Restriction map for
pF1KB9668
(Circular) (Six-base) MAPSORT of: pF1KB9668.seq Check: 7204 from: 1 to: 4607
pF1KB9668 4607 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 126 126
Size: 4607
AccI GT'mk_AC
Cuts at: 1645 1645
Size: 4607
Acc65I G'GTAC_C
Cuts at: 1623 1623
Size: 4607
AclI AA'CG_TT
Cuts at: 4447 4447
Size: 4607
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 771 855 922 1191 2432 2864 3611 3879
Size: 83 84 67 269 1241 432 747 268
Cuts at: 3879 688
Size: 1416
Fragments arranged by size:
1416 1241 747 432 269 268 84 83
67
AfeI AGC'GCT
Cuts at: 1216 1216
Size: 4607
AflII C'TTAA_G
Cuts at: 1479 1479
Size: 4607
AflIII A'CryG_T
Cuts at: 509 1959 3063 4040 509
Size: 1450 1104 977 1076
Fragments arranged by size:
1450 1104 1076 977
AgeI A'CCGG_T
Cuts at: 2995 2995
Size: 4607
AleI CACnn'nnGTG
Cuts at: 721 1082 721
Size: 361 4246
AlwNI CAG_nnn'CTG
Cuts at: 1059 1410 1912 3479 1059
Size: 351 502 1567 2187
Fragments arranged by size:
2187 1567 502 351
ApaI G_GGCC'C
Cuts at: 334 365 334
Size: 31 4576
ApaLI G'TGCA_C
Cuts at: 487 823 1159 3377 487
Size: 336 336 2218 1717
Fragments arranged by size:
2218 1717 336 336
ApoI r'AATT_y
Cuts at: 1611 3960 4216 1611
Size: 2349 256 2002
Fragments arranged by size:
2349 2002 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4607
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4607
AvaI C'yCGr_G
Cuts at: 517 1248 1627 517
Size: 731 379 3497
Fragments arranged by size:
3497 731 379
BamHI G'GATC_C
Cuts at: 1632 1632
Size: 4607
BanI G'GyrC_C
Cuts at: 1252 1623 2037 2285 2320 1252
Size: 371 414 248 35 3539
Fragments arranged by size:
3539 414 371 248 35
BanII G_rGCy'C
Cuts at: 334 365 463 547 883 1051 1135 1478 1621
Size: 31 98 84 336 168 84 343 143
Cuts at: 1621 2651 334
Size: 1030 2290
Fragments arranged by size:
2290 1030 343 336 168 143 98 84
84 31
BbeI G_GCGC'C
Cuts at: 1256 2041 2289 1256
Size: 785 248 3574
Fragments arranged by size:
3574 785 248
BbsI GAAGACnn'nnnn_
Cuts at: 181 181
Size: 4607
BbvCI CC'TCA_GC
Cuts at: 553 553
Size: 4607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 516 550 684 718 936 970 1440 1474 516
Size: 34 134 34 218 34 470 34 3649
Fragments arranged by size:
3649 470 218 134 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2505 3266 4607 2505
Size: 761 1341 2505
Fragments arranged by size:
2505 1341 761
BclI T'GATC_A
Cuts at: 2127 2127
Size: 4607
BglII A'GATC_T
Cuts at: 2122 4159 2122
Size: 2037 2570
BlpI GC'TnA_GC
Cuts at: 1719 1719
Size: 4607
Bme1580I G_kGCm'C
Cuts at: 334 365 491 517 769 827 1163 2232 2325
Size: 31 126 26 252 58 336 1069 93
Cuts at: 2325 3381 334
Size: 1056 1560
Fragments arranged by size:
1560 1069 1056 336 252 126 93 58
31 26
BmrI ACTGGGnnnn_n'
Cuts at: 298 1185 1819 1900 2235 4379 298
Size: 887 634 81 335 2144 526
Fragments arranged by size:
2144 887 634 526 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2974 2974
Size: 4607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 561 829 233
Size: 328 268 4011
Fragments arranged by size:
4011 328 268
Bpu10I CC'TnA_GC
Cuts at: 553 553
Size: 4607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1783 2662 3154 3452 1783
Size: 879 492 298 2938
Fragments arranged by size:
2938 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4311 4311
Size: 4607
BsaAI yAC'GTr
Cuts at: 512 1960 512
Size: 1448 3159
BsaBI GATnn'nnATC
Cuts at: 6 2121 4158 6
Size: 2115 2037 455
Fragments arranged by size:
2115 2037 455
BsaHI Gr'CG_yC
Cuts at: 1253 2038 2286 2988 4484 1253
Size: 785 248 702 1496 1376
Fragments arranged by size:
1496 1376 785 702 248
BsaWI w'CCGG_w
Cuts at: 1796 2317 2995 3269 3416 3827 1796
Size: 521 678 274 147 411 2576
Fragments arranged by size:
2576 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 427 427
Size: 4607
BseYI C'CCAG_C
Cuts at: 218 327 358 2032 2790 3367 3863 218
Size: 109 31 1674 758 577 496 962
Fragments arranged by size:
1674 962 758 577 496 109 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 1396 745
Size: 651 3956
BsiEI CG_ry'CG
Cuts at: 104 708 841 2149 2195 3403 104
Size: 604 133 1308 46 1208 1308
Fragments arranged by size:
1308 1308 1208 604 133 46
BsiHKAI G_wGCw'C
Cuts at: 491 547 815 827 883 899 1051 1151 1163
Size: 56 268 12 56 16 152 100 12
Cuts at: 1163 1478 1571 1621 2399 2589 3381 491
Size: 315 93 50 778 190 792 1717
Fragments arranged by size:
1717 792 778 315 268 190 152 100
93 56 56 50 16 12 12
BsmI GAATG_Cn'
Cuts at: 1366 1366
Size: 4607
Bsp1286I G_dGCh'C
Cuts at: 334 365 463 491 517 547 769 815 827
Size: 31 98 28 26 30 222 46 12
Cuts at: 827 883 899 1051 1135 1151 1163 1478 1571
Size: 56 16 152 84 16 12 315 93
Cuts at: 1571 1621 2232 2325 2399 2589 2651 3381 334
Size: 50 611 93 74 190 62 730 1560
Fragments arranged by size:
1560 730 611 315 222 190 152 98
93 93 84 74 62 56 50 46
31 30 28 26 16 16 12 12
BspEI T'CCGG_A
Cuts at: 3827 3827
Size: 4607
BspHI T'CATG_A
Cuts at: 3783 3783
Size: 4607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 126 1657 1871 2173 2554 126
Size: 1531 214 302 381 2179
Fragments arranged by size:
2179 1531 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1089 2899 2953 4437 4602 50
Size: 1039 1810 54 1484 165 55
Fragments arranged by size:
1810 1484 1039 165 55 54
BsrDI GCAATG_nn'
Cuts at: 174 2519 174
Size: 2345 2262
BsrFI r'CCGG_y
Cuts at: 425 815 983 1151 1235 1319 1487 2605 2786
Size: 390 168 168 84 84 168 1118 181
Cuts at: 2786 2995 425
Size: 209 2037
Fragments arranged by size:
2037 1118 390 209 181 168 168 168
84 84
BssHII G'CGCG_C
Cuts at: 623 833 623
Size: 210 4397
BssSI C'ACGA_G
Cuts at: 183 2878 3236 183
Size: 2695 358 1554
Fragments arranged by size:
2695 1554 358
BstAPI GCAn_nnn'nTGC
Cuts at: 605 1059 1984 605
Size: 454 925 3228
Fragments arranged by size:
3228 925 454
BstBI TT'CG_AA
Cuts at: 2968 2968
Size: 4607
BstXI CCAn_nnnn'nTGG
Cuts at: 125 807 125
Size: 682 3925
BstYI r'GATC_y
Cuts at: 1 905 1632 2099 2122 2457 2703 3715 3801
Size: 904 727 467 23 335 246 1012 86
Cuts at: 3801 3813 4159 1
Size: 12 346 449
Fragments arranged by size:
1012 904 727 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 239 1293 1377 107
Size: 132 1054 84 3337
Fragments arranged by size:
3337 1054 132 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2557 2738 2901 2557
Size: 181 163 4263
Fragments arranged by size:
4263 181 163
BtsI GCAGTG_nn'
Cuts at: 1443 1850 1443
Size: 407 4200
DraI TTT'AAA
Cuts at: 1606 1606
Size: 4607
DrdI GACnn_nn'nnGTC
Cuts at: 2313 3171 2313
Size: 858 3749
EaeI y'GGCC_r
Cuts at: 267 705 838 890 922 1113 2192 2366 2757
Size: 438 133 52 32 191 1079 174 391
Cuts at: 2757 2784 4129 267
Size: 27 1345 745
Fragments arranged by size:
1345 1079 745 438 391 191 174 133
52 32 27
EagI C'GGCC_G
Cuts at: 705 838 2192 705
Size: 133 1354 3120
Fragments arranged by size:
3120 1354 133
EarI CTCTTCn'nnn_
Cuts at: 2630 2840 2630
Size: 210 4397
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 313 1159 2815 3125 3271 4417 313
Size: 846 1656 310 146 1146 503
Fragments arranged by size:
1656 1146 846 503 310 146
EcoICRI GAG'CTC
Cuts at: 545 881 1049 1476 1619 545
Size: 336 168 427 143 3533
Fragments arranged by size:
3533 427 336 168 143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 561 688 771 829 855 922 1191 2432
Size: 328 127 83 58 26 67 269 1241
Cuts at: 2432 2864 3611 3879 233
Size: 432 747 268 961
Fragments arranged by size:
1241 961 747 432 328 269 268 127
83 67 58 26
EcoNI CCTnn'n_nnAGG
Cuts at: 196 196
Size: 4607
EcoO109I rG'GnC_Cy
Cuts at: 159 189 224 313 331 1511 1746 159
Size: 30 35 89 18 1180 235 3020
Fragments arranged by size:
3020 1180 235 89 35 30 18
EcoRI G'AATT_C
Cuts at: 1611 1611
Size: 4607
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 4607
FspI TGC'GCA
Cuts at: 1356 1465 2388 1356
Size: 109 923 3575
Fragments arranged by size:
3575 923 109
HaeII r_GCGC'y
Cuts at: 1218 1256 1410 2041 2289 3311 4290 1218
Size: 38 154 631 248 1022 979 1535
Fragments arranged by size:
1535 1022 979 631 248 154 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2449 2479 2481 2511 2449
Size: 30 2 30 4545
Fragments arranged by size:
4545 30 30 2
HincII GTy'rAC
Cuts at: 1646 1646
Size: 4607
KasI G'GCGC_C
Cuts at: 1252 2037 2285 1252
Size: 785 248 3574
Fragments arranged by size:
3574 785 248
KpnI G_GTAC'C
Cuts at: 1627 1627
Size: 4607
MluI A'CGCG_T
Cuts at: 4040 4040
Size: 4607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 572 908 1244 1451 3278 3462 572
Size: 336 336 207 1827 184 1717
Fragments arranged by size:
1827 1717 336 336 207 184
MscI TGG'CCA
Cuts at: 2368 2368
Size: 4607
MslI CAynn'nnrTG
Cuts at: 721 1082 2596 2723 721
Size: 361 1514 127 2605
Fragments arranged by size:
2605 1514 361 127
MspA1I CmG'CkG
Cuts at: 273 578 662 680 791 1295 1379 1718 2032
Size: 305 84 18 111 504 84 339 314
Cuts at: 2032 2392 3405 3650 273
Size: 360 1013 245 1230
Fragments arranged by size:
1230 1013 504 360 339 314 305 245
111 84 84 18
NaeI GCC'GGC
Cuts at: 2788 2788
Size: 4607
NarI GG'CG_CC
Cuts at: 1253 2038 2286 1253
Size: 785 248 3574
Fragments arranged by size:
3574 785 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4607
NgoMIV G'CCGG_C
Cuts at: 2786 2786
Size: 4607
NotI GC'GGCC_GC
Cuts at: 838 838
Size: 4607
NspI r_CATG'y
Cuts at: 1660 2160 3067 1660
Size: 500 907 3200
Fragments arranged by size:
3200 907 500
PciI A'CATG_T
Cuts at: 3063 3063
Size: 4607
PfoI T'CCnGG_A
Cuts at: 806 806
Size: 4607
PmeI GTTT'AAAC
Cuts at: 1606 1606
Size: 4607
PmlI CAC'GTG
Cuts at: 512 512
Size: 4607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1414 1446 3746 3778 1414
Size: 32 2300 32 2243
Fragments arranged by size:
2300 2243 32 32
PpuMI rG'GwC_Cy
Cuts at: 159 159
Size: 4607
PspOMI G'GGCC_C
Cuts at: 330 361 330
Size: 31 4576
PstI C_TGCA'G
Cuts at: 137 381 476 1064 1450 1654 2339 137
Size: 244 95 588 386 204 685 2405
Fragments arranged by size:
2405 685 588 386 244 204 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4607
PvuII CAG'CTG
Cuts at: 791 2032 2392 791
Size: 1241 360 3006
Fragments arranged by size:
3006 1241 360
RsrII CG'GwC_CG
Cuts at: 2802 2802
Size: 4607
SacI G_AGCT'C
Cuts at: 547 883 1051 1478 1621 547
Size: 336 168 427 143 3533
Fragments arranged by size:
3533 427 336 168 143
SacII CC_GC'GG
Cuts at: 1296 1380 1296
Size: 84 4523
SalI G'TCGA_C
Cuts at: 1644 1644
Size: 4607
SapI GCTCTTCn'nnn_
Cuts at: 2630 2840 2630
Size: 210 4397
SbfI CC_TGCA'GG
Cuts at: 137 1654 137
Size: 1517 3090
ScaI AGT'ACT
Cuts at: 4124 4124
Size: 4607
SfcI C'TryA_G
Cuts at: 33 133 377 472 1060 1446 1650 2335 3328
Size: 100 244 95 588 386 204 685 993
Cuts at: 3328 3519 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 588 386 244 204 191
100 95
SfoI GGC'GCC
Cuts at: 1254 2039 2287 1254
Size: 785 248 3574
Fragments arranged by size:
3574 785 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4607
SgrAI Cr'CCGG_yG
Cuts at: 815 983 1151 1235 1487 815
Size: 168 168 84 252 3935
Fragments arranged by size:
3935 252 168 168 84
SmaI CCC'GGG
Cuts at: 1629 1629
Size: 4607
SmlI C'TyrA_G
Cuts at: 1479 1762 2677 3169 3431 1479
Size: 283 915 492 262 2655
Fragments arranged by size:
2655 915 492 283 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4607
SphI G_CATG'C
Cuts at: 1660 2160 1660
Size: 500 4107
StuI AGG'CCT
Cuts at: 532 616 784 868 952 1204 1288 1372 1456
Size: 84 168 84 84 252 84 84 84
Cuts at: 1456 532
Size: 3683
Fragments arranged by size:
3683 252 168 84 84 84 84 84
84
StyI C'CwwG_G
Cuts at: 107 1741 2095 107
Size: 1634 354 2619
Fragments arranged by size:
2619 1634 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2190 2323 2782 2991 2190
Size: 133 459 209 3806
Fragments arranged by size:
3806 459 209 133
TatI w'GTAC_w
Cuts at: 4122 4122
Size: 4607
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 231 263 4124 4156 231
Size: 32 3861 32 682
Fragments arranged by size:
3861 682 32 32
Tth111I GACn'n_nGTC
Cuts at: 2404 2404
Size: 4607
XbaI T'CTAG_A
Cuts at: 1638 1638
Size: 4607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2102 2102
Size: 4607
XmaI C'CCGG_G
Cuts at: 1627 1627
Size: 4607
XmnI GAAnn'nnTTC
Cuts at: 673 1009 1540 673
Size: 336 531 3740
Fragments arranged by size:
3740 531 336
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PciI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AlfI AloI AscI AvrII BaeI BfrBI
BglI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NruI NsiI PacI
PasI PflMI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SnaBI SrfI SspI SwaI XhoI ZraI