Restriction map for
pF1KB9610
(Circular) (Six-base) MAPSORT of: pF1KB9610.seq Check: 7955 from: 1 to: 4682
pF1KB9610 4682 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 327 327
Size: 4682
AccI GT'mk_AC
Cuts at: 1720 1720
Size: 4682
Acc65I G'GTAC_C
Cuts at: 1161 1698 1161
Size: 537 4145
AclI AA'CG_TT
Cuts at: 4522 4522
Size: 4682
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2507 2939 3686 3954 2507
Size: 432 747 268 3235
Fragments arranged by size:
3235 747 432 268
AfeI AGC'GCT
Cuts at: 1220 1220
Size: 4682
AflIII A'CryG_T
Cuts at: 2034 3138 4115 2034
Size: 1104 977 2601
Fragments arranged by size:
2601 1104 977
AgeI A'CCGG_T
Cuts at: 3070 3070
Size: 4682
AleI CACnn'nnGTG
Cuts at: 1306 1306
Size: 4682
AlwNI CAG_nnn'CTG
Cuts at: 594 774 1485 1500 1570 1987 3554 594
Size: 180 711 15 70 417 1567 1722
Fragments arranged by size:
1722 1567 711 417 180 70 15
ApaI G_GGCC'C
Cuts at: 1254 1564 1668 1254
Size: 310 104 4268
Fragments arranged by size:
4268 310 104
ApaLI G'TGCA_C
Cuts at: 233 3452 233
Size: 3219 1463
ApoI r'AATT_y
Cuts at: 456 1686 4035 4291 456
Size: 1230 2349 256 847
Fragments arranged by size:
2349 1230 847 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4682
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4682
AvaI C'yCGr_G
Cuts at: 812 1361 1702 812
Size: 549 341 3792
Fragments arranged by size:
3792 549 341
BamHI G'GATC_C
Cuts at: 1707 1707
Size: 4682
BanI G'GyrC_C
Cuts at: 572 750 1161 1295 1698 2112 2360 2395 572
Size: 178 411 134 403 414 248 35 2859
Fragments arranged by size:
2859 414 411 403 248 178 134 35
BanII G_rGCy'C
Cuts at: 734 797 1157 1254 1494 1564 1577 1624 1631
Size: 63 360 97 240 70 13 47 7
Cuts at: 1631 1668 1696 2726 734
Size: 37 28 1030 2690
Fragments arranged by size:
2690 1030 360 240 97 70 63 47
37 28 13 7
BbeI G_GCGC'C
Cuts at: 2116 2364 2116
Size: 248 4434
BciVI GTATCCnnnnn_n'
Cuts at: 2580 3341 4682 2580
Size: 761 1341 2580
Fragments arranged by size:
2580 1341 761
BclI T'GATC_A
Cuts at: 2202 2202
Size: 4682
BglII A'GATC_T
Cuts at: 2197 4234 2197
Size: 2037 2645
BlpI GC'TnA_GC
Cuts at: 1180 1794 1180
Size: 614 4068
Bme1580I G_kGCm'C
Cuts at: 141 237 575 755 1254 1543 1564 1668 1676
Size: 96 338 180 499 289 21 104 8
Cuts at: 1676 2307 2400 3456 141
Size: 631 93 1056 1367
Fragments arranged by size:
1367 1056 631 499 338 289 180 104
96 93 21 8
BmgBI CAC'GTC
Cuts at: 1459 1459
Size: 4682
BmrI ACTGGGnnnn_n'
Cuts at: 1026 1257 1894 1975 2310 4454 1026
Size: 231 637 81 335 2144 1254
Fragments arranged by size:
2144 1254 637 335 231 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3049 3049
Size: 4682
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 932 1492 1499 1615 316
Size: 616 560 7 116 3383
Fragments arranged by size:
3383 616 560 116 7
Bpu10I CC'TnA_GC
Cuts at: 1493 1571 1493
Size: 78 4604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 1858 2737 3229 3527 853
Size: 1005 879 492 298 2008
Fragments arranged by size:
2008 1005 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 283 4386 283
Size: 4103 579
BsaAI yAC'GTr
Cuts at: 2035 2035
Size: 4682
BsaBI GATnn'nnATC
Cuts at: 6 2196 4233 6
Size: 2190 2037 455
Fragments arranged by size:
2190 2037 455
BsaHI Gr'CG_yC
Cuts at: 629 2113 2361 3063 4559 629
Size: 1484 248 702 1496 752
Fragments arranged by size:
1496 1484 752 702 248
BsaWI w'CCGG_w
Cuts at: 1871 2392 3070 3344 3491 3902 1871
Size: 521 678 274 147 411 2651
Fragments arranged by size:
2651 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1466 1466
Size: 4682
BseYI C'CCAG_C
Cuts at: 1221 1393 1661 2107 2865 3442 3938 1221
Size: 172 268 446 758 577 496 1965
Fragments arranged by size:
1965 758 577 496 446 268 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1412 1677 703
Size: 709 265 3708
Fragments arranged by size:
3708 709 265
BsiEI CG_ry'CG
Cuts at: 104 2224 2270 3478 104
Size: 2120 46 1208 1308
Fragments arranged by size:
2120 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 237 797 1186 1315 1494 1696 2474 2664 3456
Size: 560 389 129 179 202 778 190 792
Cuts at: 3456 237
Size: 1463
Fragments arranged by size:
1463 792 778 560 389 202 190 179
129
BsmI GAATG_Cn'
Cuts at: 1257 1257
Size: 4682
Bsp1286I G_dGCh'C
Cuts at: 141 237 575 734 755 797 1157 1186 1254
Size: 96 338 159 21 42 360 29 68
Cuts at: 1254 1315 1494 1543 1564 1577 1624 1631 1668
Size: 61 179 49 21 13 47 7 37
Cuts at: 1668 1676 1696 2307 2400 2474 2664 2726 3456
Size: 8 20 611 93 74 190 62 730
Cuts at: 3456 141
Size: 1367
Fragments arranged by size:
1367 730 611 360 338 190 179 159
96 93 74 68 62 61 49 47
42 37 29 21 21 20 13 8
7
BspEI T'CCGG_A
Cuts at: 3902 3902
Size: 4682
BspHI T'CATG_A
Cuts at: 3858 3858
Size: 4682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 327 602 725 1732 1946 2248 2629 327
Size: 275 123 1007 214 302 381 2380
Fragments arranged by size:
2380 1007 381 302 275 214 123
BsrBI CCG'CTC
Cuts at: 50 2974 3028 4512 4677 50
Size: 2924 54 1484 165 55
Fragments arranged by size:
2924 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2594 2594
Size: 4682
BsrFI r'CCGG_y
Cuts at: 2680 2861 3070 2680
Size: 181 209 4292
Fragments arranged by size:
4292 209 181
BssSI C'ACGA_G
Cuts at: 2953 3311 2953
Size: 358 4324
BstAPI GCAn_nnn'nTGC
Cuts at: 1547 2059 1547
Size: 512 4170
BstBI TT'CG_AA
Cuts at: 461 3043 461
Size: 2582 2100
BstEII G'GTnAC_C
Cuts at: 924 1056 924
Size: 132 4550
BstXI CCAn_nnnn'nTGG
Cuts at: 399 399
Size: 4682
BstYI r'GATC_y
Cuts at: 1 1707 2174 2197 2532 2778 3790 3876 3888
Size: 1706 467 23 335 246 1012 86 12
Cuts at: 3888 4234 1
Size: 346 449
Fragments arranged by size:
1706 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1583 1583
Size: 4682
BtgI C'CryG_G
Cuts at: 107 960 1067 107
Size: 853 107 3722
Fragments arranged by size:
3722 853 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1017 2632 2813 2976 1017
Size: 1615 181 163 2723
Fragments arranged by size:
2723 1615 181 163
BtsI GCAGTG_nn'
Cuts at: 1290 1346 1925 1290
Size: 56 579 4047
Fragments arranged by size:
4047 579 56
DraI TTT'AAA
Cuts at: 1681 1681
Size: 4682
DraIII CAC_nnn'GTG
Cuts at: 843 843
Size: 4682
DrdI GACnn_nn'nnGTC
Cuts at: 2388 3246 2388
Size: 858 3824
EaeI y'GGCC_r
Cuts at: 765 785 2267 2441 2832 2859 4204 765
Size: 20 1482 174 391 27 1345 1243
Fragments arranged by size:
1482 1345 1243 391 174 27 20
EagI C'GGCC_G
Cuts at: 2267 2267
Size: 4682
EarI CTCTTCn'nnn_
Cuts at: 179 802 1320 2705 2915 179
Size: 623 518 1385 210 1946
Fragments arranged by size:
1946 1385 623 518 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2890 3200 3346 4492 2890
Size: 310 146 1146 3080
Fragments arranged by size:
3080 1146 310 146
EcoICRI GAG'CTC
Cuts at: 795 1492 1694 795
Size: 697 202 3783
Fragments arranged by size:
3783 697 202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 932 1492 1499 1615 2507 2939 3686 3954
Size: 616 560 7 116 892 432 747 268
Cuts at: 3954 316
Size: 1044
Fragments arranged by size:
1044 892 747 616 560 432 268 116
7
EcoNI CCTnn'n_nnAGG
Cuts at: 334 1582 334
Size: 1248 3434
EcoO109I rG'GnC_Cy
Cuts at: 591 824 1526 1665 1821 591
Size: 233 702 139 156 3452
Fragments arranged by size:
3452 702 233 156 139
EcoRI G'AATT_C
Cuts at: 1686 1686
Size: 4682
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 4682
FspI TGC'GCA
Cuts at: 975 1084 2463 975
Size: 109 1379 3194
Fragments arranged by size:
3194 1379 109
FspAI rTGC'GCAy
Cuts at: 1084 1084
Size: 4682
HaeII r_GCGC'y
Cuts at: 774 1222 2116 2364 3386 4365 774
Size: 448 894 248 1022 979 1091
Fragments arranged by size:
1091 1022 979 894 448 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 156 188 2524 2554 2556 2586 156
Size: 32 2336 30 2 30 2252
Fragments arranged by size:
2336 2252 32 30 30 2
HincII GTy'rAC
Cuts at: 1721 1721
Size: 4682
KasI G'GCGC_C
Cuts at: 2112 2360 2112
Size: 248 4434
KpnI G_GTAC'C
Cuts at: 1165 1702 1165
Size: 537 4145
MluI A'CGCG_T
Cuts at: 4115 4115
Size: 4682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 199 3353 3537 199
Size: 3154 184 1344
Fragments arranged by size:
3154 1344 184
MscI TGG'CCA
Cuts at: 767 787 2443 767
Size: 20 1656 3006
Fragments arranged by size:
3006 1656 20
MslI CAynn'nnrTG
Cuts at: 397 1306 2671 2798 397
Size: 909 1365 127 2281
Fragments arranged by size:
2281 1365 909 127
MspA1I CmG'CkG
Cuts at: 1661 1793 2107 2467 3480 3725 1661
Size: 132 314 360 1013 245 2618
Fragments arranged by size:
2618 1013 360 314 245 132
NaeI GCC'GGC
Cuts at: 2863 2863
Size: 4682
NarI GG'CG_CC
Cuts at: 2113 2361 2113
Size: 248 4434
NcoI C'CATG_G
Cuts at: 107 960 1067 107
Size: 853 107 3722
Fragments arranged by size:
3722 853 107
NgoMIV G'CCGG_C
Cuts at: 2861 2861
Size: 4682
NspI r_CATG'y
Cuts at: 660 1550 1735 2235 3142 660
Size: 890 185 500 907 2200
Fragments arranged by size:
2200 907 890 500 185
PasI CC'CwG_GG
Cuts at: 1157 1624 1157
Size: 467 4215
PciI A'CATG_T
Cuts at: 3138 3138
Size: 4682
PflMI CCAn_nnn'nTGG
Cuts at: 275 339 740 763 774 275
Size: 64 401 23 11 4183
Fragments arranged by size:
4183 401 64 23 11
PmeI GTTT'AAAC
Cuts at: 1681 1681
Size: 4682
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 3821
Size: 32 4650
PpuMI rG'GwC_Cy
Cuts at: 591 591
Size: 4682
PspOMI G'GGCC_C
Cuts at: 1250 1560 1664 1250
Size: 310 104 4268
Fragments arranged by size:
4268 310 104
PstI C_TGCA'G
Cuts at: 1338 1729 2414 1338
Size: 391 685 3606
Fragments arranged by size:
3606 685 391
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4682
PvuII CAG'CTG
Cuts at: 1661 2107 2467 1661
Size: 446 360 3876
Fragments arranged by size:
3876 446 360
RsrII CG'GwC_CG
Cuts at: 2877 2877
Size: 4682
SacI G_AGCT'C
Cuts at: 797 1494 1696 797
Size: 697 202 3783
Fragments arranged by size:
3783 697 202
SalI G'TCGA_C
Cuts at: 1719 1719
Size: 4682
SapI GCTCTTCn'nnn_
Cuts at: 802 1320 2705 2915 802
Size: 518 1385 210 2569
Fragments arranged by size:
2569 1385 518 210
SbfI CC_TGCA'GG
Cuts at: 1729 1729
Size: 4682
ScaI AGT'ACT
Cuts at: 4199 4199
Size: 4682
SfcI C'TryA_G
Cuts at: 33 860 1334 1725 2410 3403 3594 33
Size: 827 474 391 685 993 191 1121
Fragments arranged by size:
1121 993 827 685 474 391 191
SfoI GGC'GCC
Cuts at: 2114 2362 2114
Size: 248 4434
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4682
SmaI CCC'GGG
Cuts at: 814 1363 1704 814
Size: 549 341 3792
Fragments arranged by size:
3792 549 341
SmlI C'TyrA_G
Cuts at: 868 1837 2752 3244 3506 868
Size: 969 915 492 262 2044
Fragments arranged by size:
2044 969 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4682
SphI G_CATG'C
Cuts at: 1735 2235 1735
Size: 500 4182
StyI C'CwwG_G
Cuts at: 107 228 723 960 1067 1264 1816 2170 107
Size: 121 495 237 107 197 552 354 2619
Fragments arranged by size:
2619 552 495 354 237 197 121 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 282 1201 1643 2265 2398 2857 3066 282
Size: 919 442 622 133 459 209 1898
Fragments arranged by size:
1898 919 622 459 442 209 133
TatI w'GTAC_w
Cuts at: 647 4197 647
Size: 3550 1132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1404 1421 1436 1453 4199 4231 1404
Size: 17 15 17 2746 32 1855
Fragments arranged by size:
2746 1855 32 17 17 15
Tth111I GACn'n_nGTC
Cuts at: 1460 2479 1460
Size: 1019 3663
XbaI T'CTAG_A
Cuts at: 1713 1713
Size: 4682
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 272 1039 1447 2177 272
Size: 767 408 730 2777
Fragments arranged by size:
2777 767 730 408
XmaI C'CCGG_G
Cuts at: 812 1361 1702 812
Size: 549 341 3792
Fragments arranged by size:
3792 549 341
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI