Restriction map for
pF1KB9629
(Circular) (Six-base) MAPSORT of: pF1KB9629.seq Check: 7795 from: 1 to: 4124
pF1KB9629 4124 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1162 1162
Size: 4124
Acc65I G'GTAC_C
Cuts at: 1140 1140
Size: 4124
AclI AA'CG_TT
Cuts at: 3964 3964
Size: 4124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1949 2381 3128 3396 946
Size: 1003 432 747 268 1674
Fragments arranged by size:
1674 1003 747 432 268
AflII C'TTAA_G
Cuts at: 456 885 456
Size: 429 3695
AflIII A'CryG_T
Cuts at: 1476 2580 3557 1476
Size: 1104 977 2043
Fragments arranged by size:
2043 1104 977
AgeI A'CCGG_T
Cuts at: 2512 2512
Size: 4124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 998 998
Size: 4124
AlwNI CAG_nnn'CTG
Cuts at: 495 1429 2996 495
Size: 934 1567 1623
Fragments arranged by size:
1623 1567 934
ApaI G_GGCC'C
Cuts at: 261 261
Size: 4124
ApaLI G'TGCA_C
Cuts at: 2894 2894
Size: 4124
ApoI r'AATT_y
Cuts at: 551 1128 3477 3733 551
Size: 577 2349 256 942
Fragments arranged by size:
2349 942 577 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4124
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
AvaI C'yCGr_G
Cuts at: 765 1144 765
Size: 379 3745
BamHI G'GATC_C
Cuts at: 1149 1149
Size: 4124
BanI G'GyrC_C
Cuts at: 1015 1140 1554 1802 1837 1015
Size: 125 414 248 35 3302
Fragments arranged by size:
3302 414 248 125 35
BanII G_rGCy'C
Cuts at: 261 755 1138 2168 261
Size: 494 383 1030 2217
Fragments arranged by size:
2217 1030 494 383
BbeI G_GCGC'C
Cuts at: 1558 1806 1558
Size: 248 3876
BbsI GAAGACnn'nnnn_
Cuts at: 302 302
Size: 4124
BciVI GTATCCnnnnn_n'
Cuts at: 2022 2783 4124 2022
Size: 761 1341 2022
Fragments arranged by size:
2022 1341 761
BclI T'GATC_A
Cuts at: 1644 1644
Size: 4124
BfrBI ATG'CAT
Cuts at: 975 975
Size: 4124
BglII A'GATC_T
Cuts at: 576 1639 3676 576
Size: 1063 2037 1024
Fragments arranged by size:
2037 1063 1024
BlpI GC'TnA_GC
Cuts at: 1236 1236
Size: 4124
Bme1580I G_kGCm'C
Cuts at: 261 543 1749 1842 2898 261
Size: 282 1206 93 1056 1487
Fragments arranged by size:
1487 1206 1056 282 93
BmrI ACTGGGnnnn_n'
Cuts at: 1336 1417 1752 3896 1336
Size: 81 335 2144 1564
Fragments arranged by size:
2144 1564 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 4124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 284
Size: 4124
Bpu10I CC'TnA_GC
Cuts at: 749 749
Size: 4124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1300 2179 2671 2969 1300
Size: 879 492 298 2455
Fragments arranged by size:
2455 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 564 3828 564
Size: 3264 860
BsaAI yAC'GTr
Cuts at: 1477 1477
Size: 4124
BsaBI GATnn'nnATC
Cuts at: 6 1638 3675 6
Size: 1632 2037 455
Fragments arranged by size:
2037 1632 455
BsaHI Gr'CG_yC
Cuts at: 1555 1803 2505 4001 1555
Size: 248 702 1496 1678
Fragments arranged by size:
1678 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1313 1834 2512 2786 2933 3344 1313
Size: 521 678 274 147 411 2093
Fragments arranged by size:
2093 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 304 304
Size: 4124
BseYI C'CCAG_C
Cuts at: 617 1549 2307 2884 3380 617
Size: 932 758 577 496 1361
Fragments arranged by size:
1361 932 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 680 1004 180
Size: 500 324 3300
Fragments arranged by size:
3300 500 324
BsiEI CG_ry'CG
Cuts at: 104 1666 1712 2920 104
Size: 1562 46 1208 1308
Fragments arranged by size:
1562 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 755 1138 1916 2106 2898 755
Size: 383 778 190 792 1981
Fragments arranged by size:
1981 792 778 383 190
BsmI GAATG_Cn'
Cuts at: 184 184
Size: 4124
Bsp1286I G_dGCh'C
Cuts at: 261 543 755 1138 1749 1842 1916 2106 2168
Size: 282 212 383 611 93 74 190 62
Cuts at: 2168 2898 261
Size: 730 1487
Fragments arranged by size:
1487 730 611 383 282 212 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3344 3344
Size: 4124
BspHI T'CATG_A
Cuts at: 3300 3300
Size: 4124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1174 1388 1690 2071 1174
Size: 214 302 381 3227
Fragments arranged by size:
3227 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2416 2470 3954 4119 50
Size: 2366 54 1484 165 55
Fragments arranged by size:
2366 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2036 2036
Size: 4124
BsrFI r'CCGG_y
Cuts at: 2122 2303 2512 2122
Size: 181 209 3734
Fragments arranged by size:
3734 209 181
BssHII G'CGCG_C
Cuts at: 409 409
Size: 4124
BssSI C'ACGA_G
Cuts at: 2395 2753 2395
Size: 358 3766
BstAPI GCAn_nnn'nTGC
Cuts at: 1501 1501
Size: 4124
BstBI TT'CG_AA
Cuts at: 2485 2485
Size: 4124
BstEII G'GTnAC_C
Cuts at: 710 710
Size: 4124
BstYI r'GATC_y
Cuts at: 1 576 1149 1616 1639 1974 2220 3232 3318
Size: 575 573 467 23 335 246 1012 86
Cuts at: 3318 3330 3676 1
Size: 12 346 449
Fragments arranged by size:
1012 575 573 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2074 2255 2418 2074
Size: 181 163 3780
Fragments arranged by size:
3780 181 163
BtsI GCAGTG_nn'
Cuts at: 825 992 1367 825
Size: 167 375 3582
Fragments arranged by size:
3582 375 167
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 617 652 617
Size: 35 4089
DraI TTT'AAA
Cuts at: 1123 1123
Size: 4124
DrdI GACnn_nn'nnGTC
Cuts at: 1830 2688 1830
Size: 858 3266
EaeI y'GGCC_r
Cuts at: 521 1709 1883 2274 2301 3646 521
Size: 1188 174 391 27 1345 999
Fragments arranged by size:
1345 1188 999 391 174 27
EagI C'GGCC_G
Cuts at: 1709 1709
Size: 4124
EarI CTCTTCn'nnn_
Cuts at: 281 299 2147 2357 281
Size: 18 1848 210 2048
Fragments arranged by size:
2048 1848 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2332 2642 2788 3934 2332
Size: 310 146 1146 2522
Fragments arranged by size:
2522 1146 310 146
EcoICRI GAG'CTC
Cuts at: 753 1136 753
Size: 383 3741
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 946 1949 2381 3128 3396 284
Size: 662 1003 432 747 268 1012
Fragments arranged by size:
1012 1003 747 662 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1009 1009
Size: 4124
EcoO109I rG'GnC_Cy
Cuts at: 257 258 880 1000 1263 257
Size: 1 622 120 263 3118
Fragments arranged by size:
3118 622 263 120 1
EcoRI G'AATT_C
Cuts at: 1128 1128
Size: 4124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 4124
FspI TGC'GCA
Cuts at: 1069 1905 1069
Size: 836 3288
HaeII r_GCGC'y
Cuts at: 1558 1806 2828 3807 1558
Size: 248 1022 979 1875
Fragments arranged by size:
1875 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 561 593 1966 1996 1998 2028 561
Size: 32 1373 30 2 30 2657
Fragments arranged by size:
2657 1373 32 30 30 2
HincII GTy'rAC
Cuts at: 113 1163 113
Size: 1050 3074
HindIII A'AGCT_T
Cuts at: 213 213
Size: 4124
HpaI GTT'AAC
Cuts at: 113 113
Size: 4124
KasI G'GCGC_C
Cuts at: 1554 1802 1554
Size: 248 3876
KpnI G_GTAC'C
Cuts at: 1144 1144
Size: 4124
MluI A'CGCG_T
Cuts at: 3557 3557
Size: 4124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2795 2979 2795
Size: 184 3940
MscI TGG'CCA
Cuts at: 523 1885 523
Size: 1362 2762
MslI CAynn'nnrTG
Cuts at: 980 2113 2240 980
Size: 1133 127 2864
Fragments arranged by size:
2864 1133 127
MspA1I CmG'CkG
Cuts at: 1235 1549 1909 2922 3167 1235
Size: 314 360 1013 245 2192
Fragments arranged by size:
2192 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2305 2305
Size: 4124
NarI GG'CG_CC
Cuts at: 1555 1803 1555
Size: 248 3876
NgoMIV G'CCGG_C
Cuts at: 2303 2303
Size: 4124
NsiI A_TGCA'T
Cuts at: 977 977
Size: 4124
NspI r_CATG'y
Cuts at: 975 1177 1677 2584 975
Size: 202 500 907 2515
Fragments arranged by size:
2515 907 500 202
PasI CC'CwG_GG
Cuts at: 995 995
Size: 4124
PciI A'CATG_T
Cuts at: 2580 2580
Size: 4124
PmeI GTTT'AAAC
Cuts at: 1123 1123
Size: 4124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 4092
PpuMI rG'GwC_Cy
Cuts at: 880 1000 880
Size: 120 4004
PspOMI G'GGCC_C
Cuts at: 257 257
Size: 4124
PstI C_TGCA'G
Cuts at: 1171 1856 1171
Size: 685 3439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4124
PvuII CAG'CTG
Cuts at: 1549 1909 1549
Size: 360 3764
RsrII CG'GwC_CG
Cuts at: 2319 2319
Size: 4124
SacI G_AGCT'C
Cuts at: 755 1138 755
Size: 383 3741
SalI G'TCGA_C
Cuts at: 1161 1161
Size: 4124
SanDI GG'GwC_CC
Cuts at: 1000 1000
Size: 4124
SapI GCTCTTCn'nnn_
Cuts at: 2147 2357 2147
Size: 210 3914
SbfI CC_TGCA'GG
Cuts at: 1171 1171
Size: 4124
ScaI AGT'ACT
Cuts at: 846 3641 846
Size: 2795 1329
SfcI C'TryA_G
Cuts at: 33 1167 1852 2845 3036 33
Size: 1134 685 993 191 1121
Fragments arranged by size:
1134 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1556 1804 1556
Size: 248 3876
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
SmaI CCC'GGG
Cuts at: 767 1146 767
Size: 379 3745
SmlI C'TyrA_G
Cuts at: 456 885 1279 2194 2686 2948 456
Size: 429 394 915 492 262 1632
Fragments arranged by size:
1632 915 492 429 394 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4124
SphI G_CATG'C
Cuts at: 975 1177 1677 975
Size: 202 500 3422
Fragments arranged by size:
3422 500 202
StyI C'CwwG_G
Cuts at: 941 1005 1258 1612 941
Size: 64 253 354 3453
Fragments arranged by size:
3453 354 253 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1008 1707 1840 2299 2508 1008
Size: 699 133 459 209 2624
Fragments arranged by size:
2624 699 459 209 133
TatI w'GTAC_w
Cuts at: 844 3639 844
Size: 2795 1329
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 4092
Tth111I GACn'n_nGTC
Cuts at: 1921 1921
Size: 4124
XbaI T'CTAG_A
Cuts at: 172 1155 172
Size: 983 3141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1619 1619
Size: 4124
XmaI C'CCGG_G
Cuts at: 765 1144 765
Size: 379 3745
XmnI GAAnn'nnTTC
Cuts at: 168 168
Size: 4124
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PasI PciI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BsrGI BstXI BstZ17I Bsu36I BtgI ClaI DraIII
EcoRV FseI FspAI MfeI NcoI NdeI NheI NotI
NruI PacI PflMI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI