Restriction map for
pF1KB9673
(Circular) (Six-base) MAPSORT of: pF1KB9673.seq Check: 1130 from: 1 to: 3905
pF1KB9673 3905 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 943 943
Size: 3905
Acc65I G'GTAC_C
Cuts at: 921 921
Size: 3905
AclI AA'CG_TT
Cuts at: 3745 3745
Size: 3905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1730 2162 2909 3177 196
Size: 1534 432 747 268 924
Fragments arranged by size:
1534 924 747 432 268
AflIII A'CryG_T
Cuts at: 1257 2361 3338 1257
Size: 1104 977 1824
Fragments arranged by size:
1824 1104 977
AgeI A'CCGG_T
Cuts at: 2293 2293
Size: 3905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 351 351
Size: 3905
AlwNI CAG_nnn'CTG
Cuts at: 1210 2777 1210
Size: 1567 2338
ApaLI G'TGCA_C
Cuts at: 2675 2675
Size: 3905
ApoI r'AATT_y
Cuts at: 272 909 3258 3514 272
Size: 637 2349 256 663
Fragments arranged by size:
2349 663 637 256
AseI AT'TA_AT
Cuts at: 20 749 760 20
Size: 729 11 3165
Fragments arranged by size:
3165 729 11
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
AvaI C'yCGr_G
Cuts at: 925 925
Size: 3905
AvrII C'CTAG_G
Cuts at: 445 445
Size: 3905
BamHI G'GATC_C
Cuts at: 930 930
Size: 3905
BanI G'GyrC_C
Cuts at: 921 1335 1583 1618 921
Size: 414 248 35 3208
Fragments arranged by size:
3208 414 248 35
BanII G_rGCy'C
Cuts at: 919 1949 919
Size: 1030 2875
BbeI G_GCGC'C
Cuts at: 1339 1587 1339
Size: 248 3657
BbvCI CC'TCA_GC
Cuts at: 726 726
Size: 3905
BciVI GTATCCnnnnn_n'
Cuts at: 1803 2564 3905 1803
Size: 761 1341 1803
Fragments arranged by size:
1803 1341 761
BclI T'GATC_A
Cuts at: 345 425 1425 345
Size: 80 1000 2825
Fragments arranged by size:
2825 1000 80
BfrBI ATG'CAT
Cuts at: 835 835
Size: 3905
BglII A'GATC_T
Cuts at: 1420 3457 1420
Size: 2037 1868
BlpI GC'TnA_GC
Cuts at: 1017 1017
Size: 3905
Bme1580I G_kGCm'C
Cuts at: 1530 1623 2679 1530
Size: 93 1056 2756
Fragments arranged by size:
2756 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1117 1198 1533 3677 1117
Size: 81 335 2144 1345
Fragments arranged by size:
2144 1345 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 3905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 680
Size: 3905
Bpu10I CC'TnA_GC
Cuts at: 726 726
Size: 3905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 1960 2452 2750 1081
Size: 879 492 298 2236
Fragments arranged by size:
2236 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3609 3609
Size: 3905
BsaAI yAC'GTr
Cuts at: 1258 1258
Size: 3905
BsaBI GATnn'nnATC
Cuts at: 6 1419 3456 6
Size: 1413 2037 455
Fragments arranged by size:
2037 1413 455
BsaHI Gr'CG_yC
Cuts at: 1336 1584 2286 3782 1336
Size: 248 702 1496 1459
Fragments arranged by size:
1496 1459 702 248
BsaWI w'CCGG_w
Cuts at: 1094 1615 2293 2567 2714 3125 1094
Size: 521 678 274 147 411 1874
Fragments arranged by size:
1874 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 114 144 348 378 114
Size: 30 204 30 3641
Fragments arranged by size:
3641 204 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 353 353
Size: 3905
BseYI C'CCAG_C
Cuts at: 1330 2088 2665 3161 1330
Size: 758 577 496 2074
Fragments arranged by size:
2074 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1447 1493 2701 104
Size: 1343 46 1208 1308
Fragments arranged by size:
1343 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 919 1697 1887 2679 919
Size: 778 190 792 2145
Fragments arranged by size:
2145 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 919 1530 1623 1697 1887 1949 2679 919
Size: 611 93 74 190 62 730 2145
Fragments arranged by size:
2145 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3125 3125
Size: 3905
BspHI T'CATG_A
Cuts at: 3081 3081
Size: 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 955 1169 1471 1852 955
Size: 214 302 381 3008
Fragments arranged by size:
3008 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2197 2251 3735 3900 50
Size: 2147 54 1484 165 55
Fragments arranged by size:
2147 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1817 1817
Size: 3905
BsrFI r'CCGG_y
Cuts at: 1903 2084 2293 1903
Size: 181 209 3515
Fragments arranged by size:
3515 209 181
BssSI C'ACGA_G
Cuts at: 2176 2534 2176
Size: 358 3547
BstAPI GCAn_nnn'nTGC
Cuts at: 1282 1282
Size: 3905
BstBI TT'CG_AA
Cuts at: 2266 2266
Size: 3905
BstYI r'GATC_y
Cuts at: 1 930 1397 1420 1755 2001 3013 3099 3111
Size: 929 467 23 335 246 1012 86 12
Cuts at: 3111 3457 1
Size: 346 449
Fragments arranged by size:
1012 929 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 544 107
Size: 437 3468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1855 2036 2199 1855
Size: 181 163 3561
Fragments arranged by size:
3561 181 163
BtsI GCAGTG_nn'
Cuts at: 1148 1148
Size: 3905
DraI TTT'AAA
Cuts at: 904 904
Size: 3905
DrdI GACnn_nn'nnGTC
Cuts at: 645 1611 2469 645
Size: 966 858 2081
Fragments arranged by size:
2081 966 858
EaeI y'GGCC_r
Cuts at: 1490 1664 2055 2082 3427 1490
Size: 174 391 27 1345 1968
Fragments arranged by size:
1968 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1490 1490
Size: 3905
EarI CTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2113 2423 2569 3715 2113
Size: 310 146 1146 2303
Fragments arranged by size:
2303 1146 310 146
EcoICRI GAG'CTC
Cuts at: 917 917
Size: 3905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 680 1730 2162 2909 3177 196
Size: 484 1050 432 747 268 924
Fragments arranged by size:
1050 924 747 484 432 268
EcoO109I rG'GnC_Cy
Cuts at: 442 1044 442
Size: 602 3303
EcoRI G'AATT_C
Cuts at: 909 909
Size: 3905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1924 1924
Size: 3905
FspI TGC'GCA
Cuts at: 1686 1686
Size: 3905
HaeII r_GCGC'y
Cuts at: 1339 1587 2609 3588 1339
Size: 248 1022 979 1656
Fragments arranged by size:
1656 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 114 146 314 346 1747 1777 1779 1809 114
Size: 32 168 32 1401 30 2 30 2210
Fragments arranged by size:
2210 1401 168 32 32 30 30 2
HincII GTy'rAC
Cuts at: 944 944
Size: 3905
HindIII A'AGCT_T
Cuts at: 707 707
Size: 3905
KasI G'GCGC_C
Cuts at: 1335 1583 1335
Size: 248 3657
KpnI G_GTAC'C
Cuts at: 925 925
Size: 3905
MluI A'CGCG_T
Cuts at: 3338 3338
Size: 3905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 206 2576 2760 206
Size: 2370 184 1351
Fragments arranged by size:
2370 1351 184
MscI TGG'CCA
Cuts at: 1666 1666
Size: 3905
MslI CAynn'nnrTG
Cuts at: 793 1894 2021 793
Size: 1101 127 2677
Fragments arranged by size:
2677 1101 127
MspA1I CmG'CkG
Cuts at: 546 700 1016 1330 1690 2703 2948 546
Size: 154 316 314 360 1013 245 1503
Fragments arranged by size:
1503 1013 360 316 314 245 154
NaeI GCC'GGC
Cuts at: 2086 2086
Size: 3905
NarI GG'CG_CC
Cuts at: 1336 1584 1336
Size: 248 3657
NcoI C'CATG_G
Cuts at: 107 107
Size: 3905
NgoMIV G'CCGG_C
Cuts at: 2084 2084
Size: 3905
NsiI A_TGCA'T
Cuts at: 837 837
Size: 3905
NspI r_CATG'y
Cuts at: 144 958 1458 2365 144
Size: 814 500 907 1684
Fragments arranged by size:
1684 907 814 500
PciI A'CATG_T
Cuts at: 2361 2361
Size: 3905
PmeI GTTT'AAAC
Cuts at: 904 904
Size: 3905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 3873
PpuMI rG'GwC_Cy
Cuts at: 442 442
Size: 3905
PsiI TTA'TAA
Cuts at: 214 214
Size: 3905
PstI C_TGCA'G
Cuts at: 952 1637 952
Size: 685 3220
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3905
PvuII CAG'CTG
Cuts at: 700 1330 1690 700
Size: 630 360 2915
Fragments arranged by size:
2915 630 360
RsrII CG'GwC_CG
Cuts at: 2100 2100
Size: 3905
SacI G_AGCT'C
Cuts at: 919 919
Size: 3905
SacII CC_GC'GG
Cuts at: 547 547
Size: 3905
SalI G'TCGA_C
Cuts at: 942 942
Size: 3905
SapI GCTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
SbfI CC_TGCA'GG
Cuts at: 952 952
Size: 3905
ScaI AGT'ACT
Cuts at: 582 3422 582
Size: 2840 1065
SfcI C'TryA_G
Cuts at: 33 486 948 1633 2626 2817 33
Size: 453 462 685 993 191 1121
Fragments arranged by size:
1121 993 685 462 453 191
SfoI GGC'GCC
Cuts at: 1337 1585 1337
Size: 248 3657
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
SmaI CCC'GGG
Cuts at: 927 927
Size: 3905
SmlI C'TyrA_G
Cuts at: 1060 1975 2467 2729 1060
Size: 915 492 262 2236
Fragments arranged by size:
2236 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3905
SphI G_CATG'C
Cuts at: 144 958 1458 144
Size: 814 500 2591
Fragments arranged by size:
2591 814 500
StyI C'CwwG_G
Cuts at: 107 445 1039 1393 107
Size: 338 594 354 2619
Fragments arranged by size:
2619 594 354 338
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1488 1621 2080 2289 1488
Size: 133 459 209 3104
Fragments arranged by size:
3104 459 209 133
TatI w'GTAC_w
Cuts at: 580 672 3420 580
Size: 92 2748 1065
Fragments arranged by size:
2748 1065 92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 3873
Tth111I GACn'n_nGTC
Cuts at: 615 1702 615
Size: 1087 2818
XbaI T'CTAG_A
Cuts at: 258 936 258
Size: 678 3227
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1400 1400
Size: 3905
XmaI C'CCGG_G
Cuts at: 925 925
Size: 3905
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI BaeI BbsI BcgI BglI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PshAI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI