Restriction map for
pF1KB9692
(Circular) (Six-base) MAPSORT of: pF1KB9692.seq Check: 6409 from: 1 to: 3842
pF1KB9692 3842 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 880 880
Size: 3842
Acc65I G'GTAC_C
Cuts at: 858 858
Size: 3842
AclI AA'CG_TT
Cuts at: 3682 3682
Size: 3842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1667 2099 2846 3114 1667
Size: 432 747 268 2395
Fragments arranged by size:
2395 747 432 268
AflIII A'CryG_T
Cuts at: 1194 2298 3275 1194
Size: 1104 977 1761
Fragments arranged by size:
1761 1104 977
AgeI A'CCGG_T
Cuts at: 2230 2230
Size: 3842
AleI CACnn'nnGTG
Cuts at: 220 220
Size: 3842
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 583 615 583
Size: 32 3810
AlwNI CAG_nnn'CTG
Cuts at: 201 582 780 1147 2714 201
Size: 381 198 367 1567 1329
Fragments arranged by size:
1567 1329 381 367 198
ApaI G_GGCC'C
Cuts at: 792 792
Size: 3842
ApaLI G'TGCA_C
Cuts at: 2612 2612
Size: 3842
ApoI r'AATT_y
Cuts at: 846 3195 3451 846
Size: 2349 256 1237
Fragments arranged by size:
2349 1237 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3842
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
AvaI C'yCGr_G
Cuts at: 862 862
Size: 3842
BamHI G'GATC_C
Cuts at: 408 867 408
Size: 459 3383
BanI G'GyrC_C
Cuts at: 858 1272 1520 1555 858
Size: 414 248 35 3145
Fragments arranged by size:
3145 414 248 35
BanII G_rGCy'C
Cuts at: 115 481 493 792 856 1886 115
Size: 366 12 299 64 1030 2071
Fragments arranged by size:
2071 1030 366 299 64 12
BbeI G_GCGC'C
Cuts at: 1276 1524 1276
Size: 248 3594
BciVI GTATCCnnnnn_n'
Cuts at: 1740 2501 3842 1740
Size: 761 1341 1740
Fragments arranged by size:
1740 1341 761
BclI T'GATC_A
Cuts at: 1362 1362
Size: 3842
BglI GCCn_nnn'nGGC
Cuts at: 796 796
Size: 3842
BglII A'GATC_T
Cuts at: 1357 3394 1357
Size: 2037 1805
BlpI GC'TnA_GC
Cuts at: 954 954
Size: 3842
Bme1580I G_kGCm'C
Cuts at: 792 1467 1560 2616 792
Size: 675 93 1056 2018
Fragments arranged by size:
2018 1056 675 93
BmrI ACTGGGnnnn_n'
Cuts at: 1054 1135 1470 3614 1054
Size: 81 335 2144 1282
Fragments arranged by size:
2144 1282 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 3842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 610 787 802 604
Size: 6 177 15 3644
Fragments arranged by size:
3644 177 15 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1897 2389 2687 1018
Size: 879 492 298 2173
Fragments arranged by size:
2173 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3546 3546
Size: 3842
BsaAI yAC'GTr
Cuts at: 1195 1195
Size: 3842
BsaBI GATnn'nnATC
Cuts at: 6 1356 3393 6
Size: 1350 2037 455
Fragments arranged by size:
2037 1350 455
BsaHI Gr'CG_yC
Cuts at: 1273 1521 2223 3719 1273
Size: 248 702 1496 1396
Fragments arranged by size:
1496 1396 702 248
BsaWI w'CCGG_w
Cuts at: 695 1031 1552 2230 2504 2651 3062 695
Size: 336 521 678 274 147 411 1475
Fragments arranged by size:
1475 678 521 411 336 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 583 613 583
Size: 30 3812
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 481 775 481
Size: 294 3548
BseYI C'CCAG_C
Cuts at: 232 739 1267 2025 2602 3098 232
Size: 507 528 758 577 496 976
Fragments arranged by size:
976 758 577 528 507 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 442
Size: 3842
BsiEI CG_ry'CG
Cuts at: 104 210 1384 1430 2638 104
Size: 106 1174 46 1208 1308
Fragments arranged by size:
1308 1208 1174 106 46
BsiHKAI G_wGCw'C
Cuts at: 115 856 1634 1824 2616 115
Size: 741 778 190 792 1341
Fragments arranged by size:
1341 792 778 741 190
Bsp1286I G_dGCh'C
Cuts at: 115 481 493 792 856 1467 1560 1634 1824
Size: 366 12 299 64 611 93 74 190
Cuts at: 1824 1886 2616 115
Size: 62 730 1341
Fragments arranged by size:
1341 730 611 366 299 190 93 74
64 62 12
BspEI T'CCGG_A
Cuts at: 3062 3062
Size: 3842
BspHI T'CATG_A
Cuts at: 3018 3018
Size: 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 892 1106 1408 1789 892
Size: 214 302 381 2945
Fragments arranged by size:
2945 381 302 214
BsrBI CCG'CTC
Cuts at: 50 786 2134 2188 3672 3837 50
Size: 736 1348 54 1484 165 55
Fragments arranged by size:
1484 1348 736 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1754 1754
Size: 3842
BsrFI r'CCGG_y
Cuts at: 1840 2021 2230 1840
Size: 181 209 3452
Fragments arranged by size:
3452 209 181
BssSI C'ACGA_G
Cuts at: 2113 2471 2113
Size: 358 3484
BstAPI GCAn_nnn'nTGC
Cuts at: 749 1219 749
Size: 470 3372
BstBI TT'CG_AA
Cuts at: 2203 2203
Size: 3842
BstXI CCAn_nnnn'nTGG
Cuts at: 459 459
Size: 3842
BstYI r'GATC_y
Cuts at: 1 408 867 1334 1357 1692 1938 2950 3036
Size: 407 459 467 23 335 246 1012 86
Cuts at: 3036 3048 3394 1
Size: 12 346 449
Fragments arranged by size:
1012 467 459 449 407 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 218 282 302 332 342 218
Size: 64 20 30 10 3718
Fragments arranged by size:
3718 64 30 20 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1792 1973 2136 1792
Size: 181 163 3498
Fragments arranged by size:
3498 181 163
BtsI GCAGTG_nn'
Cuts at: 1085 1085
Size: 3842
DraI TTT'AAA
Cuts at: 841 841
Size: 3842
DrdI GACnn_nn'nnGTC
Cuts at: 1548 2406 1548
Size: 858 2984
EaeI y'GGCC_r
Cuts at: 207 1427 1601 1992 2019 3364 207
Size: 1220 174 391 27 1345 685
Fragments arranged by size:
1345 1220 685 391 174 27
EagI C'GGCC_G
Cuts at: 207 1427 207
Size: 1220 2622
EarI CTCTTCn'nnn_
Cuts at: 514 1865 2075 514
Size: 1351 210 2281
Fragments arranged by size:
2281 1351 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 817 2050 2360 2506 3652 817
Size: 1233 310 146 1146 1007
Fragments arranged by size:
1233 1146 1007 310 146
EcoICRI GAG'CTC
Cuts at: 113 854 113
Size: 741 3101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 610 787 802 1667 2099 2846 3114 604
Size: 6 177 15 865 432 747 268 1332
Fragments arranged by size:
1332 865 747 432 268 177 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 463 463
Size: 3842
EcoO109I rG'GnC_Cy
Cuts at: 198 348 789 981 198
Size: 150 441 192 3059
Fragments arranged by size:
3059 441 192 150
EcoRI G'AATT_C
Cuts at: 846 846
Size: 3842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3842
FspI TGC'GCA
Cuts at: 404 1623 404
Size: 1219 2623
HaeII r_GCGC'y
Cuts at: 1276 1524 2546 3525 1276
Size: 248 1022 979 1593
Fragments arranged by size:
1593 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1684 1714 1716 1746 1684
Size: 30 2 30 3780
Fragments arranged by size:
3780 30 30 2
HincII GTy'rAC
Cuts at: 126 881 126
Size: 755 3087
HindIII A'AGCT_T
Cuts at: 439 439
Size: 3842
KasI G'GCGC_C
Cuts at: 1272 1520 1272
Size: 248 3594
KpnI G_GTAC'C
Cuts at: 862 862
Size: 3842
MluI A'CGCG_T
Cuts at: 3275 3275
Size: 3842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2513 2697 2513
Size: 184 3658
MscI TGG'CCA
Cuts at: 1603 1603
Size: 3842
MslI CAynn'nnrTG
Cuts at: 220 1831 1958 220
Size: 1611 127 2104
Fragments arranged by size:
2104 1611 127
MspA1I CmG'CkG
Cuts at: 239 378 455 953 1267 1627 2640 2885 239
Size: 139 77 498 314 360 1013 245 1196
Fragments arranged by size:
1196 1013 498 360 314 245 139 77
NaeI GCC'GGC
Cuts at: 2023 2023
Size: 3842
NarI GG'CG_CC
Cuts at: 1273 1521 1273
Size: 248 3594
NgoMIV G'CCGG_C
Cuts at: 2021 2021
Size: 3842
NspI r_CATG'y
Cuts at: 895 1395 2302 895
Size: 500 907 2435
Fragments arranged by size:
2435 907 500
PasI CC'CwG_GG
Cuts at: 654 654
Size: 3842
PciI A'CATG_T
Cuts at: 2298 2298
Size: 3842
PflMI CCAn_nnn'nTGG
Cuts at: 201 582 201
Size: 381 3461
PfoI T'CCnGG_A
Cuts at: 269 269
Size: 3842
PmeI GTTT'AAAC
Cuts at: 841 841
Size: 3842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 583 615 2981 3013 583
Size: 32 2366 32 1412
Fragments arranged by size:
2366 1412 32 32
PpuMI rG'GwC_Cy
Cuts at: 198 348 198
Size: 150 3692
PspOMI G'GGCC_C
Cuts at: 788 788
Size: 3842
PstI C_TGCA'G
Cuts at: 889 1574 889
Size: 685 3157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3842
PvuII CAG'CTG
Cuts at: 1267 1627 1267
Size: 360 3482
RsrII CG'GwC_CG
Cuts at: 2037 2037
Size: 3842
SacI G_AGCT'C
Cuts at: 115 856 115
Size: 741 3101
SalI G'TCGA_C
Cuts at: 879 879
Size: 3842
SanDI GG'GwC_CC
Cuts at: 348 348
Size: 3842
SapI GCTCTTCn'nnn_
Cuts at: 514 1865 2075 514
Size: 1351 210 2281
Fragments arranged by size:
2281 1351 210
SbfI CC_TGCA'GG
Cuts at: 889 889
Size: 3842
ScaI AGT'ACT
Cuts at: 3359 3359
Size: 3842
SexAI A'CCwGG_T
Cuts at: 416 416
Size: 3842
SfcI C'TryA_G
Cuts at: 33 564 885 1570 2563 2754 33
Size: 531 321 685 993 191 1121
Fragments arranged by size:
1121 993 685 531 321 191
SfoI GGC'GCC
Cuts at: 1274 1522 1274
Size: 248 3594
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
SmaI CCC'GGG
Cuts at: 864 864
Size: 3842
SmlI C'TyrA_G
Cuts at: 997 1912 2404 2666 997
Size: 915 492 262 2173
Fragments arranged by size:
2173 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3842
SphI G_CATG'C
Cuts at: 895 1395 895
Size: 500 3342
StyI C'CwwG_G
Cuts at: 976 1330 976
Size: 354 3488
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1425 1558 2017 2226 1425
Size: 133 459 209 3041
Fragments arranged by size:
3041 459 209 133
TatI w'GTAC_w
Cuts at: 3357 3357
Size: 3842
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 494 526 3359 3391 494
Size: 32 2833 32 945
Fragments arranged by size:
2833 945 32 32
Tth111I GACn'n_nGTC
Cuts at: 1639 1639
Size: 3842
XbaI T'CTAG_A
Cuts at: 873 873
Size: 3842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1337 1337
Size: 3842
XmaI C'CCGG_G
Cuts at: 862 862
Size: 3842
XmnI GAAnn'nnTTC
Cuts at: 598 598
Size: 3842
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SacII SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI