Restriction map for
pF1KB9663
(Circular) (Six-base) MAPSORT of: pF1KB9663.seq Check: 7013 from: 1 to: 3785
pF1KB9663 3785 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 240 823 240
Size: 583 3202
Acc65I G'GTAC_C
Cuts at: 801 801
Size: 3785
AclI AA'CG_TT
Cuts at: 3625 3625
Size: 3785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 1610 2042 2789 3057 219
Size: 1391 432 747 268 947
Fragments arranged by size:
1391 947 747 432 268
AflIII A'CryG_T
Cuts at: 1137 2241 3218 1137
Size: 1104 977 1704
Fragments arranged by size:
1704 1104 977
AgeI A'CCGG_T
Cuts at: 2173 2173
Size: 3785
AlwNI CAG_nnn'CTG
Cuts at: 1090 2657 1090
Size: 1567 2218
ApaLI G'TGCA_C
Cuts at: 2555 2555
Size: 3785
ApoI r'AATT_y
Cuts at: 500 789 3138 3394 500
Size: 289 2349 256 891
Fragments arranged by size:
2349 891 289 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
AvaI C'yCGr_G
Cuts at: 698 805 698
Size: 107 3678
BamHI G'GATC_C
Cuts at: 416 810 416
Size: 394 3391
BanI G'GyrC_C
Cuts at: 801 1215 1463 1498 801
Size: 414 248 35 3088
Fragments arranged by size:
3088 414 248 35
BanII G_rGCy'C
Cuts at: 148 478 562 646 799 1829 148
Size: 330 84 84 153 1030 2104
Fragments arranged by size:
2104 1030 330 153 84 84
BbeI G_GCGC'C
Cuts at: 1219 1467 1219
Size: 248 3537
BbsI GAAGACnn'nnnn_
Cuts at: 727 727
Size: 3785
BciVI GTATCCnnnnn_n'
Cuts at: 1683 2444 3785 1683
Size: 761 1341 1683
Fragments arranged by size:
1683 1341 761
BclI T'GATC_A
Cuts at: 1305 1305
Size: 3785
BglII A'GATC_T
Cuts at: 1300 3337 1300
Size: 2037 1748
BlpI GC'TnA_GC
Cuts at: 897 897
Size: 3785
Bme1580I G_kGCm'C
Cuts at: 1410 1503 2559 1410
Size: 93 1056 2636
Fragments arranged by size:
2636 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 602 997 1078 1413 3557 602
Size: 395 81 335 2144 830
Fragments arranged by size:
2144 830 395 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 329 2152 329
Size: 1823 1962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 530
Size: 3785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 213 961 1840 2332 2630 132
Size: 81 748 879 492 298 1287
Fragments arranged by size:
1287 879 748 492 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 778 3489 778
Size: 2711 1074
BsaAI yAC'GTr
Cuts at: 1138 1138
Size: 3785
BsaBI GATnn'nnATC
Cuts at: 6 1299 3336 6
Size: 1293 2037 455
Fragments arranged by size:
2037 1293 455
BsaHI Gr'CG_yC
Cuts at: 1216 1464 2166 3662 1216
Size: 248 702 1496 1339
Fragments arranged by size:
1496 1339 702 248
BsaWI w'CCGG_w
Cuts at: 974 1495 2173 2447 2594 3005 974
Size: 521 678 274 147 411 1754
Fragments arranged by size:
1754 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1210 1968 2545 3041 1210
Size: 758 577 496 1954
Fragments arranged by size:
1954 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 633 633
Size: 3785
BsiEI CG_ry'CG
Cuts at: 104 1327 1373 2581 104
Size: 1223 46 1208 1308
Fragments arranged by size:
1308 1223 1208 46
BsiHKAI G_wGCw'C
Cuts at: 148 478 562 646 799 1577 1767 2559 148
Size: 330 84 84 153 778 190 792 1374
Fragments arranged by size:
1374 792 778 330 190 153 84 84
Bsp1286I G_dGCh'C
Cuts at: 148 478 562 646 799 1410 1503 1577 1767
Size: 330 84 84 153 611 93 74 190
Cuts at: 1767 1829 2559 148
Size: 62 730 1374
Fragments arranged by size:
1374 730 611 330 190 153 93 84
84 74 62
BspEI T'CCGG_A
Cuts at: 3005 3005
Size: 3785
BspHI T'CATG_A
Cuts at: 2961 2961
Size: 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 835 1049 1351 1732 835
Size: 214 302 381 2888
Fragments arranged by size:
2888 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2077 2131 3615 3780 50
Size: 2027 54 1484 165 55
Fragments arranged by size:
2027 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1697 1697
Size: 3785
BsrFI r'CCGG_y
Cuts at: 188 542 1783 1964 2173 188
Size: 354 1241 181 209 1800
Fragments arranged by size:
1800 1241 354 209 181
BssSI C'ACGA_G
Cuts at: 2056 2414 2056
Size: 358 3427
BstAPI GCAn_nnn'nTGC
Cuts at: 1162 1162
Size: 3785
BstBI TT'CG_AA
Cuts at: 2146 2146
Size: 3785
BstYI r'GATC_y
Cuts at: 1 332 416 810 1277 1300 1635 1881 2893
Size: 331 84 394 467 23 335 246 1012
Cuts at: 2893 2979 2991 3337 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 394 346 335 331 246
86 84 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1735 1916 2079 1735
Size: 181 163 3441
Fragments arranged by size:
3441 181 163
BtsI GCAGTG_nn'
Cuts at: 621 1028 621
Size: 407 3378
DraI TTT'AAA
Cuts at: 784 784
Size: 3785
DrdI GACnn_nn'nnGTC
Cuts at: 1491 2349 1491
Size: 858 2927
EaeI y'GGCC_r
Cuts at: 540 1370 1544 1935 1962 3307 540
Size: 830 174 391 27 1345 1018
Fragments arranged by size:
1345 1018 830 391 174 27
EagI C'GGCC_G
Cuts at: 1370 1370
Size: 3785
EarI CTCTTCn'nnn_
Cuts at: 674 1808 2018 674
Size: 1134 210 2441
Fragments arranged by size:
2441 1134 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1993 2303 2449 3595 1993
Size: 310 146 1146 2183
Fragments arranged by size:
2183 1146 310 146
EcoICRI GAG'CTC
Cuts at: 146 476 560 644 797 146
Size: 330 84 84 153 3134
Fragments arranged by size:
3134 330 153 84 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 530 1610 2042 2789 3057 219
Size: 311 1080 432 747 268 947
Fragments arranged by size:
1080 947 747 432 311 268
EcoO109I rG'GnC_Cy
Cuts at: 924 924
Size: 3785
EcoRI G'AATT_C
Cuts at: 500 789 500
Size: 289 3496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 744 1804 744
Size: 1060 2725
FspI TGC'GCA
Cuts at: 1566 1566
Size: 3785
HaeII r_GCGC'y
Cuts at: 1219 1467 2489 3468 1219
Size: 248 1022 979 1536
Fragments arranged by size:
1536 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 285 317 381 413 465 497 633 665 1627
Size: 32 64 32 52 32 136 32 962
Cuts at: 1627 1657 1659 1689 285
Size: 30 2 30 2381
Fragments arranged by size:
2381 962 136 64 52 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 824 824
Size: 3785
HindIII A'AGCT_T
Cuts at: 231 252 462 231
Size: 21 210 3554
Fragments arranged by size:
3554 210 21
KasI G'GCGC_C
Cuts at: 1215 1463 1215
Size: 248 3537
KpnI G_GTAC'C
Cuts at: 805 805
Size: 3785
MluI A'CGCG_T
Cuts at: 3218 3218
Size: 3785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2456 2640 2456
Size: 184 3601
MscI TGG'CCA
Cuts at: 1546 1546
Size: 3785
MslI CAynn'nnrTG
Cuts at: 359 667 1774 1901 359
Size: 308 1107 127 2243
Fragments arranged by size:
2243 1107 308 127
MspA1I CmG'CkG
Cuts at: 195 896 1210 1570 2583 2828 195
Size: 701 314 360 1013 245 1152
Fragments arranged by size:
1152 1013 701 360 314 245
NaeI GCC'GGC
Cuts at: 190 1966 190
Size: 1776 2009
NarI GG'CG_CC
Cuts at: 1216 1464 1216
Size: 248 3537
NgoMIV G'CCGG_C
Cuts at: 188 1964 188
Size: 1776 2009
NspI r_CATG'y
Cuts at: 838 1338 2245 838
Size: 500 907 2378
Fragments arranged by size:
2378 907 500
PciI A'CATG_T
Cuts at: 2241 2241
Size: 3785
PmeI GTTT'AAAC
Cuts at: 784 784
Size: 3785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 3753
PstI C_TGCA'G
Cuts at: 832 1517 832
Size: 685 3100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3785
PvuII CAG'CTG
Cuts at: 1210 1570 1210
Size: 360 3425
RsrII CG'GwC_CG
Cuts at: 1980 1980
Size: 3785
SacI G_AGCT'C
Cuts at: 148 478 562 646 799 148
Size: 330 84 84 153 3134
Fragments arranged by size:
3134 330 153 84 84
SalI G'TCGA_C
Cuts at: 822 822
Size: 3785
SapI GCTCTTCn'nnn_
Cuts at: 1808 2018 1808
Size: 210 3575
SbfI CC_TGCA'GG
Cuts at: 832 832
Size: 3785
ScaI AGT'ACT
Cuts at: 3302 3302
Size: 3785
SfcI C'TryA_G
Cuts at: 33 828 1513 2506 2697 33
Size: 795 685 993 191 1121
Fragments arranged by size:
1121 993 795 685 191
SfoI GGC'GCC
Cuts at: 1217 1465 1217
Size: 248 3537
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
SmaI CCC'GGG
Cuts at: 700 807 700
Size: 107 3678
SmlI C'TyrA_G
Cuts at: 147 228 940 1855 2347 2609 147
Size: 81 712 915 492 262 1323
Fragments arranged by size:
1323 915 712 492 262 81
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3785
SphI G_CATG'C
Cuts at: 838 1338 838
Size: 500 3285
StuI AGG'CCT
Cuts at: 163 163
Size: 3785
StyI C'CwwG_G
Cuts at: 153 919 1273 153
Size: 766 354 2665
Fragments arranged by size:
2665 766 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1368 1501 1960 2169 1368
Size: 133 459 209 2984
Fragments arranged by size:
2984 459 209 133
TatI w'GTAC_w
Cuts at: 277 3300 277
Size: 3023 762
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 3753
Tth111I GACn'n_nGTC
Cuts at: 1582 1582
Size: 3785
XbaI T'CTAG_A
Cuts at: 816 816
Size: 3785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1280 1280
Size: 3785
XmaI C'CCGG_G
Cuts at: 698 805 698
Size: 107 3678
XmnI GAAnn'nnTTC
Cuts at: 379 379
Size: 3785
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI