Restriction map for
pF1KB9634
(Circular) (Six-base) MAPSORT of: pF1KB9634.seq Check: 8402 from: 1 to: 4088
pF1KB9634 4088 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1126 1126
Size: 4088
Acc65I G'GTAC_C
Cuts at: 984 1104 984
Size: 120 3968
AclI AA'CG_TT
Cuts at: 3928 3928
Size: 4088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 666 748 1913 2345 3092 3360 249
Size: 417 82 1165 432 747 268 977
Fragments arranged by size:
1165 977 747 432 417 268 82
AflIII A'CryG_T
Cuts at: 955 1440 2544 3521 955
Size: 485 1104 977 1522
Fragments arranged by size:
1522 1104 977 485
AgeI A'CCGG_T
Cuts at: 2476 2476
Size: 4088
AhdI GACnn_n'nnGTC
Cuts at: 1055 1055
Size: 4088
AlwNI CAG_nnn'CTG
Cuts at: 512 1393 2960 512
Size: 881 1567 1640
Fragments arranged by size:
1640 1567 881
ApaI G_GGCC'C
Cuts at: 656 885 656
Size: 229 3859
ApaLI G'TGCA_C
Cuts at: 2858 2858
Size: 4088
ApoI r'AATT_y
Cuts at: 1092 3441 3697 1092
Size: 2349 256 1483
Fragments arranged by size:
2349 1483 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4088
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
AvaI C'yCGr_G
Cuts at: 226 834 1108 226
Size: 608 274 3206
Fragments arranged by size:
3206 608 274
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 839 872 839
Size: 33 4055
BamHI G'GATC_C
Cuts at: 1113 1113
Size: 4088
BanI G'GyrC_C
Cuts at: 807 984 1104 1518 1766 1801 807
Size: 177 120 414 248 35 3094
Fragments arranged by size:
3094 414 248 177 120 35
BanII G_rGCy'C
Cuts at: 656 724 835 885 1102 2132 656
Size: 68 111 50 217 1030 2612
Fragments arranged by size:
2612 1030 217 111 68 50
BbeI G_GCGC'C
Cuts at: 1522 1770 1522
Size: 248 3840
BbvCI CC'TCA_GC
Cuts at: 345 381 345
Size: 36 4052
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4054
BciVI GTATCCnnnnn_n'
Cuts at: 127 833 840 1986 2747 4088 127
Size: 706 7 1146 761 1341 127
Fragments arranged by size:
1341 1146 761 706 127 7
BclI T'GATC_A
Cuts at: 1608 1608
Size: 4088
BglII A'GATC_T
Cuts at: 1603 3640 1603
Size: 2037 2051
BlpI GC'TnA_GC
Cuts at: 1200 1200
Size: 4088
Bme1580I G_kGCm'C
Cuts at: 656 885 1713 1806 2862 656
Size: 229 828 93 1056 1882
Fragments arranged by size:
1882 1056 828 229 93
BmrI ACTGGGnnnn_n'
Cuts at: 457 1300 1381 1716 3860 457
Size: 843 81 335 2144 685
Fragments arranged by size:
2144 843 685 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 4088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 650 468
Size: 182 3906
Bpu10I CC'TnA_GC
Cuts at: 345 381 454 345
Size: 36 73 3979
Fragments arranged by size:
3979 73 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1264 2143 2635 2933 847
Size: 417 879 492 298 2002
Fragments arranged by size:
2002 879 492 417 298
BsaI GGTCTCn'nnnn_
Cuts at: 1063 3792 1063
Size: 2729 1359
BsaAI yAC'GTr
Cuts at: 1441 1441
Size: 4088
BsaBI GATnn'nnATC
Cuts at: 6 1602 3639 6
Size: 1596 2037 455
Fragments arranged by size:
2037 1596 455
BsaHI Gr'CG_yC
Cuts at: 1519 1767 2469 3965 1519
Size: 248 702 1496 1642
Fragments arranged by size:
1642 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1277 1798 2476 2750 2897 3308 1277
Size: 521 678 274 147 411 2057
Fragments arranged by size:
2057 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 333 342 345 354 1064 333
Size: 9 3 9 710 3357
Fragments arranged by size:
3357 710 9 9 3
BseYI C'CCAG_C
Cuts at: 259 538 554 595 700 706 759 1513 2271
Size: 279 16 41 105 6 53 754 758
Cuts at: 2271 2848 3344 259
Size: 577 496 1003
Fragments arranged by size:
1003 758 754 577 496 279 105 53
41 16 6
BsiEI CG_ry'CG
Cuts at: 104 1630 1676 2884 104
Size: 1526 46 1208 1308
Fragments arranged by size:
1526 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1102 1880 2070 2862 1102
Size: 778 190 792 2328
Fragments arranged by size:
2328 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 656 724 835 885 1102 1713 1806 1880 2070
Size: 68 111 50 217 611 93 74 190
Cuts at: 2070 2132 2862 656
Size: 62 730 1882
Fragments arranged by size:
1882 730 611 217 190 111 93 74
68 62 50
BspEI T'CCGG_A
Cuts at: 3308 3308
Size: 4088
BspHI T'CATG_A
Cuts at: 3264 3264
Size: 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1138 1352 1654 2035 1138
Size: 214 302 381 3191
Fragments arranged by size:
3191 381 302 214
BsrBI CCG'CTC
Cuts at: 50 458 878 2380 2434 3918 4083 50
Size: 408 420 1502 54 1484 165 55
Fragments arranged by size:
1502 1484 420 408 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2000 2000
Size: 4088
BsrFI r'CCGG_y
Cuts at: 694 2086 2267 2476 694
Size: 1392 181 209 2306
Fragments arranged by size:
2306 1392 209 181
BssSI C'ACGA_G
Cuts at: 2359 2717 2359
Size: 358 3730
BstAPI GCAn_nnn'nTGC
Cuts at: 512 1465 512
Size: 953 3135
BstBI TT'CG_AA
Cuts at: 2449 2449
Size: 4088
BstXI CCAn_nnnn'nTGG
Cuts at: 216 943 216
Size: 727 3361
BstYI r'GATC_y
Cuts at: 1 1113 1580 1603 1938 2184 3196 3282 3294
Size: 1112 467 23 335 246 1012 86 12
Cuts at: 3294 3640 1
Size: 346 449
Fragments arranged by size:
1112 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 209 209
Size: 4088
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2038 2219 2382 2038
Size: 181 163 3744
Fragments arranged by size:
3744 181 163
BtsI GCAGTG_nn'
Cuts at: 248 1331 248
Size: 1083 3005
DraI TTT'AAA
Cuts at: 1087 1087
Size: 4088
DrdI GACnn_nn'nnGTC
Cuts at: 1794 2652 1794
Size: 858 3230
EaeI y'GGCC_r
Cuts at: 218 582 1673 1847 2238 2265 3610 218
Size: 364 1091 174 391 27 1345 696
Fragments arranged by size:
1345 1091 696 391 364 174 27
EagI C'GGCC_G
Cuts at: 1673 1673
Size: 4088
EarI CTCTTCn'nnn_
Cuts at: 2111 2321 2111
Size: 210 3878
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2296 2606 2752 3898 2296
Size: 310 146 1146 2486
Fragments arranged by size:
2486 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1100 1100
Size: 4088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 468 650 666 748 1913 2345 3092 3360
Size: 219 182 16 82 1165 432 747 268
Cuts at: 3360 249
Size: 977
Fragments arranged by size:
1165 977 747 432 268 219 182 82
16
EcoO109I rG'GnC_Cy
Cuts at: 154 474 653 881 882 1005 1049 1227 154
Size: 320 179 228 1 123 44 178 3015
Fragments arranged by size:
3015 320 228 179 178 123 44 1
EcoRI G'AATT_C
Cuts at: 1092 1092
Size: 4088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 993 2107 993
Size: 1114 2974
FspI TGC'GCA
Cuts at: 1869 1869
Size: 4088
HaeII r_GCGC'y
Cuts at: 1522 1770 2792 3771 1522
Size: 248 1022 979 1839
Fragments arranged by size:
1839 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 463 495 1041 1073 1930 1960 1962 1992 463
Size: 32 546 32 857 30 2 30 2559
Fragments arranged by size:
2559 857 546 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1127 1127
Size: 4088
KasI G'GCGC_C
Cuts at: 1518 1766 1518
Size: 248 3840
KpnI G_GTAC'C
Cuts at: 988 1108 988
Size: 120 3968
MluI A'CGCG_T
Cuts at: 3521 3521
Size: 4088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 995 2759 2943 995
Size: 1764 184 2140
Fragments arranged by size:
2140 1764 184
MscI TGG'CCA
Cuts at: 220 1849 220
Size: 1629 2459
MslI CAynn'nnrTG
Cuts at: 274 944 2077 2204 274
Size: 670 1133 127 2158
Fragments arranged by size:
2158 1133 670 127
MspA1I CmG'CkG
Cuts at: 241 318 636 759 905 1199 1513 1873 2886
Size: 77 318 123 146 294 314 360 1013
Cuts at: 2886 3131 241
Size: 245 1198
Fragments arranged by size:
1198 1013 360 318 314 294 245 146
123 77
NaeI GCC'GGC
Cuts at: 2269 2269
Size: 4088
NarI GG'CG_CC
Cuts at: 1519 1767 1519
Size: 248 3840
NcoI C'CATG_G
Cuts at: 209 209
Size: 4088
NgoMIV G'CCGG_C
Cuts at: 2267 2267
Size: 4088
NspI r_CATG'y
Cuts at: 959 1141 1641 2548 959
Size: 182 500 907 2499
Fragments arranged by size:
2499 907 500 182
PasI CC'CwG_GG
Cuts at: 566 1065 566
Size: 499 3589
PciI A'CATG_T
Cuts at: 955 2544 955
Size: 1589 2499
PflMI CCAn_nnn'nTGG
Cuts at: 759 759
Size: 4088
PfoI T'CCnGG_A
Cuts at: 150 150
Size: 4088
PmeI GTTT'AAAC
Cuts at: 1087 1087
Size: 4088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 4056
PpuMI rG'GwC_Cy
Cuts at: 154 1049 154
Size: 895 3193
PspOMI G'GGCC_C
Cuts at: 652 881 652
Size: 229 3859
PstI C_TGCA'G
Cuts at: 1135 1820 1135
Size: 685 3403
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4088
PvuII CAG'CTG
Cuts at: 318 636 759 1513 1873 318
Size: 318 123 754 360 2533
Fragments arranged by size:
2533 754 360 318 123
RsrII CG'GwC_CG
Cuts at: 2283 2283
Size: 4088
SacI G_AGCT'C
Cuts at: 1102 1102
Size: 4088
SalI G'TCGA_C
Cuts at: 1125 1125
Size: 4088
SanDI GG'GwC_CC
Cuts at: 154 154
Size: 4088
SapI GCTCTTCn'nnn_
Cuts at: 2111 2321 2111
Size: 210 3878
SbfI CC_TGCA'GG
Cuts at: 1135 1135
Size: 4088
ScaI AGT'ACT
Cuts at: 3605 3605
Size: 4088
SfcI C'TryA_G
Cuts at: 33 1131 1816 2809 3000 33
Size: 1098 685 993 191 1121
Fragments arranged by size:
1121 1098 993 685 191
SfoI GGC'GCC
Cuts at: 1520 1768 1520
Size: 248 3840
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
SmaI CCC'GGG
Cuts at: 1110 1110
Size: 4088
SmlI C'TyrA_G
Cuts at: 826 1243 2158 2650 2912 826
Size: 417 915 492 262 2002
Fragments arranged by size:
2002 915 492 417 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4088
SphI G_CATG'C
Cuts at: 1141 1641 1141
Size: 500 3588
StuI AGG'CCT
Cuts at: 614 614
Size: 4088
StyI C'CwwG_G
Cuts at: 209 866 1222 1576 209
Size: 657 356 354 2721
Fragments arranged by size:
2721 657 356 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 766 825 1671 1804 2263 2472 766
Size: 59 846 133 459 209 2382
Fragments arranged by size:
2382 846 459 209 133 59
TatI w'GTAC_w
Cuts at: 3603 3603
Size: 4088
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3605 3637 3605
Size: 32 4056
Tth111I GACn'n_nGTC
Cuts at: 1885 1885
Size: 4088
XbaI T'CTAG_A
Cuts at: 1119 1119
Size: 4088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 127 213 708 756 1583 127
Size: 86 495 48 827 2632
Fragments arranged by size:
2632 827 495 86 48
XmaI C'CCGG_G
Cuts at: 1108 1108
Size: 4088
XmnI GAAnn'nnTTC
Cuts at: 733 733
Size: 4088
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BbsI BfrBI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI