Restriction map for
pF1KB9619
(Circular) (Six-base) MAPSORT of: pF1KB9619.seq Check: 9059 from: 1 to: 4154
pF1KB9619 4154 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1056 1056
Size: 4154
AccI GT'mk_AC
Cuts at: 623 1192 623
Size: 569 3585
Acc65I G'GTAC_C
Cuts at: 1170 1170
Size: 4154
AclI AA'CG_TT
Cuts at: 3994 3994
Size: 4154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1979 2411 3158 3426 1979
Size: 432 747 268 2707
Fragments arranged by size:
2707 747 432 268
AflIII A'CryG_T
Cuts at: 575 1506 2610 3587 575
Size: 931 1104 977 1142
Fragments arranged by size:
1142 1104 977 931
AgeI A'CCGG_T
Cuts at: 2542 2542
Size: 4154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 949 949
Size: 4154
AlwNI CAG_nnn'CTG
Cuts at: 682 1122 1459 3026 682
Size: 440 337 1567 1810
Fragments arranged by size:
1810 1567 440 337
ApaI G_GGCC'C
Cuts at: 177 602 614 177
Size: 425 12 3717
Fragments arranged by size:
3717 425 12
ApaLI G'TGCA_C
Cuts at: 111 2924 111
Size: 2813 1341
ApoI r'AATT_y
Cuts at: 1158 3507 3763 1158
Size: 2349 256 1549
Fragments arranged by size:
2349 1549 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4154
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
AvaI C'yCGr_G
Cuts at: 423 613 690 1174 423
Size: 190 77 484 3403
Fragments arranged by size:
3403 484 190 77
BamHI G'GATC_C
Cuts at: 1179 1179
Size: 4154
BanI G'GyrC_C
Cuts at: 697 811 1170 1584 1832 1867 697
Size: 114 359 414 248 35 2984
Fragments arranged by size:
2984 414 359 248 114 35
BanII G_rGCy'C
Cuts at: 177 273 328 602 614 727 1168 2198 177
Size: 96 55 274 12 113 441 1030 2133
Fragments arranged by size:
2133 1030 441 274 113 96 55 12
BbeI G_GCGC'C
Cuts at: 701 815 1588 1836 701
Size: 114 773 248 3019
Fragments arranged by size:
3019 773 248 114
BbsI GAAGACnn'nnnn_
Cuts at: 905 1015 905
Size: 110 4044
BbvCI CC'TCA_GC
Cuts at: 661 661
Size: 4154
BciVI GTATCCnnnnn_n'
Cuts at: 157 2052 2813 4154 157
Size: 1895 761 1341 157
Fragments arranged by size:
1895 1341 761 157
BclI T'GATC_A
Cuts at: 401 1674 401
Size: 1273 2881
BglI GCCn_nnn'nGGC
Cuts at: 290 290
Size: 4154
BglII A'GATC_T
Cuts at: 1669 3706 1669
Size: 2037 2117
BlpI GC'TnA_GC
Cuts at: 1266 1266
Size: 4154
Bme1580I G_kGCm'C
Cuts at: 115 177 602 614 1779 1872 2928 115
Size: 62 425 12 1165 93 1056 1341
Fragments arranged by size:
1341 1165 1056 425 93 62 12
BmgBI CAC'GTC
Cuts at: 1101 1101
Size: 4154
BmrI ACTGGGnnnn_n'
Cuts at: 1366 1447 1782 3926 1366
Size: 81 335 2144 1594
Fragments arranged by size:
2144 1594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2521 2521
Size: 4154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 1015 997
Size: 18 4136
Bpu10I CC'TnA_GC
Cuts at: 661 661
Size: 4154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 1000 1330 2209 2701 2999 331
Size: 669 330 879 492 298 1486
Fragments arranged by size:
1486 879 669 492 330 298
BsaI GGTCTCn'nnnn_
Cuts at: 3858 3858
Size: 4154
BsaAI yAC'GTr
Cuts at: 561 1507 561
Size: 946 3208
BsaBI GATnn'nnATC
Cuts at: 6 1668 3705 6
Size: 1662 2037 455
Fragments arranged by size:
2037 1662 455
BsaHI Gr'CG_yC
Cuts at: 412 488 620 698 812 866 1585 1833 2535
Size: 76 132 78 114 54 719 248 702
Cuts at: 2535 4031 412
Size: 1496 535
Fragments arranged by size:
1496 719 702 535 248 132 114 78
76 54
BsaWI w'CCGG_w
Cuts at: 248 1343 1864 2542 2816 2963 3374 248
Size: 1095 521 678 274 147 411 1028
Fragments arranged by size:
1095 1028 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 511 1045 511
Size: 534 3620
BseYI C'CCAG_C
Cuts at: 607 675 795 1073 1579 2337 2914 3410 607
Size: 68 120 278 506 758 577 496 1351
Fragments arranged by size:
1351 758 577 506 496 278 120 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 529 488
Size: 41 4113
BsiEI CG_ry'CG
Cuts at: 104 171 1696 1742 2950 104
Size: 67 1525 46 1208 1308
Fragments arranged by size:
1525 1308 1208 67 46
BsiHKAI G_wGCw'C
Cuts at: 115 727 1168 1946 2136 2928 115
Size: 612 441 778 190 792 1341
Fragments arranged by size:
1341 792 778 612 441 190
BsmBI CGTCTCn'nnnn_
Cuts at: 347 347
Size: 4154
Bsp1286I G_dGCh'C
Cuts at: 115 177 273 328 602 614 727 1168 1779
Size: 62 96 55 274 12 113 441 611
Cuts at: 1779 1872 1946 2136 2198 2928 115
Size: 93 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 441 274 190 113 96
93 74 62 62 55 12
BspEI T'CCGG_A
Cuts at: 3374 3374
Size: 4154
BspHI T'CATG_A
Cuts at: 1091 3330 1091
Size: 2239 1915
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1056 1204 1418 1720 2101 1056
Size: 148 214 302 381 3109
Fragments arranged by size:
3109 381 302 214 148
BsrBI CCG'CTC
Cuts at: 50 2446 2500 3984 4149 50
Size: 2396 54 1484 165 55
Fragments arranged by size:
2396 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2066 2066
Size: 4154
BsrFI r'CCGG_y
Cuts at: 334 628 775 876 941 1048 2152 2333 2542
Size: 294 147 101 65 107 1104 181 209
Cuts at: 2542 334
Size: 1946
Fragments arranged by size:
1946 1104 294 209 181 147 107 101
65
BssHII G'CGCG_C
Cuts at: 853 988 853
Size: 135 4019
BssSI C'ACGA_G
Cuts at: 2425 2783 2425
Size: 358 3796
BstAPI GCAn_nnn'nTGC
Cuts at: 843 1531 843
Size: 688 3466
BstBI TT'CG_AA
Cuts at: 2515 2515
Size: 4154
BstXI CCAn_nnnn'nTGG
Cuts at: 894 894
Size: 4154
BstYI r'GATC_y
Cuts at: 1 1179 1646 1669 2004 2250 3262 3348 3360
Size: 1178 467 23 335 246 1012 86 12
Cuts at: 3360 3706 1
Size: 346 449
Fragments arranged by size:
1178 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 444 807 444
Size: 363 3791
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 969 2104 2285 2448 969
Size: 1135 181 163 2675
Fragments arranged by size:
2675 1135 181 163
BtsI GCAGTG_nn'
Cuts at: 1397 1397
Size: 4154
DraI TTT'AAA
Cuts at: 1153 1153
Size: 4154
DraIII CAC_nnn'GTG
Cuts at: 959 959
Size: 4154
DrdI GACnn_nn'nnGTC
Cuts at: 523 1860 2718 523
Size: 1337 858 1959
Fragments arranged by size:
1959 1337 858
EaeI y'GGCC_r
Cuts at: 168 236 780 950 1739 1913 2304 2331 3676
Size: 68 544 170 789 174 391 27 1345
Cuts at: 3676 168
Size: 646
Fragments arranged by size:
1345 789 646 544 391 174 170 68
27
EagI C'GGCC_G
Cuts at: 168 1739 168
Size: 1571 2583
EarI CTCTTCn'nnn_
Cuts at: 363 2177 2387 363
Size: 1814 210 2130
Fragments arranged by size:
2130 1814 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2362 2672 2818 3964 2362
Size: 310 146 1146 2552
Fragments arranged by size:
2552 1146 310 146
EcoICRI GAG'CTC
Cuts at: 725 1166 725
Size: 441 3713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 1015 1979 2411 3158 3426 997
Size: 18 964 432 747 268 1725
Fragments arranged by size:
1725 964 747 432 268 18
EcoO109I rG'GnC_Cy
Cuts at: 174 598 599 1293 174
Size: 424 1 694 3035
Fragments arranged by size:
3035 694 424 1
EcoRI G'AATT_C
Cuts at: 1158 1158
Size: 4154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 4154
FspI TGC'GCA
Cuts at: 1935 1935
Size: 4154
HaeII r_GCGC'y
Cuts at: 265 590 701 815 1588 1836 2858 3837 265
Size: 325 111 114 773 248 1022 979 582
Fragments arranged by size:
1022 979 773 582 325 248 114 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1996 2026 2028 2058 1996
Size: 30 2 30 4092
Fragments arranged by size:
4092 30 30 2
HincII GTy'rAC
Cuts at: 231 1193 231
Size: 962 3192
KasI G'GCGC_C
Cuts at: 697 811 1584 1832 697
Size: 114 773 248 3019
Fragments arranged by size:
3019 773 248 114
KpnI G_GTAC'C
Cuts at: 1174 1174
Size: 4154
MluI A'CGCG_T
Cuts at: 3587 3587
Size: 4154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2825 3009 2825
Size: 184 3970
MscI TGG'CCA
Cuts at: 782 1915 782
Size: 1133 3021
MslI CAynn'nnrTG
Cuts at: 118 892 916 2143 2270 118
Size: 774 24 1227 127 2002
Fragments arranged by size:
2002 1227 774 127 24
MspA1I CmG'CkG
Cuts at: 446 458 795 809 1119 1265 1579 1939 2952
Size: 12 337 14 310 146 314 360 1013
Cuts at: 2952 3197 446
Size: 245 1403
Fragments arranged by size:
1403 1013 360 337 314 310 245 146
14 12
NaeI GCC'GGC
Cuts at: 630 1050 2335 630
Size: 420 1285 2449
Fragments arranged by size:
2449 1285 420
NarI GG'CG_CC
Cuts at: 698 812 1585 1833 698
Size: 114 773 248 3019
Fragments arranged by size:
3019 773 248 114
NgoMIV G'CCGG_C
Cuts at: 628 1048 2333 628
Size: 420 1285 2449
Fragments arranged by size:
2449 1285 420
NspI r_CATG'y
Cuts at: 1207 1707 2614 1207
Size: 500 907 2747
Fragments arranged by size:
2747 907 500
PasI CC'CwG_GG
Cuts at: 582 582
Size: 4154
PciI A'CATG_T
Cuts at: 2610 2610
Size: 4154
PfoI T'CCnGG_A
Cuts at: 1058 1058
Size: 4154
PmeI GTTT'AAAC
Cuts at: 1153 1153
Size: 4154
PmlI CAC'GTG
Cuts at: 561 561
Size: 4154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 4122
PshAI GACnn'nnGTC
Cuts at: 1099 1099
Size: 4154
PspOMI G'GGCC_C
Cuts at: 173 598 610 173
Size: 425 12 3717
Fragments arranged by size:
3717 425 12
PstI C_TGCA'G
Cuts at: 1201 1886 1201
Size: 685 3469
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4154
PvuII CAG'CTG
Cuts at: 795 1119 1579 1939 795
Size: 324 460 360 3010
Fragments arranged by size:
3010 460 360 324
RsrII CG'GwC_CG
Cuts at: 2349 2349
Size: 4154
SacI G_AGCT'C
Cuts at: 727 1168 727
Size: 441 3713
SacII CC_GC'GG
Cuts at: 447 810 447
Size: 363 3791
SalI G'TCGA_C
Cuts at: 1191 1191
Size: 4154
SapI GCTCTTCn'nnn_
Cuts at: 2177 2387 2177
Size: 210 3944
SbfI CC_TGCA'GG
Cuts at: 1201 1201
Size: 4154
ScaI AGT'ACT
Cuts at: 433 3671 433
Size: 3238 916
SfcI C'TryA_G
Cuts at: 33 1197 1882 2875 3066 33
Size: 1164 685 993 191 1121
Fragments arranged by size:
1164 1121 993 685 191
SfoI GGC'GCC
Cuts at: 699 813 1586 1834 699
Size: 114 773 248 3019
Fragments arranged by size:
3019 773 248 114
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
SgrAI Cr'CCGG_yG
Cuts at: 334 775 876 334
Size: 441 101 3612
Fragments arranged by size:
3612 441 101
SmaI CCC'GGG
Cuts at: 425 615 1176 425
Size: 190 561 3403
Fragments arranged by size:
3403 561 190
SmlI C'TyrA_G
Cuts at: 346 1015 1309 2224 2716 2978 346
Size: 669 294 915 492 262 1522
Fragments arranged by size:
1522 915 669 492 294 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4154
SphI G_CATG'C
Cuts at: 1207 1707 1207
Size: 500 3654
StyI C'CwwG_G
Cuts at: 1288 1642 1288
Size: 354 3800
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1737 1870 2329 2538 1737
Size: 133 459 209 3353
Fragments arranged by size:
3353 459 209 133
TatI w'GTAC_w
Cuts at: 431 3669 431
Size: 3238 916
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3671 3703 3671
Size: 32 4122
Tth111I GACn'n_nGTC
Cuts at: 1951 1951
Size: 4154
XbaI T'CTAG_A
Cuts at: 1185 1185
Size: 4154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 579 1649 579
Size: 1070 3084
XmaI C'CCGG_G
Cuts at: 423 613 1174 423
Size: 190 561 3403
Fragments arranged by size:
3403 561 190
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PfoI PmeI PmlI PpiI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsaXI BsiWI BsmI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PpuMI PsiI PspXI PsrI
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI