Restriction map for
pF1KB9684
(Circular) (Six-base) MAPSORT of: pF1KB9684.seq Check: 8641 from: 1 to: 3605
pF1KB9684 3605 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 643 643
Size: 3605
Acc65I G'GTAC_C
Cuts at: 621 621
Size: 3605
AclI AA'CG_TT
Cuts at: 3445 3445
Size: 3605
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 304 1430 1862 2609 2877 189
Size: 115 1126 432 747 268 917
Fragments arranged by size:
1126 917 747 432 268 115
AfeI AGC'GCT
Cuts at: 202 202
Size: 3605
AflIII A'CryG_T
Cuts at: 957 2061 3038 957
Size: 1104 977 1524
Fragments arranged by size:
1524 1104 977
AgeI A'CCGG_T
Cuts at: 1993 1993
Size: 3605
AlwNI CAG_nnn'CTG
Cuts at: 910 2477 910
Size: 1567 2038
ApaLI G'TGCA_C
Cuts at: 2375 2375
Size: 3605
ApoI r'AATT_y
Cuts at: 609 2958 3214 609
Size: 2349 256 1000
Fragments arranged by size:
2349 1000 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3605
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3605
AvaI C'yCGr_G
Cuts at: 625 625
Size: 3605
BamHI G'GATC_C
Cuts at: 630 630
Size: 3605
BanI G'GyrC_C
Cuts at: 621 1035 1283 1318 621
Size: 414 248 35 2908
Fragments arranged by size:
2908 414 248 35
BanII G_rGCy'C
Cuts at: 619 1649 619
Size: 1030 2575
BbeI G_GCGC'C
Cuts at: 1039 1287 1039
Size: 248 3357
BbsI GAAGACnn'nnnn_
Cuts at: 431 431
Size: 3605
BbvCI CC'TCA_GC
Cuts at: 509 509
Size: 3605
BciVI GTATCCnnnnn_n'
Cuts at: 1503 2264 3605 1503
Size: 761 1341 1503
Fragments arranged by size:
1503 1341 761
BclI T'GATC_A
Cuts at: 1125 1125
Size: 3605
BglII A'GATC_T
Cuts at: 1120 3157 1120
Size: 2037 1568
BlpI GC'TnA_GC
Cuts at: 717 717
Size: 3605
Bme1580I G_kGCm'C
Cuts at: 1230 1323 2379 1230
Size: 93 1056 2456
Fragments arranged by size:
2456 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 817 898 1233 3377 817
Size: 81 335 2144 1045
Fragments arranged by size:
2144 1045 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1972 1972
Size: 3605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 555 337
Size: 218 3387
Bpu10I CC'TnA_GC
Cuts at: 509 509
Size: 3605
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 545 781 1660 2152 2450 463
Size: 82 236 879 492 298 1618
Fragments arranged by size:
1618 879 492 298 236 82
BsaI GGTCTCn'nnnn_
Cuts at: 361 3309 361
Size: 2948 657
BsaAI yAC'GTr
Cuts at: 958 958
Size: 3605
BsaBI GATnn'nnATC
Cuts at: 6 1119 3156 6
Size: 1113 2037 455
Fragments arranged by size:
2037 1113 455
BsaHI Gr'CG_yC
Cuts at: 1036 1284 1986 3482 1036
Size: 248 702 1496 1159
Fragments arranged by size:
1496 1159 702 248
BsaWI w'CCGG_w
Cuts at: 794 1315 1993 2267 2414 2825 794
Size: 521 678 274 147 411 1574
Fragments arranged by size:
1574 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 232 517 554 1030 1788 2365 2861 232
Size: 285 37 476 758 577 496 976
Fragments arranged by size:
976 758 577 496 476 285 37
BsiEI CG_ry'CG
Cuts at: 104 1147 1193 2401 104
Size: 1043 46 1208 1308
Fragments arranged by size:
1308 1208 1043 46
BsiHKAI G_wGCw'C
Cuts at: 386 619 1397 1587 2379 386
Size: 233 778 190 792 1612
Fragments arranged by size:
1612 792 778 233 190
Bsp1286I G_dGCh'C
Cuts at: 386 619 1230 1323 1397 1587 1649 2379 386
Size: 233 611 93 74 190 62 730 1612
Fragments arranged by size:
1612 730 611 233 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2825 2825
Size: 3605
BspHI T'CATG_A
Cuts at: 240 2781 240
Size: 2541 1064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 450 655 869 1171 1552 450
Size: 205 214 302 381 2503
Fragments arranged by size:
2503 381 302 214 205
BsrBI CCG'CTC
Cuts at: 50 1897 1951 3435 3600 50
Size: 1847 54 1484 165 55
Fragments arranged by size:
1847 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1517 1517
Size: 3605
BsrFI r'CCGG_y
Cuts at: 1603 1784 1993 1603
Size: 181 209 3215
Fragments arranged by size:
3215 209 181
BssSI C'ACGA_G
Cuts at: 1876 2234 1876
Size: 358 3247
BstAPI GCAn_nnn'nTGC
Cuts at: 982 982
Size: 3605
BstBI TT'CG_AA
Cuts at: 1966 1966
Size: 3605
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3605
BstYI r'GATC_y
Cuts at: 1 479 630 1097 1120 1455 1701 2713 2799
Size: 478 151 467 23 335 246 1012 86
Cuts at: 2799 2811 3157 1
Size: 12 346 449
Fragments arranged by size:
1012 478 467 449 346 335 246 151
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3605
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1555 1736 1899 1555
Size: 181 163 3261
Fragments arranged by size:
3261 181 163
BtsI GCAGTG_nn'
Cuts at: 384 848 384
Size: 464 3141
DraI TTT'AAA
Cuts at: 604 604
Size: 3605
DrdI GACnn_nn'nnGTC
Cuts at: 1311 2169 1311
Size: 858 2747
EaeI y'GGCC_r
Cuts at: 1190 1364 1755 1782 3127 1190
Size: 174 391 27 1345 1668
Fragments arranged by size:
1668 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1190 1190
Size: 3605
EarI CTCTTCn'nnn_
Cuts at: 586 598 1628 1838 586
Size: 12 1030 210 2353
Fragments arranged by size:
2353 1030 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1813 2123 2269 3415 1813
Size: 310 146 1146 2003
Fragments arranged by size:
2003 1146 310 146
EcoICRI GAG'CTC
Cuts at: 617 617
Size: 3605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 304 337 555 1430 1862 2609 2877 189
Size: 115 33 218 875 432 747 268 917
Fragments arranged by size:
917 875 747 432 268 218 115 33
EcoNI CCTnn'n_nnAGG
Cuts at: 375 375
Size: 3605
EcoO109I rG'GnC_Cy
Cuts at: 744 744
Size: 3605
EcoRI G'AATT_C
Cuts at: 609 609
Size: 3605
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1624 1624
Size: 3605
FspI TGC'GCA
Cuts at: 1386 1386
Size: 3605
HaeII r_GCGC'y
Cuts at: 204 1039 1287 2309 3288 204
Size: 835 248 1022 979 521
Fragments arranged by size:
1022 979 835 521 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1447 1477 1479 1509 1447
Size: 30 2 30 3543
Fragments arranged by size:
3543 30 30 2
HincII GTy'rAC
Cuts at: 644 644
Size: 3605
KasI G'GCGC_C
Cuts at: 1035 1283 1035
Size: 248 3357
KpnI G_GTAC'C
Cuts at: 625 625
Size: 3605
MluI A'CGCG_T
Cuts at: 3038 3038
Size: 3605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 311 2276 2460 311
Size: 1965 184 1456
Fragments arranged by size:
1965 1456 184
MscI TGG'CCA
Cuts at: 1366 1366
Size: 3605
MslI CAynn'nnrTG
Cuts at: 112 1594 1721 112
Size: 1482 127 1996
Fragments arranged by size:
1996 1482 127
MspA1I CmG'CkG
Cuts at: 232 517 716 1030 1390 2403 2648 232
Size: 285 199 314 360 1013 245 1189
Fragments arranged by size:
1189 1013 360 314 285 245 199
NaeI GCC'GGC
Cuts at: 1786 1786
Size: 3605
NarI GG'CG_CC
Cuts at: 1036 1284 1036
Size: 248 3357
NcoI C'CATG_G
Cuts at: 107 107
Size: 3605
NgoMIV G'CCGG_C
Cuts at: 1784 1784
Size: 3605
NspI r_CATG'y
Cuts at: 658 1158 2065 658
Size: 500 907 2198
Fragments arranged by size:
2198 907 500
PasI CC'CwG_GG
Cuts at: 120 120
Size: 3605
PciI A'CATG_T
Cuts at: 2061 2061
Size: 3605
PfoI T'CCnGG_A
Cuts at: 314 314
Size: 3605
PmeI GTTT'AAAC
Cuts at: 604 604
Size: 3605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2744 2776 2744
Size: 32 3573
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 431 463 431
Size: 32 3573
PstI C_TGCA'G
Cuts at: 652 1337 652
Size: 685 2920
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3605
PvuII CAG'CTG
Cuts at: 232 517 1030 1390 232
Size: 285 513 360 2447
Fragments arranged by size:
2447 513 360 285
RsrII CG'GwC_CG
Cuts at: 1800 1800
Size: 3605
SacI G_AGCT'C
Cuts at: 619 619
Size: 3605
SalI G'TCGA_C
Cuts at: 642 642
Size: 3605
SapI GCTCTTCn'nnn_
Cuts at: 1628 1838 1628
Size: 210 3395
SbfI CC_TGCA'GG
Cuts at: 652 652
Size: 3605
ScaI AGT'ACT
Cuts at: 3122 3122
Size: 3605
SfcI C'TryA_G
Cuts at: 33 648 1333 2326 2517 33
Size: 615 685 993 191 1121
Fragments arranged by size:
1121 993 685 615 191
SfoI GGC'GCC
Cuts at: 1037 1285 1037
Size: 248 3357
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3605
SmaI CCC'GGG
Cuts at: 627 627
Size: 3605
SmlI C'TyrA_G
Cuts at: 442 560 760 1675 2167 2429 442
Size: 118 200 915 492 262 1618
Fragments arranged by size:
1618 915 492 262 200 118
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3605
SphI G_CATG'C
Cuts at: 658 1158 658
Size: 500 3105
StyI C'CwwG_G
Cuts at: 107 739 1093 107
Size: 632 354 2619
Fragments arranged by size:
2619 632 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 363 1188 1321 1780 1989 363
Size: 825 133 459 209 1979
Fragments arranged by size:
1979 825 459 209 133
TatI w'GTAC_w
Cuts at: 218 3120 218
Size: 2902 703
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3122 3154 3122
Size: 32 3573
Tth111I GACn'n_nGTC
Cuts at: 1402 1402
Size: 3605
XbaI T'CTAG_A
Cuts at: 636 636
Size: 3605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1100 1100
Size: 3605
XmaI C'CCGG_G
Cuts at: 625 625
Size: 3605
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI