Restriction map for
pF1KB9611
(Circular) (Six-base) MAPSORT of: pF1KB9611.seq Check: 1216 from: 1 to: 4880
pF1KB9611 4880 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1874 1874
Size: 4880
AatII G_ACGT'C
Cuts at: 251 251
Size: 4880
AccI GT'mk_AC
Cuts at: 1918 1918
Size: 4880
Acc65I G'GTAC_C
Cuts at: 1896 1896
Size: 4880
AclI AA'CG_TT
Cuts at: 4720 4720
Size: 4880
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1459 1775 2705 3137 3884 4152 1459
Size: 316 930 432 747 268 2187
Fragments arranged by size:
2187 930 747 432 316 268
AflIII A'CryG_T
Cuts at: 2232 3336 4313 2232
Size: 1104 977 2799
Fragments arranged by size:
2799 1104 977
AgeI A'CCGG_T
Cuts at: 3268 3268
Size: 4880
AhdI GACnn_n'nnGTC
Cuts at: 1482 1482
Size: 4880
AleI CACnn'nnGTG
Cuts at: 1816 1816
Size: 4880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 583 583
Size: 4880
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1153 1185 1153
Size: 32 4848
AlwNI CAG_nnn'CTG
Cuts at: 965 1567 1856 2185 3752 965
Size: 602 289 329 1567 2093
Fragments arranged by size:
2093 1567 602 329 289
ApaLI G'TGCA_C
Cuts at: 628 3650 628
Size: 3022 1858
ApoI r'AATT_y
Cuts at: 1612 1884 4233 4489 1612
Size: 272 2349 256 2003
Fragments arranged by size:
2349 2003 272 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4880
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
AvaI C'yCGr_G
Cuts at: 699 796 1900 699
Size: 97 1104 3679
Fragments arranged by size:
3679 1104 97
BamHI G'GATC_C
Cuts at: 932 1905 932
Size: 973 3907
BanI G'GyrC_C
Cuts at: 1221 1896 2310 2558 2593 1221
Size: 675 414 248 35 3508
Fragments arranged by size:
3508 675 414 248 35
BanII G_rGCy'C
Cuts at: 803 1156 1894 2924 803
Size: 353 738 1030 2759
Fragments arranged by size:
2759 1030 738 353
BbeI G_GCGC'C
Cuts at: 1225 2314 2562 1225
Size: 1089 248 3543
Fragments arranged by size:
3543 1089 248
BbsI GAAGACnn'nnnn_
Cuts at: 729 1305 729
Size: 576 4304
BbvCI CC'TCA_GC
Cuts at: 1113 1113
Size: 4880
BciVI GTATCCnnnnn_n'
Cuts at: 2778 3539 4880 2778
Size: 761 1341 2778
Fragments arranged by size:
2778 1341 761
BclI T'GATC_A
Cuts at: 2400 2400
Size: 4880
BglI GCCn_nnn'nGGC
Cuts at: 298 1627 298
Size: 1329 3551
BglII A'GATC_T
Cuts at: 1034 1586 2395 4432 1034
Size: 552 809 2037 1482
Fragments arranged by size:
2037 1482 809 552
BlpI GC'TnA_GC
Cuts at: 1254 1259 1992 1254
Size: 5 733 4142
Fragments arranged by size:
4142 733 5
Bme1580I G_kGCm'C
Cuts at: 632 972 1543 1823 2505 2598 3654 632
Size: 340 571 280 682 93 1056 1858
Fragments arranged by size:
1858 1056 682 571 340 280 93
BmrI ACTGGGnnnn_n'
Cuts at: 2092 2173 2508 4652 2092
Size: 81 335 2144 2320
Fragments arranged by size:
2320 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 873 3247 873
Size: 2374 2506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 547 733 736 793 843 1285 421
Size: 126 186 3 57 50 442 4016
Fragments arranged by size:
4016 442 186 126 57 50 3
Bpu10I CC'TnA_GC
Cuts at: 1113 1113
Size: 4880
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2056 2935 3427 3725 2056
Size: 879 492 298 3211
Fragments arranged by size:
3211 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 121 709 1173 1642 4584 121
Size: 588 464 469 2942 417
Fragments arranged by size:
2942 588 469 464 417
BsaAI yAC'GTr
Cuts at: 2233 2233
Size: 4880
BsaBI GATnn'nnATC
Cuts at: 6 1591 2394 4431 6
Size: 1585 803 2037 455
Fragments arranged by size:
2037 1585 803 455
BsaHI Gr'CG_yC
Cuts at: 248 578 1222 1406 2311 2559 3261 4757 248
Size: 330 644 184 905 248 702 1496 371
Fragments arranged by size:
1496 905 702 644 371 330 248 184
BsaWI w'CCGG_w
Cuts at: 2069 2590 3268 3542 3689 4100 2069
Size: 521 678 274 147 411 2849
Fragments arranged by size:
2849 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 169 199 1153 1183 1504 1534 169
Size: 30 954 30 321 30 3515
Fragments arranged by size:
3515 954 321 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 417 463 793 862 417
Size: 46 330 69 4435
Fragments arranged by size:
4435 330 69 46
BseYI C'CCAG_C
Cuts at: 1087 1544 2305 3063 3640 4136 1087
Size: 457 761 758 577 496 1831
Fragments arranged by size:
1831 761 758 577 496 457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 370
Size: 4880
BsiEI CG_ry'CG
Cuts at: 104 270 1121 2422 2468 3676 104
Size: 166 851 1301 46 1208 1308
Fragments arranged by size:
1308 1301 1208 851 166 46
BsiHKAI G_wGCw'C
Cuts at: 632 803 1894 2672 2862 3654 632
Size: 171 1091 778 190 792 1858
Fragments arranged by size:
1858 1091 792 778 190 171
BsmBI CGTCTCn'nnnn_
Cuts at: 631 861 631
Size: 230 4650
Bsp1286I G_dGCh'C
Cuts at: 632 803 972 1156 1543 1823 1894 2505 2598
Size: 171 169 184 387 280 71 611 93
Cuts at: 2598 2672 2862 2924 3654 632
Size: 74 190 62 730 1858
Fragments arranged by size:
1858 730 611 387 280 190 184 171
169 93 74 71 62
BspEI T'CCGG_A
Cuts at: 4100 4100
Size: 4880
BspHI T'CATG_A
Cuts at: 4056 4056
Size: 4880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 392 1091 1874 1930 2144 2446 2827 392
Size: 699 783 56 214 302 381 2445
Fragments arranged by size:
2445 783 699 381 302 214 56
BsrBI CCG'CTC
Cuts at: 50 729 3172 3226 4710 4875 50
Size: 679 2443 54 1484 165 55
Fragments arranged by size:
2443 1484 679 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1419 2792 1419
Size: 1373 3507
BsrFI r'CCGG_y
Cuts at: 593 2878 3059 3268 593
Size: 2285 181 209 2205
Fragments arranged by size:
2285 2205 209 181
BssSI C'ACGA_G
Cuts at: 3151 3509 3151
Size: 358 4522
BstAPI GCAn_nnn'nTGC
Cuts at: 233 1697 2257 233
Size: 1464 560 2856
Fragments arranged by size:
2856 1464 560
BstBI TT'CG_AA
Cuts at: 3241 3241
Size: 4880
BstEII G'GTnAC_C
Cuts at: 954 954
Size: 4880
BstXI CCAn_nnnn'nTGG
Cuts at: 555 555
Size: 4880
BstYI r'GATC_y
Cuts at: 1 932 1034 1586 1905 2372 2395 2730 2976
Size: 931 102 552 319 467 23 335 246
Cuts at: 2976 3988 4074 4086 4432 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 931 552 467 449 346 335 319
246 102 86 23 12
BtgI C'CryG_G
Cuts at: 336 778 1248 1838 1871 336
Size: 442 470 590 33 3345
Fragments arranged by size:
3345 590 470 442 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 420 2830 3011 3174 420
Size: 2410 181 163 2126
Fragments arranged by size:
2410 2126 181 163
BtsI GCAGTG_nn'
Cuts at: 231 682 1427 2123 231
Size: 451 745 696 2988
Fragments arranged by size:
2988 745 696 451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1827 1862 1827
Size: 35 4845
DraI TTT'AAA
Cuts at: 1879 1879
Size: 4880
DraIII CAC_nnn'GTG
Cuts at: 501 501
Size: 4880
DrdI GACnn_nn'nnGTC
Cuts at: 135 2586 3444 135
Size: 2451 858 1571
Fragments arranged by size:
2451 1571 858
EaeI y'GGCC_r
Cuts at: 299 973 1245 2465 2639 3030 3057 4402 299
Size: 674 272 1220 174 391 27 1345 777
Fragments arranged by size:
1345 1220 777 674 391 272 174 27
EagI C'GGCC_G
Cuts at: 2465 2465
Size: 4880
EarI CTCTTCn'nnn_
Cuts at: 178 2903 3113 178
Size: 2725 210 1945
Fragments arranged by size:
2725 1945 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3088 3398 3544 4690 3088
Size: 310 146 1146 3278
Fragments arranged by size:
3278 1146 310 146
EcoICRI GAG'CTC
Cuts at: 801 1892 801
Size: 1091 3789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 547 733 736 793 843 1285 1459 1775
Size: 126 186 3 57 50 442 174 316
Cuts at: 1775 2705 3137 3884 4152 421
Size: 930 432 747 268 1149
Fragments arranged by size:
1149 930 747 442 432 316 268 186
174 126 57 50 3
EcoNI CCTnn'n_nnAGG
Cuts at: 673 847 673
Size: 174 4706
EcoO109I rG'GnC_Cy
Cuts at: 867 912 1751 2019 867
Size: 45 839 268 3728
Fragments arranged by size:
3728 839 268 45
EcoRI G'AATT_C
Cuts at: 1612 1884 1612
Size: 272 4608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 807 2899 807
Size: 2092 2788
FspI TGC'GCA
Cuts at: 2661 2661
Size: 4880
HaeII r_GCGC'y
Cuts at: 1112 1225 2314 2562 3584 4563 1112
Size: 113 1089 248 1022 979 1429
Fragments arranged by size:
1429 1089 1022 979 248 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 682 714 1195 1227 1468 1500 2722 2752 2754
Size: 32 481 32 241 32 1222 30 2
Cuts at: 2754 2784 682
Size: 30 2778
Fragments arranged by size:
2778 1222 481 241 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 369 1919 369
Size: 1550 3330
HindIII A'AGCT_T
Cuts at: 895 895
Size: 4880
KasI G'GCGC_C
Cuts at: 1221 2310 2558 1221
Size: 1089 248 3543
Fragments arranged by size:
3543 1089 248
KpnI G_GTAC'C
Cuts at: 1900 1900
Size: 4880
MluI A'CGCG_T
Cuts at: 4313 4313
Size: 4880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 449 572 860 3551 3735 449
Size: 123 288 2691 184 1594
Fragments arranged by size:
2691 1594 288 184 123
MscI TGG'CCA
Cuts at: 1247 2641 1247
Size: 1394 3486
MslI CAynn'nnrTG
Cuts at: 1816 2869 2996 1816
Size: 1053 127 3700
Fragments arranged by size:
3700 1053 127
MspA1I CmG'CkG
Cuts at: 1258 1567 1873 1991 2305 2665 3678 3923 1258
Size: 309 306 118 314 360 1013 245 2215
Fragments arranged by size:
2215 1013 360 314 309 306 245 118
NaeI GCC'GGC
Cuts at: 595 3061 595
Size: 2466 2414
NarI GG'CG_CC
Cuts at: 1222 2311 2559 1222
Size: 1089 248 3543
Fragments arranged by size:
3543 1089 248
NcoI C'CATG_G
Cuts at: 336 1248 336
Size: 912 3968
NgoMIV G'CCGG_C
Cuts at: 593 3059 593
Size: 2466 2414
NspI r_CATG'y
Cuts at: 240 486 621 1695 1933 2433 3340 240
Size: 246 135 1074 238 500 907 1780
Fragments arranged by size:
1780 1074 907 500 246 238 135
PasI CC'CwG_GG
Cuts at: 1363 1363
Size: 4880
PciI A'CATG_T
Cuts at: 3336 3336
Size: 4880
PflMI CCAn_nnn'nTGG
Cuts at: 1376 1658 1707 1376
Size: 282 49 4549
Fragments arranged by size:
4549 282 49
PfoI T'CCnGG_A
Cuts at: 374 524 374
Size: 150 4730
PmeI GTTT'AAAC
Cuts at: 1879 1879
Size: 4880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 313 345 1153 1185 4019 4051 313
Size: 32 808 32 2834 32 1142
Fragments arranged by size:
2834 1142 808 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1751 1751
Size: 4880
PstI C_TGCA'G
Cuts at: 491 848 941 1927 2612 491
Size: 357 93 986 685 2759
Fragments arranged by size:
2759 986 685 357 93
PvuI CG_AT'CG
Cuts at: 104 1121 104
Size: 1017 3863
PvuII CAG'CTG
Cuts at: 1258 2305 2665 1258
Size: 1047 360 3473
Fragments arranged by size:
3473 1047 360
RsrII CG'GwC_CG
Cuts at: 3075 3075
Size: 4880
SacI G_AGCT'C
Cuts at: 803 1894 803
Size: 1091 3789
SacII CC_GC'GG
Cuts at: 1874 1874
Size: 4880
SalI G'TCGA_C
Cuts at: 1917 1917
Size: 4880
SanDI GG'GwC_CC
Cuts at: 1751 1751
Size: 4880
SapI GCTCTTCn'nnn_
Cuts at: 2903 3113 2903
Size: 210 4670
SbfI CC_TGCA'GG
Cuts at: 848 1927 848
Size: 1079 3801
ScaI AGT'ACT
Cuts at: 4397 4397
Size: 4880
SexAI A'CCwGG_T
Cuts at: 1040 1040
Size: 4880
SfcI C'TryA_G
Cuts at: 33 487 844 937 1923 2608 3601 3792 33
Size: 454 357 93 986 685 993 191 1121
Fragments arranged by size:
1121 993 986 685 454 357 191 93
SfoI GGC'GCC
Cuts at: 1223 2312 2560 1223
Size: 1089 248 3543
Fragments arranged by size:
3543 1089 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
SmaI CCC'GGG
Cuts at: 1902 1902
Size: 4880
SmlI C'TyrA_G
Cuts at: 699 2035 2950 3442 3704 699
Size: 1336 915 492 262 1875
Fragments arranged by size:
1875 1336 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4880
SphI G_CATG'C
Cuts at: 1933 2433 1933
Size: 500 4380
StuI AGG'CCT
Cuts at: 184 184
Size: 4880
StyI C'CwwG_G
Cuts at: 336 674 1058 1073 1172 1214 1248 1487 1549
Size: 338 384 15 99 42 34 239 62
Cuts at: 1549 2014 2368 336
Size: 465 354 2848
Fragments arranged by size:
2848 465 384 354 338 239 99 62
42 34 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 102 733 747 1173 2463 2596 3055 3264 102
Size: 631 14 426 1290 133 459 209 1718
Fragments arranged by size:
1718 1290 631 459 426 209 133 14
TatI w'GTAC_w
Cuts at: 4395 4395
Size: 4880
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 169 201 770 802 1504 1536 4397 4429 169
Size: 32 569 32 702 32 2861 32 620
Fragments arranged by size:
2861 702 620 569 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 364 2677 364
Size: 2313 2567
XbaI T'CTAG_A
Cuts at: 1911 1911
Size: 4880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 900 2375 900
Size: 1475 3405
XhoI C'TCGA_G
Cuts at: 699 699
Size: 4880
XmaI C'CCGG_G
Cuts at: 1900 1900
Size: 4880
XmnI GAAnn'nnTTC
Cuts at: 538 1102 538
Size: 564 4316
ZraI GAC'GTC
Cuts at: 249 249
Size: 4880
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII ApaI AscI AvrII BaeI BcgI BfrBI
BmgBI BmtI BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I
ClaI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspOMI
PspXI PsrI SfiI SgrAI SnaBI SrfI SspI SwaI