Restriction map for
pF1KB9682
(Circular) (Six-base) MAPSORT of: pF1KB9682.seq Check: 3088 from: 1 to: 3560
pF1KB9682 3560 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 598 598
Size: 3560
Acc65I G'GTAC_C
Cuts at: 576 576
Size: 3560
AclI AA'CG_TT
Cuts at: 3400 3400
Size: 3560
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 406 1385 1817 2564 2832 184
Size: 222 979 432 747 268 912
Fragments arranged by size:
979 912 747 432 268 222
AflIII A'CryG_T
Cuts at: 912 2016 2993 912
Size: 1104 977 1479
Fragments arranged by size:
1479 1104 977
AgeI A'CCGG_T
Cuts at: 1948 1948
Size: 3560
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 427 459 427
Size: 32 3528
AlwNI CAG_nnn'CTG
Cuts at: 865 2432 865
Size: 1567 1993
ApaI G_GGCC'C
Cuts at: 151 151
Size: 3560
ApaLI G'TGCA_C
Cuts at: 2330 2330
Size: 3560
ApoI r'AATT_y
Cuts at: 564 2913 3169 564
Size: 2349 256 955
Fragments arranged by size:
2349 955 256
AscI GG'CGCG_CC
Cuts at: 259 259
Size: 3560
AseI AT'TA_AT
Cuts at: 20 20
Size: 3560
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3560
AvaI C'yCGr_G
Cuts at: 460 580 460
Size: 120 3440
BamHI G'GATC_C
Cuts at: 585 585
Size: 3560
BanI G'GyrC_C
Cuts at: 250 345 576 990 1238 1273 250
Size: 95 231 414 248 35 2537
Fragments arranged by size:
2537 414 248 231 95 35
BanII G_rGCy'C
Cuts at: 151 489 574 1604 151
Size: 338 85 1030 2107
Fragments arranged by size:
2107 1030 338 85
BbeI G_GCGC'C
Cuts at: 254 994 1242 254
Size: 740 248 2572
Fragments arranged by size:
2572 740 248
BciVI GTATCCnnnnn_n'
Cuts at: 1458 2219 3560 1458
Size: 761 1341 1458
Fragments arranged by size:
1458 1341 761
BclI T'GATC_A
Cuts at: 1080 1080
Size: 3560
BglI GCCn_nnn'nGGC
Cuts at: 526 526
Size: 3560
BglII A'GATC_T
Cuts at: 1075 3112 1075
Size: 2037 1523
BlpI GC'TnA_GC
Cuts at: 493 672 493
Size: 179 3381
Bme1580I G_kGCm'C
Cuts at: 151 350 1185 1278 2334 151
Size: 199 835 93 1056 1377
Fragments arranged by size:
1377 1056 835 199 93
BmgBI CAC'GTC
Cuts at: 399 399
Size: 3560
BmrI ACTGGGnnnn_n'
Cuts at: 772 853 1188 3332 772
Size: 81 335 2144 1000
Fragments arranged by size:
2144 1000 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 453 1927 453
Size: 1474 2086
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 376
Size: 3560
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 736 1615 2107 2405 319
Size: 417 879 492 298 1474
Fragments arranged by size:
1474 879 492 417 298
BsaI GGTCTCn'nnnn_
Cuts at: 3264 3264
Size: 3560
BsaAI yAC'GTr
Cuts at: 913 913
Size: 3560
BsaBI GATnn'nnATC
Cuts at: 6 543 1074 3111 6
Size: 537 531 2037 455
Fragments arranged by size:
2037 537 531 455
BsaHI Gr'CG_yC
Cuts at: 251 991 1239 1941 3437 251
Size: 740 248 702 1496 374
Fragments arranged by size:
1496 740 702 374 248
BsaWI w'CCGG_w
Cuts at: 749 1270 1948 2222 2369 2780 749
Size: 521 678 274 147 411 1529
Fragments arranged by size:
1529 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 151 985 1743 2320 2816 151
Size: 834 758 577 496 895
Fragments arranged by size:
895 834 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 530 1102 1148 2356 104
Size: 426 572 46 1208 1308
Fragments arranged by size:
1308 1208 572 426 46
BsiHKAI G_wGCw'C
Cuts at: 574 1352 1542 2334 574
Size: 778 190 792 1800
Fragments arranged by size:
1800 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 151 350 489 574 1185 1278 1352 1542 1604
Size: 199 139 85 611 93 74 190 62
Cuts at: 1604 2334 151
Size: 730 1377
Fragments arranged by size:
1377 730 611 199 190 139 93 85
74 62
BspEI T'CCGG_A
Cuts at: 2780 2780
Size: 3560
BspHI T'CATG_A
Cuts at: 2736 2736
Size: 3560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 279 610 824 1126 1507 279
Size: 331 214 302 381 2332
Fragments arranged by size:
2332 381 331 302 214
BsrBI CCG'CTC
Cuts at: 50 185 492 1852 1906 3390 3555 50
Size: 135 307 1360 54 1484 165 55
Fragments arranged by size:
1484 1360 307 165 135 55 54
BsrDI GCAATG_nn'
Cuts at: 1472 1472
Size: 3560
BsrFI r'CCGG_y
Cuts at: 169 477 1558 1739 1948 169
Size: 308 1081 181 209 1781
Fragments arranged by size:
1781 1081 308 209 181
BssHII G'CGCG_C
Cuts at: 158 259 158
Size: 101 3459
BssSI C'ACGA_G
Cuts at: 1831 2189 1831
Size: 358 3202
BstAPI GCAn_nnn'nTGC
Cuts at: 937 937
Size: 3560
BstBI TT'CG_AA
Cuts at: 1921 1921
Size: 3560
BstYI r'GATC_y
Cuts at: 1 538 544 585 1052 1075 1410 1656 2668
Size: 537 6 41 467 23 335 246 1012
Cuts at: 2668 2754 2766 3112 1
Size: 86 12 346 449
Fragments arranged by size:
1012 537 467 449 346 335 246 86
41 23 12 6
BtgI C'CryG_G
Cuts at: 143 143
Size: 3560
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1510 1691 1854 1510
Size: 181 163 3216
Fragments arranged by size:
3216 181 163
BtsI GCAGTG_nn'
Cuts at: 803 803
Size: 3560
DraI TTT'AAA
Cuts at: 559 559
Size: 3560
DrdI GACnn_nn'nnGTC
Cuts at: 1266 2124 1266
Size: 858 2702
EaeI y'GGCC_r
Cuts at: 227 527 1145 1319 1710 1737 3082 227
Size: 300 618 174 391 27 1345 705
Fragments arranged by size:
1345 705 618 391 300 174 27
EagI C'GGCC_G
Cuts at: 527 1145 527
Size: 618 2942
EarI CTCTTCn'nnn_
Cuts at: 1583 1793 1583
Size: 210 3350
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1768 2078 2224 3370 1768
Size: 310 146 1146 1958
Fragments arranged by size:
1958 1146 310 146
EcoICRI GAG'CTC
Cuts at: 572 572
Size: 3560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 376 406 1385 1817 2564 2832 184
Size: 192 30 979 432 747 268 912
Fragments arranged by size:
979 912 747 432 268 192 30
EcoO109I rG'GnC_Cy
Cuts at: 148 417 455 699 148
Size: 269 38 244 3009
Fragments arranged by size:
3009 269 244 38
EcoRI G'AATT_C
Cuts at: 564 564
Size: 3560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1579 1579
Size: 3560
FspI TGC'GCA
Cuts at: 1341 1341
Size: 3560
HaeII r_GCGC'y
Cuts at: 254 521 994 1242 2264 3243 254
Size: 267 473 248 1022 979 571
Fragments arranged by size:
1022 979 571 473 267 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 523 555 1402 1432 1434 1464 523
Size: 32 847 30 2 30 2619
Fragments arranged by size:
2619 847 32 30 30 2
HincII GTy'rAC
Cuts at: 599 599
Size: 3560
KasI G'GCGC_C
Cuts at: 250 990 1238 250
Size: 740 248 2572
Fragments arranged by size:
2572 740 248
KpnI G_GTAC'C
Cuts at: 580 580
Size: 3560
MluI A'CGCG_T
Cuts at: 2993 2993
Size: 3560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 407 572 2231 2415 407
Size: 165 1659 184 1552
Fragments arranged by size:
1659 1552 184 165
MscI TGG'CCA
Cuts at: 229 1321 229
Size: 1092 2468
MslI CAynn'nnrTG
Cuts at: 1549 1676 1549
Size: 127 3433
MspA1I CmG'CkG
Cuts at: 145 155 671 985 1345 2358 2603 145
Size: 10 516 314 360 1013 245 1102
Fragments arranged by size:
1102 1013 516 360 314 245 10
NaeI GCC'GGC
Cuts at: 171 1741 171
Size: 1570 1990
NarI GG'CG_CC
Cuts at: 251 991 1239 251
Size: 740 248 2572
Fragments arranged by size:
2572 740 248
NgoMIV G'CCGG_C
Cuts at: 169 1739 169
Size: 1570 1990
NspI r_CATG'y
Cuts at: 613 1113 2020 613
Size: 500 907 2153
Fragments arranged by size:
2153 907 500
PciI A'CATG_T
Cuts at: 2016 2016
Size: 3560
PmeI GTTT'AAAC
Cuts at: 559 559
Size: 3560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2699 2731 2699
Size: 32 3528
PpuMI rG'GwC_Cy
Cuts at: 417 455 417
Size: 38 3522
PspOMI G'GGCC_C
Cuts at: 147 147
Size: 3560
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 307 339 307
Size: 32 3528
PstI C_TGCA'G
Cuts at: 607 1292 607
Size: 685 2875
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3560
PvuII CAG'CTG
Cuts at: 155 985 1345 155
Size: 830 360 2370
Fragments arranged by size:
2370 830 360
RsrII CG'GwC_CG
Cuts at: 480 1755 480
Size: 1275 2285
SacI G_AGCT'C
Cuts at: 574 574
Size: 3560
SacII CC_GC'GG
Cuts at: 146 146
Size: 3560
SalI G'TCGA_C
Cuts at: 597 597
Size: 3560
SanDI GG'GwC_CC
Cuts at: 455 455
Size: 3560
SapI GCTCTTCn'nnn_
Cuts at: 1583 1793 1583
Size: 210 3350
SbfI CC_TGCA'GG
Cuts at: 607 607
Size: 3560
ScaI AGT'ACT
Cuts at: 3077 3077
Size: 3560
SfcI C'TryA_G
Cuts at: 33 603 1288 2281 2472 33
Size: 570 685 993 191 1121
Fragments arranged by size:
1121 993 685 570 191
SfoI GGC'GCC
Cuts at: 252 992 1240 252
Size: 740 248 2572
Fragments arranged by size:
2572 740 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3560
SmaI CCC'GGG
Cuts at: 462 582 462
Size: 120 3440
SmlI C'TyrA_G
Cuts at: 334 715 1630 2122 2384 334
Size: 381 915 492 262 1510
Fragments arranged by size:
1510 915 492 381 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3560
SphI G_CATG'C
Cuts at: 613 1113 613
Size: 500 3060
StyI C'CwwG_G
Cuts at: 694 1048 694
Size: 354 3206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1143 1276 1735 1944 1143
Size: 133 459 209 2759
Fragments arranged by size:
2759 459 209 133
TatI w'GTAC_w
Cuts at: 3075 3075
Size: 3560
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3077 3109 3077
Size: 32 3528
Tth111I GACn'n_nGTC
Cuts at: 1357 1357
Size: 3560
XbaI T'CTAG_A
Cuts at: 591 591
Size: 3560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1055 1055
Size: 3560
XmaI C'CCGG_G
Cuts at: 460 580 460
Size: 120 3440
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspXI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI