Restriction map for
pF1KB9689
(Circular) (Six-base) MAPSORT of: pF1KB9689.seq Check: 6684 from: 1 to: 3803
pF1KB9689 3803 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 841 841
Size: 3803
Acc65I G'GTAC_C
Cuts at: 819 819
Size: 3803
AclI AA'CG_TT
Cuts at: 3643 3643
Size: 3803
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1628 2060 2807 3075 1628
Size: 432 747 268 2356
Fragments arranged by size:
2356 747 432 268
AflIII A'CryG_T
Cuts at: 1155 2259 3236 1155
Size: 1104 977 1722
Fragments arranged by size:
1722 1104 977
AgeI A'CCGG_T
Cuts at: 2191 2191
Size: 3803
AlwNI CAG_nnn'CTG
Cuts at: 1108 2675 1108
Size: 1567 2236
ApaLI G'TGCA_C
Cuts at: 613 2573 613
Size: 1960 1843
ApoI r'AATT_y
Cuts at: 807 3156 3412 807
Size: 2349 256 1198
Fragments arranged by size:
2349 1198 256
AscI GG'CGCG_CC
Cuts at: 686 686
Size: 3803
AseI AT'TA_AT
Cuts at: 20 20
Size: 3803
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3803
AvaI C'yCGr_G
Cuts at: 116 236 823 116
Size: 120 587 3096
Fragments arranged by size:
3096 587 120
BamHI G'GATC_C
Cuts at: 828 828
Size: 3803
BanI G'GyrC_C
Cuts at: 176 693 747 819 1233 1481 1516 176
Size: 517 54 72 414 248 35 2463
Fragments arranged by size:
2463 517 414 248 72 54 35
BanII G_rGCy'C
Cuts at: 329 817 1847 329
Size: 488 1030 2285
Fragments arranged by size:
2285 1030 488
BbeI G_GCGC'C
Cuts at: 180 697 751 1237 1485 180
Size: 517 54 486 248 2498
Fragments arranged by size:
2498 517 486 248 54
BbvCI CC'TCA_GC
Cuts at: 211 211
Size: 3803
BciVI GTATCCnnnnn_n'
Cuts at: 1701 2462 3803 1701
Size: 761 1341 1701
Fragments arranged by size:
1701 1341 761
BclI T'GATC_A
Cuts at: 1323 1323
Size: 3803
BglI GCCn_nnn'nGGC
Cuts at: 340 457 577 340
Size: 117 120 3566
Fragments arranged by size:
3566 120 117
BglII A'GATC_T
Cuts at: 1318 3355 1318
Size: 2037 1766
BlpI GC'TnA_GC
Cuts at: 915 915
Size: 3803
Bme1580I G_kGCm'C
Cuts at: 617 1428 1521 2577 617
Size: 811 93 1056 1843
Fragments arranged by size:
1843 1056 811 93
BmrI ACTGGGnnnn_n'
Cuts at: 358 604 1015 1096 1431 3575 358
Size: 246 411 81 335 2144 586
Fragments arranged by size:
2144 586 411 335 246 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 3803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 286 329 527 804 247
Size: 39 43 198 277 3246
Fragments arranged by size:
3246 277 198 43 39
Bpu10I CC'TnA_GC
Cuts at: 211 211
Size: 3803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 324 979 1858 2350 2648 139
Size: 185 655 879 492 298 1294
Fragments arranged by size:
1294 879 655 492 298 185
BsaI GGTCTCn'nnnn_
Cuts at: 780 3507 780
Size: 2727 1076
BsaAI yAC'GTr
Cuts at: 1156 1156
Size: 3803
BsaBI GATnn'nnATC
Cuts at: 6 1317 3354 6
Size: 1311 2037 455
Fragments arranged by size:
2037 1311 455
BsaHI Gr'CG_yC
Cuts at: 177 374 449 694 748 1234 1482 2184 3680
Size: 197 75 245 54 486 248 702 1496
Cuts at: 3680 177
Size: 300
Fragments arranged by size:
1496 702 486 300 248 245 197 75
54
BsaWI w'CCGG_w
Cuts at: 251 403 703 992 1513 2191 2465 2612 3023
Size: 152 300 289 521 678 274 147 411
Cuts at: 3023 251
Size: 1031
Fragments arranged by size:
1031 678 521 411 300 289 274 152
147
BseYI C'CCAG_C
Cuts at: 470 1228 1986 2563 3059 470
Size: 758 758 577 496 1214
Fragments arranged by size:
1214 758 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 580 472
Size: 108 3695
BsiEI CG_ry'CG
Cuts at: 104 335 461 1345 1391 2599 104
Size: 231 126 884 46 1208 1308
Fragments arranged by size:
1308 1208 884 231 126 46
BsiHKAI G_wGCw'C
Cuts at: 617 817 1595 1785 2577 617
Size: 200 778 190 792 1843
Fragments arranged by size:
1843 792 778 200 190
Bsp1286I G_dGCh'C
Cuts at: 329 617 817 1428 1521 1595 1785 1847 2577
Size: 288 200 611 93 74 190 62 730
Cuts at: 2577 329
Size: 1555
Fragments arranged by size:
1555 730 611 288 200 190 93 74
62
BspEI T'CCGG_A
Cuts at: 251 3023 251
Size: 2772 1031
BspHI T'CATG_A
Cuts at: 2979 2979
Size: 3803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 853 1067 1369 1750 853
Size: 214 302 381 2906
Fragments arranged by size:
2906 381 302 214
BsrBI CCG'CTC
Cuts at: 50 231 2095 2149 3633 3798 50
Size: 181 1864 54 1484 165 55
Fragments arranged by size:
1864 1484 181 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1715 1715
Size: 3803
BsrFI r'CCGG_y
Cuts at: 525 576 690 1801 1982 2191 525
Size: 51 114 1111 181 209 2137
Fragments arranged by size:
2137 1111 209 181 114 51
BssHII G'CGCG_C
Cuts at: 669 686 669
Size: 17 3786
BssSI C'ACGA_G
Cuts at: 2074 2432 2074
Size: 358 3445
BstAPI GCAn_nnn'nTGC
Cuts at: 1180 1180
Size: 3803
BstBI TT'CG_AA
Cuts at: 2164 2164
Size: 3803
BstEII G'GTnAC_C
Cuts at: 111 111
Size: 3803
BstYI r'GATC_y
Cuts at: 1 531 828 1295 1318 1653 1899 2911 2997
Size: 530 297 467 23 335 246 1012 86
Cuts at: 2997 3009 3355 1
Size: 12 346 449
Fragments arranged by size:
1012 530 467 449 346 335 297 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 293 338 107
Size: 186 45 3572
Fragments arranged by size:
3572 186 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1753 1934 2097 1753
Size: 181 163 3459
Fragments arranged by size:
3459 181 163
BtsI GCAGTG_nn'
Cuts at: 1046 1046
Size: 3803
DraI TTT'AAA
Cuts at: 802 802
Size: 3803
DrdI GACnn_nn'nnGTC
Cuts at: 1509 2367 1509
Size: 858 2945
EaeI y'GGCC_r
Cuts at: 332 458 1388 1562 1953 1980 3325 332
Size: 126 930 174 391 27 1345 810
Fragments arranged by size:
1345 930 810 391 174 126 27
EagI C'GGCC_G
Cuts at: 332 458 1388 332
Size: 126 930 2747
Fragments arranged by size:
2747 930 126
EarI CTCTTCn'nnn_
Cuts at: 200 481 1826 2036 200
Size: 281 1345 210 1967
Fragments arranged by size:
1967 1345 281 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2011 2321 2467 3613 2011
Size: 310 146 1146 2201
Fragments arranged by size:
2201 1146 310 146
EcoICRI GAG'CTC
Cuts at: 815 815
Size: 3803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 286 329 527 804 1628 2060 2807 3075
Size: 39 43 198 277 824 432 747 268
Cuts at: 3075 247
Size: 975
Fragments arranged by size:
975 824 747 432 277 268 198 43
39
EcoO109I rG'GnC_Cy
Cuts at: 756 942 756
Size: 186 3617
EcoRI G'AATT_C
Cuts at: 807 807
Size: 3803
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1822 1822
Size: 3803
FspI TGC'GCA
Cuts at: 642 1584 642
Size: 942 2861
HaeII r_GCGC'y
Cuts at: 180 385 550 606 697 751 1237 1485 2507
Size: 205 165 56 91 54 486 248 1022
Cuts at: 2507 3486 180
Size: 979 497
Fragments arranged by size:
1022 979 497 486 248 205 165 91
56 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1645 1675 1677 1707 1645
Size: 30 2 30 3741
Fragments arranged by size:
3741 30 30 2
HincII GTy'rAC
Cuts at: 842 842
Size: 3803
KasI G'GCGC_C
Cuts at: 176 693 747 1233 1481 176
Size: 517 54 486 248 2498
Fragments arranged by size:
2498 517 486 248 54
KpnI G_GTAC'C
Cuts at: 823 823
Size: 3803
MluI A'CGCG_T
Cuts at: 3236 3236
Size: 3803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2474 2658 2474
Size: 184 3619
MscI TGG'CCA
Cuts at: 1564 1564
Size: 3803
MslI CAynn'nnrTG
Cuts at: 1792 1919 1792
Size: 127 3676
MspA1I CmG'CkG
Cuts at: 340 392 762 914 1228 1588 2601 2846 340
Size: 52 370 152 314 360 1013 245 1297
Fragments arranged by size:
1297 1013 370 360 314 245 152 52
NaeI GCC'GGC
Cuts at: 692 1984 692
Size: 1292 2511
NarI GG'CG_CC
Cuts at: 177 694 748 1234 1482 177
Size: 517 54 486 248 2498
Fragments arranged by size:
2498 517 486 248 54
NcoI C'CATG_G
Cuts at: 107 107
Size: 3803
NgoMIV G'CCGG_C
Cuts at: 690 1982 690
Size: 1292 2511
NspI r_CATG'y
Cuts at: 856 1356 2263 856
Size: 500 907 2396
Fragments arranged by size:
2396 907 500
PciI A'CATG_T
Cuts at: 2259 2259
Size: 3803
PfoI T'CCnGG_A
Cuts at: 368 368
Size: 3803
PmeI GTTT'AAAC
Cuts at: 802 802
Size: 3803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2942 2974 2942
Size: 32 3771
PstI C_TGCA'G
Cuts at: 850 1535 850
Size: 685 3118
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3803
PvuII CAG'CTG
Cuts at: 392 1228 1588 392
Size: 836 360 2607
Fragments arranged by size:
2607 836 360
RsrII CG'GwC_CG
Cuts at: 1998 1998
Size: 3803
SacI G_AGCT'C
Cuts at: 817 817
Size: 3803
SacII CC_GC'GG
Cuts at: 341 341
Size: 3803
SalI G'TCGA_C
Cuts at: 840 840
Size: 3803
SapI GCTCTTCn'nnn_
Cuts at: 200 481 1826 2036 200
Size: 281 1345 210 1967
Fragments arranged by size:
1967 1345 281 210
SbfI CC_TGCA'GG
Cuts at: 850 850
Size: 3803
ScaI AGT'ACT
Cuts at: 3320 3320
Size: 3803
SfcI C'TryA_G
Cuts at: 33 846 1531 2524 2715 33
Size: 813 685 993 191 1121
Fragments arranged by size:
1121 993 813 685 191
SfoI GGC'GCC
Cuts at: 178 695 749 1235 1483 178
Size: 517 54 486 248 2498
Fragments arranged by size:
2498 517 486 248 54
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3803
SgrAI Cr'CCGG_yG
Cuts at: 690 690
Size: 3803
SmaI CCC'GGG
Cuts at: 118 238 825 118
Size: 120 587 3096
Fragments arranged by size:
3096 587 120
SmlI C'TyrA_G
Cuts at: 154 303 958 1873 2365 2627 154
Size: 149 655 915 492 262 1330
Fragments arranged by size:
1330 915 655 492 262 149
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3803
SphI G_CATG'C
Cuts at: 856 1356 856
Size: 500 3303
StuI AGG'CCT
Cuts at: 540 540
Size: 3803
StyI C'CwwG_G
Cuts at: 107 582 752 790 937 1291 107
Size: 475 170 38 147 354 2619
Fragments arranged by size:
2619 475 354 170 147 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1386 1519 1978 2187 1386
Size: 133 459 209 3002
Fragments arranged by size:
3002 459 209 133
TatI w'GTAC_w
Cuts at: 3318 3318
Size: 3803
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3320 3352 3320
Size: 32 3771
Tth111I GACn'n_nGTC
Cuts at: 1600 1600
Size: 3803
XbaI T'CTAG_A
Cuts at: 834 834
Size: 3803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1298 1298
Size: 3803
XmaI C'CCGG_G
Cuts at: 116 236 823 116
Size: 120 587 3096
Fragments arranged by size:
3096 587 120
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI