Restriction map for
pF1KB9633
(Circular) (Six-base) MAPSORT of: pF1KB9633.seq Check: 9473 from: 1 to: 4499
pF1KB9633 4499 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 390 1537 390
Size: 1147 3352
Acc65I G'GTAC_C
Cuts at: 1515 1515
Size: 4499
AclI AA'CG_TT
Cuts at: 4339 4339
Size: 4499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1466 2324 2756 3503 3771 1466
Size: 858 432 747 268 2194
Fragments arranged by size:
2194 858 747 432 268
AflIII A'CryG_T
Cuts at: 1851 2955 3932 1851
Size: 1104 977 2418
Fragments arranged by size:
2418 1104 977
AgeI A'CCGG_T
Cuts at: 2887 2887
Size: 4499
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1049 1049
Size: 4499
AlwNI CAG_nnn'CTG
Cuts at: 1489 1804 3371 1489
Size: 315 1567 2617
Fragments arranged by size:
2617 1567 315
ApaI G_GGCC'C
Cuts at: 1000 1000
Size: 4499
ApaLI G'TGCA_C
Cuts at: 3269 3269
Size: 4499
ApoI r'AATT_y
Cuts at: 301 855 1062 1138 1503 3852 4108 301
Size: 554 207 76 365 2349 256 692
Fragments arranged by size:
2349 692 554 365 256 207 76
AseI AT'TA_AT
Cuts at: 20 20
Size: 4499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 921 1519 921
Size: 598 3901
BamHI G'GATC_C
Cuts at: 1524 1524
Size: 4499
BanI G'GyrC_C
Cuts at: 1515 1929 2177 2212 1515
Size: 414 248 35 3802
Fragments arranged by size:
3802 414 248 35
BanII G_rGCy'C
Cuts at: 715 1000 1513 2543 715
Size: 285 513 1030 2671
Fragments arranged by size:
2671 1030 513 285
BbeI G_GCGC'C
Cuts at: 1933 2181 1933
Size: 248 4251
BbsI GAAGACnn'nnnn_
Cuts at: 224 980 224
Size: 756 3743
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4465
BciVI GTATCCnnnnn_n'
Cuts at: 2397 3158 4499 2397
Size: 761 1341 2397
Fragments arranged by size:
2397 1341 761
BclI T'GATC_A
Cuts at: 2019 2019
Size: 4499
BglII A'GATC_T
Cuts at: 373 2014 4051 373
Size: 1641 2037 821
Fragments arranged by size:
2037 1641 821
BlpI GC'TnA_GC
Cuts at: 458 1611 458
Size: 1153 3346
Bme1580I G_kGCm'C
Cuts at: 1000 1218 2124 2217 3273 1000
Size: 218 906 93 1056 2226
Fragments arranged by size:
2226 1056 906 218 93
BmrI ACTGGGnnnn_n'
Cuts at: 439 1711 1792 2127 4271 439
Size: 1272 81 335 2144 667
Fragments arranged by size:
2144 1272 667 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1458 2866 1458
Size: 1408 3091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 894 995 1367 894
Size: 101 372 4026
Fragments arranged by size:
4026 372 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 497 1006 1432 1675 2554 3046 3344 497
Size: 509 426 243 879 492 298 1652
Fragments arranged by size:
1652 879 509 492 426 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 1286 4203 1286
Size: 2917 1582
BsaAI yAC'GTr
Cuts at: 1852 1852
Size: 4499
BsaBI GATnn'nnATC
Cuts at: 6 2013 4050 6
Size: 2007 2037 455
Fragments arranged by size:
2037 2007 455
BsaHI Gr'CG_yC
Cuts at: 148 1930 2178 2880 4376 148
Size: 1782 248 702 1496 271
Fragments arranged by size:
1782 1496 702 271 248
BsaWI w'CCGG_w
Cuts at: 1088 1688 2209 2887 3161 3308 3719 1088
Size: 600 521 678 274 147 411 1868
Fragments arranged by size:
1868 678 600 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 133 163 892 922 1070 1100 133
Size: 30 729 30 148 30 3532
Fragments arranged by size:
3532 729 148 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 250 250
Size: 4499
BseYI C'CCAG_C
Cuts at: 1924 2682 3259 3755 1924
Size: 758 577 496 2668
Fragments arranged by size:
2668 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 739
Size: 4499
BsiEI CG_ry'CG
Cuts at: 104 2041 2087 3295 104
Size: 1937 46 1208 1308
Fragments arranged by size:
1937 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 605 715 1298 1513 2291 2481 3273 605
Size: 110 583 215 778 190 792 1831
Fragments arranged by size:
1831 792 778 583 215 190 110
BsmBI CGTCTCn'nnnn_
Cuts at: 155 155
Size: 4499
Bsp1286I G_dGCh'C
Cuts at: 605 715 1000 1218 1298 1513 2124 2217 2291
Size: 110 285 218 80 215 611 93 74
Cuts at: 2291 2481 2543 3273 605
Size: 190 62 730 1831
Fragments arranged by size:
1831 730 611 285 218 215 190 110
93 80 74 62
BspEI T'CCGG_A
Cuts at: 1088 3719 1088
Size: 2631 1868
BspHI T'CATG_A
Cuts at: 3675 3675
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1549 1763 2065 2446 1549
Size: 214 302 381 3602
Fragments arranged by size:
3602 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2791 2845 4329 4494 50
Size: 2741 54 1484 165 55
Fragments arranged by size:
2741 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 174 2411 174
Size: 2237 2262
BsrFI r'CCGG_y
Cuts at: 2497 2678 2887 2497
Size: 181 209 4109
Fragments arranged by size:
4109 209 181
BssSI C'ACGA_G
Cuts at: 377 2770 3128 377
Size: 2393 358 1748
Fragments arranged by size:
2393 1748 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1876 1876
Size: 4499
BstBI TT'CG_AA
Cuts at: 1196 2860 1196
Size: 1664 2835
BstYI r'GATC_y
Cuts at: 1 373 1524 1991 2014 2349 2595 3607 3693
Size: 372 1151 467 23 335 246 1012 86
Cuts at: 3693 3705 4051 1
Size: 12 346 449
Fragments arranged by size:
1151 1012 467 449 372 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2449 2630 2793 2449
Size: 181 163 4155
Fragments arranged by size:
4155 181 163
BtsI GCAGTG_nn'
Cuts at: 1742 1742
Size: 4499
DraI TTT'AAA
Cuts at: 452 1498 452
Size: 1046 3453
DrdI GACnn_nn'nnGTC
Cuts at: 2205 3063 2205
Size: 858 3641
EaeI y'GGCC_r
Cuts at: 2084 2258 2649 2676 4021 2084
Size: 174 391 27 1345 2562
Fragments arranged by size:
2562 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2084 2084
Size: 4499
EarI CTCTTCn'nnn_
Cuts at: 2522 2732 2522
Size: 210 4289
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 363 2707 3017 3163 4309 363
Size: 2344 310 146 1146 553
Fragments arranged by size:
2344 1146 553 310 146
EcoICRI GAG'CTC
Cuts at: 713 1511 713
Size: 798 3701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 894 995 1367 1466 2324 2756 3503 3771 894
Size: 101 372 99 858 432 747 268 1622
Fragments arranged by size:
1622 858 747 432 372 268 101 99
EcoO109I rG'GnC_Cy
Cuts at: 987 996 997 1638 987
Size: 9 1 641 3848
Fragments arranged by size:
3848 641 9 1
EcoRI G'AATT_C
Cuts at: 301 855 1062 1503 301
Size: 554 207 441 3297
Fragments arranged by size:
3297 554 441 207
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4499
FspI TGC'GCA
Cuts at: 2280 2280
Size: 4499
HaeII r_GCGC'y
Cuts at: 1933 2181 3203 4182 1933
Size: 248 1022 979 2250
Fragments arranged by size:
2250 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 133 165 802 834 1165 1197 2341 2371 2373
Size: 32 637 32 331 32 1144 30 2
Cuts at: 2373 2403 133
Size: 30 2229
Fragments arranged by size:
2229 1144 637 331 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 391 900 1538 391
Size: 509 638 3352
Fragments arranged by size:
3352 638 509
KasI G'GCGC_C
Cuts at: 1929 2177 1929
Size: 248 4251
KpnI G_GTAC'C
Cuts at: 1519 1519
Size: 4499
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 268 911 3170 3354 268
Size: 643 2259 184 1413
Fragments arranged by size:
2259 1413 643 184
MscI TGG'CCA
Cuts at: 2260 2260
Size: 4499
MslI CAynn'nnrTG
Cuts at: 1031 2488 2615 1031
Size: 1457 127 2915
Fragments arranged by size:
2915 1457 127
MspA1I CmG'CkG
Cuts at: 114 544 1610 1924 2284 3297 3542 114
Size: 430 1066 314 360 1013 245 1071
Fragments arranged by size:
1071 1066 1013 430 360 314 245
NaeI GCC'GGC
Cuts at: 2680 2680
Size: 4499
NarI GG'CG_CC
Cuts at: 1930 2178 1930
Size: 248 4251
NdeI CA'TA_TG
Cuts at: 507 507
Size: 4499
NgoMIV G'CCGG_C
Cuts at: 2678 2678
Size: 4499
NspI r_CATG'y
Cuts at: 287 1030 1036 1552 2052 2959 287
Size: 743 6 516 500 907 1827
Fragments arranged by size:
1827 907 743 516 500 6
PciI A'CATG_T
Cuts at: 2955 2955
Size: 4499
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4467
PpuMI rG'GwC_Cy
Cuts at: 987 987
Size: 4499
PspOMI G'GGCC_C
Cuts at: 996 996
Size: 4499
PstI C_TGCA'G
Cuts at: 733 1434 1546 2231 733
Size: 701 112 685 3001
Fragments arranged by size:
3001 701 685 112
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 114 544 1924 2284 114
Size: 430 1380 360 2329
Fragments arranged by size:
2329 1380 430 360
RsrII CG'GwC_CG
Cuts at: 2694 2694
Size: 4499
SacI G_AGCT'C
Cuts at: 715 1513 715
Size: 798 3701
SalI G'TCGA_C
Cuts at: 389 1536 389
Size: 1147 3352
SapI GCTCTTCn'nnn_
Cuts at: 2522 2732 2522
Size: 210 4289
SbfI CC_TGCA'GG
Cuts at: 1546 1546
Size: 4499
ScaI AGT'ACT
Cuts at: 4016 4016
Size: 4499
SexAI A'CCwGG_T
Cuts at: 401 401
Size: 4499
SfcI C'TryA_G
Cuts at: 33 539 729 1430 1542 2227 3220 3411 33
Size: 506 190 701 112 685 993 191 1121
Fragments arranged by size:
1121 993 701 685 506 191 190 112
SfoI GGC'GCC
Cuts at: 1931 2179 1931
Size: 248 4251
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 1521 1521
Size: 4499
SmlI C'TyrA_G
Cuts at: 512 1021 1447 1654 2569 3061 3323 512
Size: 509 426 207 915 492 262 1688
Fragments arranged by size:
1688 915 509 492 426 262 207
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 1036 1552 2052 1036
Size: 516 500 3483
Fragments arranged by size:
3483 516 500
StyI C'CwwG_G
Cuts at: 992 1633 1987 992
Size: 641 354 3504
Fragments arranged by size:
3504 641 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 827 2082 2215 2674 2883 827
Size: 1255 133 459 209 2443
Fragments arranged by size:
2443 1255 459 209 133
TatI w'GTAC_w
Cuts at: 548 4014 548
Size: 3466 1033
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1070 1102 4016 4048 1070
Size: 32 2914 32 1521
Fragments arranged by size:
2914 1521 32 32
Tth111I GACn'n_nGTC
Cuts at: 2296 2296
Size: 4499
XbaI T'CTAG_A
Cuts at: 1530 1530
Size: 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 168 1994 168
Size: 1826 2673
XmaI C'CCGG_G
Cuts at: 1519 1519
Size: 4499
XmnI GAAnn'nnTTC
Cuts at: 305 859 1336 305
Size: 554 477 3468
Fragments arranged by size:
3468 554 477
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI