Restriction map for
pF1KB9641
(Circular) (Six-base) MAPSORT of: pF1KB9641.seq Check: 5278 from: 1 to: 4079
pF1KB9641 4079 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1117 1117
Size: 4079
Acc65I G'GTAC_C
Cuts at: 1095 1095
Size: 4079
AclI AA'CG_TT
Cuts at: 3919 3919
Size: 4079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 1904 2336 3083 3351 385
Size: 1519 432 747 268 1113
Fragments arranged by size:
1519 1113 747 432 268
AfeI AGC'GCT
Cuts at: 322 796 322
Size: 474 3605
AflIII A'CryG_T
Cuts at: 1431 2535 3512 1431
Size: 1104 977 1998
Fragments arranged by size:
1998 1104 977
AgeI A'CCGG_T
Cuts at: 2467 2467
Size: 4079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 158 158
Size: 4079
AlwNI CAG_nnn'CTG
Cuts at: 288 1384 2951 288
Size: 1096 1567 1416
Fragments arranged by size:
1567 1416 1096
ApaI G_GGCC'C
Cuts at: 258 484 258
Size: 226 3853
ApaLI G'TGCA_C
Cuts at: 2849 2849
Size: 4079
ApoI r'AATT_y
Cuts at: 1083 3432 3688 1083
Size: 2349 256 1474
Fragments arranged by size:
2349 1474 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4079
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
AvaI C'yCGr_G
Cuts at: 183 763 1099 183
Size: 580 336 3163
Fragments arranged by size:
3163 580 336
BamHI G'GATC_C
Cuts at: 1104 1104
Size: 4079
BanI G'GyrC_C
Cuts at: 173 769 1095 1509 1757 1792 173
Size: 596 326 414 248 35 2460
Fragments arranged by size:
2460 596 414 326 248 35
BanII G_rGCy'C
Cuts at: 190 258 484 652 692 817 1093 2123 190
Size: 68 226 168 40 125 276 1030 2146
Fragments arranged by size:
2146 1030 276 226 168 125 68 40
BbeI G_GCGC'C
Cuts at: 1513 1761 1513
Size: 248 3831
BciVI GTATCCnnnnn_n'
Cuts at: 1977 2738 4079 1977
Size: 761 1341 1977
Fragments arranged by size:
1977 1341 761
BclI T'GATC_A
Cuts at: 1599 1599
Size: 4079
BglII A'GATC_T
Cuts at: 1594 3631 1594
Size: 2037 2042
BlpI GC'TnA_GC
Cuts at: 1191 1191
Size: 4079
Bme1580I G_kGCm'C
Cuts at: 176 258 484 772 887 1704 1797 2853 176
Size: 82 226 288 115 817 93 1056 1402
Fragments arranged by size:
1402 1056 817 288 226 115 93 82
BmgBI CAC'GTC
Cuts at: 336 336
Size: 4079
BmrI ACTGGGnnnn_n'
Cuts at: 1291 1372 1707 3851 1291
Size: 81 335 2144 1519
Fragments arranged by size:
2144 1519 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 309 789 2446 309
Size: 480 1657 1942
Fragments arranged by size:
1942 1657 480
Bpu10I CC'TnA_GC
Cuts at: 189 189
Size: 4079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1255 2134 2626 2924 568
Size: 687 879 492 298 1723
Fragments arranged by size:
1723 879 687 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1015 3783 1015
Size: 2768 1311
BsaAI yAC'GTr
Cuts at: 1432 1432
Size: 4079
BsaBI GATnn'nnATC
Cuts at: 6 1593 3630 6
Size: 1587 2037 455
Fragments arranged by size:
2037 1587 455
BsaHI Gr'CG_yC
Cuts at: 1510 1758 2460 3956 1510
Size: 248 702 1496 1633
Fragments arranged by size:
1633 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1268 1789 2467 2741 2888 3299 1268
Size: 521 678 274 147 411 2048
Fragments arranged by size:
2048 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 757 760 949 1033 757
Size: 3 189 84 3803
Fragments arranged by size:
3803 189 84 3
BseYI C'CCAG_C
Cuts at: 150 477 484 1504 2262 2839 3335 150
Size: 327 7 1020 758 577 496 894
Fragments arranged by size:
1020 894 758 577 496 327 7
BsiEI CG_ry'CG
Cuts at: 104 603 755 1621 1667 2875 104
Size: 499 152 866 46 1208 1308
Fragments arranged by size:
1308 1208 866 499 152 46
BsiHKAI G_wGCw'C
Cuts at: 467 1093 1871 2061 2853 467
Size: 626 778 190 792 1693
Fragments arranged by size:
1693 792 778 626 190
BsmBI CGTCTCn'nnnn_
Cuts at: 271 271
Size: 4079
Bsp1286I G_dGCh'C
Cuts at: 176 190 258 467 484 652 692 772 817
Size: 14 68 209 17 168 40 80 45
Cuts at: 817 887 1093 1704 1797 1871 2061 2123 2853
Size: 70 206 611 93 74 190 62 730
Cuts at: 2853 176
Size: 1402
Fragments arranged by size:
1402 730 611 209 206 190 168 93
80 74 70 68 62 45 40 17
14
BspEI T'CCGG_A
Cuts at: 3299 3299
Size: 4079
BspHI T'CATG_A
Cuts at: 3255 3255
Size: 4079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1129 1343 1645 2026 1129
Size: 214 302 381 3182
Fragments arranged by size:
3182 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2371 2425 3909 4074 50
Size: 2321 54 1484 165 55
Fragments arranged by size:
2321 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 668 1991 668
Size: 1323 2756
BsrFI r'CCGG_y
Cuts at: 750 2077 2258 2467 750
Size: 1327 181 209 2362
Fragments arranged by size:
2362 1327 209 181
BssSI C'ACGA_G
Cuts at: 274 2350 2708 274
Size: 2076 358 1645
Fragments arranged by size:
2076 1645 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1456 1456
Size: 4079
BstBI TT'CG_AA
Cuts at: 2440 2440
Size: 4079
BstXI CCAn_nnnn'nTGG
Cuts at: 303 423 303
Size: 120 3959
BstYI r'GATC_y
Cuts at: 1 579 1104 1571 1594 1929 2175 3187 3273
Size: 578 525 467 23 335 246 1012 86
Cuts at: 3273 3285 3631 1
Size: 12 346 449
Fragments arranged by size:
1012 578 525 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 428 107
Size: 321 3758
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2029 2210 2373 2029
Size: 181 163 3735
Fragments arranged by size:
3735 181 163
BtsI GCAGTG_nn'
Cuts at: 240 1322 240
Size: 1082 2997
DraI TTT'AAA
Cuts at: 1078 1078
Size: 4079
DrdI GACnn_nn'nnGTC
Cuts at: 1785 2643 1785
Size: 858 3221
EaeI y'GGCC_r
Cuts at: 425 600 713 752 1664 1838 2229 2256 3601
Size: 175 113 39 912 174 391 27 1345
Cuts at: 3601 425
Size: 903
Fragments arranged by size:
1345 912 903 391 175 174 113 39
27
EagI C'GGCC_G
Cuts at: 600 752 1664 600
Size: 152 912 3015
Fragments arranged by size:
3015 912 152
EarI CTCTTCn'nnn_
Cuts at: 2102 2312 2102
Size: 210 3869
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 607 2287 2597 2743 3889 127
Size: 480 1680 310 146 1146 317
Fragments arranged by size:
1680 1146 480 317 310 146
EcoICRI GAG'CTC
Cuts at: 1091 1091
Size: 4079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 1904 2336 3083 3351 385
Size: 1519 432 747 268 1113
Fragments arranged by size:
1519 1113 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 505 505
Size: 4079
EcoO109I rG'GnC_Cy
Cuts at: 254 255 481 637 1218 254
Size: 1 226 156 581 3115
Fragments arranged by size:
3115 581 226 156 1
EcoRI G'AATT_C
Cuts at: 1083 1083
Size: 4079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 375 816 2098 375
Size: 441 1282 2356
Fragments arranged by size:
2356 1282 441
FspI TGC'GCA
Cuts at: 613 1860 613
Size: 1247 2832
HaeII r_GCGC'y
Cuts at: 151 324 535 798 1513 1761 2783 3762 151
Size: 173 211 263 715 248 1022 979 468
Fragments arranged by size:
1022 979 715 468 263 248 211 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 799 831 1921 1951 1953 1983 799
Size: 32 1090 30 2 30 2895
Fragments arranged by size:
2895 1090 32 30 30 2
HincII GTy'rAC
Cuts at: 630 1118 630
Size: 488 3591
KasI G'GCGC_C
Cuts at: 1509 1757 1509
Size: 248 3831
KpnI G_GTAC'C
Cuts at: 1099 1099
Size: 4079
MluI A'CGCG_T
Cuts at: 3512 3512
Size: 4079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 842 2750 2934 842
Size: 1908 184 1987
Fragments arranged by size:
1987 1908 184
MscI TGG'CCA
Cuts at: 715 1840 715
Size: 1125 2954
MslI CAynn'nnrTG
Cuts at: 421 880 2068 2195 421
Size: 459 1188 127 2305
Fragments arranged by size:
2305 1188 459 127
MspA1I CmG'CkG
Cuts at: 125 477 1030 1190 1504 1864 2877 3122 125
Size: 352 553 160 314 360 1013 245 1082
Fragments arranged by size:
1082 1013 553 360 352 314 245 160
NaeI GCC'GGC
Cuts at: 752 2260 752
Size: 1508 2571
NarI GG'CG_CC
Cuts at: 1510 1758 1510
Size: 248 3831
NcoI C'CATG_G
Cuts at: 107 107
Size: 4079
NgoMIV G'CCGG_C
Cuts at: 750 2258 750
Size: 1508 2571
NotI GC'GGCC_GC
Cuts at: 600 600
Size: 4079
NspI r_CATG'y
Cuts at: 1132 1632 2539 1132
Size: 500 907 2672
Fragments arranged by size:
2672 907 500
PasI CC'CwG_GG
Cuts at: 168 259 168
Size: 91 3988
PciI A'CATG_T
Cuts at: 2535 2535
Size: 4079
PmeI GTTT'AAAC
Cuts at: 1078 1078
Size: 4079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 4047
PspOMI G'GGCC_C
Cuts at: 254 480 254
Size: 226 3853
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 553 585 553
Size: 32 4047
PstI C_TGCA'G
Cuts at: 272 1049 1126 1811 272
Size: 777 77 685 2540
Fragments arranged by size:
2540 777 685 77
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4079
PvuII CAG'CTG
Cuts at: 477 1504 1864 477
Size: 1027 360 2692
Fragments arranged by size:
2692 1027 360
RsrII CG'GwC_CG
Cuts at: 114 2274 114
Size: 2160 1919
SacI G_AGCT'C
Cuts at: 1093 1093
Size: 4079
SalI G'TCGA_C
Cuts at: 1116 1116
Size: 4079
SapI GCTCTTCn'nnn_
Cuts at: 2102 2312 2102
Size: 210 3869
SbfI CC_TGCA'GG
Cuts at: 1126 1126
Size: 4079
ScaI AGT'ACT
Cuts at: 571 928 3596 571
Size: 357 2668 1054
Fragments arranged by size:
2668 1054 357
SfcI C'TryA_G
Cuts at: 33 268 1045 1122 1807 2800 2991 33
Size: 235 777 77 685 993 191 1121
Fragments arranged by size:
1121 993 777 685 235 191 77
SfoI GGC'GCC
Cuts at: 1511 1759 1511
Size: 248 3831
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
SmaI CCC'GGG
Cuts at: 1101 1101
Size: 4079
SmlI C'TyrA_G
Cuts at: 583 1234 2149 2641 2903 583
Size: 651 915 492 262 1759
Fragments arranged by size:
1759 915 651 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4079
SphI G_CATG'C
Cuts at: 1132 1632 1132
Size: 500 3579
StyI C'CwwG_G
Cuts at: 107 350 1213 1567 107
Size: 243 863 354 2619
Fragments arranged by size:
2619 863 354 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 131 1662 1795 2254 2463 131
Size: 1531 133 459 209 1747
Fragments arranged by size:
1747 1531 459 209 133
TatI w'GTAC_w
Cuts at: 569 926 3594 569
Size: 357 2668 1054
Fragments arranged by size:
2668 1054 357
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 4047
Tth111I GACn'n_nGTC
Cuts at: 1876 1876
Size: 4079
XbaI T'CTAG_A
Cuts at: 1110 1110
Size: 4079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1574 1574
Size: 4079
XmaI C'CCGG_G
Cuts at: 1099 1099
Size: 4079
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PmeI PpiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI BpmI
BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PflMI
PfoI PmlI PpuMI PshAI PsiI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI