Restriction map for
pF1KB9651
(Circular) (Six-base) MAPSORT of: pF1KB9651.seq Check: 910 from: 1 to: 3833
pF1KB9651 3833 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 871 871
Size: 3833
Acc65I G'GTAC_C
Cuts at: 849 849
Size: 3833
AclI AA'CG_TT
Cuts at: 3673 3673
Size: 3833
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 1658 2090 2837 3105 104
Size: 1554 432 747 268 832
Fragments arranged by size:
1554 832 747 432 268
AflIII A'CryG_T
Cuts at: 605 1185 2289 3266 605
Size: 580 1104 977 1172
Fragments arranged by size:
1172 1104 977 580
AgeI A'CCGG_T
Cuts at: 2221 2221
Size: 3833
AlwNI CAG_nnn'CTG
Cuts at: 1138 2705 1138
Size: 1567 2266
ApaLI G'TGCA_C
Cuts at: 2603 2603
Size: 3833
ApoI r'AATT_y
Cuts at: 837 3186 3442 837
Size: 2349 256 1228
Fragments arranged by size:
2349 1228 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3833
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
AvaI C'yCGr_G
Cuts at: 164 472 482 543 853 164
Size: 308 10 61 310 3144
Fragments arranged by size:
3144 310 308 61 10
AvrII C'CTAG_G
Cuts at: 231 231
Size: 3833
BamHI G'GATC_C
Cuts at: 858 858
Size: 3833
BanI G'GyrC_C
Cuts at: 235 507 631 705 849 1263 1511 1546 235
Size: 272 124 74 144 414 248 35 2522
Fragments arranged by size:
2522 414 272 248 144 124 74 35
BanII G_rGCy'C
Cuts at: 847 1877 847
Size: 1030 2803
BbeI G_GCGC'C
Cuts at: 511 635 1267 1515 511
Size: 124 632 248 2829
Fragments arranged by size:
2829 632 248 124
BbsI GAAGACnn'nnnn_
Cuts at: 378 378
Size: 3833
BbvCI CC'TCA_GC
Cuts at: 646 646
Size: 3833
BciVI GTATCCnnnnn_n'
Cuts at: 1731 2492 3833 1731
Size: 761 1341 1731
Fragments arranged by size:
1731 1341 761
BclI T'GATC_A
Cuts at: 1353 1353
Size: 3833
BglII A'GATC_T
Cuts at: 1348 3385 1348
Size: 2037 1796
BlpI GC'TnA_GC
Cuts at: 945 945
Size: 3833
Bme1580I G_kGCm'C
Cuts at: 575 710 1458 1551 2607 575
Size: 135 748 93 1056 1801
Fragments arranged by size:
1801 1056 748 135 93
BmrI ACTGGGnnnn_n'
Cuts at: 1045 1126 1461 3605 1045
Size: 81 335 2144 1273
Fragments arranged by size:
2144 1273 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 3833
Bpu10I CC'TnA_GC
Cuts at: 463 646 463
Size: 183 3650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 406 1009 1888 2380 2678 352
Size: 54 603 879 492 298 1507
Fragments arranged by size:
1507 879 603 492 298 54
BsaI GGTCTCn'nnnn_
Cuts at: 3537 3537
Size: 3833
BsaAI yAC'GTr
Cuts at: 702 1186 702
Size: 484 3349
BsaBI GATnn'nnATC
Cuts at: 6 1347 3384 6
Size: 1341 2037 455
Fragments arranged by size:
2037 1341 455
BsaHI Gr'CG_yC
Cuts at: 508 632 1264 1512 2214 3710 508
Size: 124 632 248 702 1496 631
Fragments arranged by size:
1496 702 632 631 248 124
BsaWI w'CCGG_w
Cuts at: 1022 1543 2221 2495 2642 3053 1022
Size: 521 678 274 147 411 1802
Fragments arranged by size:
1802 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 305 655 1258 2016 2593 3089 305
Size: 350 603 758 577 496 1049
Fragments arranged by size:
1049 758 603 577 496 350
BsiEI CG_ry'CG
Cuts at: 104 1375 1421 2629 104
Size: 1271 46 1208 1308
Fragments arranged by size:
1308 1271 1208 46
BsiHKAI G_wGCw'C
Cuts at: 323 847 1625 1815 2607 323
Size: 524 778 190 792 1549
Fragments arranged by size:
1549 792 778 524 190
Bsp1286I G_dGCh'C
Cuts at: 323 575 710 847 1458 1551 1625 1815 1877
Size: 252 135 137 611 93 74 190 62
Cuts at: 1877 2607 323
Size: 730 1549
Fragments arranged by size:
1549 730 611 252 190 137 135 93
74 62
BspEI T'CCGG_A
Cuts at: 3053 3053
Size: 3833
BspHI T'CATG_A
Cuts at: 3009 3009
Size: 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 371 883 1097 1399 1780 371
Size: 512 214 302 381 2424
Fragments arranged by size:
2424 512 381 302 214
BsrBI CCG'CTC
Cuts at: 50 420 2125 2179 3663 3828 50
Size: 370 1705 54 1484 165 55
Fragments arranged by size:
1705 1484 370 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1745 1745
Size: 3833
BsrFI r'CCGG_y
Cuts at: 498 589 1831 2012 2221 498
Size: 91 1242 181 209 2110
Fragments arranged by size:
2110 1242 209 181 91
BssHII G'CGCG_C
Cuts at: 467 467
Size: 3833
BssSI C'ACGA_G
Cuts at: 2104 2462 2104
Size: 358 3475
BstAPI GCAn_nnn'nTGC
Cuts at: 1210 1210
Size: 3833
BstBI TT'CG_AA
Cuts at: 2194 2194
Size: 3833
BstYI r'GATC_y
Cuts at: 1 858 1325 1348 1683 1929 2941 3027 3039
Size: 857 467 23 335 246 1012 86 12
Cuts at: 3039 3385 1
Size: 346 449
Fragments arranged by size:
1012 857 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 440 824 440
Size: 384 3449
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1783 1964 2127 1783
Size: 181 163 3489
Fragments arranged by size:
3489 181 163
BtsI GCAGTG_nn'
Cuts at: 1076 1076
Size: 3833
ClaI AT'CG_AT
Cuts at: 281 281
Size: 3833
DraI TTT'AAA
Cuts at: 832 832
Size: 3833
DrdI GACnn_nn'nnGTC
Cuts at: 382 1539 2397 382
Size: 1157 858 1818
Fragments arranged by size:
1818 1157 858
EaeI y'GGCC_r
Cuts at: 271 821 1418 1592 1983 2010 3355 271
Size: 550 597 174 391 27 1345 749
Fragments arranged by size:
1345 749 597 550 391 174 27
EagI C'GGCC_G
Cuts at: 1418 1418
Size: 3833
EarI CTCTTCn'nnn_
Cuts at: 772 1856 2066 772
Size: 1084 210 2539
Fragments arranged by size:
2539 1084 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2041 2351 2497 3643 2041
Size: 310 146 1146 2231
Fragments arranged by size:
2231 1146 310 146
EcoICRI GAG'CTC
Cuts at: 845 845
Size: 3833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 1658 2090 2837 3105 104
Size: 1554 432 747 268 832
Fragments arranged by size:
1554 832 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 446 972 446
Size: 526 3307
EcoRI G'AATT_C
Cuts at: 837 837
Size: 3833
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1852 1852
Size: 3833
FspI TGC'GCA
Cuts at: 1614 1614
Size: 3833
HaeII r_GCGC'y
Cuts at: 511 551 596 635 1267 1515 2537 3516 511
Size: 40 45 39 632 248 1022 979 828
Fragments arranged by size:
1022 979 828 632 248 45 40 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1675 1705 1707 1737 1675
Size: 30 2 30 3771
Fragments arranged by size:
3771 30 30 2
HincII GTy'rAC
Cuts at: 872 872
Size: 3833
HindIII A'AGCT_T
Cuts at: 247 247
Size: 3833
KasI G'GCGC_C
Cuts at: 507 631 1263 1511 507
Size: 124 632 248 2829
Fragments arranged by size:
2829 632 248 124
KpnI G_GTAC'C
Cuts at: 853 853
Size: 3833
MluI A'CGCG_T
Cuts at: 605 3266 605
Size: 2661 1172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 476 2504 2688 476
Size: 2028 184 1621
Fragments arranged by size:
2028 1621 184
MscI TGG'CCA
Cuts at: 1594 1594
Size: 3833
MslI CAynn'nnrTG
Cuts at: 1822 1949 1822
Size: 127 3706
MspA1I CmG'CkG
Cuts at: 174 650 944 1258 1618 2631 2876 174
Size: 476 294 314 360 1013 245 1131
Fragments arranged by size:
1131 1013 476 360 314 294 245
NaeI GCC'GGC
Cuts at: 500 2014 500
Size: 1514 2319
NarI GG'CG_CC
Cuts at: 508 632 1264 1512 508
Size: 124 632 248 2829
Fragments arranged by size:
2829 632 248 124
NcoI C'CATG_G
Cuts at: 824 824
Size: 3833
NgoMIV G'CCGG_C
Cuts at: 498 2012 498
Size: 1514 2319
NspI r_CATG'y
Cuts at: 886 1386 2293 886
Size: 500 907 2426
Fragments arranged by size:
2426 907 500
PasI CC'CwG_GG
Cuts at: 615 615
Size: 3833
PciI A'CATG_T
Cuts at: 2289 2289
Size: 3833
PflMI CCAn_nnn'nTGG
Cuts at: 561 582 561
Size: 21 3812
PmeI GTTT'AAAC
Cuts at: 832 832
Size: 3833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2972 3004 2972
Size: 32 3801
PspXI vC'TCGA_Gb
Cuts at: 543 543
Size: 3833
PstI C_TGCA'G
Cuts at: 746 880 1565 746
Size: 134 685 3014
Fragments arranged by size:
3014 685 134
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3833
PvuII CAG'CTG
Cuts at: 650 1258 1618 650
Size: 608 360 2865
Fragments arranged by size:
2865 608 360
RsrII CG'GwC_CG
Cuts at: 2028 2028
Size: 3833
SacI G_AGCT'C
Cuts at: 847 847
Size: 3833
SalI G'TCGA_C
Cuts at: 870 870
Size: 3833
SapI GCTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3623
SbfI CC_TGCA'GG
Cuts at: 880 880
Size: 3833
ScaI AGT'ACT
Cuts at: 3350 3350
Size: 3833
SfcI C'TryA_G
Cuts at: 33 742 876 1561 2554 2745 33
Size: 709 134 685 993 191 1121
Fragments arranged by size:
1121 993 709 685 191 134
SfoI GGC'GCC
Cuts at: 509 633 1265 1513 509
Size: 124 632 248 2829
Fragments arranged by size:
2829 632 248 124
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
SmaI CCC'GGG
Cuts at: 166 484 855 166
Size: 318 371 3144
Fragments arranged by size:
3144 371 318
SmlI C'TyrA_G
Cuts at: 367 421 543 988 1903 2395 2657 367
Size: 54 122 445 915 492 262 1543
Fragments arranged by size:
1543 915 492 445 262 122 54
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3833
SphI G_CATG'C
Cuts at: 886 1386 886
Size: 500 3333
SrfI GCCC'GGGC
Cuts at: 484 484
Size: 3833
StyI C'CwwG_G
Cuts at: 231 581 824 967 1321 231
Size: 350 243 143 354 2743
Fragments arranged by size:
2743 354 350 243 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1416 1549 2008 2217 1416
Size: 133 459 209 3032
Fragments arranged by size:
3032 459 209 133
TatI w'GTAC_w
Cuts at: 3348 3348
Size: 3833
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3350 3382 3350
Size: 32 3801
Tth111I GACn'n_nGTC
Cuts at: 1630 1630
Size: 3833
XbaI T'CTAG_A
Cuts at: 864 864
Size: 3833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 558 1328 558
Size: 770 3063
XhoI C'TCGA_G
Cuts at: 543 543
Size: 3833
XmaI C'CCGG_G
Cuts at: 164 482 853 164
Size: 318 371 3144
Fragments arranged by size:
3144 371 318
XmnI GAAnn'nnTTC
Cuts at: 148 148
Size: 3833
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SspI
StuI SwaI ZraI