Restriction map for
pF1KB9649
(Circular) (Six-base) MAPSORT of: pF1KB9649.seq Check: 5705 from: 1 to: 4436
pF1KB9649 4436 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1474 1474
Size: 4436
Acc65I G'GTAC_C
Cuts at: 1452 1452
Size: 4436
AclI AA'CG_TT
Cuts at: 4276 4276
Size: 4436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 2261 2693 3440 3708 397
Size: 1864 432 747 268 1125
Fragments arranged by size:
1864 1125 747 432 268
AfeI AGC'GCT
Cuts at: 364 364
Size: 4436
AflIII A'CryG_T
Cuts at: 269 1788 2892 3869 269
Size: 1519 1104 977 836
Fragments arranged by size:
1519 1104 977 836
AgeI A'CCGG_T
Cuts at: 2824 2824
Size: 4436
AlwNI CAG_nnn'CTG
Cuts at: 873 906 1248 1741 3308 873
Size: 33 342 493 1567 2001
Fragments arranged by size:
2001 1567 493 342 33
ApaLI G'TGCA_C
Cuts at: 3206 3206
Size: 4436
ApoI r'AATT_y
Cuts at: 181 1209 1440 3789 4045 181
Size: 1028 231 2349 256 572
Fragments arranged by size:
2349 1028 572 256 231
AseI AT'TA_AT
Cuts at: 20 20
Size: 4436
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
AvaI C'yCGr_G
Cuts at: 152 729 970 1014 1456 152
Size: 577 241 44 442 3132
Fragments arranged by size:
3132 577 442 241 44
BamHI G'GATC_C
Cuts at: 1461 1461
Size: 4436
BanI G'GyrC_C
Cuts at: 574 637 1018 1452 1866 2114 2149 574
Size: 63 381 434 414 248 35 2861
Fragments arranged by size:
2861 434 414 381 248 63 35
BanII G_rGCy'C
Cuts at: 520 595 1333 1450 2480 520
Size: 75 738 117 1030 2476
Fragments arranged by size:
2476 1030 738 117 75
BbeI G_GCGC'C
Cuts at: 641 1022 1870 2118 641
Size: 381 848 248 2959
Fragments arranged by size:
2959 848 381 248
BciVI GTATCCnnnnn_n'
Cuts at: 2334 3095 4436 2334
Size: 761 1341 2334
Fragments arranged by size:
2334 1341 761
BclI T'GATC_A
Cuts at: 1956 1956
Size: 4436
BglI GCCn_nnn'nGGC
Cuts at: 628 885 628
Size: 257 4179
BglII A'GATC_T
Cuts at: 341 1951 3988 341
Size: 1610 2037 789
Fragments arranged by size:
2037 1610 789
BlpI GC'TnA_GC
Cuts at: 949 1369 1548 949
Size: 420 179 3837
Fragments arranged by size:
3837 420 179
Bme1580I G_kGCm'C
Cuts at: 935 2061 2154 3210 935
Size: 1126 93 1056 2161
Fragments arranged by size:
2161 1126 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 660 1648 1729 2064 4208 660
Size: 988 81 335 2144 888
Fragments arranged by size:
2144 988 888 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1000 2803 1000
Size: 1803 2633
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 579 1417 579
Size: 838 3598
Bpu10I CC'TnA_GC
Cuts at: 1201 1201
Size: 4436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 601 1612 2491 2983 3281 412
Size: 189 1011 879 492 298 1567
Fragments arranged by size:
1567 1011 879 492 298 189
BsaI GGTCTCn'nnnn_
Cuts at: 4140 4140
Size: 4436
BsaAI yAC'GTr
Cuts at: 702 1789 702
Size: 1087 3349
BsaBI GATnn'nnATC
Cuts at: 6 1950 3987 6
Size: 1944 2037 455
Fragments arranged by size:
2037 1944 455
BsaHI Gr'CG_yC
Cuts at: 241 638 1019 1178 1867 2115 2817 4313 241
Size: 397 381 159 689 248 702 1496 364
Fragments arranged by size:
1496 702 689 397 381 364 248 159
BsaWI w'CCGG_w
Cuts at: 321 1625 2146 2824 3098 3245 3656 321
Size: 1304 521 678 274 147 411 1101
Fragments arranged by size:
1304 1101 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 187 856 1122 187
Size: 669 266 3501
Fragments arranged by size:
3501 669 266
BseYI C'CCAG_C
Cuts at: 354 502 705 1248 1861 2619 3196 3692 354
Size: 148 203 543 613 758 577 496 1098
Fragments arranged by size:
1098 758 613 577 543 496 203 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 1341 133
Size: 1208 3228
BsiEI CG_ry'CG
Cuts at: 104 262 536 632 1268 1978 2024 3232 104
Size: 158 274 96 636 710 46 1208 1308
Fragments arranged by size:
1308 1208 710 636 274 158 96 46
BsiHKAI G_wGCw'C
Cuts at: 1450 2228 2418 3210 1450
Size: 778 190 792 2676
Fragments arranged by size:
2676 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 903 903
Size: 4436
Bsp1286I G_dGCh'C
Cuts at: 520 595 935 1333 1450 2061 2154 2228 2418
Size: 75 340 398 117 611 93 74 190
Cuts at: 2418 2480 3210 520
Size: 62 730 1746
Fragments arranged by size:
1746 730 611 398 340 190 117 93
75 74 62
BspEI T'CCGG_A
Cuts at: 321 3656 321
Size: 3335 1101
BspHI T'CATG_A
Cuts at: 3612 3612
Size: 4436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 155 1486 1700 2002 2383 155
Size: 1331 214 302 381 2208
Fragments arranged by size:
2208 1331 381 302 214
BsrBI CCG'CTC
Cuts at: 50 417 725 1083 2728 2782 4266 4431 50
Size: 367 308 358 1645 54 1484 165 55
Fragments arranged by size:
1645 1484 367 358 308 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2348 2348
Size: 4436
BsrFI r'CCGG_y
Cuts at: 461 535 1003 2434 2615 2824 461
Size: 74 468 1431 181 209 2073
Fragments arranged by size:
2073 1431 468 209 181 74
BssHII G'CGCG_C
Cuts at: 1223 1223
Size: 4436
BssSI C'ACGA_G
Cuts at: 2707 3065 2707
Size: 358 4078
BstAPI GCAn_nnn'nTGC
Cuts at: 605 873 1813 605
Size: 268 940 3228
Fragments arranged by size:
3228 940 268
BstBI TT'CG_AA
Cuts at: 2797 2797
Size: 4436
BstXI CCAn_nnnn'nTGG
Cuts at: 354 354
Size: 4436
BstYI r'GATC_y
Cuts at: 1 341 392 1461 1928 1951 2286 2532 3544
Size: 340 51 1069 467 23 335 246 1012
Cuts at: 3544 3630 3642 3988 1
Size: 86 12 346 449
Fragments arranged by size:
1069 1012 467 449 346 340 335 246
86 51 23 12
BtgI C'CryG_G
Cuts at: 107 626 632 107
Size: 519 6 3911
Fragments arranged by size:
3911 519 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2386 2567 2730 2386
Size: 181 163 4092
Fragments arranged by size:
4092 181 163
BtsI GCAGTG_nn'
Cuts at: 1679 1679
Size: 4436
DraI TTT'AAA
Cuts at: 1435 1435
Size: 4436
DrdI GACnn_nn'nnGTC
Cuts at: 2142 3000 2142
Size: 858 3578
EaeI y'GGCC_r
Cuts at: 259 437 494 533 629 1265 2021 2195 2586
Size: 178 57 39 96 636 756 174 391
Cuts at: 2586 2613 3958 259
Size: 27 1345 737
Fragments arranged by size:
1345 756 737 636 391 178 174 96
57 39 27
EagI C'GGCC_G
Cuts at: 259 533 629 1265 2021 259
Size: 274 96 636 756 2674
Fragments arranged by size:
2674 756 636 274 96
EarI CTCTTCn'nnn_
Cuts at: 520 2459 2669 520
Size: 1939 210 2287
Fragments arranged by size:
2287 1939 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 136 427 575 976 2644 2954 3100 4246 136
Size: 291 148 401 1668 310 146 1146 326
Fragments arranged by size:
1668 1146 401 326 310 291 148 146
EcoICRI GAG'CTC
Cuts at: 1448 1448
Size: 4436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 579 1417 2261 2693 3440 3708 397
Size: 182 838 844 432 747 268 1125
Fragments arranged by size:
1125 844 838 747 432 268 182
EcoO109I rG'GnC_Cy
Cuts at: 621 1575 621
Size: 954 3482
EcoRI G'AATT_C
Cuts at: 181 1440 181
Size: 1259 3177
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 4436
FspI TGC'GCA
Cuts at: 2217 2217
Size: 4436
HaeII r_GCGC'y
Cuts at: 163 366 509 641 1022 1235 1870 2118 3140
Size: 203 143 132 381 213 635 248 1022
Cuts at: 3140 4119 163
Size: 979 480
Fragments arranged by size:
1022 979 635 480 381 248 213 203
143 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2278 2308 2310 2340 2278
Size: 30 2 30 4374
Fragments arranged by size:
4374 30 30 2
HincII GTy'rAC
Cuts at: 1475 1475
Size: 4436
KasI G'GCGC_C
Cuts at: 637 1018 1866 2114 637
Size: 381 848 248 2959
Fragments arranged by size:
2959 848 381 248
KpnI G_GTAC'C
Cuts at: 1456 1456
Size: 4436
MluI A'CGCG_T
Cuts at: 269 3869 269
Size: 3600 836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1436 3107 3291 1436
Size: 1671 184 2581
Fragments arranged by size:
2581 1671 184
MscI TGG'CCA
Cuts at: 2197 2197
Size: 4436
MslI CAynn'nnrTG
Cuts at: 2425 2552 2425
Size: 127 4309
MspA1I CmG'CkG
Cuts at: 628 634 816 903 1148 1160 1169 1248 1547
Size: 6 182 87 245 12 9 79 299
Cuts at: 1547 1861 2221 3234 3479 628
Size: 314 360 1013 245 1585
Fragments arranged by size:
1585 1013 360 314 299 245 245 182
87 79 12 9 6
NaeI GCC'GGC
Cuts at: 537 1005 2617 537
Size: 468 1612 2356
Fragments arranged by size:
2356 1612 468
NarI GG'CG_CC
Cuts at: 638 1019 1867 2115 638
Size: 381 848 248 2959
Fragments arranged by size:
2959 848 381 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4436
NdeI CA'TA_TG
Cuts at: 1428 1428
Size: 4436
NgoMIV G'CCGG_C
Cuts at: 535 1003 2615 535
Size: 468 1612 2356
Fragments arranged by size:
2356 1612 468
NotI GC'GGCC_GC
Cuts at: 629 629
Size: 4436
NspI r_CATG'y
Cuts at: 330 1489 1989 2896 330
Size: 1159 500 907 1870
Fragments arranged by size:
1870 1159 907 500
PciI A'CATG_T
Cuts at: 2892 2892
Size: 4436
PfoI T'CCnGG_A
Cuts at: 404 404
Size: 4436
PmeI GTTT'AAAC
Cuts at: 1435 1435
Size: 4436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 3575
Size: 32 4404
PstI C_TGCA'G
Cuts at: 815 821 878 1483 2168 815
Size: 6 57 605 685 3083
Fragments arranged by size:
3083 685 605 57 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4436
PvuII CAG'CTG
Cuts at: 816 903 1248 1861 2221 816
Size: 87 345 613 360 3031
Fragments arranged by size:
3031 613 360 345 87
RsrII CG'GwC_CG
Cuts at: 2631 2631
Size: 4436
SacI G_AGCT'C
Cuts at: 1450 1450
Size: 4436
SacII CC_GC'GG
Cuts at: 629 635 629
Size: 6 4430
SalI G'TCGA_C
Cuts at: 1473 1473
Size: 4436
SapI GCTCTTCn'nnn_
Cuts at: 520 2459 2669 520
Size: 1939 210 2287
Fragments arranged by size:
2287 1939 210
SbfI CC_TGCA'GG
Cuts at: 1483 1483
Size: 4436
ScaI AGT'ACT
Cuts at: 3953 3953
Size: 4436
SexAI A'CCwGG_T
Cuts at: 1415 1415
Size: 4436
SfcI C'TryA_G
Cuts at: 33 811 817 874 1044 1479 2164 3157 3348
Size: 778 6 57 170 435 685 993 191
Cuts at: 3348 33
Size: 1121
Fragments arranged by size:
1121 993 778 685 435 191 170 57
6
SfiI GGCCn_nnn'nGGCC
Cuts at: 628 628
Size: 4436
SfoI GGC'GCC
Cuts at: 639 1020 1868 2116 639
Size: 381 848 248 2959
Fragments arranged by size:
2959 848 381 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
SmaI CCC'GGG
Cuts at: 154 1458 154
Size: 1304 3132
SmlI C'TyrA_G
Cuts at: 427 616 1591 2506 2998 3260 427
Size: 189 975 915 492 262 1603
Fragments arranged by size:
1603 975 915 492 262 189
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4436
SphI G_CATG'C
Cuts at: 1489 1989 1489
Size: 500 3936
StyI C'CwwG_G
Cuts at: 107 587 641 1570 1924 107
Size: 480 54 929 354 2619
Fragments arranged by size:
2619 929 480 354 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2019 2152 2611 2820 2019
Size: 133 459 209 3635
Fragments arranged by size:
3635 459 209 133
TatI w'GTAC_w
Cuts at: 3951 3951
Size: 4436
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3953 3985 3953
Size: 32 4404
Tth111I GACn'n_nGTC
Cuts at: 240 2233 240
Size: 1993 2443
XbaI T'CTAG_A
Cuts at: 1467 1467
Size: 4436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1931 1931
Size: 4436
XmaI C'CCGG_G
Cuts at: 152 1456 152
Size: 1304 3132
XmnI GAAnn'nnTTC
Cuts at: 271 271
Size: 4436
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NotI NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BsaXI BsiWI BsmI BsrGI BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI