Restriction map for
pF1KB9631
(Circular) (Six-base) MAPSORT of: pF1KB9631.seq Check: 7079 from: 1 to: 3929
pF1KB9631 3929 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 447 967 447
Size: 520 3409
Acc65I G'GTAC_C
Cuts at: 945 945
Size: 3929
AclI AA'CG_TT
Cuts at: 3769 3769
Size: 3929
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1754 2186 2933 3201 472
Size: 1282 432 747 268 1200
Fragments arranged by size:
1282 1200 747 432 268
AflIII A'CryG_T
Cuts at: 152 1281 2385 3362 152
Size: 1129 1104 977 719
Fragments arranged by size:
1129 1104 977 719
AgeI A'CCGG_T
Cuts at: 2317 2317
Size: 3929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 905 905
Size: 3929
AlwNI CAG_nnn'CTG
Cuts at: 1234 2801 1234
Size: 1567 2362
ApaLI G'TGCA_C
Cuts at: 2699 2699
Size: 3929
ApoI r'AATT_y
Cuts at: 933 3282 3538 933
Size: 2349 256 1324
Fragments arranged by size:
2349 1324 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3929
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
AvaI C'yCGr_G
Cuts at: 380 398 535 687 949 380
Size: 18 137 152 262 3360
Fragments arranged by size:
3360 262 152 137 18
BamHI G'GATC_C
Cuts at: 954 954
Size: 3929
BanI G'GyrC_C
Cuts at: 371 403 694 945 1359 1607 1642 371
Size: 32 291 251 414 248 35 2658
Fragments arranged by size:
2658 414 291 251 248 35 32
BanII G_rGCy'C
Cuts at: 688 943 1973 688
Size: 255 1030 2644
Fragments arranged by size:
2644 1030 255
BbeI G_GCGC'C
Cuts at: 375 407 698 1363 1611 375
Size: 32 291 665 248 2693
Fragments arranged by size:
2693 665 291 248 32
BbsI GAAGACnn'nnnn_
Cuts at: 445 445
Size: 3929
BbvCI CC'TCA_GC
Cuts at: 278 278
Size: 3929
BciVI GTATCCnnnnn_n'
Cuts at: 1827 2588 3929 1827
Size: 761 1341 1827
Fragments arranged by size:
1827 1341 761
BclI T'GATC_A
Cuts at: 1449 1449
Size: 3929
BglI GCCn_nnn'nGGC
Cuts at: 190 339 412 692 835 190
Size: 149 73 280 143 3284
Fragments arranged by size:
3284 280 149 143 73
BglII A'GATC_T
Cuts at: 1444 3481 1444
Size: 2037 1892
BlpI GC'TnA_GC
Cuts at: 682 1041 682
Size: 359 3570
Bme1580I G_kGCm'C
Cuts at: 597 1554 1647 2703 597
Size: 957 93 1056 1823
Fragments arranged by size:
1823 1056 957 93
BmgBI CAC'GTC
Cuts at: 153 153
Size: 3929
BmrI ACTGGGnnnn_n'
Cuts at: 1141 1222 1557 3701 1141
Size: 81 335 2144 1369
Fragments arranged by size:
2144 1369 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 3929
Bpu10I CC'TnA_GC
Cuts at: 278 709 278
Size: 431 3498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1984 2476 2774 1105
Size: 879 492 298 2260
Fragments arranged by size:
2260 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 118 556 598 3633 118
Size: 438 42 3035 414
Fragments arranged by size:
3035 438 414 42
BsaAI yAC'GTr
Cuts at: 746 1282 746
Size: 536 3393
BsaBI GATnn'nnATC
Cuts at: 6 1443 3480 6
Size: 1437 2037 455
Fragments arranged by size:
2037 1437 455
BsaHI Gr'CG_yC
Cuts at: 372 404 542 695 1360 1608 2310 3806 372
Size: 32 138 153 665 248 702 1496 495
Fragments arranged by size:
1496 702 665 495 248 153 138 32
BsaWI w'CCGG_w
Cuts at: 1118 1639 2317 2591 2738 3149 1118
Size: 521 678 274 147 411 1898
Fragments arranged by size:
1898 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 884 914 884
Size: 30 3899
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 247 250 768 244
Size: 3 3 518 3405
Fragments arranged by size:
3405 518 3 3
BseYI C'CCAG_C
Cuts at: 345 1354 2112 2689 3185 345
Size: 1009 758 577 496 1089
Fragments arranged by size:
1089 1009 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 798 445
Size: 353 3576
BsiEI CG_ry'CG
Cuts at: 104 416 839 1471 1517 2725 104
Size: 312 423 632 46 1208 1308
Fragments arranged by size:
1308 1208 632 423 312 46
BsiHKAI G_wGCw'C
Cuts at: 524 943 1721 1911 2703 524
Size: 419 778 190 792 1750
Fragments arranged by size:
1750 792 778 419 190
BsmBI CGTCTCn'nnnn_
Cuts at: 399 412 399
Size: 13 3916
Bsp1286I G_dGCh'C
Cuts at: 524 597 688 943 1554 1647 1721 1911 1973
Size: 73 91 255 611 93 74 190 62
Cuts at: 1973 2703 524
Size: 730 1750
Fragments arranged by size:
1750 730 611 255 190 93 91 74
73 62
BspEI T'CCGG_A
Cuts at: 3149 3149
Size: 3929
BspHI T'CATG_A
Cuts at: 3105 3105
Size: 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 979 1193 1495 1876 979
Size: 214 302 381 3032
Fragments arranged by size:
3032 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2221 2275 3759 3924 50
Size: 2171 54 1484 165 55
Fragments arranged by size:
2171 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1841 1841
Size: 3929
BsrFI r'CCGG_y
Cuts at: 329 1927 2108 2317 329
Size: 1598 181 209 1941
Fragments arranged by size:
1941 1598 209 181
BssSI C'ACGA_G
Cuts at: 2200 2558 2200
Size: 358 3571
BstAPI GCAn_nnn'nTGC
Cuts at: 1306 1306
Size: 3929
BstBI TT'CG_AA
Cuts at: 2290 2290
Size: 3929
BstYI r'GATC_y
Cuts at: 1 954 1421 1444 1779 2025 3037 3123 3135
Size: 953 467 23 335 246 1012 86 12
Cuts at: 3135 3481 1
Size: 346 449
Fragments arranged by size:
1012 953 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1879 2060 2223 1879
Size: 181 163 3585
Fragments arranged by size:
3585 181 163
BtsI GCAGTG_nn'
Cuts at: 624 680 1172 624
Size: 56 492 3381
Fragments arranged by size:
3381 492 56
DraI TTT'AAA
Cuts at: 928 928
Size: 3929
DrdI GACnn_nn'nnGTC
Cuts at: 204 1635 2493 204
Size: 1431 858 1640
Fragments arranged by size:
1640 1431 858
EaeI y'GGCC_r
Cuts at: 413 836 1514 1688 2079 2106 3451 413
Size: 423 678 174 391 27 1345 891
Fragments arranged by size:
1345 891 678 423 391 174 27
EagI C'GGCC_G
Cuts at: 413 836 1514 413
Size: 423 678 2828
Fragments arranged by size:
2828 678 423
EarI CTCTTCn'nnn_
Cuts at: 140 221 667 1952 2162 140
Size: 81 446 1285 210 1907
Fragments arranged by size:
1907 1285 446 210 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 634 2137 2447 2593 3739 634
Size: 1503 310 146 1146 824
Fragments arranged by size:
1503 1146 824 310 146
EcoICRI GAG'CTC
Cuts at: 941 941
Size: 3929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1754 2186 2933 3201 472
Size: 1282 432 747 268 1200
Fragments arranged by size:
1282 1200 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 645 1068 645
Size: 423 3506
EcoRI G'AATT_C
Cuts at: 933 933
Size: 3929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3929
FspI TGC'GCA
Cuts at: 1710 1710
Size: 3929
HaeII r_GCGC'y
Cuts at: 218 375 407 661 698 704 1363 1611 2633
Size: 157 32 254 37 6 659 248 1022
Cuts at: 2633 3612 218
Size: 979 535
Fragments arranged by size:
1022 979 659 535 254 248 157 37
32 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 715 747 1771 1801 1803 1833 715
Size: 32 1024 30 2 30 2811
Fragments arranged by size:
2811 1024 32 30 30 2
HincII GTy'rAC
Cuts at: 968 968
Size: 3929
KasI G'GCGC_C
Cuts at: 371 403 694 1359 1607 371
Size: 32 291 665 248 2693
Fragments arranged by size:
2693 665 291 248 32
KpnI G_GTAC'C
Cuts at: 949 949
Size: 3929
MluI A'CGCG_T
Cuts at: 3362 3362
Size: 3929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2600 2784 2600
Size: 184 3745
MscI TGG'CCA
Cuts at: 1690 1690
Size: 3929
MslI CAynn'nnrTG
Cuts at: 887 1918 2045 887
Size: 1031 127 2771
Fragments arranged by size:
2771 1031 127
MspA1I CmG'CkG
Cuts at: 204 719 812 1040 1354 1714 2727 2972 204
Size: 515 93 228 314 360 1013 245 1161
Fragments arranged by size:
1161 1013 515 360 314 245 228 93
NaeI GCC'GGC
Cuts at: 331 2110 331
Size: 1779 2150
NarI GG'CG_CC
Cuts at: 372 404 695 1360 1608 372
Size: 32 291 665 248 2693
Fragments arranged by size:
2693 665 291 248 32
NgoMIV G'CCGG_C
Cuts at: 329 2108 329
Size: 1779 2150
NspI r_CATG'y
Cuts at: 483 982 1482 2389 483
Size: 499 500 907 2023
Fragments arranged by size:
2023 907 500 499
PciI A'CATG_T
Cuts at: 2385 2385
Size: 3929
PmeI GTTT'AAAC
Cuts at: 928 928
Size: 3929
PmlI CAC'GTG
Cuts at: 746 746
Size: 3929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3100 3068
Size: 32 3897
PstI C_TGCA'G
Cuts at: 976 1661 976
Size: 685 3244
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3929
PvuII CAG'CTG
Cuts at: 812 1354 1714 812
Size: 542 360 3027
Fragments arranged by size:
3027 542 360
RsrII CG'GwC_CG
Cuts at: 2124 2124
Size: 3929
SacI G_AGCT'C
Cuts at: 943 943
Size: 3929
SalI G'TCGA_C
Cuts at: 966 966
Size: 3929
SapI GCTCTTCn'nnn_
Cuts at: 667 1952 2162 667
Size: 1285 210 2434
Fragments arranged by size:
2434 1285 210
SbfI CC_TGCA'GG
Cuts at: 976 976
Size: 3929
ScaI AGT'ACT
Cuts at: 3446 3446
Size: 3929
SfcI C'TryA_G
Cuts at: 33 807 972 1657 2650 2841 33
Size: 774 165 685 993 191 1121
Fragments arranged by size:
1121 993 774 685 191 165
SfoI GGC'GCC
Cuts at: 373 405 696 1361 1609 373
Size: 32 291 665 248 2693
Fragments arranged by size:
2693 665 291 248 32
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
SmaI CCC'GGG
Cuts at: 382 689 951 382
Size: 307 262 3360
Fragments arranged by size:
3360 307 262
SmlI C'TyrA_G
Cuts at: 1084 1999 2491 2753 1084
Size: 915 492 262 2260
Fragments arranged by size:
2260 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3929
SphI G_CATG'C
Cuts at: 483 982 1482 483
Size: 499 500 2930
Fragments arranged by size:
2930 500 499
StyI C'CwwG_G
Cuts at: 128 185 1063 1417 128
Size: 57 878 354 2640
Fragments arranged by size:
2640 878 354 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1512 1645 2104 2313 1512
Size: 133 459 209 3128
Fragments arranged by size:
3128 459 209 133
TatI w'GTAC_w
Cuts at: 3444 3444
Size: 3929
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 884 916 3446 3478 884
Size: 32 2530 32 1335
Fragments arranged by size:
2530 1335 32 32
Tth111I GACn'n_nGTC
Cuts at: 1726 1726
Size: 3929
XbaI T'CTAG_A
Cuts at: 960 960
Size: 3929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1424 1424
Size: 3929
XmaI C'CCGG_G
Cuts at: 380 687 949 380
Size: 307 262 3360
Fragments arranged by size:
3360 307 262
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PmlI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmtI BpmI BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI