Restriction map for
pF1KB9685
(Circular) (Six-base) MAPSORT of: pF1KB9685.seq Check: 2989 from: 1 to: 3698
pF1KB9685 3698 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 736 736
Size: 3698
Acc65I G'GTAC_C
Cuts at: 714 714
Size: 3698
AclI AA'CG_TT
Cuts at: 3538 3538
Size: 3698
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1523 1955 2702 2970 1523
Size: 432 747 268 2251
Fragments arranged by size:
2251 747 432 268
AflIII A'CryG_T
Cuts at: 1050 2154 3131 1050
Size: 1104 977 1617
Fragments arranged by size:
1617 1104 977
AgeI A'CCGG_T
Cuts at: 2086 2086
Size: 3698
AhdI GACnn_n'nnGTC
Cuts at: 226 226
Size: 3698
AlwNI CAG_nnn'CTG
Cuts at: 1003 2570 1003
Size: 1567 2131
ApaLI G'TGCA_C
Cuts at: 2468 2468
Size: 3698
ApoI r'AATT_y
Cuts at: 121 142 186 582 686 702 3051 3307 121
Size: 21 44 396 104 16 2349 256 512
Fragments arranged by size:
2349 512 396 256 104 44 21 16
AseI AT'TA_AT
Cuts at: 20 20
Size: 3698
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
AvaI C'yCGr_G
Cuts at: 718 718
Size: 3698
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 542 575 542
Size: 33 3665
BamHI G'GATC_C
Cuts at: 723 723
Size: 3698
BanI G'GyrC_C
Cuts at: 714 1128 1376 1411 714
Size: 414 248 35 3001
Fragments arranged by size:
3001 414 248 35
BanII G_rGCy'C
Cuts at: 712 1742 712
Size: 1030 2668
BbeI G_GCGC'C
Cuts at: 1132 1380 1132
Size: 248 3450
BbsI GAAGACnn'nnnn_
Cuts at: 222 222
Size: 3698
BciVI GTATCCnnnnn_n'
Cuts at: 1596 2357 3698 1596
Size: 761 1341 1596
Fragments arranged by size:
1596 1341 761
BclI T'GATC_A
Cuts at: 263 1218 263
Size: 955 2743
BglII A'GATC_T
Cuts at: 1213 3250 1213
Size: 2037 1661
BlpI GC'TnA_GC
Cuts at: 810 810
Size: 3698
Bme1580I G_kGCm'C
Cuts at: 1323 1416 2472 1323
Size: 93 1056 2549
Fragments arranged by size:
2549 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 325 910 991 1326 3470 325
Size: 585 81 335 2144 553
Fragments arranged by size:
2144 585 553 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 3698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 295 172
Size: 123 3575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1753 2245 2543 874
Size: 879 492 298 2029
Fragments arranged by size:
2029 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3402 3402
Size: 3698
BsaAI yAC'GTr
Cuts at: 1051 1051
Size: 3698
BsaBI GATnn'nnATC
Cuts at: 6 1212 3249 6
Size: 1206 2037 455
Fragments arranged by size:
2037 1206 455
BsaHI Gr'CG_yC
Cuts at: 1129 1377 2079 3575 1129
Size: 248 702 1496 1252
Fragments arranged by size:
1496 1252 702 248
BsaWI w'CCGG_w
Cuts at: 887 1408 2086 2360 2507 2918 887
Size: 521 678 274 147 411 1667
Fragments arranged by size:
1667 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 169
Size: 3698
BseYI C'CCAG_C
Cuts at: 1123 1881 2458 2954 1123
Size: 758 577 496 1867
Fragments arranged by size:
1867 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 161
Size: 3698
BsiEI CG_ry'CG
Cuts at: 104 1240 1286 2494 104
Size: 1136 46 1208 1308
Fragments arranged by size:
1308 1208 1136 46
BsiHKAI G_wGCw'C
Cuts at: 712 1490 1680 2472 712
Size: 778 190 792 1938
Fragments arranged by size:
1938 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 712 1323 1416 1490 1680 1742 2472 712
Size: 611 93 74 190 62 730 1938
Fragments arranged by size:
1938 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2918 2918
Size: 3698
BspHI T'CATG_A
Cuts at: 2874 2874
Size: 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 748 962 1264 1645 748
Size: 214 302 381 2801
Fragments arranged by size:
2801 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1990 2044 3528 3693 50
Size: 1940 54 1484 165 55
Fragments arranged by size:
1940 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 162 1610 162
Size: 1448 2250
BsrFI r'CCGG_y
Cuts at: 1696 1877 2086 1696
Size: 181 209 3308
Fragments arranged by size:
3308 209 181
BssSI C'ACGA_G
Cuts at: 1969 2327 1969
Size: 358 3340
BstAPI GCAn_nnn'nTGC
Cuts at: 1075 1075
Size: 3698
BstBI TT'CG_AA
Cuts at: 2059 2059
Size: 3698
BstYI r'GATC_y
Cuts at: 1 723 1190 1213 1548 1794 2806 2892 2904
Size: 722 467 23 335 246 1012 86 12
Cuts at: 2904 3250 1
Size: 346 449
Fragments arranged by size:
1012 722 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3698
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1648 1829 1992 1648
Size: 181 163 3354
Fragments arranged by size:
3354 181 163
BtsI GCAGTG_nn'
Cuts at: 173 941 173
Size: 768 2930
DraI TTT'AAA
Cuts at: 697 697
Size: 3698
DrdI GACnn_nn'nnGTC
Cuts at: 1404 2262 1404
Size: 858 2840
EaeI y'GGCC_r
Cuts at: 134 1283 1457 1848 1875 3220 134
Size: 1149 174 391 27 1345 612
Fragments arranged by size:
1345 1149 612 391 174 27
EagI C'GGCC_G
Cuts at: 1283 1283
Size: 3698
EarI CTCTTCn'nnn_
Cuts at: 298 1721 1931 298
Size: 1423 210 2065
Fragments arranged by size:
2065 1423 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1906 2216 2362 3508 1906
Size: 310 146 1146 2096
Fragments arranged by size:
2096 1146 310 146
EcoICRI GAG'CTC
Cuts at: 710 710
Size: 3698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 295 1523 1955 2702 2970 172
Size: 123 1228 432 747 268 900
Fragments arranged by size:
1228 900 747 432 268 123
EcoO109I rG'GnC_Cy
Cuts at: 837 837
Size: 3698
EcoRI G'AATT_C
Cuts at: 142 702 142
Size: 560 3138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1717 1717
Size: 3698
FspI TGC'GCA
Cuts at: 1479 1479
Size: 3698
HaeII r_GCGC'y
Cuts at: 1132 1380 2402 3381 1132
Size: 248 1022 979 1449
Fragments arranged by size:
1449 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 212 244 255 287 1540 1570 1572 1602 212
Size: 32 11 32 1253 30 2 30 2308
Fragments arranged by size:
2308 1253 32 32 30 30 11 2
HincII GTy'rAC
Cuts at: 737 737
Size: 3698
HindIII A'AGCT_T
Cuts at: 647 647
Size: 3698
KasI G'GCGC_C
Cuts at: 1128 1376 1128
Size: 248 3450
KpnI G_GTAC'C
Cuts at: 718 718
Size: 3698
MluI A'CGCG_T
Cuts at: 3131 3131
Size: 3698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2369 2553 2369
Size: 184 3514
MscI TGG'CCA
Cuts at: 136 1459 136
Size: 1323 2375
MslI CAynn'nnrTG
Cuts at: 1687 1814 1687
Size: 127 3571
MspA1I CmG'CkG
Cuts at: 809 1123 1483 2496 2741 809
Size: 314 360 1013 245 1766
Fragments arranged by size:
1766 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1879 1879
Size: 3698
NarI GG'CG_CC
Cuts at: 1129 1377 1129
Size: 248 3450
NcoI C'CATG_G
Cuts at: 107 107
Size: 3698
NdeI CA'TA_TG
Cuts at: 555 555
Size: 3698
NgoMIV G'CCGG_C
Cuts at: 1877 1877
Size: 3698
NspI r_CATG'y
Cuts at: 751 1251 2158 751
Size: 500 907 2291
Fragments arranged by size:
2291 907 500
PciI A'CATG_T
Cuts at: 2154 2154
Size: 3698
PmeI GTTT'AAAC
Cuts at: 697 697
Size: 3698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 3666
PshAI GACnn'nnGTC
Cuts at: 362 362
Size: 3698
PsiI TTA'TAA
Cuts at: 497 497
Size: 3698
PstI C_TGCA'G
Cuts at: 745 1430 745
Size: 685 3013
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3698
PvuII CAG'CTG
Cuts at: 1123 1483 1123
Size: 360 3338
RsrII CG'GwC_CG
Cuts at: 1893 1893
Size: 3698
SacI G_AGCT'C
Cuts at: 712 712
Size: 3698
SalI G'TCGA_C
Cuts at: 735 735
Size: 3698
SapI GCTCTTCn'nnn_
Cuts at: 1721 1931 1721
Size: 210 3488
SbfI CC_TGCA'GG
Cuts at: 745 745
Size: 3698
ScaI AGT'ACT
Cuts at: 3215 3215
Size: 3698
SexAI A'CCwGG_T
Cuts at: 224 224
Size: 3698
SfcI C'TryA_G
Cuts at: 33 216 741 1426 2419 2610 33
Size: 183 525 685 993 191 1121
Fragments arranged by size:
1121 993 685 525 191 183
SfoI GGC'GCC
Cuts at: 1130 1378 1130
Size: 248 3450
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
SmaI CCC'GGG
Cuts at: 720 720
Size: 3698
SmlI C'TyrA_G
Cuts at: 853 1768 2260 2522 853
Size: 915 492 262 2029
Fragments arranged by size:
2029 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3698
SphI G_CATG'C
Cuts at: 751 1251 751
Size: 500 3198
StyI C'CwwG_G
Cuts at: 107 234 832 1186 107
Size: 127 598 354 2619
Fragments arranged by size:
2619 598 354 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1281 1414 1873 2082 1281
Size: 133 459 209 2897
Fragments arranged by size:
2897 459 209 133
TatI w'GTAC_w
Cuts at: 480 3213 480
Size: 2733 965
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 3666
Tth111I GACn'n_nGTC
Cuts at: 1495 1495
Size: 3698
XbaI T'CTAG_A
Cuts at: 729 729
Size: 3698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1193 114
Size: 1079 2619
XmaI C'CCGG_G
Cuts at: 718 718
Size: 3698
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PmeI PpiI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI