Restriction map for
pF1KB9607
(Circular) (Six-base) MAPSORT of: pF1KB9607.seq Check: 5464 from: 1 to: 3704
pF1KB9607 3704 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 742 742
Size: 3704
Acc65I G'GTAC_C
Cuts at: 720 720
Size: 3704
AclI AA'CG_TT
Cuts at: 3544 3544
Size: 3704
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 1529 1961 2708 2976 397
Size: 1132 432 747 268 1125
Fragments arranged by size:
1132 1125 747 432 268
AflIII A'CryG_T
Cuts at: 401 1056 2160 3137 401
Size: 655 1104 977 968
Fragments arranged by size:
1104 977 968 655
AgeI A'CCGG_T
Cuts at: 2092 2092
Size: 3704
AhdI GACnn_n'nnGTC
Cuts at: 315 315
Size: 3704
AlwNI CAG_nnn'CTG
Cuts at: 141 205 1009 2576 141
Size: 64 804 1567 1269
Fragments arranged by size:
1567 1269 804 64
ApaI G_GGCC'C
Cuts at: 175 175
Size: 3704
ApaLI G'TGCA_C
Cuts at: 604 2474 604
Size: 1870 1834
ApoI r'AATT_y
Cuts at: 708 3057 3313 708
Size: 2349 256 1099
Fragments arranged by size:
2349 1099 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3704
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
AvaI C'yCGr_G
Cuts at: 724 724
Size: 3704
BamHI G'GATC_C
Cuts at: 729 729
Size: 3704
BanI G'GyrC_C
Cuts at: 503 720 1134 1382 1417 503
Size: 217 414 248 35 2790
Fragments arranged by size:
2790 414 248 217 35
BanII G_rGCy'C
Cuts at: 175 348 718 1748 175
Size: 173 370 1030 2131
Fragments arranged by size:
2131 1030 370 173
BbeI G_GCGC'C
Cuts at: 1138 1386 1138
Size: 248 3456
BbsI GAAGACnn'nnnn_
Cuts at: 384 384
Size: 3704
BbvCI CC'TCA_GC
Cuts at: 134 134
Size: 3704
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3670
BciVI GTATCCnnnnn_n'
Cuts at: 1602 2363 3704 1602
Size: 761 1341 1602
Fragments arranged by size:
1602 1341 761
BclI T'GATC_A
Cuts at: 1224 1224
Size: 3704
BglI GCCn_nnn'nGGC
Cuts at: 343 343
Size: 3704
BglII A'GATC_T
Cuts at: 1219 3256 1219
Size: 2037 1667
BlpI GC'TnA_GC
Cuts at: 816 816
Size: 3704
Bme1580I G_kGCm'C
Cuts at: 175 608 1329 1422 2478 175
Size: 433 721 93 1056 1401
Fragments arranged by size:
1401 1056 721 433 93
BmrI ACTGGGnnnn_n'
Cuts at: 916 997 1332 3476 916
Size: 81 335 2144 1144
Fragments arranged by size:
2144 1144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 3704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 332
Size: 3704
Bpu10I CC'TnA_GC
Cuts at: 134 134
Size: 3704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 880 1759 2251 2549 369
Size: 511 879 492 298 1524
Fragments arranged by size:
1524 879 511 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 154 3408 154
Size: 3254 450
BsaAI yAC'GTr
Cuts at: 1057 1057
Size: 3704
BsaBI GATnn'nnATC
Cuts at: 6 1218 3255 6
Size: 1212 2037 455
Fragments arranged by size:
2037 1212 455
BsaHI Gr'CG_yC
Cuts at: 1135 1383 2085 3581 1135
Size: 248 702 1496 1258
Fragments arranged by size:
1496 1258 702 248
BsaWI w'CCGG_w
Cuts at: 893 1414 2092 2366 2513 2924 893
Size: 521 678 274 147 411 1673
Fragments arranged by size:
1673 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 207 236 328 207
Size: 29 92 3583
Fragments arranged by size:
3583 92 29
BseYI C'CCAG_C
Cuts at: 590 1129 1887 2464 2960 590
Size: 539 758 577 496 1334
Fragments arranged by size:
1334 758 577 539 496
BsiEI CG_ry'CG
Cuts at: 104 1246 1292 2500 104
Size: 1142 46 1208 1308
Fragments arranged by size:
1308 1208 1142 46
BsiHKAI G_wGCw'C
Cuts at: 385 608 718 1496 1686 2478 385
Size: 223 110 778 190 792 1611
Fragments arranged by size:
1611 792 778 223 190 110
BsmI GAATG_Cn'
Cuts at: 475 475
Size: 3704
Bsp1286I G_dGCh'C
Cuts at: 175 348 385 608 718 1329 1422 1496 1686
Size: 173 37 223 110 611 93 74 190
Cuts at: 1686 1748 2478 175
Size: 62 730 1401
Fragments arranged by size:
1401 730 611 223 190 173 110 93
74 62 37
BspEI T'CCGG_A
Cuts at: 2924 2924
Size: 3704
BspHI T'CATG_A
Cuts at: 2880 2880
Size: 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 275 754 968 1270 1651 275
Size: 479 214 302 381 2328
Fragments arranged by size:
2328 479 381 302 214
BsrBI CCG'CTC
Cuts at: 50 194 552 1996 2050 3534 3699 50
Size: 144 358 1444 54 1484 165 55
Fragments arranged by size:
1484 1444 358 165 144 55 54
BsrDI GCAATG_nn'
Cuts at: 1616 1616
Size: 3704
BsrFI r'CCGG_y
Cuts at: 1702 1883 2092 1702
Size: 181 209 3314
Fragments arranged by size:
3314 209 181
BssHII G'CGCG_C
Cuts at: 483 483
Size: 3704
BssSI C'ACGA_G
Cuts at: 1975 2333 1975
Size: 358 3346
BstAPI GCAn_nnn'nTGC
Cuts at: 1081 1081
Size: 3704
BstBI TT'CG_AA
Cuts at: 2065 2065
Size: 3704
BstXI CCAn_nnnn'nTGG
Cuts at: 495 519 495
Size: 24 3680
BstYI r'GATC_y
Cuts at: 1 226 729 1196 1219 1554 1800 2812 2898
Size: 225 503 467 23 335 246 1012 86
Cuts at: 2898 2910 3256 1
Size: 12 346 449
Fragments arranged by size:
1012 503 467 449 346 335 246 225
86 23 12
BtgI C'CryG_G
Cuts at: 164 540 164
Size: 376 3328
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1654 1835 1998 1654
Size: 181 163 3360
Fragments arranged by size:
3360 181 163
BtsI GCAGTG_nn'
Cuts at: 636 667 947 636
Size: 31 280 3393
Fragments arranged by size:
3393 280 31
DraI TTT'AAA
Cuts at: 703 703
Size: 3704
DraIII CAC_nnn'GTG
Cuts at: 686 686
Size: 3704
DrdI GACnn_nn'nnGTC
Cuts at: 1410 2268 1410
Size: 858 2846
EaeI y'GGCC_r
Cuts at: 1289 1463 1854 1881 3226 1289
Size: 174 391 27 1345 1767
Fragments arranged by size:
1767 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1289 1289
Size: 3704
EarI CTCTTCn'nnn_
Cuts at: 1727 1937 1727
Size: 210 3494
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1912 2222 2368 3514 1912
Size: 310 146 1146 2102
Fragments arranged by size:
2102 1146 310 146
EcoICRI GAG'CTC
Cuts at: 716 716
Size: 3704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 397 1529 1961 2708 2976 332
Size: 65 1132 432 747 268 1060
Fragments arranged by size:
1132 1060 747 432 268 65
EcoO109I rG'GnC_Cy
Cuts at: 171 172 843 171
Size: 1 671 3032
Fragments arranged by size:
3032 671 1
EcoRI G'AATT_C
Cuts at: 708 708
Size: 3704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1723 1723
Size: 3704
FspI TGC'GCA
Cuts at: 1485 1485
Size: 3704
HaeII r_GCGC'y
Cuts at: 1138 1386 2408 3387 1138
Size: 248 1022 979 1455
Fragments arranged by size:
1455 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 301 333 388 410 420 442 1546 1576 1578
Size: 32 55 22 10 22 1104 30 2
Cuts at: 1578 1608 301
Size: 30 2397
Fragments arranged by size:
2397 1104 55 32 30 30 22 22
10 2
HincII GTy'rAC
Cuts at: 743 743
Size: 3704
KasI G'GCGC_C
Cuts at: 1134 1382 1134
Size: 248 3456
KpnI G_GTAC'C
Cuts at: 724 724
Size: 3704
MluI A'CGCG_T
Cuts at: 3137 3137
Size: 3704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 163 2375 2559 163
Size: 2212 184 1308
Fragments arranged by size:
2212 1308 184
MscI TGG'CCA
Cuts at: 1465 1465
Size: 3704
MslI CAynn'nnrTG
Cuts at: 112 1693 1820 112
Size: 1581 127 1996
Fragments arranged by size:
1996 1581 127
MspA1I CmG'CkG
Cuts at: 371 815 1129 1489 2502 2747 371
Size: 444 314 360 1013 245 1328
Fragments arranged by size:
1328 1013 444 360 314 245
NaeI GCC'GGC
Cuts at: 1885 1885
Size: 3704
NarI GG'CG_CC
Cuts at: 1135 1383 1135
Size: 248 3456
NcoI C'CATG_G
Cuts at: 164 164
Size: 3704
NgoMIV G'CCGG_C
Cuts at: 1883 1883
Size: 3704
NspI r_CATG'y
Cuts at: 405 757 1257 2164 405
Size: 352 500 907 1945
Fragments arranged by size:
1945 907 500 352
PciI A'CATG_T
Cuts at: 401 2160 401
Size: 1759 1945
PflMI CCAn_nnn'nTGG
Cuts at: 205 205
Size: 3704
PfoI T'CCnGG_A
Cuts at: 569 569
Size: 3704
PmeI GTTT'AAAC
Cuts at: 703 703
Size: 3704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2843 2875 2843
Size: 32 3672
PspOMI G'GGCC_C
Cuts at: 171 171
Size: 3704
PstI C_TGCA'G
Cuts at: 674 751 1436 674
Size: 77 685 2942
Fragments arranged by size:
2942 685 77
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3704
PvuII CAG'CTG
Cuts at: 371 1129 1489 371
Size: 758 360 2586
Fragments arranged by size:
2586 758 360
RsrII CG'GwC_CG
Cuts at: 1899 1899
Size: 3704
SacI G_AGCT'C
Cuts at: 718 718
Size: 3704
SalI G'TCGA_C
Cuts at: 741 741
Size: 3704
SapI GCTCTTCn'nnn_
Cuts at: 1727 1937 1727
Size: 210 3494
SbfI CC_TGCA'GG
Cuts at: 751 751
Size: 3704
ScaI AGT'ACT
Cuts at: 3221 3221
Size: 3704
SfcI C'TryA_G
Cuts at: 33 366 670 747 1432 2425 2616 33
Size: 333 304 77 685 993 191 1121
Fragments arranged by size:
1121 993 685 333 304 191 77
SfoI GGC'GCC
Cuts at: 1136 1384 1136
Size: 248 3456
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
SmaI CCC'GGG
Cuts at: 726 726
Size: 3704
SmlI C'TyrA_G
Cuts at: 384 859 1774 2266 2528 384
Size: 475 915 492 262 1560
Fragments arranged by size:
1560 915 492 475 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3704
SphI G_CATG'C
Cuts at: 757 1257 757
Size: 500 3204
StyI C'CwwG_G
Cuts at: 164 250 838 1192 164
Size: 86 588 354 2676
Fragments arranged by size:
2676 588 354 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 445 1287 1420 1879 2088 445
Size: 842 133 459 209 2061
Fragments arranged by size:
2061 842 459 209 133
TatI w'GTAC_w
Cuts at: 398 3219 398
Size: 2821 883
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 3672
Tth111I GACn'n_nGTC
Cuts at: 1501 1501
Size: 3704
XbaI T'CTAG_A
Cuts at: 735 735
Size: 3704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1199 1199
Size: 3704
XmaI C'CCGG_G
Cuts at: 724 724
Size: 3704
XmnI GAAnn'nnTTC
Cuts at: 475 475
Size: 3704
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI