Restriction map for
pF1KB9644
(Circular) (Six-base) MAPSORT of: pF1KB9644.seq Check: 9742 from: 1 to: 3650
pF1KB9644 3650 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 688 688
Size: 3650
Acc65I G'GTAC_C
Cuts at: 666 666
Size: 3650
AclI AA'CG_TT
Cuts at: 3490 3490
Size: 3650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 395 1475 1907 2654 2922 302
Size: 93 1080 432 747 268 1030
Fragments arranged by size:
1080 1030 747 432 268 93
AflIII A'CryG_T
Cuts at: 1002 2106 3083 1002
Size: 1104 977 1569
Fragments arranged by size:
1569 1104 977
AgeI A'CCGG_T
Cuts at: 2038 2038
Size: 3650
AleI CACnn'nnGTG
Cuts at: 628 628
Size: 3650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 646 646
Size: 3650
AlwNI CAG_nnn'CTG
Cuts at: 199 516 955 2522 199
Size: 317 439 1567 1327
Fragments arranged by size:
1567 1327 439 317
ApaI G_GGCC'C
Cuts at: 341 606 341
Size: 265 3385
ApaLI G'TGCA_C
Cuts at: 2420 2420
Size: 3650
ApoI r'AATT_y
Cuts at: 654 3003 3259 654
Size: 2349 256 1045
Fragments arranged by size:
2349 1045 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3650
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
AvaI C'yCGr_G
Cuts at: 299 334 418 473 589 599 670 299
Size: 35 84 55 116 10 71 3279
Fragments arranged by size:
3279 116 84 71 55 35 10
BamHI G'GATC_C
Cuts at: 611 675 611
Size: 64 3586
BanI G'GyrC_C
Cuts at: 156 666 1080 1328 1363 156
Size: 510 414 248 35 2443
Fragments arranged by size:
2443 510 414 248 35
BanII G_rGCy'C
Cuts at: 193 220 341 606 664 1694 193
Size: 27 121 265 58 1030 2149
Fragments arranged by size:
2149 1030 265 121 58 27
BbeI G_GCGC'C
Cuts at: 1084 1332 1084
Size: 248 3402
BciVI GTATCCnnnnn_n'
Cuts at: 1548 2309 3650 1548
Size: 761 1341 1548
Fragments arranged by size:
1548 1341 761
BclI T'GATC_A
Cuts at: 1170 1170
Size: 3650
BglI GCCn_nnn'nGGC
Cuts at: 112 316 336 401 112
Size: 204 20 65 3361
Fragments arranged by size:
3361 204 65 20
BglII A'GATC_T
Cuts at: 1165 3202 1165
Size: 2037 1613
BlpI GC'TnA_GC
Cuts at: 192 762 192
Size: 570 3080
Bme1580I G_kGCm'C
Cuts at: 341 606 1275 1368 2424 341
Size: 265 669 93 1056 1567
Fragments arranged by size:
1567 1056 669 265 93
BmrI ACTGGGnnnn_n'
Cuts at: 149 862 943 1278 3422 149
Size: 713 81 335 2144 377
Fragments arranged by size:
2144 713 377 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 343
Size: 3650
Bpu10I CC'TnA_GC
Cuts at: 200 251 200
Size: 51 3599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1705 2197 2495 826
Size: 879 492 298 1981
Fragments arranged by size:
1981 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3354 3354
Size: 3650
BsaAI yAC'GTr
Cuts at: 1003 1003
Size: 3650
BsaBI GATnn'nnATC
Cuts at: 6 1164 3201 6
Size: 1158 2037 455
Fragments arranged by size:
2037 1158 455
BsaHI Gr'CG_yC
Cuts at: 1081 1329 2031 3527 1081
Size: 248 702 1496 1204
Fragments arranged by size:
1496 1204 702 248
BsaWI w'CCGG_w
Cuts at: 162 839 1360 2038 2312 2459 2870 162
Size: 677 521 678 274 147 411 942
Fragments arranged by size:
942 678 677 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 210 210
Size: 3650
BseYI C'CCAG_C
Cuts at: 1075 1833 2410 2906 1075
Size: 758 577 496 1819
Fragments arranged by size:
1819 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1192 1238 2446 104
Size: 1088 46 1208 1308
Fragments arranged by size:
1308 1208 1088 46
BsiHKAI G_wGCw'C
Cuts at: 193 664 1442 1632 2424 193
Size: 471 778 190 792 1419
Fragments arranged by size:
1419 792 778 471 190
BsmBI CGTCTCn'nnnn_
Cuts at: 415 415
Size: 3650
Bsp1286I G_dGCh'C
Cuts at: 193 220 341 606 664 1275 1368 1442 1632
Size: 27 121 265 58 611 93 74 190
Cuts at: 1632 1694 2424 193
Size: 62 730 1419
Fragments arranged by size:
1419 730 611 265 190 121 93 74
62 58 27
BspEI T'CCGG_A
Cuts at: 2870 2870
Size: 3650
BspHI T'CATG_A
Cuts at: 2826 2826
Size: 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 700 914 1216 1597 700
Size: 214 302 381 2753
Fragments arranged by size:
2753 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1942 1996 3480 3645 50
Size: 1892 54 1484 165 55
Fragments arranged by size:
1892 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1562 1562
Size: 3650
BsrFI r'CCGG_y
Cuts at: 181 400 1648 1829 2038 181
Size: 219 1248 181 209 1793
Fragments arranged by size:
1793 1248 219 209 181
BssHII G'CGCG_C
Cuts at: 379 379
Size: 3650
BssSI C'ACGA_G
Cuts at: 1921 2279 1921
Size: 358 3292
BstAPI GCAn_nnn'nTGC
Cuts at: 1027 1027
Size: 3650
BstBI TT'CG_AA
Cuts at: 2011 2011
Size: 3650
BstYI r'GATC_y
Cuts at: 1 611 675 1142 1165 1500 1746 2758 2844
Size: 610 64 467 23 335 246 1012 86
Cuts at: 2844 2856 3202 1
Size: 12 346 449
Fragments arranged by size:
1012 610 467 449 346 335 246 86
64 23 12
Bsu36I CC'TnA_GG
Cuts at: 362 362
Size: 3650
BtgI C'CryG_G
Cuts at: 107 149 272 314 107
Size: 42 123 42 3443
Fragments arranged by size:
3443 123 42 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1600 1781 1944 1600
Size: 181 163 3306
Fragments arranged by size:
3306 181 163
BtsI GCAGTG_nn'
Cuts at: 893 893
Size: 3650
DraI TTT'AAA
Cuts at: 649 649
Size: 3650
DrdI GACnn_nn'nnGTC
Cuts at: 1356 2214 1356
Size: 858 2792
EaeI y'GGCC_r
Cuts at: 134 1235 1409 1800 1827 3172 134
Size: 1101 174 391 27 1345 612
Fragments arranged by size:
1345 1101 612 391 174 27
EagI C'GGCC_G
Cuts at: 1235 1235
Size: 3650
EarI CTCTTCn'nnn_
Cuts at: 1673 1883 1673
Size: 210 3440
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 418 604 624 1858 2168 2314 3460 418
Size: 186 20 1234 310 146 1146 608
Fragments arranged by size:
1234 1146 608 310 186 146 20
EcoICRI GAG'CTC
Cuts at: 191 662 191
Size: 471 3179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 343 395 1475 1907 2654 2922 302
Size: 41 52 1080 432 747 268 1030
Fragments arranged by size:
1080 1030 747 432 268 52 41
EcoNI CCTnn'n_nnAGG
Cuts at: 589 589
Size: 3650
EcoO109I rG'GnC_Cy
Cuts at: 394 469 789 394
Size: 75 320 3255
Fragments arranged by size:
3255 320 75
EcoRI G'AATT_C
Cuts at: 654 654
Size: 3650
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1669 1669
Size: 3650
FspI TGC'GCA
Cuts at: 634 1431 634
Size: 797 2853
FspAI rTGC'GCAy
Cuts at: 634 634
Size: 3650
HaeII r_GCGC'y
Cuts at: 361 1084 1332 2354 3333 361
Size: 723 248 1022 979 678
Fragments arranged by size:
1022 979 723 678 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1492 1522 1524 1554 1492
Size: 30 2 30 3588
Fragments arranged by size:
3588 30 30 2
HincII GTy'rAC
Cuts at: 689 689
Size: 3650
KasI G'GCGC_C
Cuts at: 1080 1328 1080
Size: 248 3402
KpnI G_GTAC'C
Cuts at: 670 670
Size: 3650
MluI A'CGCG_T
Cuts at: 3083 3083
Size: 3650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2321 2505 2321
Size: 184 3466
MscI TGG'CCA
Cuts at: 1411 1411
Size: 3650
MslI CAynn'nnrTG
Cuts at: 628 1639 1766 628
Size: 1011 127 2512
Fragments arranged by size:
2512 1011 127
MspA1I CmG'CkG
Cuts at: 151 274 316 492 573 761 1075 1435 2448
Size: 123 42 176 81 188 314 360 1013
Cuts at: 2448 2693 151
Size: 245 1108
Fragments arranged by size:
1108 1013 360 314 245 188 176 123
81 42
NaeI GCC'GGC
Cuts at: 402 1831 402
Size: 1429 2221
NarI GG'CG_CC
Cuts at: 1081 1329 1081
Size: 248 3402
NcoI C'CATG_G
Cuts at: 107 107
Size: 3650
NgoMIV G'CCGG_C
Cuts at: 400 1829 400
Size: 1429 2221
NspI r_CATG'y
Cuts at: 703 1203 2110 703
Size: 500 907 2243
Fragments arranged by size:
2243 907 500
PciI A'CATG_T
Cuts at: 2106 2106
Size: 3650
PmeI GTTT'AAAC
Cuts at: 649 649
Size: 3650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2821 2789
Size: 32 3618
PpuMI rG'GwC_Cy
Cuts at: 469 469
Size: 3650
PspOMI G'GGCC_C
Cuts at: 337 602 337
Size: 265 3385
PstI C_TGCA'G
Cuts at: 697 1382 697
Size: 685 2965
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3650
PvuII CAG'CTG
Cuts at: 492 573 1075 1435 492
Size: 81 502 360 2707
Fragments arranged by size:
2707 502 360 81
RsrII CG'GwC_CG
Cuts at: 1845 1845
Size: 3650
SacI G_AGCT'C
Cuts at: 193 664 193
Size: 471 3179
SacII CC_GC'GG
Cuts at: 152 275 317 152
Size: 123 42 3485
Fragments arranged by size:
3485 123 42
SalI G'TCGA_C
Cuts at: 687 687
Size: 3650
SanDI GG'GwC_CC
Cuts at: 469 469
Size: 3650
SapI GCTCTTCn'nnn_
Cuts at: 1673 1883 1673
Size: 210 3440
SbfI CC_TGCA'GG
Cuts at: 697 697
Size: 3650
ScaI AGT'ACT
Cuts at: 3167 3167
Size: 3650
SfcI C'TryA_G
Cuts at: 33 693 1378 2371 2562 33
Size: 660 685 993 191 1121
Fragments arranged by size:
1121 993 685 660 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 316 316
Size: 3650
SfoI GGC'GCC
Cuts at: 1082 1330 1082
Size: 248 3402
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
SmaI CCC'GGG
Cuts at: 475 672 475
Size: 197 3453
SmlI C'TyrA_G
Cuts at: 805 1720 2212 2474 805
Size: 915 492 262 1981
Fragments arranged by size:
1981 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3650
SphI G_CATG'C
Cuts at: 703 1203 703
Size: 500 3150
StuI AGG'CCT
Cuts at: 235 235
Size: 3650
StyI C'CwwG_G
Cuts at: 107 536 784 1138 107
Size: 429 248 354 2619
Fragments arranged by size:
2619 429 354 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 403 1233 1366 1825 2034 403
Size: 830 133 459 209 2019
Fragments arranged by size:
2019 830 459 209 133
TatI w'GTAC_w
Cuts at: 3165 3165
Size: 3650
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 129 161 3167 3199 129
Size: 32 3006 32 580
Fragments arranged by size:
3006 580 32 32
Tth111I GACn'n_nGTC
Cuts at: 413 1447 413
Size: 1034 2616
XbaI T'CTAG_A
Cuts at: 406 681 406
Size: 275 3375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1145 1145
Size: 3650
XmaI C'CCGG_G
Cuts at: 473 670 473
Size: 197 3453
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsrGI BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoRV FseI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspXI PsrI SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI