Restriction map for
pF1KB9696
(Circular) (Six-base) MAPSORT of: pF1KB9696.seq Check: 6334 from: 1 to: 3908
pF1KB9696 3908 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 687 687
Size: 3908
AccI GT'mk_AC
Cuts at: 587 946 587
Size: 359 3549
Acc65I G'GTAC_C
Cuts at: 924 924
Size: 3908
AclI AA'CG_TT
Cuts at: 3748 3748
Size: 3908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1733 2165 2912 3180 1733
Size: 432 747 268 2461
Fragments arranged by size:
2461 747 432 268
AflIII A'CryG_T
Cuts at: 1260 2364 3341 1260
Size: 1104 977 1827
Fragments arranged by size:
1827 1104 977
AgeI A'CCGG_T
Cuts at: 2296 2296
Size: 3908
AlwNI CAG_nnn'CTG
Cuts at: 1213 2780 1213
Size: 1567 2341
ApaI G_GGCC'C
Cuts at: 187 346 389 428 463 187
Size: 159 43 39 35 3632
Fragments arranged by size:
3632 159 43 39 35
ApaLI G'TGCA_C
Cuts at: 2678 2678
Size: 3908
ApoI r'AATT_y
Cuts at: 912 3261 3517 912
Size: 2349 256 1303
Fragments arranged by size:
2349 1303 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3908
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3908
AvaI C'yCGr_G
Cuts at: 339 432 454 462 928 339
Size: 93 22 8 466 3319
Fragments arranged by size:
3319 466 93 22 8
BamHI G'GATC_C
Cuts at: 933 933
Size: 3908
BanI G'GyrC_C
Cuts at: 219 294 408 414 924 1338 1586 1621 219
Size: 75 114 6 510 414 248 35 2506
Fragments arranged by size:
2506 510 414 248 114 75 35 6
BanII G_rGCy'C
Cuts at: 187 346 389 428 463 686 922 1952 187
Size: 159 43 39 35 223 236 1030 2143
Fragments arranged by size:
2143 1030 236 223 159 43 39 35
BbeI G_GCGC'C
Cuts at: 223 298 418 1342 1590 223
Size: 75 120 924 248 2541
Fragments arranged by size:
2541 924 248 120 75
BciVI GTATCCnnnnn_n'
Cuts at: 1806 2567 3908 1806
Size: 761 1341 1806
Fragments arranged by size:
1806 1341 761
BclI T'GATC_A
Cuts at: 1428 1428
Size: 3908
BglI GCCn_nnn'nGGC
Cuts at: 292 423 292
Size: 131 3777
BglII A'GATC_T
Cuts at: 704 1423 3460 704
Size: 719 2037 1152
Fragments arranged by size:
2037 1152 719
BlpI GC'TnA_GC
Cuts at: 1020 1020
Size: 3908
Bme1580I G_kGCm'C
Cuts at: 187 346 389 428 463 469 1533 1626 2682
Size: 159 43 39 35 6 1064 93 1056
Cuts at: 2682 187
Size: 1413
Fragments arranged by size:
1413 1064 1056 159 93 43 39 35
6
BmrI ACTGGGnnnn_n'
Cuts at: 330 1120 1201 1536 3680 330
Size: 790 81 335 2144 558
Fragments arranged by size:
2144 790 558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2275 2275
Size: 3908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 377 628 634 228
Size: 149 251 6 3502
Fragments arranged by size:
3502 251 149 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1084 1963 2455 2753 1084
Size: 879 492 298 2239
Fragments arranged by size:
2239 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3612 3612
Size: 3908
BsaAI yAC'GTr
Cuts at: 258 1261 258
Size: 1003 2905
BsaBI GATnn'nnATC
Cuts at: 6 1422 3459 6
Size: 1416 2037 455
Fragments arranged by size:
2037 1416 455
BsaHI Gr'CG_yC
Cuts at: 220 295 415 499 1339 1587 2289 3785 220
Size: 75 120 84 840 248 702 1496 343
Fragments arranged by size:
1496 840 702 343 248 120 84 75
BsaWI w'CCGG_w
Cuts at: 1097 1618 2296 2570 2717 3128 1097
Size: 521 678 274 147 411 1877
Fragments arranged by size:
1877 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 607 637 855 885 607
Size: 30 218 30 3630
Fragments arranged by size:
3630 218 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 858 904 858
Size: 46 3862
BseYI C'CCAG_C
Cuts at: 156 366 810 841 1333 2091 2668 3164 156
Size: 210 444 31 492 758 577 496 900
Fragments arranged by size:
900 758 577 496 492 444 210 31
BsiEI CG_ry'CG
Cuts at: 104 533 1450 1496 2704 104
Size: 429 917 46 1208 1308
Fragments arranged by size:
1308 1208 917 429 46
BsiHKAI G_wGCw'C
Cuts at: 922 1700 1890 2682 922
Size: 778 190 792 2148
Fragments arranged by size:
2148 792 778 190
BsmI GAATG_Cn'
Cuts at: 379 379
Size: 3908
Bsp1286I G_dGCh'C
Cuts at: 187 346 389 428 463 469 686 922 1533
Size: 159 43 39 35 6 217 236 611
Cuts at: 1533 1626 1700 1890 1952 2682 187
Size: 93 74 190 62 730 1413
Fragments arranged by size:
1413 730 611 236 217 190 159 93
74 62 43 39 35 6
BspEI T'CCGG_A
Cuts at: 3128 3128
Size: 3908
BspHI T'CATG_A
Cuts at: 3084 3084
Size: 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 687 958 1172 1474 1855 687
Size: 271 214 302 381 2740
Fragments arranged by size:
2740 381 302 271 214
BsrBI CCG'CTC
Cuts at: 50 2200 2254 3738 3903 50
Size: 2150 54 1484 165 55
Fragments arranged by size:
2150 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1820 1820
Size: 3908
BsrFI r'CCGG_y
Cuts at: 264 411 719 1906 2087 2296 264
Size: 147 308 1187 181 209 1876
Fragments arranged by size:
1876 1187 308 209 181 147
BssSI C'ACGA_G
Cuts at: 2179 2537 2179
Size: 358 3550
BstAPI GCAn_nnn'nTGC
Cuts at: 1285 1285
Size: 3908
BstBI TT'CG_AA
Cuts at: 2269 2269
Size: 3908
BstXI CCAn_nnnn'nTGG
Cuts at: 114 858 114
Size: 744 3164
BstYI r'GATC_y
Cuts at: 1 704 933 1400 1423 1758 2004 3016 3102
Size: 703 229 467 23 335 246 1012 86
Cuts at: 3102 3114 3460 1
Size: 12 346 449
Fragments arranged by size:
1012 703 467 449 346 335 246 229
86 23 12
BtgI C'CryG_G
Cuts at: 347 347
Size: 3908
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1858 2039 2202 1858
Size: 181 163 3564
Fragments arranged by size:
3564 181 163
BtsI GCAGTG_nn'
Cuts at: 1151 1151
Size: 3908
DraI TTT'AAA
Cuts at: 907 907
Size: 3908
DrdI GACnn_nn'nnGTC
Cuts at: 1614 2472 1614
Size: 858 3050
EaeI y'GGCC_r
Cuts at: 530 803 860 1493 1667 2058 2085 3430 530
Size: 273 57 633 174 391 27 1345 1008
Fragments arranged by size:
1345 1008 633 391 273 174 57 27
EagI C'GGCC_G
Cuts at: 530 1493 530
Size: 963 2945
EarI CTCTTCn'nnn_
Cuts at: 1931 2141 1931
Size: 210 3698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 510 669 2116 2426 2572 3718 510
Size: 159 1447 310 146 1146 700
Fragments arranged by size:
1447 1146 700 310 159 146
EcoICRI GAG'CTC
Cuts at: 920 920
Size: 3908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 377 628 634 1733 2165 2912 3180 228
Size: 149 251 6 1099 432 747 268 956
Fragments arranged by size:
1099 956 747 432 268 251 149 6
EcoO109I rG'GnC_Cy
Cuts at: 343 386 459 831 1047 343
Size: 43 73 372 216 3204
Fragments arranged by size:
3204 372 216 73 43
EcoRI G'AATT_C
Cuts at: 912 912
Size: 3908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1927 1927
Size: 3908
FspI TGC'GCA
Cuts at: 1689 1689
Size: 3908
HaeII r_GCGC'y
Cuts at: 223 298 418 1342 1590 2612 3591 223
Size: 75 120 924 248 1022 979 540
Fragments arranged by size:
1022 979 924 540 248 120 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1750 1780 1782 1812 1750
Size: 30 2 30 3846
Fragments arranged by size:
3846 30 30 2
HincII GTy'rAC
Cuts at: 947 947
Size: 3908
KasI G'GCGC_C
Cuts at: 219 294 414 1338 1586 219
Size: 75 120 924 248 2541
Fragments arranged by size:
2541 924 248 120 75
KpnI G_GTAC'C
Cuts at: 928 928
Size: 3908
MluI A'CGCG_T
Cuts at: 3341 3341
Size: 3908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2579 2763 2579
Size: 184 3724
MscI TGG'CCA
Cuts at: 862 1669 862
Size: 807 3101
MslI CAynn'nnrTG
Cuts at: 112 1897 2024 112
Size: 1785 127 1996
Fragments arranged by size:
1996 1785 127
MspA1I CmG'CkG
Cuts at: 349 551 1019 1333 1693 2706 2951 349
Size: 202 468 314 360 1013 245 1306
Fragments arranged by size:
1306 1013 468 360 314 245 202
NaeI GCC'GGC
Cuts at: 266 413 2089 266
Size: 147 1676 2085
Fragments arranged by size:
2085 1676 147
NarI GG'CG_CC
Cuts at: 220 295 415 1339 1587 220
Size: 75 120 924 248 2541
Fragments arranged by size:
2541 924 248 120 75
NgoMIV G'CCGG_C
Cuts at: 264 411 2087 264
Size: 147 1676 2085
Fragments arranged by size:
2085 1676 147
NspI r_CATG'y
Cuts at: 961 1461 2368 961
Size: 500 907 2501
Fragments arranged by size:
2501 907 500
PasI CC'CwG_GG
Cuts at: 421 825 421
Size: 404 3504
PciI A'CATG_T
Cuts at: 2364 2364
Size: 3908
PflMI CCAn_nnn'nTGG
Cuts at: 606 606
Size: 3908
PmeI GTTT'AAAC
Cuts at: 907 907
Size: 3908
PmlI CAC'GTG
Cuts at: 258 258
Size: 3908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3047 3079 3047
Size: 32 3876
PspOMI G'GGCC_C
Cuts at: 183 342 385 424 459 183
Size: 159 43 39 35 3632
Fragments arranged by size:
3632 159 43 39 35
PstI C_TGCA'G
Cuts at: 955 1640 955
Size: 685 3223
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3908
PvuII CAG'CTG
Cuts at: 1333 1693 1333
Size: 360 3548
RsrII CG'GwC_CG
Cuts at: 2103 2103
Size: 3908
SacI G_AGCT'C
Cuts at: 922 922
Size: 3908
SacII CC_GC'GG
Cuts at: 350 350
Size: 3908
SalI G'TCGA_C
Cuts at: 945 945
Size: 3908
SapI GCTCTTCn'nnn_
Cuts at: 1931 2141 1931
Size: 210 3698
SbfI CC_TGCA'GG
Cuts at: 955 955
Size: 3908
ScaI AGT'ACT
Cuts at: 3425 3425
Size: 3908
SfcI C'TryA_G
Cuts at: 33 951 1636 2629 2820 33
Size: 918 685 993 191 1121
Fragments arranged by size:
1121 993 918 685 191
SfoI GGC'GCC
Cuts at: 221 296 416 1340 1588 221
Size: 75 120 924 248 2541
Fragments arranged by size:
2541 924 248 120 75
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3908
SmaI CCC'GGG
Cuts at: 341 434 464 930 341
Size: 93 30 466 3319
Fragments arranged by size:
3319 466 93 30
SmlI C'TyrA_G
Cuts at: 1063 1978 2470 2732 1063
Size: 915 492 262 2239
Fragments arranged by size:
2239 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3908
SphI G_CATG'C
Cuts at: 961 1461 961
Size: 500 3408
SrfI GCCC'GGGC
Cuts at: 341 434 464 341
Size: 93 30 3785
Fragments arranged by size:
3785 93 30
StyI C'CwwG_G
Cuts at: 311 566 1042 1396 311
Size: 255 476 354 2823
Fragments arranged by size:
2823 476 354 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1491 1624 2083 2292 1491
Size: 133 459 209 3107
Fragments arranged by size:
3107 459 209 133
TatI w'GTAC_w
Cuts at: 3423 3423
Size: 3908
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 855 887 3425 3457 855
Size: 32 2538 32 1306
Fragments arranged by size:
2538 1306 32 32
Tth111I GACn'n_nGTC
Cuts at: 1705 1705
Size: 3908
XbaI T'CTAG_A
Cuts at: 939 939
Size: 3908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 177 1403 177
Size: 1226 2682
XmaI C'CCGG_G
Cuts at: 339 432 462 928 339
Size: 93 30 466 3319
Fragments arranged by size:
3319 466 93 30
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PmeI PmlI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PfoI PpuMI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SspI StuI SwaI
XhoI XmnI ZraI