Restriction map for
pF1KB9670
(Circular) (Six-base) MAPSORT of: pF1KB9670.seq Check: 5985 from: 1 to: 3932
pF1KB9670 3932 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 377 970 377
Size: 593 3339
Acc65I G'GTAC_C
Cuts at: 948 948
Size: 3932
AclI AA'CG_TT
Cuts at: 3772 3772
Size: 3932
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 516 1757 2189 2936 3204 516
Size: 1241 432 747 268 1244
Fragments arranged by size:
1244 1241 747 432 268
AflIII A'CryG_T
Cuts at: 1284 2388 3365 1284
Size: 1104 977 1851
Fragments arranged by size:
1851 1104 977
AgeI A'CCGG_T
Cuts at: 2320 2320
Size: 3932
AleI CACnn'nnGTG
Cuts at: 325 325
Size: 3932
AlwNI CAG_nnn'CTG
Cuts at: 1237 2804 1237
Size: 1567 2365
ApaLI G'TGCA_C
Cuts at: 2702 2702
Size: 3932
ApoI r'AATT_y
Cuts at: 936 3285 3541 936
Size: 2349 256 1327
Fragments arranged by size:
2349 1327 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3932
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
AvaI C'yCGr_G
Cuts at: 952 952
Size: 3932
BamHI G'GATC_C
Cuts at: 957 957
Size: 3932
BanI G'GyrC_C
Cuts at: 948 1362 1610 1645 948
Size: 414 248 35 3235
Fragments arranged by size:
3235 414 248 35
BanII G_rGCy'C
Cuts at: 115 946 1976 115
Size: 831 1030 2071
Fragments arranged by size:
2071 1030 831
BbeI G_GCGC'C
Cuts at: 1366 1614 1366
Size: 248 3684
BbsI GAAGACnn'nnnn_
Cuts at: 253 438 540 700 253
Size: 185 102 160 3485
Fragments arranged by size:
3485 185 160 102
BciVI GTATCCnnnnn_n'
Cuts at: 1830 2591 3932 1830
Size: 761 1341 1830
Fragments arranged by size:
1830 1341 761
BclI T'GATC_A
Cuts at: 1452 1452
Size: 3932
BglII A'GATC_T
Cuts at: 1447 3484 1447
Size: 2037 1895
BlpI GC'TnA_GC
Cuts at: 1044 1044
Size: 3932
Bme1580I G_kGCm'C
Cuts at: 1557 1650 2706 1557
Size: 93 1056 2783
Fragments arranged by size:
2783 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 332 1144 1225 1560 3704 332
Size: 812 81 335 2144 560
Fragments arranged by size:
2144 812 560 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 192 208 601 2299 192
Size: 16 393 1698 1825
Fragments arranged by size:
1825 1698 393 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 596 680 848 932 512
Size: 84 84 168 84 3512
Fragments arranged by size:
3512 168 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1108 1987 2479 2777 1108
Size: 879 492 298 2263
Fragments arranged by size:
2263 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 180 3636 180
Size: 3456 476
BsaAI yAC'GTr
Cuts at: 1285 1285
Size: 3932
BsaBI GATnn'nnATC
Cuts at: 6 1446 3483 6
Size: 1440 2037 455
Fragments arranged by size:
2037 1440 455
BsaHI Gr'CG_yC
Cuts at: 1363 1611 2313 3809 1363
Size: 248 702 1496 1486
Fragments arranged by size:
1496 1486 702 248
BsaWI w'CCGG_w
Cuts at: 1121 1642 2320 2594 2741 3152 1121
Size: 521 678 274 147 411 1901
Fragments arranged by size:
1901 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1357 2115 2692 3188 1357
Size: 758 577 496 2101
Fragments arranged by size:
2101 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 531 699 133
Size: 398 168 3366
Fragments arranged by size:
3366 398 168
BsiEI CG_ry'CG
Cuts at: 104 1474 1520 2728 104
Size: 1370 46 1208 1308
Fragments arranged by size:
1370 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 946 1724 1914 2706 946
Size: 778 190 792 2172
Fragments arranged by size:
2172 792 778 190
BsmI GAATG_Cn'
Cuts at: 346 346
Size: 3932
Bsp1286I G_dGCh'C
Cuts at: 115 946 1557 1650 1724 1914 1976 2706 115
Size: 831 611 93 74 190 62 730 1341
Fragments arranged by size:
1341 831 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3152 3152
Size: 3932
BspHI T'CATG_A
Cuts at: 3108 3108
Size: 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 162 446 982 1196 1498 1879 162
Size: 284 536 214 302 381 2215
Fragments arranged by size:
2215 536 381 302 284 214
BsrBI CCG'CTC
Cuts at: 50 2224 2278 3762 3927 50
Size: 2174 54 1484 165 55
Fragments arranged by size:
2174 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1844 1844
Size: 3932
BsrFI r'CCGG_y
Cuts at: 1930 2111 2320 1930
Size: 181 209 3542
Fragments arranged by size:
3542 209 181
BssSI C'ACGA_G
Cuts at: 2203 2561 2203
Size: 358 3574
BstAPI GCAn_nnn'nTGC
Cuts at: 686 1309 686
Size: 623 3309
BstBI TT'CG_AA
Cuts at: 2293 2293
Size: 3932
BstXI CCAn_nnnn'nTGG
Cuts at: 352 352
Size: 3932
BstYI r'GATC_y
Cuts at: 1 902 957 1424 1447 1782 2028 3040 3126
Size: 901 55 467 23 335 246 1012 86
Cuts at: 3126 3138 3484 1
Size: 12 346 449
Fragments arranged by size:
1012 901 467 449 346 335 246 86
55 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 137 1882 2063 2226 137
Size: 1745 181 163 1843
Fragments arranged by size:
1843 1745 181 163
BtsI GCAGTG_nn'
Cuts at: 687 740 1175 687
Size: 53 435 3444
Fragments arranged by size:
3444 435 53
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 249 284 585 620 249
Size: 35 301 35 3561
Fragments arranged by size:
3561 301 35 35
DraI TTT'AAA
Cuts at: 931 931
Size: 3932
DrdI GACnn_nn'nnGTC
Cuts at: 1638 2496 1638
Size: 858 3074
EaeI y'GGCC_r
Cuts at: 1517 1691 2082 2109 3454 1517
Size: 174 391 27 1345 1995
Fragments arranged by size:
1995 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1517 1517
Size: 3932
EarI CTCTTCn'nnn_
Cuts at: 633 1955 2165 633
Size: 1322 210 2400
Fragments arranged by size:
2400 1322 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2140 2450 2596 3742 2140
Size: 310 146 1146 2330
Fragments arranged by size:
2330 1146 310 146
EcoICRI GAG'CTC
Cuts at: 944 944
Size: 3932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 516 596 680 848 932 1757 2189 2936
Size: 4 80 84 168 84 825 432 747
Cuts at: 2936 3204 512
Size: 268 1240
Fragments arranged by size:
1240 825 747 432 268 168 84 84
80 4
EcoO109I rG'GnC_Cy
Cuts at: 1071 1071
Size: 3932
EcoRI G'AATT_C
Cuts at: 936 936
Size: 3932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 396 1951 396
Size: 1555 2377
FspI TGC'GCA
Cuts at: 1713 1713
Size: 3932
HaeII r_GCGC'y
Cuts at: 1366 1614 2636 3615 1366
Size: 248 1022 979 1683
Fragments arranged by size:
1683 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1774 1804 1806 1836 1774
Size: 30 2 30 3870
Fragments arranged by size:
3870 30 30 2
HincII GTy'rAC
Cuts at: 378 971 378
Size: 593 3339
KasI G'GCGC_C
Cuts at: 1362 1610 1362
Size: 248 3684
KpnI G_GTAC'C
Cuts at: 952 952
Size: 3932
MluI A'CGCG_T
Cuts at: 3365 3365
Size: 3932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 747 2603 2787 747
Size: 1856 184 1892
Fragments arranged by size:
1892 1856 184
MscI TGG'CCA
Cuts at: 1693 1693
Size: 3932
MslI CAynn'nnrTG
Cuts at: 200 325 1921 2048 200
Size: 125 1596 127 2084
Fragments arranged by size:
2084 1596 127 125
MspA1I CmG'CkG
Cuts at: 1043 1357 1717 2730 2975 1043
Size: 314 360 1013 245 2000
Fragments arranged by size:
2000 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2113 2113
Size: 3932
NarI GG'CG_CC
Cuts at: 1363 1611 1363
Size: 248 3684
NgoMIV G'CCGG_C
Cuts at: 2111 2111
Size: 3932
NspI r_CATG'y
Cuts at: 985 1485 2392 985
Size: 500 907 2525
Fragments arranged by size:
2525 907 500
PciI A'CATG_T
Cuts at: 2388 2388
Size: 3932
PmeI GTTT'AAAC
Cuts at: 931 931
Size: 3932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3071 3103 3071
Size: 32 3900
PstI C_TGCA'G
Cuts at: 747 979 1664 747
Size: 232 685 3015
Fragments arranged by size:
3015 685 232
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3932
PvuII CAG'CTG
Cuts at: 1357 1717 1357
Size: 360 3572
RsrII CG'GwC_CG
Cuts at: 2127 2127
Size: 3932
SacI G_AGCT'C
Cuts at: 946 946
Size: 3932
SalI G'TCGA_C
Cuts at: 376 969 376
Size: 593 3339
SapI GCTCTTCn'nnn_
Cuts at: 1955 2165 1955
Size: 210 3722
SbfI CC_TGCA'GG
Cuts at: 979 979
Size: 3932
ScaI AGT'ACT
Cuts at: 3449 3449
Size: 3932
SfcI C'TryA_G
Cuts at: 33 743 789 975 1660 2653 2844 33
Size: 710 46 186 685 993 191 1121
Fragments arranged by size:
1121 993 710 685 191 186 46
SfoI GGC'GCC
Cuts at: 1364 1612 1364
Size: 248 3684
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
SmaI CCC'GGG
Cuts at: 954 954
Size: 3932
SmlI C'TyrA_G
Cuts at: 1087 2002 2494 2756 1087
Size: 915 492 262 2263
Fragments arranged by size:
2263 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3932
SphI G_CATG'C
Cuts at: 985 1485 985
Size: 500 3432
StuI AGG'CCT
Cuts at: 865 865
Size: 3932
StyI C'CwwG_G
Cuts at: 1066 1420 1066
Size: 354 3578
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 581 1515 1648 2107 2316 581
Size: 934 133 459 209 2197
Fragments arranged by size:
2197 934 459 209 133
TatI w'GTAC_w
Cuts at: 3447 3447
Size: 3932
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 3900
Tth111I GACn'n_nGTC
Cuts at: 1729 1729
Size: 3932
XbaI T'CTAG_A
Cuts at: 963 963
Size: 3932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 487 1427 487
Size: 940 2992
XmaI C'CCGG_G
Cuts at: 952 952
Size: 3932
XmnI GAAnn'nnTTC
Cuts at: 445 697 445
Size: 252 3680
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI