Restriction map for
pF1KB9642
(Circular) (Six-base) MAPSORT of: pF1KB9642.seq Check: 5610 from: 1 to: 4892
pF1KB9642 4892 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1342 1342
Size: 4892
AccI GT'mk_AC
Cuts at: 1930 1930
Size: 4892
Acc65I G'GTAC_C
Cuts at: 1908 1908
Size: 4892
AclI AA'CG_TT
Cuts at: 4732 4732
Size: 4892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1099 1315 2717 3149 3896 4164 1099
Size: 216 1402 432 747 268 1827
Fragments arranged by size:
1827 1402 747 432 268 216
AfeI AGC'GCT
Cuts at: 673 673
Size: 4892
AflIII A'CryG_T
Cuts at: 2244 3348 4325 2244
Size: 1104 977 2811
Fragments arranged by size:
2811 1104 977
AgeI A'CCGG_T
Cuts at: 3280 3280
Size: 4892
AleI CACnn'nnGTG
Cuts at: 1744 1744
Size: 4892
AlwNI CAG_nnn'CTG
Cuts at: 588 694 2197 3764 588
Size: 106 1503 1567 1716
Fragments arranged by size:
1716 1567 1503 106
ApaI G_GGCC'C
Cuts at: 777 777
Size: 4892
ApaLI G'TGCA_C
Cuts at: 3662 3662
Size: 4892
ApoI r'AATT_y
Cuts at: 1896 4245 4501 1896
Size: 2349 256 2287
Fragments arranged by size:
2349 2287 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4892
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
AvaI C'yCGr_G
Cuts at: 1912 1912
Size: 4892
BamHI G'GATC_C
Cuts at: 1667 1917 1667
Size: 250 4642
BanI G'GyrC_C
Cuts at: 906 1827 1908 2322 2570 2605 906
Size: 921 81 414 248 35 3193
Fragments arranged by size:
3193 921 414 248 81 35
BanII G_rGCy'C
Cuts at: 299 332 777 863 904 1906 2936 299
Size: 33 445 86 41 1002 1030 2255
Fragments arranged by size:
2255 1030 1002 445 86 41 33
BbeI G_GCGC'C
Cuts at: 2326 2574 2326
Size: 248 4644
BbsI GAAGACnn'nnnn_
Cuts at: 480 480
Size: 4892
BbvCI CC'TCA_GC
Cuts at: 379 682 931 379
Size: 303 249 4340
Fragments arranged by size:
4340 303 249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 442 476 442
Size: 34 4858
BciVI GTATCCnnnnn_n'
Cuts at: 2790 3551 4892 2790
Size: 761 1341 2790
Fragments arranged by size:
2790 1341 761
BclI T'GATC_A
Cuts at: 2412 2412
Size: 4892
BglI GCCn_nnn'nGGC
Cuts at: 147 1323 147
Size: 1176 3716
BglII A'GATC_T
Cuts at: 2407 4444 2407
Size: 2037 2855
BlpI GC'TnA_GC
Cuts at: 994 2004 994
Size: 1010 3882
Bme1580I G_kGCm'C
Cuts at: 777 911 1328 2517 2610 3666 777
Size: 134 417 1189 93 1056 2003
Fragments arranged by size:
2003 1189 1056 417 134 93
BmrI ACTGGGnnnn_n'
Cuts at: 2104 2185 2520 4664 2104
Size: 81 335 2144 2332
Fragments arranged by size:
2332 2144 335 81
BmtI G_CTAG'C
Cuts at: 646 646
Size: 4892
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1539 3259 1539
Size: 1720 3172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 280 293 316 349 565 610 688 826
Size: 80 13 23 33 216 45 78 138
Cuts at: 826 850 997 1130 1304 1436 1534 200
Size: 24 147 133 174 132 98 3558
Fragments arranged by size:
3558 216 174 147 138 133 132 98
80 78 45 33 24 23 13
Bpu10I CC'TnA_GC
Cuts at: 355 379 682 931 355
Size: 24 303 249 4316
Fragments arranged by size:
4316 303 249 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 781 1185 2068 2947 3439 3737 424
Size: 357 404 883 879 492 298 1579
Fragments arranged by size:
1579 883 879 492 404 357 298
BsaI GGTCTCn'nnnn_
Cuts at: 1418 4596 1418
Size: 3178 1714
BsaAI yAC'GTr
Cuts at: 2245 2245
Size: 4892
BsaBI GATnn'nnATC
Cuts at: 6 2406 4443 6
Size: 2400 2037 455
Fragments arranged by size:
2400 2037 455
BsaHI Gr'CG_yC
Cuts at: 2323 2571 3273 4769 2323
Size: 248 702 1496 2446
Fragments arranged by size:
2446 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2081 2602 3280 3554 3701 4112 2081
Size: 521 678 274 147 411 2861
Fragments arranged by size:
2861 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 205 235 205
Size: 30 4862
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 160 1195 1399 157
Size: 3 1035 204 3650
Fragments arranged by size:
3650 1035 204 3
BseYI C'CCAG_C
Cuts at: 224 769 786 981 1428 1470 1480 1576 2317
Size: 545 17 195 447 42 10 96 741
Cuts at: 2317 3075 3652 4148 224
Size: 758 577 496 968
Fragments arranged by size:
968 758 741 577 545 496 447 195
96 42 17 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 1549 201
Size: 1348 3544
BsiEI CG_ry'CG
Cuts at: 104 2434 2480 3688 104
Size: 2330 46 1208 1308
Fragments arranged by size:
2330 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 242 299 658 904 1906 2684 2874 3666 242
Size: 57 359 246 1002 778 190 792 1468
Fragments arranged by size:
1468 1002 792 778 359 246 190 57
BsmI GAATG_Cn'
Cuts at: 347 347
Size: 4892
BsmBI CGTCTCn'nnnn_
Cuts at: 718 718
Size: 4892
Bsp1286I G_dGCh'C
Cuts at: 242 299 332 658 777 863 904 911 1328
Size: 57 33 326 119 86 41 7 417
Cuts at: 1328 1906 2517 2610 2684 2874 2936 3666 242
Size: 578 611 93 74 190 62 730 1468
Fragments arranged by size:
1468 730 611 578 417 326 190 119
93 86 74 62 57 41 33 7
BspEI T'CCGG_A
Cuts at: 4112 4112
Size: 4892
BspHI T'CATG_A
Cuts at: 4068 4068
Size: 4892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1342 1942 2156 2458 2839 1342
Size: 600 214 302 381 3395
Fragments arranged by size:
3395 600 381 302 214
BsrBI CCG'CTC
Cuts at: 50 156 188 1523 3184 3238 4722 4887 50
Size: 106 32 1335 1661 54 1484 165 55
Fragments arranged by size:
1661 1484 1335 165 106 55 54 32
BsrDI GCAATG_nn'
Cuts at: 1049 1376 1575 2804 1049
Size: 327 199 1229 3137
Fragments arranged by size:
3137 1229 327 199
BsrFI r'CCGG_y
Cuts at: 2890 3071 3280 2890
Size: 181 209 4502
Fragments arranged by size:
4502 209 181
BssSI C'ACGA_G
Cuts at: 3163 3521 3163
Size: 358 4534
BstAPI GCAn_nnn'nTGC
Cuts at: 342 2269 342
Size: 1927 2965
BstBI TT'CG_AA
Cuts at: 3253 3253
Size: 4892
BstEII G'GTnAC_C
Cuts at: 1158 1861 1158
Size: 703 4189
BstXI CCAn_nnnn'nTGG
Cuts at: 1713 1713
Size: 4892
BstYI r'GATC_y
Cuts at: 1 809 951 1517 1667 1917 2384 2407 2742
Size: 808 142 566 150 250 467 23 335
Cuts at: 2742 2988 4000 4086 4098 4444 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 808 566 467 449 346 335 250
246 150 142 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 445 733 940 1591 445
Size: 288 207 651 3746
Fragments arranged by size:
3746 651 288 207
BtgI C'CryG_G
Cuts at: 107 145 420 1068 1706 107
Size: 38 275 648 638 3293
Fragments arranged by size:
3293 648 638 275 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2842 3023 3186 2842
Size: 181 163 4548
Fragments arranged by size:
4548 181 163
BtsI GCAGTG_nn'
Cuts at: 1871 2135 1871
Size: 264 4628
DraI TTT'AAA
Cuts at: 1891 1891
Size: 4892
DrdI GACnn_nn'nnGTC
Cuts at: 1156 2598 3456 1156
Size: 1442 858 2592
Fragments arranged by size:
2592 1442 858
EaeI y'GGCC_r
Cuts at: 246 820 878 1703 2477 2651 3042 3069 4414
Size: 574 58 825 774 174 391 27 1345
Cuts at: 4414 246
Size: 724
Fragments arranged by size:
1345 825 774 724 574 391 174 58
27
EagI C'GGCC_G
Cuts at: 2477 2477
Size: 4892
EarI CTCTTCn'nnn_
Cuts at: 550 1282 1888 2915 3125 550
Size: 732 606 1027 210 2317
Fragments arranged by size:
2317 1027 732 606 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 514 1213 3100 3410 3556 4702 514
Size: 699 1887 310 146 1146 704
Fragments arranged by size:
1887 1146 704 699 310 146
EcoICRI GAG'CTC
Cuts at: 297 902 1904 297
Size: 605 1002 3285
Fragments arranged by size:
3285 1002 605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 280 293 316 349 565 610 688 826
Size: 80 13 23 33 216 45 78 138
Cuts at: 826 850 997 1099 1130 1304 1315 1436 1534
Size: 24 147 102 31 174 11 121 98
Cuts at: 1534 2717 3149 3896 4164 200
Size: 1183 432 747 268 928
Fragments arranged by size:
1183 928 747 432 268 216 174 147
138 121 102 98 80 78 45 33
31 24 23 13 11
EcoNI CCTnn'n_nnAGG
Cuts at: 364 1449 364
Size: 1085 3807
EcoO109I rG'GnC_Cy
Cuts at: 413 478 629 773 774 945 1363 1494 1547
Size: 65 151 144 1 171 418 131 53
Cuts at: 1547 1595 1675 2031 413
Size: 48 80 356 3274
Fragments arranged by size:
3274 418 356 171 151 144 131 80
65 53 48 1
EcoRI G'AATT_C
Cuts at: 1896 1896
Size: 4892
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2911 2911
Size: 4892
FspI TGC'GCA
Cuts at: 2673 2673
Size: 4892
HaeII r_GCGC'y
Cuts at: 675 2326 2574 3596 4575 675
Size: 1651 248 1022 979 992
Fragments arranged by size:
1651 1022 992 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 1440 1472 1478 1510 1837 1869 2734
Size: 32 1203 32 6 32 327 32 865
Cuts at: 2734 2764 2766 2796 205
Size: 30 2 30 2301
Fragments arranged by size:
2301 1203 865 327 32 32 32 32
30 30 6 2
HincII GTy'rAC
Cuts at: 1931 1931
Size: 4892
KasI G'GCGC_C
Cuts at: 2322 2570 2322
Size: 248 4644
KpnI G_GTAC'C
Cuts at: 1912 1912
Size: 4892
MluI A'CGCG_T
Cuts at: 4325 4325
Size: 4892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3563 3747 3563
Size: 184 4708
MscI TGG'CCA
Cuts at: 248 822 880 1705 2653 248
Size: 574 58 825 948 2487
Fragments arranged by size:
2487 948 825 574 58
MslI CAynn'nnrTG
Cuts at: 1615 1657 1744 2881 3008 1615
Size: 42 87 1137 127 3499
Fragments arranged by size:
3499 1137 127 87 42
MspA1I CmG'CkG
Cuts at: 197 769 2003 2317 2677 3690 3935 197
Size: 572 1234 314 360 1013 245 1154
Fragments arranged by size:
1234 1154 1013 572 360 314 245
NaeI GCC'GGC
Cuts at: 3073 3073
Size: 4892
NarI GG'CG_CC
Cuts at: 2323 2571 2323
Size: 248 4644
NcoI C'CATG_G
Cuts at: 107 145 420 1068 1706 107
Size: 38 275 648 638 3293
Fragments arranged by size:
3293 648 638 275 38
NgoMIV G'CCGG_C
Cuts at: 3071 3071
Size: 4892
NheI G'CTAG_C
Cuts at: 642 642
Size: 4892
NspI r_CATG'y
Cuts at: 1040 1248 1399 1945 2445 3352 1040
Size: 208 151 546 500 907 2580
Fragments arranged by size:
2580 907 546 500 208 151
PasI CC'CwG_GG
Cuts at: 844 1672 844
Size: 828 4064
PciI A'CATG_T
Cuts at: 3348 3348
Size: 4892
PflMI CCAn_nnn'nTGG
Cuts at: 372 588 1092 372
Size: 216 504 4172
Fragments arranged by size:
4172 504 216
PfoI T'CCnGG_A
Cuts at: 299 365 947 1013 1091 1444 299
Size: 66 582 66 78 353 3747
Fragments arranged by size:
3747 582 353 78 66 66
PmeI GTTT'AAAC
Cuts at: 1891 1891
Size: 4892
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 4031
Size: 32 4860
PpuMI rG'GwC_Cy
Cuts at: 945 1494 1595 945
Size: 549 101 4242
Fragments arranged by size:
4242 549 101
PspOMI G'GGCC_C
Cuts at: 773 773
Size: 4892
PstI C_TGCA'G
Cuts at: 455 1939 2624 455
Size: 1484 685 2723
Fragments arranged by size:
2723 1484 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4892
PvuII CAG'CTG
Cuts at: 197 769 2317 2677 197
Size: 572 1548 360 2412
Fragments arranged by size:
2412 1548 572 360
RsrII CG'GwC_CG
Cuts at: 3087 3087
Size: 4892
SacI G_AGCT'C
Cuts at: 299 904 1906 299
Size: 605 1002 3285
Fragments arranged by size:
3285 1002 605
SalI G'TCGA_C
Cuts at: 1929 1929
Size: 4892
SapI GCTCTTCn'nnn_
Cuts at: 1282 1888 2915 3125 1282
Size: 606 1027 210 3049
Fragments arranged by size:
3049 1027 606 210
SbfI CC_TGCA'GG
Cuts at: 1939 1939
Size: 4892
ScaI AGT'ACT
Cuts at: 4409 4409
Size: 4892
SexAI A'CCwGG_T
Cuts at: 608 608
Size: 4892
SfcI C'TryA_G
Cuts at: 33 451 1935 2620 3613 3804 33
Size: 418 1484 685 993 191 1121
Fragments arranged by size:
1484 1121 993 685 418 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 147 147
Size: 4892
SfoI GGC'GCC
Cuts at: 2324 2572 2324
Size: 248 4644
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
SmaI CCC'GGG
Cuts at: 1914 1914
Size: 4892
SmlI C'TyrA_G
Cuts at: 403 796 1164 2047 2962 3454 3716 403
Size: 393 368 883 915 492 262 1579
Fragments arranged by size:
1579 915 883 492 393 368 262
SpeI A'CTAG_T
Cuts at: 67 723 67
Size: 656 4236
SphI G_CATG'C
Cuts at: 1040 1399 1945 2445 1040
Size: 359 546 500 3487
Fragments arranged by size:
3487 546 500 359
StuI AGG'CCT
Cuts at: 497 1455 497
Size: 958 3934
StyI C'CwwG_G
Cuts at: 107 145 420 1068 1706 2026 2380 107
Size: 38 275 648 638 320 354 2619
Fragments arranged by size:
2619 648 638 354 320 275 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2475 2608 3067 3276 2475
Size: 133 459 209 4091
Fragments arranged by size:
4091 459 209 133
TatI w'GTAC_w
Cuts at: 4407 4407
Size: 4892
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1502 1534 4409 4441 1502
Size: 32 2875 32 1953
Fragments arranged by size:
2875 1953 32 32
Tth111I GACn'n_nGTC
Cuts at: 2689 2689
Size: 4892
XbaI T'CTAG_A
Cuts at: 1923 1923
Size: 4892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 134 1066 1237 2387 134
Size: 932 171 1150 2639
Fragments arranged by size:
2639 1150 932 171
XmaI C'CCGG_G
Cuts at: 1912 1912
Size: 4892
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BfrBI BmgBI BsiWI BsrGI BssHII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI