Restriction map for
pF1KB9678
(Circular) (Six-base) MAPSORT of: pF1KB9678.seq Check: 9663 from: 1 to: 3437
pF1KB9678 3437 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 365 365
Size: 3437
AccI GT'mk_AC
Cuts at: 475 475
Size: 3437
Acc65I G'GTAC_C
Cuts at: 453 453
Size: 3437
AclI AA'CG_TT
Cuts at: 3277 3277
Size: 3437
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1262 1694 2441 2709 1262
Size: 432 747 268 1990
Fragments arranged by size:
1990 747 432 268
AflIII A'CryG_T
Cuts at: 789 1893 2870 789
Size: 1104 977 1356
Fragments arranged by size:
1356 1104 977
AgeI A'CCGG_T
Cuts at: 1825 1825
Size: 3437
AlwNI CAG_nnn'CTG
Cuts at: 742 2309 742
Size: 1567 1870
ApaLI G'TGCA_C
Cuts at: 2207 2207
Size: 3437
ApoI r'AATT_y
Cuts at: 441 2790 3046 441
Size: 2349 256 832
Fragments arranged by size:
2349 832 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3437
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3437
AvaI C'yCGr_G
Cuts at: 457 457
Size: 3437
BamHI G'GATC_C
Cuts at: 462 462
Size: 3437
BanI G'GyrC_C
Cuts at: 453 867 1115 1150 453
Size: 414 248 35 2740
Fragments arranged by size:
2740 414 248 35
BanII G_rGCy'C
Cuts at: 338 451 1481 338
Size: 113 1030 2294
Fragments arranged by size:
2294 1030 113
BbeI G_GCGC'C
Cuts at: 871 1119 871
Size: 248 3189
BbvCI CC'TCA_GC
Cuts at: 181 181
Size: 3437
BciVI GTATCCnnnnn_n'
Cuts at: 1335 2096 3437 1335
Size: 761 1341 1335
Fragments arranged by size:
1341 1335 761
BclI T'GATC_A
Cuts at: 957 957
Size: 3437
BglII A'GATC_T
Cuts at: 119 952 2989 119
Size: 833 2037 567
Fragments arranged by size:
2037 833 567
BlpI GC'TnA_GC
Cuts at: 220 549 220
Size: 329 3108
Bme1580I G_kGCm'C
Cuts at: 1062 1155 2211 1062
Size: 93 1056 2288
Fragments arranged by size:
2288 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 649 730 1065 3209 649
Size: 81 335 2144 877
Fragments arranged by size:
2144 877 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1804 1804
Size: 3437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 388 191
Size: 197 3240
Bpu10I CC'TnA_GC
Cuts at: 181 317 181
Size: 136 3301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 329 406 613 1492 1984 2282 329
Size: 77 207 879 492 298 1484
Fragments arranged by size:
1484 879 492 298 207 77
BsaI GGTCTCn'nnnn_
Cuts at: 3141 3141
Size: 3437
BsaAI yAC'GTr
Cuts at: 790 790
Size: 3437
BsaBI GATnn'nnATC
Cuts at: 6 951 2988 6
Size: 945 2037 455
Fragments arranged by size:
2037 945 455
BsaHI Gr'CG_yC
Cuts at: 868 1116 1818 3314 868
Size: 248 702 1496 991
Fragments arranged by size:
1496 991 702 248
BsaWI w'CCGG_w
Cuts at: 626 1147 1825 2099 2246 2657 626
Size: 521 678 274 147 411 1406
Fragments arranged by size:
1406 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 189 219 189
Size: 30 3407
BseYI C'CCAG_C
Cuts at: 862 1620 2197 2693 862
Size: 758 577 496 1606
Fragments arranged by size:
1606 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 979 1025 2233 104
Size: 875 46 1208 1308
Fragments arranged by size:
1308 1208 875 46
BsiHKAI G_wGCw'C
Cuts at: 451 1229 1419 2211 451
Size: 778 190 792 1677
Fragments arranged by size:
1677 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 338 451 1062 1155 1229 1419 1481 2211 338
Size: 113 611 93 74 190 62 730 1564
Fragments arranged by size:
1564 730 611 190 113 93 74 62
BspEI T'CCGG_A
Cuts at: 2657 2657
Size: 3437
BspHI T'CATG_A
Cuts at: 2613 2613
Size: 3437
BspMI ACCTGCnnnn'nnnn_
Cuts at: 365 487 701 1003 1384 365
Size: 122 214 302 381 2418
Fragments arranged by size:
2418 381 302 214 122
BsrBI CCG'CTC
Cuts at: 50 141 1729 1783 3267 3432 50
Size: 91 1588 54 1484 165 55
Fragments arranged by size:
1588 1484 165 91 55 54
BsrDI GCAATG_nn'
Cuts at: 254 1349 254
Size: 1095 2342
BsrFI r'CCGG_y
Cuts at: 1435 1616 1825 1435
Size: 181 209 3047
Fragments arranged by size:
3047 209 181
BssSI C'ACGA_G
Cuts at: 1708 2066 1708
Size: 358 3079
BstAPI GCAn_nnn'nTGC
Cuts at: 294 814 294
Size: 520 2917
BstBI TT'CG_AA
Cuts at: 1798 1798
Size: 3437
BstYI r'GATC_y
Cuts at: 1 119 462 929 952 1287 1533 2545 2631
Size: 118 343 467 23 335 246 1012 86
Cuts at: 2631 2643 2989 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 343 335 246 118
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1387 1568 1731 1387
Size: 181 163 3093
Fragments arranged by size:
3093 181 163
BtsI GCAGTG_nn'
Cuts at: 680 680
Size: 3437
DraI TTT'AAA
Cuts at: 436 436
Size: 3437
DrdI GACnn_nn'nnGTC
Cuts at: 1143 2001 1143
Size: 858 2579
EaeI y'GGCC_r
Cuts at: 1022 1196 1587 1614 2959 1022
Size: 174 391 27 1345 1500
Fragments arranged by size:
1500 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1022 1022
Size: 3437
EarI CTCTTCn'nnn_
Cuts at: 343 1460 1670 343
Size: 1117 210 2110
Fragments arranged by size:
2110 1117 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1645 1955 2101 3247 1645
Size: 310 146 1146 1835
Fragments arranged by size:
1835 1146 310 146
EcoICRI GAG'CTC
Cuts at: 449 449
Size: 3437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 388 1262 1694 2441 2709 191
Size: 197 874 432 747 268 919
Fragments arranged by size:
919 874 747 432 268 197
EcoO109I rG'GnC_Cy
Cuts at: 349 576 349
Size: 227 3210
EcoRI G'AATT_C
Cuts at: 441 441
Size: 3437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1456 1456
Size: 3437
FspI TGC'GCA
Cuts at: 1218 1218
Size: 3437
HaeII r_GCGC'y
Cuts at: 170 871 1119 2141 3120 170
Size: 701 248 1022 979 487
Fragments arranged by size:
1022 979 701 487 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1279 1309 1311 1341 1279
Size: 30 2 30 3375
Fragments arranged by size:
3375 30 30 2
HincII GTy'rAC
Cuts at: 162 476 162
Size: 314 3123
KasI G'GCGC_C
Cuts at: 867 1115 867
Size: 248 3189
KpnI G_GTAC'C
Cuts at: 457 457
Size: 3437
MluI A'CGCG_T
Cuts at: 2870 2870
Size: 3437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2108 2292 2108
Size: 184 3253
MscI TGG'CCA
Cuts at: 1198 1198
Size: 3437
MslI CAynn'nnrTG
Cuts at: 1426 1553 1426
Size: 127 3310
MspA1I CmG'CkG
Cuts at: 548 862 1222 2235 2480 548
Size: 314 360 1013 245 1505
Fragments arranged by size:
1505 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1618 1618
Size: 3437
NarI GG'CG_CC
Cuts at: 868 1116 868
Size: 248 3189
NgoMIV G'CCGG_C
Cuts at: 1616 1616
Size: 3437
NspI r_CATG'y
Cuts at: 490 990 1897 490
Size: 500 907 2030
Fragments arranged by size:
2030 907 500
PciI A'CATG_T
Cuts at: 1893 1893
Size: 3437
PmeI GTTT'AAAC
Cuts at: 436 436
Size: 3437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2576 2608 2576
Size: 32 3405
PpuMI rG'GwC_Cy
Cuts at: 349 349
Size: 3437
PstI C_TGCA'G
Cuts at: 484 1169 484
Size: 685 2752
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3437
PvuII CAG'CTG
Cuts at: 862 1222 862
Size: 360 3077
RsrII CG'GwC_CG
Cuts at: 1632 1632
Size: 3437
SacI G_AGCT'C
Cuts at: 451 451
Size: 3437
SalI G'TCGA_C
Cuts at: 474 474
Size: 3437
SapI GCTCTTCn'nnn_
Cuts at: 343 1460 1670 343
Size: 1117 210 2110
Fragments arranged by size:
2110 1117 210
SbfI CC_TGCA'GG
Cuts at: 484 484
Size: 3437
ScaI AGT'ACT
Cuts at: 2954 2954
Size: 3437
SexAI A'CCwGG_T
Cuts at: 395 408 395
Size: 13 3424
SfcI C'TryA_G
Cuts at: 33 480 1165 2158 2349 33
Size: 447 685 993 191 1121
Fragments arranged by size:
1121 993 685 447 191
SfoI GGC'GCC
Cuts at: 869 1117 869
Size: 248 3189
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3437
SmaI CCC'GGG
Cuts at: 459 459
Size: 3437
SmlI C'TyrA_G
Cuts at: 344 385 592 1507 1999 2261 344
Size: 41 207 915 492 262 1520
Fragments arranged by size:
1520 915 492 262 207 41
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3437
SphI G_CATG'C
Cuts at: 490 990 490
Size: 500 2937
StuI AGG'CCT
Cuts at: 365 365
Size: 3437
StyI C'CwwG_G
Cuts at: 571 925 571
Size: 354 3083
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1020 1153 1612 1821 1020
Size: 133 459 209 2636
Fragments arranged by size:
2636 459 209 133
TatI w'GTAC_w
Cuts at: 2952 2952
Size: 3437
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 189 221 2954 2986 189
Size: 32 2733 32 640
Fragments arranged by size:
2733 640 32 32
Tth111I GACn'n_nGTC
Cuts at: 1234 1234
Size: 3437
XbaI T'CTAG_A
Cuts at: 468 468
Size: 3437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 932 932
Size: 3437
XmaI C'CCGG_G
Cuts at: 457 457
Size: 3437
XmnI GAAnn'nnTTC
Cuts at: 121 236 121
Size: 115 3322
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI