Restriction map for
pF1KB9691
(Circular) (Six-base) MAPSORT of: pF1KB9691.seq Check: 9486 from: 1 to: 3803
pF1KB9691 3803 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 841 841
Size: 3803
Acc65I G'GTAC_C
Cuts at: 819 819
Size: 3803
AclI AA'CG_TT
Cuts at: 3643 3643
Size: 3803
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 467 1628 2060 2807 3075 467
Size: 1161 432 747 268 1195
Fragments arranged by size:
1195 1161 747 432 268
AflIII A'CryG_T
Cuts at: 1155 2259 3236 1155
Size: 1104 977 1722
Fragments arranged by size:
1722 1104 977
AgeI A'CCGG_T
Cuts at: 2191 2191
Size: 3803
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 535 567 535
Size: 32 3771
AlwNI CAG_nnn'CTG
Cuts at: 490 534 1108 2675 490
Size: 44 574 1567 1618
Fragments arranged by size:
1618 1567 574 44
ApaLI G'TGCA_C
Cuts at: 2573 2573
Size: 3803
ApoI r'AATT_y
Cuts at: 807 3156 3412 807
Size: 2349 256 1198
Fragments arranged by size:
2349 1198 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3803
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3803
AvaI C'yCGr_G
Cuts at: 376 706 823 376
Size: 330 117 3356
Fragments arranged by size:
3356 330 117
BamHI G'GATC_C
Cuts at: 828 828
Size: 3803
BanI G'GyrC_C
Cuts at: 235 241 325 403 578 712 819 1233 1481
Size: 6 84 78 175 134 107 414 248
Cuts at: 1481 1516 235
Size: 35 2522
Fragments arranged by size:
2522 414 248 175 134 107 84 78
35 6
BanII G_rGCy'C
Cuts at: 301 383 817 1847 301
Size: 82 434 1030 2257
Fragments arranged by size:
2257 1030 434 82
BbeI G_GCGC'C
Cuts at: 239 245 329 407 582 716 1237 1485 239
Size: 6 84 78 175 134 521 248 2557
Fragments arranged by size:
2557 521 248 175 134 84 78 6
BbsI GAAGACnn'nnnn_
Cuts at: 473 791 473
Size: 318 3485
BbvCI CC'TCA_GC
Cuts at: 446 446
Size: 3803
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 375 409 576 610 375
Size: 34 167 34 3568
Fragments arranged by size:
3568 167 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1701 2462 3803 1701
Size: 761 1341 1701
Fragments arranged by size:
1701 1341 761
BclI T'GATC_A
Cuts at: 1323 1323
Size: 3803
BglII A'GATC_T
Cuts at: 632 1318 3355 632
Size: 686 2037 1080
Fragments arranged by size:
2037 1080 686
BlpI GC'TnA_GC
Cuts at: 915 915
Size: 3803
Bme1580I G_kGCm'C
Cuts at: 722 1428 1521 2577 722
Size: 706 93 1056 1948
Fragments arranged by size:
1948 1056 706 93
BmrI ACTGGGnnnn_n'
Cuts at: 1015 1096 1431 3575 1015
Size: 81 335 2144 1243
Fragments arranged by size:
2144 1243 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 3803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 562 556
Size: 6 3797
Bpu10I CC'TnA_GC
Cuts at: 446 446
Size: 3803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 1858 2350 2648 979
Size: 879 492 298 2134
Fragments arranged by size:
2134 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3507 3507
Size: 3803
BsaAI yAC'GTr
Cuts at: 1156 1156
Size: 3803
BsaBI GATnn'nnATC
Cuts at: 6 631 1317 3354 6
Size: 625 686 2037 455
Fragments arranged by size:
2037 686 625 455
BsaHI Gr'CG_yC
Cuts at: 236 242 326 332 404 579 713 1234 1482
Size: 6 84 6 72 175 134 521 248
Cuts at: 1482 2184 3680 236
Size: 702 1496 359
Fragments arranged by size:
1496 702 521 359 248 175 134 84
72 6 6
BsaWI w'CCGG_w
Cuts at: 992 1513 2191 2465 2612 3023 992
Size: 521 678 274 147 411 1772
Fragments arranged by size:
1772 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 535 565 535
Size: 30 3773
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 775 805 775
Size: 30 3773
BseYI C'CCAG_C
Cuts at: 1228 1986 2563 3059 1228
Size: 758 577 496 1972
Fragments arranged by size:
1972 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 257 1345 1391 2599 104
Size: 153 1088 46 1208 1308
Fragments arranged by size:
1308 1208 1088 153 46
BsiHKAI G_wGCw'C
Cuts at: 817 1595 1785 2577 817
Size: 778 190 792 2043
Fragments arranged by size:
2043 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 301 383 722 817 1428 1521 1595 1785 1847
Size: 82 339 95 611 93 74 190 62
Cuts at: 1847 2577 301
Size: 730 1527
Fragments arranged by size:
1527 730 611 339 190 95 93 82
74 62
BspEI T'CCGG_A
Cuts at: 3023 3023
Size: 3803
BspHI T'CATG_A
Cuts at: 2979 2979
Size: 3803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 353 853 1067 1369 1750 353
Size: 500 214 302 381 2406
Fragments arranged by size:
2406 500 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2095 2149 3633 3798 50
Size: 2045 54 1484 165 55
Fragments arranged by size:
2045 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1715 1715
Size: 3803
BsrFI r'CCGG_y
Cuts at: 238 1801 1982 2191 238
Size: 1563 181 209 1850
Fragments arranged by size:
1850 1563 209 181
BssHII G'CGCG_C
Cuts at: 428 428
Size: 3803
BssSI C'ACGA_G
Cuts at: 2074 2432 2074
Size: 358 3445
BstAPI GCAn_nnn'nTGC
Cuts at: 1180 1180
Size: 3803
BstBI TT'CG_AA
Cuts at: 2164 2164
Size: 3803
BstYI r'GATC_y
Cuts at: 1 632 828 1295 1318 1653 1899 2911 2997
Size: 631 196 467 23 335 246 1012 86
Cuts at: 2997 3009 3355 1
Size: 12 346 449
Fragments arranged by size:
1012 631 467 449 346 335 246 196
86 23 12
BtgI C'CryG_G
Cuts at: 743 743
Size: 3803
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1753 1934 2097 1753
Size: 181 163 3459
Fragments arranged by size:
3459 181 163
BtsI GCAGTG_nn'
Cuts at: 393 734 1046 393
Size: 341 312 3150
Fragments arranged by size:
3150 341 312
DraI TTT'AAA
Cuts at: 802 802
Size: 3803
DrdI GACnn_nn'nnGTC
Cuts at: 1509 2367 1509
Size: 858 2945
EaeI y'GGCC_r
Cuts at: 1388 1562 1953 1980 3325 1388
Size: 174 391 27 1345 1866
Fragments arranged by size:
1866 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1388 1388
Size: 3803
EarI CTCTTCn'nnn_
Cuts at: 773 1826 2036 773
Size: 1053 210 2540
Fragments arranged by size:
2540 1053 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2011 2321 2467 3613 2011
Size: 310 146 1146 2201
Fragments arranged by size:
2201 1146 310 146
EcoICRI GAG'CTC
Cuts at: 815 815
Size: 3803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 467 556 562 1628 2060 2807 3075 467
Size: 89 6 1066 432 747 268 1195
Fragments arranged by size:
1195 1066 747 432 268 89 6
EcoO109I rG'GnC_Cy
Cuts at: 487 942 487
Size: 455 3348
EcoRI G'AATT_C
Cuts at: 807 807
Size: 3803
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1822 1822
Size: 3803
FspI TGC'GCA
Cuts at: 1584 1584
Size: 3803
HaeII r_GCGC'y
Cuts at: 239 245 329 407 582 624 716 1237 1485
Size: 6 84 78 175 42 92 521 248
Cuts at: 1485 2507 3486 239
Size: 1022 979 556
Fragments arranged by size:
1022 979 556 521 248 175 92 84
78 42 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1645 1675 1677 1707 1645
Size: 30 2 30 3741
Fragments arranged by size:
3741 30 30 2
HincII GTy'rAC
Cuts at: 279 842 279
Size: 563 3240
KasI G'GCGC_C
Cuts at: 235 241 325 403 578 712 1233 1481 235
Size: 6 84 78 175 134 521 248 2557
Fragments arranged by size:
2557 521 248 175 134 84 78 6
KpnI G_GTAC'C
Cuts at: 823 823
Size: 3803
MluI A'CGCG_T
Cuts at: 3236 3236
Size: 3803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 712 2474 2658 712
Size: 1762 184 1857
Fragments arranged by size:
1857 1762 184
MscI TGG'CCA
Cuts at: 1564 1564
Size: 3803
MslI CAynn'nnrTG
Cuts at: 1792 1919 1792
Size: 127 3676
MspA1I CmG'CkG
Cuts at: 745 914 1228 1588 2601 2846 745
Size: 169 314 360 1013 245 1702
Fragments arranged by size:
1702 1013 360 314 245 169
NaeI GCC'GGC
Cuts at: 240 1984 240
Size: 1744 2059
NarI GG'CG_CC
Cuts at: 236 242 326 404 579 713 1234 1482 236
Size: 6 84 78 175 134 521 248 2557
Fragments arranged by size:
2557 521 248 175 134 84 78 6
NgoMIV G'CCGG_C
Cuts at: 238 1982 238
Size: 1744 2059
NspI r_CATG'y
Cuts at: 856 1356 2263 856
Size: 500 907 2396
Fragments arranged by size:
2396 907 500
PciI A'CATG_T
Cuts at: 2259 2259
Size: 3803
PflMI CCAn_nnn'nTGG
Cuts at: 534 534
Size: 3803
PmeI GTTT'AAAC
Cuts at: 802 802
Size: 3803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 535 567 2942 2974 535
Size: 32 2375 32 1364
Fragments arranged by size:
2375 1364 32 32
PpuMI rG'GwC_Cy
Cuts at: 487 487
Size: 3803
PshAI GACnn'nnGTC
Cuts at: 683 683
Size: 3803
PstI C_TGCA'G
Cuts at: 388 850 1535 388
Size: 462 685 2656
Fragments arranged by size:
2656 685 462
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3803
PvuII CAG'CTG
Cuts at: 1228 1588 1228
Size: 360 3443
RsrII CG'GwC_CG
Cuts at: 1998 1998
Size: 3803
SacI G_AGCT'C
Cuts at: 817 817
Size: 3803
SacII CC_GC'GG
Cuts at: 746 746
Size: 3803
SalI G'TCGA_C
Cuts at: 840 840
Size: 3803
SapI GCTCTTCn'nnn_
Cuts at: 1826 2036 1826
Size: 210 3593
SbfI CC_TGCA'GG
Cuts at: 850 850
Size: 3803
ScaI AGT'ACT
Cuts at: 3320 3320
Size: 3803
SfcI C'TryA_G
Cuts at: 33 384 846 1531 2524 2715 33
Size: 351 462 685 993 191 1121
Fragments arranged by size:
1121 993 685 462 351 191
SfoI GGC'GCC
Cuts at: 237 243 327 405 580 714 1235 1483 237
Size: 6 84 78 175 134 521 248 2557
Fragments arranged by size:
2557 521 248 175 134 84 78 6
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3803
SgrAI Cr'CCGG_yG
Cuts at: 238 238
Size: 3803
SmaI CCC'GGG
Cuts at: 378 825 378
Size: 447 3356
SmlI C'TyrA_G
Cuts at: 958 1873 2365 2627 958
Size: 915 492 262 2134
Fragments arranged by size:
2134 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3803
SphI G_CATG'C
Cuts at: 856 1356 856
Size: 500 3303
StyI C'CwwG_G
Cuts at: 937 1291 937
Size: 354 3449
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1386 1519 1978 2187 1386
Size: 133 459 209 3002
Fragments arranged by size:
3002 459 209 133
TatI w'GTAC_w
Cuts at: 3318 3318
Size: 3803
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3320 3352 3320
Size: 32 3771
Tth111I GACn'n_nGTC
Cuts at: 1600 1600
Size: 3803
XbaI T'CTAG_A
Cuts at: 834 834
Size: 3803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1298 1298
Size: 3803
XmaI C'CCGG_G
Cuts at: 376 823 376
Size: 447 3356
XmnI GAAnn'nnTTC
Cuts at: 550 550
Size: 3803
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
XhoI ZraI