Restriction map for
pF1KB9635
(Circular) (Six-base) MAPSORT of: pF1KB9635.seq Check: 3938 from: 1 to: 4082
pF1KB9635 4082 bp
With 182 enzymes: SgfI *
June 25, 2015 11:41 ..
AccI GT'mk_AC
Cuts at: 361 1120 361
Size: 759 3323
Acc65I G'GTAC_C
Cuts at: 1098 1098
Size: 4082
AclI AA'CG_TT
Cuts at: 3922 3922
Size: 4082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1907 2339 3086 3354 1907
Size: 432 747 268 2635
Fragments arranged by size:
2635 747 432 268
AflIII A'CryG_T
Cuts at: 1025 1434 2538 3515 1025
Size: 409 1104 977 1592
Fragments arranged by size:
1592 1104 977 409
AgeI A'CCGG_T
Cuts at: 2470 2470
Size: 4082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 484 484
Size: 4082
AlwNI CAG_nnn'CTG
Cuts at: 432 793 1387 2954 432
Size: 361 594 1567 1560
Fragments arranged by size:
1567 1560 594 361
ApaLI G'TGCA_C
Cuts at: 757 2852 757
Size: 2095 1987
ApoI r'AATT_y
Cuts at: 1086 3435 3691 1086
Size: 2349 256 1477
Fragments arranged by size:
2349 1477 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4082
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
AvaI C'yCGr_G
Cuts at: 237 1102 237
Size: 865 3217
BamHI G'GATC_C
Cuts at: 841 1107 841
Size: 266 3816
BanI G'GyrC_C
Cuts at: 217 1005 1039 1098 1512 1760 1795 217
Size: 788 34 59 414 248 35 2504
Fragments arranged by size:
2504 788 414 248 59 35 34
BanII G_rGCy'C
Cuts at: 158 289 1096 2126 158
Size: 131 807 1030 2114
Fragments arranged by size:
2114 1030 807 131
BbeI G_GCGC'C
Cuts at: 1043 1516 1764 1043
Size: 473 248 3361
Fragments arranged by size:
3361 473 248
BciVI GTATCCnnnnn_n'
Cuts at: 1980 2741 4082 1980
Size: 761 1341 1980
Fragments arranged by size:
1980 1341 761
BclI T'GATC_A
Cuts at: 1602 1602
Size: 4082
BglI GCCn_nnn'nGGC
Cuts at: 790 790
Size: 4082
BglII A'GATC_T
Cuts at: 632 1597 3634 632
Size: 965 2037 1080
Fragments arranged by size:
2037 1080 965
BlpI GC'TnA_GC
Cuts at: 259 1194 259
Size: 935 3147
Bme1580I G_kGCm'C
Cuts at: 220 761 1707 1800 2856 220
Size: 541 946 93 1056 1446
Fragments arranged by size:
1446 1056 946 541 93
BmrI ACTGGGnnnn_n'
Cuts at: 221 978 1294 1375 1710 3854 221
Size: 757 316 81 335 2144 449
Fragments arranged by size:
2144 757 449 335 316 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 4082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 625
Size: 4082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 874 1258 2137 2629 2927 424
Size: 450 384 879 492 298 1579
Fragments arranged by size:
1579 879 492 450 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 3786 3786
Size: 4082
BsaAI yAC'GTr
Cuts at: 1026 1435 1026
Size: 409 3673
BsaBI GATnn'nnATC
Cuts at: 6 1596 3633 6
Size: 1590 2037 455
Fragments arranged by size:
2037 1590 455
BsaHI Gr'CG_yC
Cuts at: 119 1040 1070 1513 1761 2463 3959 119
Size: 921 30 443 248 702 1496 242
Fragments arranged by size:
1496 921 702 443 248 242 30
BsaWI w'CCGG_w
Cuts at: 1271 1792 2470 2744 2891 3302 1271
Size: 521 678 274 147 411 2051
Fragments arranged by size:
2051 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 622 622
Size: 4082
BseYI C'CCAG_C
Cuts at: 921 1507 2265 2842 3338 921
Size: 586 758 577 496 1665
Fragments arranged by size:
1665 758 586 577 496
BsiEI CG_ry'CG
Cuts at: 104 860 914 1624 1670 2878 104
Size: 756 54 710 46 1208 1308
Fragments arranged by size:
1308 1208 756 710 54 46
BsiHKAI G_wGCw'C
Cuts at: 761 1096 1874 2064 2856 761
Size: 335 778 190 792 1987
Fragments arranged by size:
1987 792 778 335 190
BsiWI C'GTAC_G
Cuts at: 515 515
Size: 4082
BsmBI CGTCTCn'nnnn_
Cuts at: 442 442
Size: 4082
Bsp1286I G_dGCh'C
Cuts at: 158 220 289 761 1096 1707 1800 1874 2064
Size: 62 69 472 335 611 93 74 190
Cuts at: 2064 2126 2856 158
Size: 62 730 1384
Fragments arranged by size:
1384 730 611 472 335 190 93 74
69 62 62
BspEI T'CCGG_A
Cuts at: 3302 3302
Size: 4082
BspHI T'CATG_A
Cuts at: 3258 3258
Size: 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1132 1346 1648 2029 1132
Size: 214 302 381 3185
Fragments arranged by size:
3185 381 302 214
BsrBI CCG'CTC
Cuts at: 50 953 2374 2428 3912 4077 50
Size: 903 1421 54 1484 165 55
Fragments arranged by size:
1484 1421 903 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1994 1994
Size: 4082
BsrFI r'CCGG_y
Cuts at: 174 1042 2080 2261 2470 174
Size: 868 1038 181 209 1786
Fragments arranged by size:
1786 1038 868 209 181
BssHII G'CGCG_C
Cuts at: 296 571 296
Size: 275 3807
BssSI C'ACGA_G
Cuts at: 2353 2711 2353
Size: 358 3724
BstAPI GCAn_nnn'nTGC
Cuts at: 432 793 983 998 1459 432
Size: 361 190 15 461 3055
Fragments arranged by size:
3055 461 361 190 15
BstBI TT'CG_AA
Cuts at: 2443 2443
Size: 4082
BstXI CCAn_nnnn'nTGG
Cuts at: 603 652 603
Size: 49 4033
BstYI r'GATC_y
Cuts at: 1 632 841 1107 1574 1597 1932 2178 3190
Size: 631 209 266 467 23 335 246 1012
Cuts at: 3190 3276 3288 3634 1
Size: 86 12 346 449
Fragments arranged by size:
1012 631 467 449 346 335 266 246
209 86 23 12
BtgI C'CryG_G
Cuts at: 107 488 752 812 908 107
Size: 381 264 60 96 3281
Fragments arranged by size:
3281 381 264 96 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 468 2032 2213 2376 468
Size: 1564 181 163 2174
Fragments arranged by size:
2174 1564 181 163
BtsI GCAGTG_nn'
Cuts at: 330 948 1325 330
Size: 618 377 3087
Fragments arranged by size:
3087 618 377
DraI TTT'AAA
Cuts at: 1081 1081
Size: 4082
DraIII CAC_nnn'GTG
Cuts at: 1026 1026
Size: 4082
DrdI GACnn_nn'nnGTC
Cuts at: 1788 2646 1788
Size: 858 3224
EaeI y'GGCC_r
Cuts at: 911 1667 1841 2232 2259 3604 911
Size: 756 174 391 27 1345 1389
Fragments arranged by size:
1389 1345 756 391 174 27
EagI C'GGCC_G
Cuts at: 911 1667 911
Size: 756 3326
EarI CTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2290 2600 2746 3892 2290
Size: 310 146 1146 2480
Fragments arranged by size:
2480 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1094 1094
Size: 4082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 1907 2339 3086 3354 625
Size: 1282 432 747 268 1353
Fragments arranged by size:
1353 1282 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 502 502
Size: 4082
EcoO109I rG'GnC_Cy
Cuts at: 1221 1221
Size: 4082
EcoRI G'AATT_C
Cuts at: 1086 1086
Size: 4082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 4082
FspI TGC'GCA
Cuts at: 538 1863 538
Size: 1325 2757
HaeII r_GCGC'y
Cuts at: 206 266 281 830 1043 1516 1764 2786 3765
Size: 60 15 549 213 473 248 1022 979
Cuts at: 3765 206
Size: 523
Fragments arranged by size:
1022 979 549 523 473 248 213 60
15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1924 1954 1956 1986 1924
Size: 30 2 30 4020
Fragments arranged by size:
4020 30 30 2
HincII GTy'rAC
Cuts at: 1121 1121
Size: 4082
HindIII A'AGCT_T
Cuts at: 541 541
Size: 4082
KasI G'GCGC_C
Cuts at: 1039 1512 1760 1039
Size: 473 248 3361
Fragments arranged by size:
3361 473 248
KpnI G_GTAC'C
Cuts at: 1102 1102
Size: 4082
MluI A'CGCG_T
Cuts at: 3515 3515
Size: 4082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1091 2753 2937 1091
Size: 1662 184 2236
Fragments arranged by size:
2236 1662 184
MscI TGG'CCA
Cuts at: 1843 1843
Size: 4082
MslI CAynn'nnrTG
Cuts at: 487 586 2071 2198 487
Size: 99 1485 127 2371
Fragments arranged by size:
2371 1485 127 99
MspA1I CmG'CkG
Cuts at: 197 432 754 790 805 814 910 921 1193
Size: 235 322 36 15 9 96 11 272
Cuts at: 1193 1507 1867 2880 3125 197
Size: 314 360 1013 245 1154
Fragments arranged by size:
1154 1013 360 322 314 272 245 235
96 36 15 11 9
NaeI GCC'GGC
Cuts at: 176 1044 2263 176
Size: 868 1219 1995
Fragments arranged by size:
1995 1219 868
NarI GG'CG_CC
Cuts at: 1040 1513 1761 1040
Size: 473 248 3361
Fragments arranged by size:
3361 473 248
NcoI C'CATG_G
Cuts at: 107 488 107
Size: 381 3701
NgoMIV G'CCGG_C
Cuts at: 174 1042 2261 174
Size: 868 1219 1995
Fragments arranged by size:
1995 1219 868
NotI GC'GGCC_GC
Cuts at: 911 911
Size: 4082
NspI r_CATG'y
Cuts at: 471 981 999 1135 1635 2542 471
Size: 510 18 136 500 907 2011
Fragments arranged by size:
2011 907 510 500 136 18
PciI A'CATG_T
Cuts at: 2538 2538
Size: 4082
PmeI GTTT'AAAC
Cuts at: 1081 1081
Size: 4082
PmlI CAC'GTG
Cuts at: 1026 1026
Size: 4082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 4050
PstI C_TGCA'G
Cuts at: 428 810 994 1129 1814 428
Size: 382 184 135 685 2696
Fragments arranged by size:
2696 685 382 184 135
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4082
PvuII CAG'CTG
Cuts at: 432 1507 1867 432
Size: 1075 360 2647
Fragments arranged by size:
2647 1075 360
RsrII CG'GwC_CG
Cuts at: 2277 2277
Size: 4082
SacI G_AGCT'C
Cuts at: 1096 1096
Size: 4082
SacII CC_GC'GG
Cuts at: 755 815 911 755
Size: 60 96 3926
Fragments arranged by size:
3926 96 60
SalI G'TCGA_C
Cuts at: 1119 1119
Size: 4082
SapI GCTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
SbfI CC_TGCA'GG
Cuts at: 1129 1129
Size: 4082
ScaI AGT'ACT
Cuts at: 3599 3599
Size: 4082
SfcI C'TryA_G
Cuts at: 33 424 806 990 1125 1810 2803 2994 33
Size: 391 382 184 135 685 993 191 1121
Fragments arranged by size:
1121 993 685 391 382 191 184 135
SfoI GGC'GCC
Cuts at: 1041 1514 1762 1041
Size: 473 248 3361
Fragments arranged by size:
3361 473 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
SmaI CCC'GGG
Cuts at: 1104 1104
Size: 4082
SmlI C'TyrA_G
Cuts at: 439 889 1237 2152 2644 2906 439
Size: 450 348 915 492 262 1615
Fragments arranged by size:
1615 915 492 450 348 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4082
SphI G_CATG'C
Cuts at: 471 981 1135 1635 471
Size: 510 154 500 2918
Fragments arranged by size:
2918 510 500 154
StyI C'CwwG_G
Cuts at: 107 488 1073 1216 1570 107
Size: 381 585 143 354 2619
Fragments arranged by size:
2619 585 381 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 846 1665 1798 2257 2466 846
Size: 819 133 459 209 2462
Fragments arranged by size:
2462 819 459 209 133
TatI w'GTAC_w
Cuts at: 3597 3597
Size: 4082
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4050
Tth111I GACn'n_nGTC
Cuts at: 1879 1879
Size: 4082
XbaI T'CTAG_A
Cuts at: 1113 1113
Size: 4082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 564 1577 564
Size: 1013 3069
XmaI C'CCGG_G
Cuts at: 1102 1102
Size: 4082
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PciI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsgI BsmI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NruI NsiI PacI PasI PflMI PfoI
PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI