656 2037 455
Fragments arranged by size:
2037 1656 455
BsaHI Gr'CG_yC
Cuts at: 525 718 1579 1827 2529 4025 525
Size: 193 861 248 702 1496 648
Fragments arranged by size:
1496 861 702 648 248 193
BsaWI w'CCGG_w
Cuts at: 1337 1858 2536 2810 2957 3368 1337
Size: 521 678 274 147 411 2117
Fragments arranged by size:
2117 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 173 203 173
Size: 30 4118
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 999 999
Size: 4148
BseYI C'CCAG_C
Cuts at: 1573 2331 2908 3404 1573
Size: 758 577 496 2317
Fragments arranged by size:
2317 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 457 788 797 981 1690 1736 2944 104
Size: 353 331 9 184 709 46 1208 1308
Fragments arranged by size:
1308 1208 709 353 331 184 46 9
BsiHKAI G_wGCw'C
Cuts at: 871 1162 1940 2130 2922 871
Size: 291 778 190 792 2097
Fragments arranged by size:
2097 792 778 291 190
BsmBI CGTCTCn'nnnn_
Cuts at: 580 580
Size: 4148
Bsp1286I G_dGCh'C
Cuts at: 440 553 561 643 649 685 871 974 1162
Size: 113 8 82 6 36 186 103 188
Cuts at: 1162 1773 1866 1940 2130 2192 2922 440
Size: 611 93 74 190 62 730 1666
Fragments arranged by size:
1666 730 611 190 188 186 113 103
93 82 74 62 36 8 6
BspEI T'CCGG_A
Cuts at: 3368 3368
Size: 4148
BspHI T'CATG_A
Cuts at: 3324 3324
Size: 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1104 1198 1412 1714 2095 1104
Size: 94 214 302 381 3157
Fragments arranged by size:
3157 381 302 214 94
BsrBI CCG'CTC
Cuts at: 50 629 2440 2494 3978 4143 50
Size: 579 1811 54 1484 165 55
Fragments arranged by size:
1811 1484 579 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2060 2060
Size: 4148
BsrFI r'CCGG_y
Cuts at: 374 521 714 2146 2327 2536 374
Size: 147 193 1432 181 209 1986
Fragments arranged by size:
1986 1432 209 193 181 147
BssHII G'CGCG_C
Cuts at: 808 808
Size: 4148
BssSI C'ACGA_G
Cuts at: 2419 2777 2419
Size: 358 3790
BstAPI GCAn_nnn'nTGC
Cuts at: 1525 1525
Size: 4148
BstBI TT'CG_AA
Cuts at: 2509 2509
Size: 4148
BstYI r'GATC_y
Cuts at: 1 1085 1173 1640 1663 1998 2244 3256 3342
Size: 1084 88 467 23 335 246 1012 86
Cuts at: 3342 3354 3700 1
Size: 12 346 449
Fragments arranged by size:
1084 1012 467 449 346 335 246 88
86 23 12
BtgI C'CryG_G
Cuts at: 209 284 677 788 209
Size: 75 393 111 3569
Fragments arranged by size:
3569 393 111 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 255 264 2098 2279 2442 255
Size: 9 1834 181 163 1961
Fragments arranged by size:
1961 1834 181 163 9
BtsI GCAGTG_nn'
Cuts at: 1391 1391
Size: 4148
DraI TTT'AAA
Cuts at: 1147 1147
Size: 4148
DrdI GACnn_nn'nnGTC
Cuts at: 427 1057 1854 2712 427
Size: 630 797 858 1863
Fragments arranged by size:
1863 858 797 630
EaeI y'GGCC_r
Cuts at: 143 454 730 785 798 978 1001 1733 1907
Size: 311 276 55 13 180 23 732 174
Cuts at: 1907 2298 2325 3670 143
Size: 391 27 1345 621
Fragments arranged by size:
1345 732 621 391 311 276 180 174
55 27 23 13
EagI C'GGCC_G
Cuts at: 454 785 978 1733 454
Size: 331 193 755 2869
Fragments arranged by size:
2869 755 331 193
EarI CTCTTCn'nnn_
Cuts at: 124 526 859 2171 2381 124
Size: 402 333 1312 210 1891
Fragments arranged by size:
1891 1312 402 333 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 388 701 1013 2356 2666 2812 3958 388
Size: 313 312 1343 310 146 1146 578
Fragments arranged by size:
1343 1146 578 313 312 310 146
EcoICRI GAG'CTC
Cuts at: 869 1160 869
Size: 291 3857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 873 920 996 1973 2405 3152 3420 873
Size: 47 76 977 432 747 268 1601
Fragments arranged by size:
1601 977 747 432 268 76 47
EcoO109I rG'GnC_Cy
Cuts at: 550 557 558 575 646 682 830 1287 550
Size: 7 1 17 71 36 148 457 3411
Fragments arranged by size:
3411 457 148 71 36 17 7 1
EcoRI G'AATT_C
Cuts at: 1152 1152
Size: 4148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 4148
FspI TGC'GCA
Cuts at: 1929 1929
Size: 4148
HaeII r_GCGC'y
Cuts at: 528 676 721 1582 1830 2852 3831 528
Size: 148 45 861 248 1022 979 845
Fragments arranged by size:
1022 979 861 845 248 148 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 173 205 691 723 1990 2020 2022 2052 173
Size: 32 486 32 1267 30 2 30 2269
Fragments arranged by size:
2269 1267 486 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1187 1187
Size: 4148
KasI G'GCGC_C
Cuts at: 524 717 1578 1826 524
Size: 193 861 248 2846
Fragments arranged by size:
2846 861 248 193
KpnI G_GTAC'C
Cuts at: 1168 1168
Size: 4148
MluI A'CGCG_T
Cuts at: 3581 3581
Size: 4148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 896 2819 3003 896
Size: 1923 184 2041
Fragments arranged by size:
2041 1923 184
MscI TGG'CCA
Cuts at: 1909 1909
Size: 4148
MslI CAynn'nnrTG
Cuts at: 283 2137 2264 283
Size: 1854 127 2167
Fragments arranged by size:
2167 1854 127
MspA1I CmG'CkG
Cuts at: 211 491 679 784 1259 1573 1933 2946 3191
Size: 280 188 105 475 314 360 1013 245
Cuts at: 3191 211
Size: 1168
Fragments arranged by size:
1168 1013 475 360 314 280 245 188
105
NaeI GCC'GGC
Cuts at: 716 2329 716
Size: 1613 2535
NarI GG'CG_CC
Cuts at: 525 718 1579 1827 525
Size: 193 861 248 2846
Fragments arranged by size:
2846 861 248 193
NcoI C'CATG_G
Cuts at: 284 284
Size: 4148
NgoMIV G'CCGG_C
Cuts at: 714 2327 714
Size: 1613 2535
NspI r_CATG'y
Cuts at: 504 1060 1201 1701 2608 504
Size: 556 141 500 907 2044
Fragments arranged by size:
2044 907 556 500 141
PasI CC'CwG_GG
Cuts at: 774 774
Size: 4148
PciI A'CATG_T
Cuts at: 500 2604 500
Size: 2104 2044
PfoI T'CCnGG_A
Cuts at: 293 695 293
Size: 402 3746
PmeI GTTT'AAAC
Cuts at: 1147 1147
Size: 4148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 4116
PpuMI rG'GwC_Cy
Cuts at: 830 830
Size: 4148
PspOMI G'GGCC_C
Cuts at: 549 557 645 681 970 549
Size: 8 88 36 289 3727
Fragments arranged by size:
3727 289 88 36 8
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 502 534 502
Size: 32 4116
PstI C_TGCA'G
Cuts at: 668 1115 1195 1880 668
Size: 447 80 685 2936
Fragments arranged by size:
2936 685 447 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4148
PvuII CAG'CTG
Cuts at: 1573 1933 1573
Size: 360 3788
RsrII CG'GwC_CG
Cuts at: 2343 2343
Size: 4148
SacI G_AGCT'C
Cuts at: 871 1162 871
Size: 291 3857
SacII CC_GC'GG
Cuts at: 212 680 212
Size: 468 3680
SalI G'TCGA_C
Cuts at: 1185 1185
Size: 4148
SanDI GG'GwC_CC
Cuts at: 830 830
Size: 4148
SapI GCTCTTCn'nnn_
Cuts at: 859 2171 2381 859
Size: 1312 210 2626
Fragments arranged by size:
2626 1312 210
SbfI CC_TGCA'GG
Cuts at: 668 1115 1195 668
Size: 447 80 3621
Fragments arranged by size:
3621 447 80
ScaI AGT'ACT
Cuts at: 3665 3665
Size: 4148
SfcI C'TryA_G
Cuts at: 33 261 664 1111 1191 1876 2869 3060 33
Size: 228 403 447 80 685 993 191 1121
Fragments arranged by size:
1121 993 685 447 403 228 191 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 565 565
Size: 4148
SfoI GGC'GCC
Cuts at: 526 719 1580 1828 526
Size: 193 861 248 2846
Fragments arranged by size:
2846 861 248 193
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
SgrAI Cr'CCGG_yG
Cuts at: 714 714
Size: 4148
SmaI CCC'GGG
Cuts at: 555 827 1170 555
Size: 272 343 3533
Fragments arranged by size:
3533 343 272
SmlI C'TyrA_G
Cuts at: 1303 2218 2710 2972 1303
Size: 915 492 262 2479
Fragments arranged by size:
2479 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4148
SphI G_CATG'C
Cuts at: 1201 1701 1201
Size: 500 3648
StyI C'CwwG_G
Cuts at: 284 951 1282 1636 284
Size: 667 331 354 2796
Fragments arranged by size:
2796 667 354 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 976 1731 1864 2323 2532 976
Size: 755 133 459 209 2592
Fragments arranged by size:
2592 755 459 209 133
TatI w'GTAC_w
Cuts at: 3663 3663
Size: 4148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 4116
Tth111I GACn'n_nGTC
Cuts at: 741 1945 741
Size: 1204 2944
XbaI T'CTAG_A
Cuts at: 1179 1179
Size: 4148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1643 1643
Size: 4148
XmaI C'CCGG_G
Cuts at: 553 825 1168 553
Size: 272 343 3533
Fragments arranged by size:
3533 343 272
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsrGI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PshAI PsiI PspXI SexAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI