Restriction map for
pF1KB9659
(Circular) (Six-base) MAPSORT of: pF1KB9659.seq Check: 2026 from: 1 to: 4577
pF1KB9659 4577 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 1615 1615
Size: 4577
Acc65I G'GTAC_C
Cuts at: 1593 1593
Size: 4577
AclI AA'CG_TT
Cuts at: 4417 4417
Size: 4577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 2402 2834 3581 3849 254
Size: 2148 432 747 268 982
Fragments arranged by size:
2148 982 747 432 268
AflIII A'CryG_T
Cuts at: 1929 3033 4010 1929
Size: 1104 977 2496
Fragments arranged by size:
2496 1104 977
AgeI A'CCGG_T
Cuts at: 865 2965 865
Size: 2100 2477
AleI CACnn'nnGTG
Cuts at: 614 698 1034 1370 614
Size: 84 336 336 3821
Fragments arranged by size:
3821 336 336 84
AlwNI CAG_nnn'CTG
Cuts at: 1882 3449 1882
Size: 1567 3010
ApaLI G'TGCA_C
Cuts at: 3347 3347
Size: 4577
ApoI r'AATT_y
Cuts at: 269 353 437 521 689 857 941 1025 1193
Size: 84 84 84 168 168 84 84 168
Cuts at: 1193 1445 1581 3930 4186 269
Size: 252 136 2349 256 660
Fragments arranged by size:
2349 660 256 252 168 168 168 136
84 84 84 84 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 4577
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
AvaI C'yCGr_G
Cuts at: 1597 1597
Size: 4577
BamHI G'GATC_C
Cuts at: 1602 1602
Size: 4577
BanI G'GyrC_C
Cuts at: 1352 1593 2007 2255 2290 1352
Size: 241 414 248 35 3639
Fragments arranged by size:
3639 414 248 241 35
BanII G_rGCy'C
Cuts at: 1591 2621 1591
Size: 1030 3547
BbeI G_GCGC'C
Cuts at: 2011 2259 2011
Size: 248 4329
BbsI GAAGACnn'nnnn_
Cuts at: 242 1159 242
Size: 917 3660
BciVI GTATCCnnnnn_n'
Cuts at: 265 2475 3236 4577 265
Size: 2210 761 1341 265
Fragments arranged by size:
2210 1341 761 265
BclI T'GATC_A
Cuts at: 2097 2097
Size: 4577
BfrBI ATG'CAT
Cuts at: 1113 1113
Size: 4577
BglII A'GATC_T
Cuts at: 1005 2092 4129 1005
Size: 1087 2037 1453
Fragments arranged by size:
2037 1453 1087
BlpI GC'TnA_GC
Cuts at: 1689 1689
Size: 4577
Bme1580I G_kGCm'C
Cuts at: 2202 2295 3351 2202
Size: 93 1056 3428
Fragments arranged by size:
3428 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1789 1870 2205 4349 1789
Size: 81 335 2144 2017
Fragments arranged by size:
2144 2017 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2944 2944
Size: 4577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1139 1139
Size: 4577
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1407 1753 2632 3124 3422 1407
Size: 346 879 492 298 2562
Fragments arranged by size:
2562 879 492 346 298
BsaI GGTCTCn'nnnn_
Cuts at: 4281 4281
Size: 4577
BsaAI yAC'GTr
Cuts at: 1930 1930
Size: 4577
BsaBI GATnn'nnATC
Cuts at: 6 2091 4128 6
Size: 2085 2037 455
Fragments arranged by size:
2085 2037 455
BsaHI Gr'CG_yC
Cuts at: 2008 2256 2958 4454 2008
Size: 248 702 1496 2131
Fragments arranged by size:
2131 1496 702 248
BsaWI w'CCGG_w
Cuts at: 865 1766 2287 2965 3239 3386 3797 865
Size: 901 521 678 274 147 411 1645
Fragments arranged by size:
1645 901 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2002 2760 3337 3833 2002
Size: 758 577 496 2746
Fragments arranged by size:
2746 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2119 2165 3373 104
Size: 2015 46 1208 1308
Fragments arranged by size:
2015 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1591 2369 2559 3351 1591
Size: 778 190 792 2817
Fragments arranged by size:
2817 792 778 190
BsmI GAATG_Cn'
Cuts at: 385 805 1115 385
Size: 420 310 3847
Fragments arranged by size:
3847 420 310
Bsp1286I G_dGCh'C
Cuts at: 1591 2202 2295 2369 2559 2621 3351 1591
Size: 611 93 74 190 62 730 2817
Fragments arranged by size:
2817 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3797 3797
Size: 4577
BspHI T'CATG_A
Cuts at: 3753 3753
Size: 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1627 1841 2143 2524 1627
Size: 214 302 381 3680
Fragments arranged by size:
3680 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2869 2923 4407 4572 50
Size: 2819 54 1484 165 55
Fragments arranged by size:
2819 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2489 2489
Size: 4577
BsrFI r'CCGG_y
Cuts at: 865 2575 2756 2965 865
Size: 1710 181 209 2477
Fragments arranged by size:
2477 1710 209 181
BssSI C'ACGA_G
Cuts at: 2848 3206 2848
Size: 358 4219
BstAPI GCAn_nnn'nTGC
Cuts at: 421 1954 421
Size: 1533 3044
BstBI TT'CG_AA
Cuts at: 2938 2938
Size: 4577
BstYI r'GATC_y
Cuts at: 1 193 1005 1602 2069 2092 2427 2673 3685
Size: 192 812 597 467 23 335 246 1012
Cuts at: 3685 3771 3783 4129 1
Size: 86 12 346 449
Fragments arranged by size:
1012 812 597 467 449 346 335 246
192 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4577
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2527 2708 2871 2527
Size: 181 163 4233
Fragments arranged by size:
4233 181 163
BtsI GCAGTG_nn'
Cuts at: 750 834 1820 750
Size: 84 986 3507
Fragments arranged by size:
3507 986 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 372 407 792 827 372
Size: 35 385 35 4122
Fragments arranged by size:
4122 385 35 35
DraI TTT'AAA
Cuts at: 1576 1576
Size: 4577
DrdI GACnn_nn'nnGTC
Cuts at: 2283 3141 2283
Size: 858 3719
EaeI y'GGCC_r
Cuts at: 2162 2336 2727 2754 4099 2162
Size: 174 391 27 1345 2640
Fragments arranged by size:
2640 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2162 2162
Size: 4577
EarI CTCTTCn'nnn_
Cuts at: 2600 2810 2600
Size: 210 4367
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 786 2785 3095 3241 4387 786
Size: 1999 310 146 1146 976
Fragments arranged by size:
1999 1146 976 310 146
EcoICRI GAG'CTC
Cuts at: 1589 1589
Size: 4577
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 1139 2402 2834 3581 3849 254
Size: 885 1263 432 747 268 982
Fragments arranged by size:
1263 982 885 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1716 1716
Size: 4577
EcoRI G'AATT_C
Cuts at: 269 353 437 521 689 857 941 1025 1193
Size: 84 84 84 168 168 84 84 168
Cuts at: 1193 1445 1581 269
Size: 252 136 3265
Fragments arranged by size:
3265 252 168 168 168 136 84 84
84 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4577
FspI TGC'GCA
Cuts at: 2358 2358
Size: 4577
HaeII r_GCGC'y
Cuts at: 2011 2259 3281 4260 2011
Size: 248 1022 979 2328
Fragments arranged by size:
2328 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 213 245 587 619 2419 2449 2451 2481 213
Size: 32 342 32 1800 30 2 30 2309
Fragments arranged by size:
2309 1800 342 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1616 1616
Size: 4577
KasI G'GCGC_C
Cuts at: 2007 2255 2007
Size: 248 4329
KpnI G_GTAC'C
Cuts at: 1597 1597
Size: 4577
MluI A'CGCG_T
Cuts at: 4010 4010
Size: 4577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1290 3248 3432 1290
Size: 1958 184 2435
Fragments arranged by size:
2435 1958 184
MscI TGG'CCA
Cuts at: 2338 2338
Size: 4577
MslI CAynn'nnrTG
Cuts at: 212 278 362 446 530 614 698 782 866
Size: 66 84 84 84 84 84 84 84
Cuts at: 866 882 950 1034 1202 1286 1370 1454 2566
Size: 16 68 84 168 84 84 84 1112
Cuts at: 2566 2693 212
Size: 127 2096
Fragments arranged by size:
2096 1112 168 127 84 84 84 84
84 84 84 84 84 84 84 68
66 16
MspA1I CmG'CkG
Cuts at: 1688 2002 2362 3375 3620 1688
Size: 314 360 1013 245 2645
Fragments arranged by size:
2645 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2758 2758
Size: 4577
NarI GG'CG_CC
Cuts at: 2008 2256 2008
Size: 248 4329
NcoI C'CATG_G
Cuts at: 107 107
Size: 4577
NgoMIV G'CCGG_C
Cuts at: 2756 2756
Size: 4577
NsiI A_TGCA'T
Cuts at: 1115 1115
Size: 4577
NspI r_CATG'y
Cuts at: 243 812 1630 2130 3037 243
Size: 569 818 500 907 1783
Fragments arranged by size:
1783 907 818 569 500
PciI A'CATG_T
Cuts at: 3033 3033
Size: 4577
PmeI GTTT'AAAC
Cuts at: 1576 1576
Size: 4577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 3716
Size: 32 4545
PstI C_TGCA'G
Cuts at: 1624 2309 1624
Size: 685 3892
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4577
PvuII CAG'CTG
Cuts at: 2002 2362 2002
Size: 360 4217
RsrII CG'GwC_CG
Cuts at: 2772 2772
Size: 4577
SacI G_AGCT'C
Cuts at: 1591 1591
Size: 4577
SalI G'TCGA_C
Cuts at: 1614 1614
Size: 4577
SapI GCTCTTCn'nnn_
Cuts at: 2600 2810 2600
Size: 210 4367
SbfI CC_TGCA'GG
Cuts at: 1624 1624
Size: 4577
ScaI AGT'ACT
Cuts at: 4094 4094
Size: 4577
SfcI C'TryA_G
Cuts at: 33 1620 2305 3298 3489 33
Size: 1587 685 993 191 1121
Fragments arranged by size:
1587 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2009 2257 2009
Size: 248 4329
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
SmaI CCC'GGG
Cuts at: 1599 1599
Size: 4577
SmlI C'TyrA_G
Cuts at: 1422 1732 2647 3139 3401 1422
Size: 310 915 492 262 2598
Fragments arranged by size:
2598 915 492 310 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4577
SphI G_CATG'C
Cuts at: 1630 2130 1630
Size: 500 4077
StuI AGG'CCT
Cuts at: 400 484 587 904 988 1240 400
Size: 84 103 317 84 252 3737
Fragments arranged by size:
3737 317 252 103 84 84
StyI C'CwwG_G
Cuts at: 107 1711 2065 107
Size: 1604 354 2619
Fragments arranged by size:
2619 1604 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2160 2293 2752 2961 2160
Size: 133 459 209 3776
Fragments arranged by size:
3776 459 209 133
TatI w'GTAC_w
Cuts at: 4092 4092
Size: 4577
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4094 4126 4094
Size: 32 4545
Tth111I GACn'n_nGTC
Cuts at: 2374 2374
Size: 4577
XbaI T'CTAG_A
Cuts at: 1608 1608
Size: 4577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2072 2072
Size: 4577
XmaI C'CCGG_G
Cuts at: 1597 1597
Size: 4577
XmnI GAAnn'nnTTC
Cuts at: 1083 1083
Size: 4577
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI