Restriction map for
pF1KB9666
(Circular) (Six-base) MAPSORT of: pF1KB9666.seq Check: 8929 from: 1 to: 4556
pF1KB9666 4556 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 614 698 866 950 614
Size: 84 168 84 4220
Fragments arranged by size:
4220 168 84 84
AccI GT'mk_AC
Cuts at: 1594 1594
Size: 4556
Acc65I G'GTAC_C
Cuts at: 1572 1572
Size: 4556
AclI AA'CG_TT
Cuts at: 4396 4396
Size: 4556
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 2381 2813 3560 3828 393
Size: 1988 432 747 268 1121
Fragments arranged by size:
1988 1121 747 432 268
AfeI AGC'GCT
Cuts at: 929 929
Size: 4556
AflIII A'CryG_T
Cuts at: 1908 3012 3989 1908
Size: 1104 977 2475
Fragments arranged by size:
2475 1104 977
AgeI A'CCGG_T
Cuts at: 2944 2944
Size: 4556
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1058 1058
Size: 4556
AlwNI CAG_nnn'CTG
Cuts at: 606 942 1051 1405 1861 3428 606
Size: 336 109 354 456 1567 1734
Fragments arranged by size:
1734 1567 456 354 336 109
ApaLI G'TGCA_C
Cuts at: 3326 3326
Size: 4556
ApoI r'AATT_y
Cuts at: 1560 3909 4165 1560
Size: 2349 256 1951
Fragments arranged by size:
2349 1951 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4556
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
AvaI C'yCGr_G
Cuts at: 1105 1576 1105
Size: 471 4085
BamHI G'GATC_C
Cuts at: 1263 1581 1263
Size: 318 4238
BanI G'GyrC_C
Cuts at: 418 1210 1572 1986 2234 2269 418
Size: 792 362 414 248 35 2705
Fragments arranged by size:
2705 792 414 362 248 35
BanII G_rGCy'C
Cuts at: 673 757 841 925 1570 2600 673
Size: 84 84 84 645 1030 2629
Fragments arranged by size:
2629 1030 645 84 84 84
BbeI G_GCGC'C
Cuts at: 1990 2238 1990
Size: 248 4308
BbsI GAAGACnn'nnnn_
Cuts at: 104 104
Size: 4556
BbvCI CC'TCA_GC
Cuts at: 184 1201 184
Size: 1017 3539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 474 508 558 592 726 760 810 844 474
Size: 34 50 34 134 34 50 34 4186
Fragments arranged by size:
4186 134 50 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2454 3215 4556 2454
Size: 761 1341 2454
Fragments arranged by size:
2454 1341 761
BclI T'GATC_A
Cuts at: 2076 2076
Size: 4556
BglI GCCn_nnn'nGGC
Cuts at: 427 427
Size: 4556
BglII A'GATC_T
Cuts at: 2071 4108 2071
Size: 2037 2519
BlpI GC'TnA_GC
Cuts at: 343 431 924 1668 343
Size: 88 493 744 3231
Fragments arranged by size:
3231 744 493 88
Bme1580I G_kGCm'C
Cuts at: 584 643 1255 1346 1370 1394 1418 1533 2181
Size: 59 612 91 24 24 24 115 648
Cuts at: 2181 2274 3330 584
Size: 93 1056 1810
Fragments arranged by size:
1810 1056 648 612 115 93 91 59
24 24 24
BmrI ACTGGGnnnn_n'
Cuts at: 461 1768 1849 2184 4328 461
Size: 1307 81 335 2144 689
Fragments arranged by size:
2144 1307 689 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2923 2923
Size: 4556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 993 1275 919
Size: 74 282 4200
Fragments arranged by size:
4200 282 74
Bpu10I CC'TnA_GC
Cuts at: 184 1117 1201 184
Size: 933 84 3539
Fragments arranged by size:
3539 933 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1732 2611 3103 3401 1732
Size: 879 492 298 2887
Fragments arranged by size:
2887 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1155 4260 1155
Size: 3105 1451
BsaAI yAC'GTr
Cuts at: 948 1909 948
Size: 961 3595
BsaBI GATnn'nnATC
Cuts at: 6 2070 4107 6
Size: 2064 2037 455
Fragments arranged by size:
2064 2037 455
BsaHI Gr'CG_yC
Cuts at: 1987 2235 2937 4433 1987
Size: 248 702 1496 2110
Fragments arranged by size:
2110 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1745 2266 2944 3218 3365 3776 1745
Size: 521 678 274 147 411 2525
Fragments arranged by size:
2525 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 199 229 199
Size: 30 4526
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 207 207
Size: 4556
BseYI C'CCAG_C
Cuts at: 1267 1303 1339 1369 1393 1417 1482 1536 1981
Size: 36 36 30 24 24 65 54 445
Cuts at: 1981 2739 3316 3812 1267
Size: 758 577 496 2011
Fragments arranged by size:
2011 758 577 496 445 65 54 36
36 30 24 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1064 928
Size: 136 4420
BsiEI CG_ry'CG
Cuts at: 104 463 791 1070 1091 2098 2144 3352 104
Size: 359 328 279 21 1007 46 1208 1308
Fragments arranged by size:
1308 1208 1007 359 328 279 46 21
BsiHKAI G_wGCw'C
Cuts at: 269 757 841 925 1184 1570 2348 2538 3330
Size: 488 84 84 259 386 778 190 792
Cuts at: 3330 269
Size: 1495
Fragments arranged by size:
1495 792 778 488 386 259 190 84
84
BsmI GAATG_Cn'
Cuts at: 951 951
Size: 4556
Bsp1286I G_dGCh'C
Cuts at: 269 584 643 673 757 841 925 1184 1255
Size: 315 59 30 84 84 84 259 71
Cuts at: 1255 1346 1370 1394 1418 1533 1570 2181 2274
Size: 91 24 24 24 115 37 611 93
Cuts at: 2274 2348 2538 2600 3330 269
Size: 74 190 62 730 1495
Fragments arranged by size:
1495 730 611 315 259 190 115 93
91 84 84 84 74 71 62 59
37 30 24 24 24
BspEI T'CCGG_A
Cuts at: 3776 3776
Size: 4556
BspHI T'CATG_A
Cuts at: 3732 3732
Size: 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 614 698 866 950 1137 1606 1820 2122 2503
Size: 84 168 84 187 469 214 302 381
Cuts at: 2503 614
Size: 2667
Fragments arranged by size:
2667 469 381 302 214 187 168 84
84
BsrBI CCG'CTC
Cuts at: 50 711 879 2848 2902 4386 4551 50
Size: 661 168 1969 54 1484 165 55
Fragments arranged by size:
1969 1484 661 168 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2468 2468
Size: 4556
BsrFI r'CCGG_y
Cuts at: 1093 1495 2554 2735 2944 1093
Size: 402 1059 181 209 2705
Fragments arranged by size:
2705 1059 402 209 181
BssSI C'ACGA_G
Cuts at: 1466 2827 3185 1466
Size: 1361 358 2837
Fragments arranged by size:
2837 1361 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1051 1933 1051
Size: 882 3674
BstBI TT'CG_AA
Cuts at: 2917 2917
Size: 4556
BstYI r'GATC_y
Cuts at: 1 1263 1294 1581 2048 2071 2406 2652 3664
Size: 1262 31 287 467 23 335 246 1012
Cuts at: 3664 3750 3762 4108 1
Size: 86 12 346 449
Fragments arranged by size:
1262 1012 467 449 346 335 287 246
86 31 23 12
BtgI C'CryG_G
Cuts at: 260 414 438 625 1064 260
Size: 154 24 187 439 3752
Fragments arranged by size:
3752 439 187 154 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 337 505 2506 2687 2850 337
Size: 168 2001 181 163 2043
Fragments arranged by size:
2043 2001 181 168 163
BtsI GCAGTG_nn'
Cuts at: 227 552 736 813 897 1531 1799 227
Size: 325 184 77 84 634 268 2984
Fragments arranged by size:
2984 634 325 268 184 84 77
DraI TTT'AAA
Cuts at: 1555 1555
Size: 4556
DrdI GACnn_nn'nnGTC
Cuts at: 2262 3120 2262
Size: 858 3698
EaeI y'GGCC_r
Cuts at: 248 460 663 788 915 999 1067 1088 1221
Size: 212 203 125 127 84 68 21 133
Cuts at: 1221 2141 2315 2706 2733 4078 248
Size: 920 174 391 27 1345 726
Fragments arranged by size:
1345 920 726 391 212 203 174 133
127 125 84 68 27 21
EagI C'GGCC_G
Cuts at: 460 788 1067 1088 2141 460
Size: 328 279 21 1053 2875
Fragments arranged by size:
2875 1053 328 279 21
EarI CTCTTCn'nnn_
Cuts at: 2579 2789 2579
Size: 210 4346
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 316 2764 3074 3220 4366 316
Size: 2448 310 146 1146 506
Fragments arranged by size:
2448 1146 506 310 146
EcoICRI GAG'CTC
Cuts at: 755 839 923 1568 755
Size: 84 84 645 3743
Fragments arranged by size:
3743 645 84 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 919 993 1275 2381 2813 3560 3828 393
Size: 526 74 282 1106 432 747 268 1121
Fragments arranged by size:
1121 1106 747 526 432 282 268 74
EcoO109I rG'GnC_Cy
Cuts at: 1162 1540 1695 1162
Size: 378 155 4023
Fragments arranged by size:
4023 378 155
EcoRI G'AATT_C
Cuts at: 1560 1560
Size: 4556
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 4556
FspI TGC'GCA
Cuts at: 610 694 862 2337 610
Size: 84 168 1475 2829
Fragments arranged by size:
2829 1475 168 84
HaeII r_GCGC'y
Cuts at: 504 763 798 931 1990 2238 3260 4239 504
Size: 259 35 133 1059 248 1022 979 821
Fragments arranged by size:
1059 1022 979 821 259 248 133 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 150 182 199 231 336 368 1225 1255 1257
Size: 32 17 32 105 32 857 30 2
Cuts at: 1257 1287 2398 2428 2430 2460 150
Size: 30 1111 30 2 30 2246
Fragments arranged by size:
2246 1111 857 105 32 32 32 30
30 30 30 17 2 2
HincII GTy'rAC
Cuts at: 1595 1595
Size: 4556
KasI G'GCGC_C
Cuts at: 1986 2234 1986
Size: 248 4308
KpnI G_GTAC'C
Cuts at: 1576 1576
Size: 4556
MluI A'CGCG_T
Cuts at: 3989 3989
Size: 4556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 866 3227 3411 866
Size: 2361 184 2011
Fragments arranged by size:
2361 2011 184
MscI TGG'CCA
Cuts at: 250 2317 250
Size: 2067 2489
MslI CAynn'nnrTG
Cuts at: 1040 2545 2672 1040
Size: 1505 127 2924
Fragments arranged by size:
2924 1505 127
MspA1I CmG'CkG
Cuts at: 262 368 386 536 627 806 1051 1060 1066
Size: 106 18 150 91 179 245 9 6
Cuts at: 1066 1072 1078 1087 1667 1981 2341 3354 3599
Size: 6 6 9 580 314 360 1013 245
Cuts at: 3599 262
Size: 1219
Fragments arranged by size:
1219 1013 580 360 314 245 245 179
150 106 91 18 9 9 6 6
6
NaeI GCC'GGC
Cuts at: 1095 2737 1095
Size: 1642 2914
NarI GG'CG_CC
Cuts at: 1987 2235 1987
Size: 248 4308
NgoMIV G'CCGG_C
Cuts at: 1093 2735 1093
Size: 1642 2914
NotI GC'GGCC_GC
Cuts at: 1067 1088 1067
Size: 21 4535
NspI r_CATG'y
Cuts at: 1609 2109 3016 1609
Size: 500 907 3149
Fragments arranged by size:
3149 907 500
PciI A'CATG_T
Cuts at: 3012 3012
Size: 4556
PflMI CCAn_nnn'nTGG
Cuts at: 246 1339 246
Size: 1093 3463
PmeI GTTT'AAAC
Cuts at: 1555 1555
Size: 4556
PmlI CAC'GTG
Cuts at: 948 948
Size: 4556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4524
PpuMI rG'GwC_Cy
Cuts at: 1162 1162
Size: 4556
PshAI GACnn'nnGTC
Cuts at: 1352 1352
Size: 4556
PstI C_TGCA'G
Cuts at: 770 1059 1077 1083 1603 2288 770
Size: 289 18 6 520 685 3038
Fragments arranged by size:
3038 685 520 289 18 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4556
PvuII CAG'CTG
Cuts at: 1060 1078 1981 2341 1060
Size: 18 903 360 3275
Fragments arranged by size:
3275 903 360 18
RsrII CG'GwC_CG
Cuts at: 2751 2751
Size: 4556
SacI G_AGCT'C
Cuts at: 757 841 925 1570 757
Size: 84 84 645 3743
Fragments arranged by size:
3743 645 84 84
SacII CC_GC'GG
Cuts at: 263 628 1067 263
Size: 365 439 3752
Fragments arranged by size:
3752 439 365
SalI G'TCGA_C
Cuts at: 1593 1593
Size: 4556
SapI GCTCTTCn'nnn_
Cuts at: 2579 2789 2579
Size: 210 4346
SbfI CC_TGCA'GG
Cuts at: 1603 1603
Size: 4556
ScaI AGT'ACT
Cuts at: 4073 4073
Size: 4556
SfcI C'TryA_G
Cuts at: 33 766 1055 1073 1079 1599 2284 3277 3468
Size: 733 289 18 6 520 685 993 191
Cuts at: 3468 33
Size: 1121
Fragments arranged by size:
1121 993 733 685 520 289 191 18
6
SfoI GGC'GCC
Cuts at: 1988 2236 1988
Size: 248 4308
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
SmaI CCC'GGG
Cuts at: 1578 1578
Size: 4556
SmlI C'TyrA_G
Cuts at: 1711 2626 3118 3380 1711
Size: 915 492 262 2887
Fragments arranged by size:
2887 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4556
SphI G_CATG'C
Cuts at: 1609 2109 1609
Size: 500 4056
StuI AGG'CCT
Cuts at: 490 574 742 910 994 1006 490
Size: 84 168 168 84 12 4040
Fragments arranged by size:
4040 168 168 84 84 12
StyI C'CwwG_G
Cuts at: 653 905 1690 2044 653
Size: 252 785 354 3165
Fragments arranged by size:
3165 785 354 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2139 2272 2731 2940 2139
Size: 133 459 209 3755
Fragments arranged by size:
3755 459 209 133
TatI w'GTAC_w
Cuts at: 4071 4071
Size: 4556
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4073 4105 4073
Size: 32 4524
Tth111I GACn'n_nGTC
Cuts at: 2353 2353
Size: 4556
XbaI T'CTAG_A
Cuts at: 1587 1587
Size: 4556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2051 2051
Size: 4556
XmaI C'CCGG_G
Cuts at: 1576 1576
Size: 4556
XmnI GAAnn'nnTTC
Cuts at: 406 994 406
Size: 588 3968
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NotI NspI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NruI NsiI PacI PasI PfoI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI