Restriction map for
pF1KB9646
(Circular) (Six-base) MAPSORT of: pF1KB9646.seq Check: 7856 from: 1 to: 3725
pF1KB9646 3725 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 763 763
Size: 3725
Acc65I G'GTAC_C
Cuts at: 741 741
Size: 3725
AclI AA'CG_TT
Cuts at: 3565 3565
Size: 3725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 1550 1982 2729 2997 434
Size: 1116 432 747 268 1162
Fragments arranged by size:
1162 1116 747 432 268
AflIII A'CryG_T
Cuts at: 1077 2181 3158 1077
Size: 1104 977 1644
Fragments arranged by size:
1644 1104 977
AgeI A'CCGG_T
Cuts at: 2113 2113
Size: 3725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 134 592 134
Size: 458 3267
AlwNI CAG_nnn'CTG
Cuts at: 707 1030 2597 707
Size: 323 1567 1835
Fragments arranged by size:
1835 1567 323
ApaLI G'TGCA_C
Cuts at: 2495 2495
Size: 3725
ApoI r'AATT_y
Cuts at: 729 3078 3334 729
Size: 2349 256 1120
Fragments arranged by size:
2349 1120 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3725
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
AvaI C'yCGr_G
Cuts at: 745 745
Size: 3725
BamHI G'GATC_C
Cuts at: 750 750
Size: 3725
BanI G'GyrC_C
Cuts at: 741 1155 1403 1438 741
Size: 414 248 35 3028
Fragments arranged by size:
3028 414 248 35
BanII G_rGCy'C
Cuts at: 739 1769 739
Size: 1030 2695
BbeI G_GCGC'C
Cuts at: 1159 1407 1159
Size: 248 3477
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 501 535 90
Size: 34 377 34 3280
Fragments arranged by size:
3280 377 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 385 1623 2384 3725 385
Size: 1238 761 1341 385
Fragments arranged by size:
1341 1238 761 385
BclI T'GATC_A
Cuts at: 1245 1245
Size: 3725
BglII A'GATC_T
Cuts at: 1240 3277 1240
Size: 2037 1688
BlpI GC'TnA_GC
Cuts at: 837 837
Size: 3725
Bme1580I G_kGCm'C
Cuts at: 1350 1443 2499 1350
Size: 93 1056 2576
Fragments arranged by size:
2576 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 937 1018 1353 3497 937
Size: 81 335 2144 1165
Fragments arranged by size:
2144 1165 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 3725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 901 1780 2272 2570 612
Size: 289 879 492 298 1767
Fragments arranged by size:
1767 879 492 298 289
BsaI GGTCTCn'nnnn_
Cuts at: 323 3429 323
Size: 3106 619
BsaAI yAC'GTr
Cuts at: 1078 1078
Size: 3725
BsaBI GATnn'nnATC
Cuts at: 6 1239 3276 6
Size: 1233 2037 455
Fragments arranged by size:
2037 1233 455
BsaHI Gr'CG_yC
Cuts at: 1156 1404 2106 3602 1156
Size: 248 702 1496 1279
Fragments arranged by size:
1496 1279 702 248
BsaWI w'CCGG_w
Cuts at: 191 914 1435 2113 2387 2534 2945 191
Size: 723 521 678 274 147 411 971
Fragments arranged by size:
971 723 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 387 1150 1908 2485 2981 387
Size: 763 758 577 496 1131
Fragments arranged by size:
1131 763 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1267 1313 2521 104
Size: 1163 46 1208 1308
Fragments arranged by size:
1308 1208 1163 46
BsiHKAI G_wGCw'C
Cuts at: 641 739 1517 1707 2499 641
Size: 98 778 190 792 1867
Fragments arranged by size:
1867 792 778 190 98
BsmI GAATG_Cn'
Cuts at: 304 365 304
Size: 61 3664
Bsp1286I G_dGCh'C
Cuts at: 641 739 1350 1443 1517 1707 1769 2499 641
Size: 98 611 93 74 190 62 730 1867
Fragments arranged by size:
1867 730 611 190 98 93 74 62
BspEI T'CCGG_A
Cuts at: 191 2945 191
Size: 2754 971
BspHI T'CATG_A
Cuts at: 2901 2901
Size: 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 775 989 1291 1672 775
Size: 214 302 381 2828
Fragments arranged by size:
2828 381 302 214
BsrBI CCG'CTC
Cuts at: 50 187 2017 2071 3555 3720 50
Size: 137 1830 54 1484 165 55
Fragments arranged by size:
1830 1484 165 137 55 54
BsrDI GCAATG_nn'
Cuts at: 1637 1637
Size: 3725
BsrFI r'CCGG_y
Cuts at: 1723 1904 2113 1723
Size: 181 209 3335
Fragments arranged by size:
3335 209 181
BsrGI T'GTAC_A
Cuts at: 596 596
Size: 3725
BssSI C'ACGA_G
Cuts at: 1996 2354 1996
Size: 358 3367
BstAPI GCAn_nnn'nTGC
Cuts at: 1102 1102
Size: 3725
BstBI TT'CG_AA
Cuts at: 2086 2086
Size: 3725
BstYI r'GATC_y
Cuts at: 1 549 750 1217 1240 1575 1821 2833 2919
Size: 548 201 467 23 335 246 1012 86
Cuts at: 2919 2931 3277 1
Size: 12 346 449
Fragments arranged by size:
1012 548 467 449 346 335 246 201
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 164 1675 1856 2019 164
Size: 1511 181 163 1870
Fragments arranged by size:
1870 1511 181 163
BtsI GCAGTG_nn'
Cuts at: 968 968
Size: 3725
DraI TTT'AAA
Cuts at: 724 724
Size: 3725
DrdI GACnn_nn'nnGTC
Cuts at: 1431 2289 1431
Size: 858 2867
EaeI y'GGCC_r
Cuts at: 427 1310 1484 1875 1902 3247 427
Size: 883 174 391 27 1345 905
Fragments arranged by size:
1345 905 883 391 174 27
EagI C'GGCC_G
Cuts at: 1310 1310
Size: 3725
EarI CTCTTCn'nnn_
Cuts at: 207 1748 1958 207
Size: 1541 210 1974
Fragments arranged by size:
1974 1541 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1933 2243 2389 3535 1933
Size: 310 146 1146 2123
Fragments arranged by size:
2123 1146 310 146
EcoICRI GAG'CTC
Cuts at: 737 737
Size: 3725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 1550 1982 2729 2997 434
Size: 1116 432 747 268 1162
Fragments arranged by size:
1162 1116 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 864 864
Size: 3725
EcoRI G'AATT_C
Cuts at: 729 729
Size: 3725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1744 1744
Size: 3725
FspI TGC'GCA
Cuts at: 1506 1506
Size: 3725
HaeII r_GCGC'y
Cuts at: 1159 1407 2429 3408 1159
Size: 248 1022 979 1476
Fragments arranged by size:
1476 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1567 1597 1599 1629 1567
Size: 30 2 30 3663
Fragments arranged by size:
3663 30 30 2
HincII GTy'rAC
Cuts at: 764 764
Size: 3725
KasI G'GCGC_C
Cuts at: 1155 1403 1155
Size: 248 3477
KpnI G_GTAC'C
Cuts at: 745 745
Size: 3725
MluI A'CGCG_T
Cuts at: 3158 3158
Size: 3725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 484 2396 2580 484
Size: 1912 184 1629
Fragments arranged by size:
1912 1629 184
MscI TGG'CCA
Cuts at: 429 1486 429
Size: 1057 2668
MslI CAynn'nnrTG
Cuts at: 1714 1841 1714
Size: 127 3598
MspA1I CmG'CkG
Cuts at: 166 387 836 1150 1510 2523 2768 166
Size: 221 449 314 360 1013 245 1123
Fragments arranged by size:
1123 1013 449 360 314 245 221
NaeI GCC'GGC
Cuts at: 1906 1906
Size: 3725
NarI GG'CG_CC
Cuts at: 1156 1404 1156
Size: 248 3477
NdeI CA'TA_TG
Cuts at: 117 117
Size: 3725
NgoMIV G'CCGG_C
Cuts at: 1904 1904
Size: 3725
NruI TCG'CGA
Cuts at: 323 323
Size: 3725
NspI r_CATG'y
Cuts at: 778 1278 2185 778
Size: 500 907 2318
Fragments arranged by size:
2318 907 500
PciI A'CATG_T
Cuts at: 2181 2181
Size: 3725
PfoI T'CCnGG_A
Cuts at: 275 437 275
Size: 162 3563
PmeI GTTT'AAAC
Cuts at: 724 724
Size: 3725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2864 2896 2864
Size: 32 3693
PsiI TTA'TAA
Cuts at: 488 488
Size: 3725
PstI C_TGCA'G
Cuts at: 571 772 1457 571
Size: 201 685 2839
Fragments arranged by size:
2839 685 201
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3725
PvuII CAG'CTG
Cuts at: 166 387 1150 1510 166
Size: 221 763 360 2381
Fragments arranged by size:
2381 763 360 221
RsrII CG'GwC_CG
Cuts at: 1920 1920
Size: 3725
SacI G_AGCT'C
Cuts at: 739 739
Size: 3725
SalI G'TCGA_C
Cuts at: 762 762
Size: 3725
SapI GCTCTTCn'nnn_
Cuts at: 207 1748 1958 207
Size: 1541 210 1974
Fragments arranged by size:
1974 1541 210
SbfI CC_TGCA'GG
Cuts at: 772 772
Size: 3725
ScaI AGT'ACT
Cuts at: 3242 3242
Size: 3725
SfcI C'TryA_G
Cuts at: 33 567 699 768 1453 2446 2637 33
Size: 534 132 69 685 993 191 1121
Fragments arranged by size:
1121 993 685 534 191 132 69
SfoI GGC'GCC
Cuts at: 1157 1405 1157
Size: 248 3477
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
SmaI CCC'GGG
Cuts at: 747 747
Size: 3725
SmlI C'TyrA_G
Cuts at: 627 880 1795 2287 2549 627
Size: 253 915 492 262 1803
Fragments arranged by size:
1803 915 492 262 253
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3725
SphI G_CATG'C
Cuts at: 778 1278 778
Size: 500 3225
SspI AAT'ATT
Cuts at: 180 180
Size: 3725
StyI C'CwwG_G
Cuts at: 859 1213 859
Size: 354 3371
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1308 1441 1900 2109 1308
Size: 133 459 209 2924
Fragments arranged by size:
2924 459 209 133
TatI w'GTAC_w
Cuts at: 596 3240 596
Size: 2644 1081
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 3693
Tth111I GACn'n_nGTC
Cuts at: 500 1522 500
Size: 1022 2703
XbaI T'CTAG_A
Cuts at: 345 756 345
Size: 411 3314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1220 1220
Size: 3725
XmaI C'CCGG_G
Cuts at: 745 745
Size: 3725
XmnI GAAnn'nnTTC
Cuts at: 201 304 201
Size: 103 3622
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NruI NspI PciI PfoI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NheI NotI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI