Restriction map for
pF1KB9647
(Circular) (Six-base) MAPSORT of: pF1KB9647.seq Check: 1677 from: 1 to: 5051
pF1KB9647 5051 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 1239 1575 1995 2089 1239
Size: 336 420 94 4201
Fragments arranged by size:
4201 420 336 94
Acc65I G'GTAC_C
Cuts at: 2067 2067
Size: 5051
AclI AA'CG_TT
Cuts at: 4891 4891
Size: 5051
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 944 1196 1784 2048 2876 3308 4055 4323
Size: 168 252 588 264 828 432 747 268
Cuts at: 4323 776
Size: 1504
Fragments arranged by size:
1504 828 747 588 432 268 264 252
168
AflIII A'CryG_T
Cuts at: 2403 3507 4484 2403
Size: 1104 977 2970
Fragments arranged by size:
2970 1104 977
AgeI A'CCGG_T
Cuts at: 3439 3439
Size: 5051
AleI CACnn'nnGTG
Cuts at: 773 941 773
Size: 168 4883
AlwNI CAG_nnn'CTG
Cuts at: 2356 3923 2356
Size: 1567 3484
ApaLI G'TGCA_C
Cuts at: 124 3821 124
Size: 3697 1354
ApoI r'AATT_y
Cuts at: 225 329 345 494 688 830 998 1418 1586
Size: 104 16 149 194 142 168 420 168
Cuts at: 1586 1670 2018 2055 4404 4660 225
Size: 84 348 37 2349 256 616
Fragments arranged by size:
2349 616 420 348 256 194 168 168
149 142 104 84 37 16
AseI AT'TA_AT
Cuts at: 20 20
Size: 5051
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5051
AvaI C'yCGr_G
Cuts at: 2071 2071
Size: 5051
BamHI G'GATC_C
Cuts at: 2076 2076
Size: 5051
BanI G'GyrC_C
Cuts at: 2067 2481 2729 2764 2067
Size: 414 248 35 4354
Fragments arranged by size:
4354 414 248 35
BanII G_rGCy'C
Cuts at: 2065 3095 2065
Size: 1030 4021
BbeI G_GCGC'C
Cuts at: 2485 2733 2485
Size: 248 4803
BbsI GAAGACnn'nnnn_
Cuts at: 965 1973 965
Size: 1008 4043
BciVI GTATCCnnnnn_n'
Cuts at: 2949 3710 5051 2949
Size: 761 1341 2949
Fragments arranged by size:
2949 1341 761
BclI T'GATC_A
Cuts at: 2571 2571
Size: 5051
BglII A'GATC_T
Cuts at: 2566 4603 2566
Size: 2037 3014
BlpI GC'TnA_GC
Cuts at: 2163 2163
Size: 5051
Bme1580I G_kGCm'C
Cuts at: 128 1664 2676 2769 3825 128
Size: 1536 1012 93 1056 1354
Fragments arranged by size:
1536 1354 1056 1012 93
BmrI ACTGGGnnnn_n'
Cuts at: 2263 2344 2679 4823 2263
Size: 81 335 2144 2491
Fragments arranged by size:
2491 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3418 3418
Size: 5051
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 860 1028 1280 1364 1616 1868 1952 443
Size: 417 168 252 84 252 252 84 3542
Fragments arranged by size:
3542 417 252 252 252 168 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2227 3106 3598 3896 2227
Size: 879 492 298 3382
Fragments arranged by size:
3382 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4755 4755
Size: 5051
BsaAI yAC'GTr
Cuts at: 2404 2404
Size: 5051
BsaBI GATnn'nnATC
Cuts at: 6 2565 4602 6
Size: 2559 2037 455
Fragments arranged by size:
2559 2037 455
BsaHI Gr'CG_yC
Cuts at: 2482 2730 3432 4928 2482
Size: 248 702 1496 2605
Fragments arranged by size:
2605 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2240 2761 3439 3713 3860 4271 2240
Size: 521 678 274 147 411 3020
Fragments arranged by size:
3020 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1657 2476 3234 3811 4307 1657
Size: 819 758 577 496 2401
Fragments arranged by size:
2401 819 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2593 2639 3847 104
Size: 2489 46 1208 1308
Fragments arranged by size:
2489 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 128 2065 2843 3033 3825 128
Size: 1937 778 190 792 1354
Fragments arranged by size:
1937 1354 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 128 1664 2065 2676 2769 2843 3033 3095 3825
Size: 1536 401 611 93 74 190 62 730
Cuts at: 3825 128
Size: 1354
Fragments arranged by size:
1536 1354 730 611 401 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4271 4271
Size: 5051
BspHI T'CATG_A
Cuts at: 4227 4227
Size: 5051
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2101 2315 2617 2998 2101
Size: 214 302 381 4154
Fragments arranged by size:
4154 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3343 3397 4881 5046 50
Size: 3293 54 1484 165 55
Fragments arranged by size:
3293 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2963 2963
Size: 5051
BsrFI r'CCGG_y
Cuts at: 3049 3230 3439 3049
Size: 181 209 4661
Fragments arranged by size:
4661 209 181
BsrGI T'GTAC_A
Cuts at: 1680 2037 1680
Size: 357 4694
BssSI C'ACGA_G
Cuts at: 3322 3680 3322
Size: 358 4693
BstAPI GCAn_nnn'nTGC
Cuts at: 2428 2428
Size: 5051
BstBI TT'CG_AA
Cuts at: 3412 3412
Size: 5051
BstYI r'GATC_y
Cuts at: 1 2076 2543 2566 2901 3147 4159 4245 4257
Size: 2075 467 23 335 246 1012 86 12
Cuts at: 4257 4603 1
Size: 346 449
Fragments arranged by size:
2075 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1240 1576 1240
Size: 336 4715
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3001 3182 3345 3001
Size: 181 163 4707
Fragments arranged by size:
4707 181 163
BtsI GCAGTG_nn'
Cuts at: 2294 2294
Size: 5051
DraI TTT'AAA
Cuts at: 224 1134 1302 2050 224
Size: 910 168 748 3225
Fragments arranged by size:
3225 910 748 168
DrdI GACnn_nn'nnGTC
Cuts at: 2757 3615 2757
Size: 858 4193
EaeI y'GGCC_r
Cuts at: 2636 2810 3201 3228 4573 2636
Size: 174 391 27 1345 3114
Fragments arranged by size:
3114 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2636 2636
Size: 5051
EarI CTCTTCn'nnn_
Cuts at: 752 920 1088 1172 1760 2024 3074 3284 752
Size: 168 168 84 588 264 1050 210 2519
Fragments arranged by size:
2519 1050 588 264 210 168 168 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3259 3569 3715 4861 3259
Size: 310 146 1146 3449
Fragments arranged by size:
3449 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2063 2063
Size: 5051
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 776 860 944 1028 1196 1280 1364 1616
Size: 333 84 84 84 168 84 84 252
Cuts at: 1616 1784 1868 1952 2048 2876 3308 4055 4323
Size: 168 84 84 96 828 432 747 268
Cuts at: 4323 443
Size: 1171
Fragments arranged by size:
1171 828 747 432 333 268 252 168
168 96 84 84 84 84 84 84
84
EcoO109I rG'GnC_Cy
Cuts at: 2190 2190
Size: 5051
EcoRI G'AATT_C
Cuts at: 329 345 494 830 998 1418 2018 2055 329
Size: 16 149 336 168 420 600 37 3325
Fragments arranged by size:
3325 600 420 336 168 149 37 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3070 3070
Size: 5051
FspI TGC'GCA
Cuts at: 2832 2832
Size: 5051
HaeII r_GCGC'y
Cuts at: 2485 2733 3755 4734 2485
Size: 248 1022 979 2802
Fragments arranged by size:
2802 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1550 1582 2893 2923 2925 2955 1550
Size: 32 1311 30 2 30 3646
Fragments arranged by size:
3646 1311 32 30 30 2
HincII GTy'rAC
Cuts at: 2090 2090
Size: 5051
HindIII A'AGCT_T
Cuts at: 791 959 1127 1211 1295 1379 1547 1631 1883
Size: 168 168 84 84 84 168 84 252
Cuts at: 1883 1967 791
Size: 84 3875
Fragments arranged by size:
3875 252 168 168 168 84 84 84
84 84
KasI G'GCGC_C
Cuts at: 2481 2729 2481
Size: 248 4803
KpnI G_GTAC'C
Cuts at: 2071 2071
Size: 5051
MluI A'CGCG_T
Cuts at: 4484 4484
Size: 5051
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3722 3906 3722
Size: 184 4867
MscI TGG'CCA
Cuts at: 2812 2812
Size: 5051
MslI CAynn'nnrTG
Cuts at: 773 941 3040 3167 773
Size: 168 2099 127 2657
Fragments arranged by size:
2657 2099 168 127
MspA1I CmG'CkG
Cuts at: 2162 2476 2836 3849 4094 2162
Size: 314 360 1013 245 3119
Fragments arranged by size:
3119 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3232 3232
Size: 5051
NarI GG'CG_CC
Cuts at: 2482 2730 2482
Size: 248 4803
NgoMIV G'CCGG_C
Cuts at: 3230 3230
Size: 5051
NspI r_CATG'y
Cuts at: 303 1961 2104 2604 3511 303
Size: 1658 143 500 907 1843
Fragments arranged by size:
1843 1658 907 500 143
PciI A'CATG_T
Cuts at: 3507 3507
Size: 5051
PmeI GTTT'AAAC
Cuts at: 2050 2050
Size: 5051
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 4190
Size: 32 5019
PsiI TTA'TAA
Cuts at: 140 797 965 140
Size: 657 168 4226
Fragments arranged by size:
4226 657 168
PstI C_TGCA'G
Cuts at: 2098 2783 2098
Size: 685 4366
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5051
PvuII CAG'CTG
Cuts at: 2476 2836 2476
Size: 360 4691
RsrII CG'GwC_CG
Cuts at: 3246 3246
Size: 5051
SacI G_AGCT'C
Cuts at: 2065 2065
Size: 5051
SalI G'TCGA_C
Cuts at: 2088 2088
Size: 5051
SapI GCTCTTCn'nnn_
Cuts at: 3074 3284 3074
Size: 210 4841
SbfI CC_TGCA'GG
Cuts at: 2098 2098
Size: 5051
ScaI AGT'ACT
Cuts at: 4568 4568
Size: 5051
SfcI C'TryA_G
Cuts at: 33 2094 2779 3772 3963 33
Size: 2061 685 993 191 1121
Fragments arranged by size:
2061 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2483 2731 2483
Size: 248 4803
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5051
SmaI CCC'GGG
Cuts at: 2073 2073
Size: 5051
SmlI C'TyrA_G
Cuts at: 2206 3121 3613 3875 2206
Size: 915 492 262 3382
Fragments arranged by size:
3382 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5051
SphI G_CATG'C
Cuts at: 303 2104 2604 303
Size: 1801 500 2750
Fragments arranged by size:
2750 1801 500
SspI AAT'ATT
Cuts at: 182 784 182
Size: 602 4449
StyI C'CwwG_G
Cuts at: 2185 2539 2185
Size: 354 4697
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1379 2634 2767 3226 3435 1379
Size: 1255 133 459 209 2995
Fragments arranged by size:
2995 1255 459 209 133
TatI w'GTAC_w
Cuts at: 1680 2037 4566 1680
Size: 357 2529 2165
Fragments arranged by size:
2529 2165 357
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4568 4600 4568
Size: 32 5019
Tth111I GACn'n_nGTC
Cuts at: 2848 2848
Size: 5051
XbaI T'CTAG_A
Cuts at: 2082 2082
Size: 5051
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2546 2546
Size: 5051
XmaI C'CCGG_G
Cuts at: 2071 2071
Size: 5051
XmnI GAAnn'nnTTC
Cuts at: 265 265
Size: 5051
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BssHII BstEII BstXI Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI