Restriction map for
pF1KB9606
(Circular) (Six-base) MAPSORT of: pF1KB9606.seq Check: 7740 from: 1 to: 4190
pF1KB9606 4190 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1228 1228
Size: 4190
Acc65I G'GTAC_C
Cuts at: 926 1206 926
Size: 280 3910
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2447 3194 3462 2015
Size: 432 747 268 2743
Fragments arranged by size:
2743 747 432 268
AflIII A'CryG_T
Cuts at: 1542 2646 3623 1542
Size: 1104 977 2109
Fragments arranged by size:
2109 1104 977
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AhdI GACnn_n'nnGTC
Cuts at: 396 396
Size: 4190
AlwNI CAG_nnn'CTG
Cuts at: 169 1125 1495 3062 169
Size: 956 370 1567 1297
Fragments arranged by size:
1567 1297 956 370
ApaLI G'TGCA_C
Cuts at: 2960 2960
Size: 4190
ApoI r'AATT_y
Cuts at: 1194 3543 3799 1194
Size: 2349 256 1585
Fragments arranged by size:
2349 1585 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 229 445 1210 229
Size: 216 765 3209
Fragments arranged by size:
3209 765 216
BamHI G'GATC_C
Cuts at: 671 1215 671
Size: 544 3646
BanI G'GyrC_C
Cuts at: 225 926 1206 1620 1868 1903 225
Size: 701 280 414 248 35 2512
Fragments arranged by size:
2512 701 414 280 248 35
BanII G_rGCy'C
Cuts at: 504 968 1204 2234 504
Size: 464 236 1030 2460
Fragments arranged by size:
2460 1030 464 236
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3942
BciVI GTATCCnnnnn_n'
Cuts at: 2088 2849 4190 2088
Size: 761 1341 2088
Fragments arranged by size:
2088 1341 761
BclI T'GATC_A
Cuts at: 741 1710 741
Size: 969 3221
BglI GCCn_nnn'nGGC
Cuts at: 209 209
Size: 4190
BglII A'GATC_T
Cuts at: 1705 3742 1705
Size: 2037 2153
BlpI GC'TnA_GC
Cuts at: 1302 1302
Size: 4190
Bme1580I G_kGCm'C
Cuts at: 230 1815 1908 2964 230
Size: 1585 93 1056 1456
Fragments arranged by size:
1585 1456 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 261 1402 1483 1818 3962 261
Size: 1141 81 335 2144 489
Fragments arranged by size:
2144 1141 489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1057 1102 1108 1009
Size: 48 45 6 4091
Fragments arranged by size:
4091 48 45 6
Bpu10I CC'TnA_GC
Cuts at: 197 197
Size: 4190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 2245 2737 3035 1366
Size: 879 492 298 2521
Fragments arranged by size:
2521 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3894 3894
Size: 4190
BsaAI yAC'GTr
Cuts at: 1543 1543
Size: 4190
BsaBI GATnn'nnATC
Cuts at: 6 1704 3741 6
Size: 1698 2037 455
Fragments arranged by size:
2037 1698 455
BsaHI Gr'CG_yC
Cuts at: 442 573 1621 1869 2571 4067 442
Size: 131 1048 248 702 1496 565
Fragments arranged by size:
1496 1048 702 565 248 131
BsaWI w'CCGG_w
Cuts at: 1379 1900 2578 2852 2999 3410 1379
Size: 521 678 274 147 411 2159
Fragments arranged by size:
2159 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 247 538 769 862 232
Size: 15 291 231 93 3560
Fragments arranged by size:
3560 291 231 93 15
BseYI C'CCAG_C
Cuts at: 464 549 1050 1615 2373 2950 3446 464
Size: 85 501 565 758 577 496 1208
Fragments arranged by size:
1208 758 577 565 501 496 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 1126
Size: 4190
BsiEI CG_ry'CG
Cuts at: 104 707 1732 1778 2986 104
Size: 603 1025 46 1208 1308
Fragments arranged by size:
1308 1208 1025 603 46
BsiHKAI G_wGCw'C
Cuts at: 968 1204 1982 2172 2964 968
Size: 236 778 190 792 2194
Fragments arranged by size:
2194 792 778 236 190
BsiWI C'GTAC_G
Cuts at: 917 917
Size: 4190
BsmBI CGTCTCn'nnnn_
Cuts at: 564 907 1175 564
Size: 343 268 3579
Fragments arranged by size:
3579 343 268
Bsp1286I G_dGCh'C
Cuts at: 230 504 968 1204 1815 1908 1982 2172 2234
Size: 274 464 236 611 93 74 190 62
Cuts at: 2234 2964 230
Size: 730 1456
Fragments arranged by size:
1456 730 611 464 274 236 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3410 3410
Size: 4190
BspHI T'CATG_A
Cuts at: 474 3366 474
Size: 2892 1298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1240 1454 1756 2137 1240
Size: 214 302 381 3293
Fragments arranged by size:
3293 381 302 214
BsrBI CCG'CTC
Cuts at: 50 222 924 2482 2536 4020 4185 50
Size: 172 702 1558 54 1484 165 55
Fragments arranged by size:
1558 1484 702 172 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2102 2102
Size: 4190
BsrFI r'CCGG_y
Cuts at: 423 702 772 1025 1097 1145 2188 2369 2578
Size: 279 70 253 72 48 1043 181 209
Cuts at: 2578 423
Size: 2035
Fragments arranged by size:
2035 1043 279 253 209 181 72 70
48
BsrGI T'GTAC_A
Cuts at: 160 160
Size: 4190
BssHII G'CGCG_C
Cuts at: 1104 1104
Size: 4190
BssSI C'ACGA_G
Cuts at: 2461 2819 2461
Size: 358 3832
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 1567
Size: 4190
BstBI TT'CG_AA
Cuts at: 2551 2551
Size: 4190
BstXI CCAn_nnnn'nTGG
Cuts at: 274 495 274
Size: 221 3969
BstYI r'GATC_y
Cuts at: 1 671 762 1215 1682 1705 2040 2286 3298
Size: 670 91 453 467 23 335 246 1012
Cuts at: 3298 3384 3396 3742 1
Size: 86 12 346 449
Fragments arranged by size:
1012 670 467 453 449 346 335 246
91 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 833 878 2140 2321 2484 833
Size: 45 1262 181 163 2539
Fragments arranged by size:
2539 1262 181 163 45
BtsI GCAGTG_nn'
Cuts at: 1433 1433
Size: 4190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 308 343 308
Size: 35 4155
DraI TTT'AAA
Cuts at: 1189 1189
Size: 4190
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2754 1896
Size: 858 3332
EaeI y'GGCC_r
Cuts at: 110 276 1775 1949 2340 2367 3712 110
Size: 166 1499 174 391 27 1345 588
Fragments arranged by size:
1499 1345 588 391 174 166 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 179 515 620 2213 2423 179
Size: 336 105 1593 210 1946
Fragments arranged by size:
1946 1593 336 210 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2398 2708 2854 4000 2398
Size: 310 146 1146 2588
Fragments arranged by size:
2588 1146 310 146
EcoICRI GAG'CTC
Cuts at: 966 1202 966
Size: 236 3954
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1057 1102 1108 2015 2447 3194 3462 1009
Size: 48 45 6 907 432 747 268 1737
Fragments arranged by size:
1737 907 747 432 268 48 45 6
EcoNI CCTnn'n_nnAGG
Cuts at: 364 509 364
Size: 145 4045
EcoO109I rG'GnC_Cy
Cuts at: 1329 1329
Size: 4190
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 4190
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4190
HaeII r_GCGC'y
Cuts at: 204 1165 1624 1872 2894 3873 204
Size: 961 459 248 1022 979 521
Fragments arranged by size:
1022 979 961 521 459 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 382 414 2032 2062 2064 2094 382
Size: 32 1618 30 2 30 2478
Fragments arranged by size:
2478 1618 32 30 30 2
HincII GTy'rAC
Cuts at: 1229 1229
Size: 4190
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3942
KpnI G_GTAC'C
Cuts at: 930 1210 930
Size: 280 3910
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 830 2861 3045 830
Size: 2031 184 1975
Fragments arranged by size:
2031 1975 184
MscI TGG'CCA
Cuts at: 1951 1951
Size: 4190
MslI CAynn'nnrTG
Cuts at: 2179 2306 2179
Size: 127 4063
MspA1I CmG'CkG
Cuts at: 403 549 900 1122 1301 1615 1975 2988 3233
Size: 146 351 222 179 314 360 1013 245
Cuts at: 3233 403
Size: 1360
Fragments arranged by size:
1360 1013 360 351 314 245 222 179
146
NaeI GCC'GGC
Cuts at: 425 2371 425
Size: 1946 2244
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3942
NcoI C'CATG_G
Cuts at: 107 107
Size: 4190
NgoMIV G'CCGG_C
Cuts at: 423 2369 423
Size: 1946 2244
NspI r_CATG'y
Cuts at: 1243 1743 2650 1243
Size: 500 907 2783
Fragments arranged by size:
2783 907 500
PciI A'CATG_T
Cuts at: 2646 2646
Size: 4190
PfoI T'CCnGG_A
Cuts at: 179 179
Size: 4190
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4158
PshAI GACnn'nnGTC
Cuts at: 576 576
Size: 4190
PstI C_TGCA'G
Cuts at: 899 947 1237 1922 899
Size: 48 290 685 3167
Fragments arranged by size:
3167 685 290 48
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 549 1122 1615 1975 549
Size: 573 493 360 2764
Fragments arranged by size:
2764 573 493 360
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 968 1204 968
Size: 236 3954
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SapI GCTCTTCn'nnn_
Cuts at: 179 2213 2423 179
Size: 2034 210 1946
Fragments arranged by size:
2034 1946 210
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 3707 3707
Size: 4190
SfcI C'TryA_G
Cuts at: 33 895 943 1233 1918 2911 3102 33
Size: 862 48 290 685 993 191 1121
Fragments arranged by size:
1121 993 862 685 290 191 48
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4190
SmlI C'TyrA_G
Cuts at: 1345 2260 2752 3014 1345
Size: 915 492 262 2521
Fragments arranged by size:
2521 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
StuI AGG'CCT
Cuts at: 340 340
Size: 4190
StyI C'CwwG_G
Cuts at: 107 692 1324 1678 107
Size: 585 632 354 2619
Fragments arranged by size:
2619 632 585 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 262 1042 1144 1773 1906 2365 2574 262
Size: 780 102 629 133 459 209 1878
Fragments arranged by size:
1878 780 629 459 209 133 102
TatI w'GTAC_w
Cuts at: 160 779 3705 160
Size: 619 2926 645
Fragments arranged by size:
2926 645 619
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 229 261 3707 3739 229
Size: 32 3446 32 680
Fragments arranged by size:
3446 680 32 32
Tth111I GACn'n_nGTC
Cuts at: 1987 1987
Size: 4190
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1685 1685
Size: 4190
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4190
XmnI GAAnn'nnTTC
Cuts at: 637 637
Size: 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BsaXI BsmI BstEII BstZ17I Bsu36I ClaI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI