Restriction map for
pF1KB9694
(Circular) (Six-base) MAPSORT of: pF1KB9694.seq Check: 639 from: 1 to: 3869
pF1KB9694 3869 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 296 907 296
Size: 611 3258
Acc65I G'GTAC_C
Cuts at: 885 885
Size: 3869
AclI AA'CG_TT
Cuts at: 3709 3709
Size: 3869
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1694 2126 2873 3141 1694
Size: 432 747 268 2422
Fragments arranged by size:
2422 747 432 268
AfeI AGC'GCT
Cuts at: 130 130
Size: 3869
AflIII A'CryG_T
Cuts at: 1221 2325 3302 1221
Size: 1104 977 1788
Fragments arranged by size:
1788 1104 977
AgeI A'CCGG_T
Cuts at: 803 2257 803
Size: 1454 2415
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 301 333 301
Size: 32 3837
AlwNI CAG_nnn'CTG
Cuts at: 747 1174 2741 747
Size: 427 1567 1875
Fragments arranged by size:
1875 1567 427
ApaLI G'TGCA_C
Cuts at: 361 2639 361
Size: 2278 1591
ApoI r'AATT_y
Cuts at: 873 3222 3478 873
Size: 2349 256 1264
Fragments arranged by size:
2349 1264 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3869
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
AvaI C'yCGr_G
Cuts at: 529 889 529
Size: 360 3509
BamHI G'GATC_C
Cuts at: 450 575 894 450
Size: 125 319 3425
Fragments arranged by size:
3425 319 125
BanI G'GyrC_C
Cuts at: 120 369 649 885 1299 1547 1582 120
Size: 249 280 236 414 248 35 2407
Fragments arranged by size:
2407 414 280 249 248 236 35
BanII G_rGCy'C
Cuts at: 883 1913 883
Size: 1030 2839
BbeI G_GCGC'C
Cuts at: 124 653 1303 1551 124
Size: 529 650 248 2442
Fragments arranged by size:
2442 650 529 248
BbvCI CC'TCA_GC
Cuts at: 679 679
Size: 3869
BciVI GTATCCnnnnn_n'
Cuts at: 1767 2528 3869 1767
Size: 761 1341 1767
Fragments arranged by size:
1767 1341 761
BclI T'GATC_A
Cuts at: 1389 1389
Size: 3869
BglII A'GATC_T
Cuts at: 1384 3421 1384
Size: 2037 1832
BlpI GC'TnA_GC
Cuts at: 981 981
Size: 3869
Bme1580I G_kGCm'C
Cuts at: 365 1494 1587 2643 365
Size: 1129 93 1056 1591
Fragments arranged by size:
1591 1129 1056 93
BmgBI CAC'GTC
Cuts at: 849 849
Size: 3869
BmrI ACTGGGnnnn_n'
Cuts at: 1081 1162 1497 3641 1081
Size: 81 335 2144 1309
Fragments arranged by size:
2144 1309 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 348 2236 348
Size: 1888 1981
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 769 213
Size: 556 3313
Bpu10I CC'TnA_GC
Cuts at: 679 679
Size: 3869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1924 2416 2714 1045
Size: 879 492 298 2200
Fragments arranged by size:
2200 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 336 3573 336
Size: 3237 632
BsaAI yAC'GTr
Cuts at: 642 1222 642
Size: 580 3289
BsaBI GATnn'nnATC
Cuts at: 6 1383 3420 6
Size: 1377 2037 455
Fragments arranged by size:
2037 1377 455
BsaHI Gr'CG_yC
Cuts at: 121 293 650 1300 1548 2250 3746 121
Size: 172 357 650 248 702 1496 244
Fragments arranged by size:
1496 702 650 357 248 244 172
BsaWI w'CCGG_w
Cuts at: 803 834 1058 1579 2257 2531 2678 3089 803
Size: 31 224 521 678 274 147 411 1583
Fragments arranged by size:
1583 678 521 411 274 224 147 31
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 211 241 211
Size: 30 3839
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 399 399
Size: 3869
BseYI C'CCAG_C
Cuts at: 599 1294 2052 2629 3125 599
Size: 695 758 577 496 1343
Fragments arranged by size:
1343 758 695 577 496
BsiEI CG_ry'CG
Cuts at: 104 269 1411 1457 2665 104
Size: 165 1142 46 1208 1308
Fragments arranged by size:
1308 1208 1142 165 46
BsiHKAI G_wGCw'C
Cuts at: 365 883 1661 1851 2643 365
Size: 518 778 190 792 1591
Fragments arranged by size:
1591 792 778 518 190
Bsp1286I G_dGCh'C
Cuts at: 365 883 1494 1587 1661 1851 1913 2643 365
Size: 518 611 93 74 190 62 730 1591
Fragments arranged by size:
1591 730 611 518 190 93 74 62
BspEI T'CCGG_A
Cuts at: 834 3089 834
Size: 2255 1614
BspHI T'CATG_A
Cuts at: 3045 3045
Size: 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 919 1133 1435 1816 919
Size: 214 302 381 2972
Fragments arranged by size:
2972 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2161 2215 3699 3864 50
Size: 2111 54 1484 165 55
Fragments arranged by size:
2111 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1781 1781
Size: 3869
BsrFI r'CCGG_y
Cuts at: 117 283 803 1867 2048 2257 117
Size: 166 520 1064 181 209 1729
Fragments arranged by size:
1729 1064 520 209 181 166
BssSI C'ACGA_G
Cuts at: 2140 2498 2140
Size: 358 3511
BstAPI GCAn_nnn'nTGC
Cuts at: 369 1246 369
Size: 877 2992
BstBI TT'CG_AA
Cuts at: 2230 2230
Size: 3869
BstYI r'GATC_y
Cuts at: 1 450 575 894 1361 1384 1719 1965 2977
Size: 449 125 319 467 23 335 246 1012
Cuts at: 2977 3063 3075 3421 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 449 346 335 319 246
125 86 23 12
BtgI C'CryG_G
Cuts at: 206 206
Size: 3869
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1819 2000 2163 487
Size: 1332 181 163 2193
Fragments arranged by size:
2193 1332 181 163
BtsI GCAGTG_nn'
Cuts at: 1112 1112
Size: 3869
DraI TTT'AAA
Cuts at: 868 868
Size: 3869
DraIII CAC_nnn'GTG
Cuts at: 369 642 369
Size: 273 3596
DrdI GACnn_nn'nnGTC
Cuts at: 1575 2433 1575
Size: 858 3011
EaeI y'GGCC_r
Cuts at: 155 266 389 1454 1628 2019 2046 3391 155
Size: 111 123 1065 174 391 27 1345 633
Fragments arranged by size:
1345 1065 633 391 174 123 111 27
EagI C'GGCC_G
Cuts at: 266 1454 266
Size: 1188 2681
EarI CTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3659
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2077 2387 2533 3679 2077
Size: 310 146 1146 2267
Fragments arranged by size:
2267 1146 310 146
EcoICRI GAG'CTC
Cuts at: 881 881
Size: 3869
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 769 1694 2126 2873 3141 213
Size: 556 925 432 747 268 941
Fragments arranged by size:
941 925 747 556 432 268
EcoO109I rG'GnC_Cy
Cuts at: 192 1008 192
Size: 816 3053
EcoRI G'AATT_C
Cuts at: 873 873
Size: 3869
EcoRV GAT'ATC
Cuts at: 493 493
Size: 3869
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 150 1888 150
Size: 1738 2131
FspI TGC'GCA
Cuts at: 1650 1650
Size: 3869
HaeII r_GCGC'y
Cuts at: 124 132 653 1303 1551 2573 3552 124
Size: 8 521 650 248 1022 979 441
Fragments arranged by size:
1022 979 650 521 441 248 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 838 870 1711 1741 1743 1773 838
Size: 32 841 30 2 30 2934
Fragments arranged by size:
2934 841 32 30 30 2
HincII GTy'rAC
Cuts at: 908 908
Size: 3869
KasI G'GCGC_C
Cuts at: 120 649 1299 1547 120
Size: 529 650 248 2442
Fragments arranged by size:
2442 650 529 248
KpnI G_GTAC'C
Cuts at: 889 889
Size: 3869
MluI A'CGCG_T
Cuts at: 3302 3302
Size: 3869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 326 2540 2724 326
Size: 2214 184 1471
Fragments arranged by size:
2214 1471 184
MscI TGG'CCA
Cuts at: 157 1630 157
Size: 1473 2396
MslI CAynn'nnrTG
Cuts at: 1858 1985 1858
Size: 127 3742
MspA1I CmG'CkG
Cuts at: 208 447 666 980 1294 1654 2667 2912 208
Size: 239 219 314 314 360 1013 245 1165
Fragments arranged by size:
1165 1013 360 314 314 245 239 219
NaeI GCC'GGC
Cuts at: 119 2050 119
Size: 1931 1938
NarI GG'CG_CC
Cuts at: 121 650 1300 1548 121
Size: 529 650 248 2442
Fragments arranged by size:
2442 650 529 248
NgoMIV G'CCGG_C
Cuts at: 117 2048 117
Size: 1931 1938
NspI r_CATG'y
Cuts at: 922 1422 2329 922
Size: 500 907 2462
Fragments arranged by size:
2462 907 500
PciI A'CATG_T
Cuts at: 2325 2325
Size: 3869
PmeI GTTT'AAAC
Cuts at: 868 868
Size: 3869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 3837
PpuMI rG'GwC_Cy
Cuts at: 192 192
Size: 3869
PstI C_TGCA'G
Cuts at: 916 1601 916
Size: 685 3184
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3869
PvuII CAG'CTG
Cuts at: 666 1294 1654 666
Size: 628 360 2881
Fragments arranged by size:
2881 628 360
RsrII CG'GwC_CG
Cuts at: 2064 2064
Size: 3869
SacI G_AGCT'C
Cuts at: 883 883
Size: 3869
SacII CC_GC'GG
Cuts at: 209 209
Size: 3869
SalI G'TCGA_C
Cuts at: 906 906
Size: 3869
SapI GCTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3659
SbfI CC_TGCA'GG
Cuts at: 916 916
Size: 3869
ScaI AGT'ACT
Cuts at: 3386 3386
Size: 3869
SfcI C'TryA_G
Cuts at: 33 912 1597 2590 2781 33
Size: 879 685 993 191 1121
Fragments arranged by size:
1121 993 879 685 191
SfoI GGC'GCC
Cuts at: 122 651 1301 1549 122
Size: 529 650 248 2442
Fragments arranged by size:
2442 650 529 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
SmaI CCC'GGG
Cuts at: 891 891
Size: 3869
SmlI C'TyrA_G
Cuts at: 1024 1939 2431 2693 1024
Size: 915 492 262 2200
Fragments arranged by size:
2200 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3869
SphI G_CATG'C
Cuts at: 922 1422 922
Size: 500 3369
StyI C'CwwG_G
Cuts at: 158 510 1003 1357 158
Size: 352 493 354 2670
Fragments arranged by size:
2670 493 354 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1452 1585 2044 2253 1452
Size: 133 459 209 3068
Fragments arranged by size:
3068 459 209 133
TatI w'GTAC_w
Cuts at: 3384 3384
Size: 3869
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3386 3418 3386
Size: 32 3837
Tth111I GACn'n_nGTC
Cuts at: 1666 1666
Size: 3869
XbaI T'CTAG_A
Cuts at: 900 900
Size: 3869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 147 309 1364 147
Size: 162 1055 2652
Fragments arranged by size:
2652 1055 162
XmaI C'CCGG_G
Cuts at: 889 889
Size: 3869
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI