Restriction map for
pF1KB9697
(Circular) (Six-base) MAPSORT of: pF1KB9697.seq Check: 7588 from: 1 to: 3923
pF1KB9697 3923 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 961 961
Size: 3923
Acc65I G'GTAC_C
Cuts at: 939 939
Size: 3923
AclI AA'CG_TT
Cuts at: 3763 3763
Size: 3923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1748 2180 2927 3195 1748
Size: 432 747 268 2476
Fragments arranged by size:
2476 747 432 268
AflIII A'CryG_T
Cuts at: 1275 2379 3356 1275
Size: 1104 977 1842
Fragments arranged by size:
1842 1104 977
AgeI A'CCGG_T
Cuts at: 2311 2311
Size: 3923
AlwNI CAG_nnn'CTG
Cuts at: 633 674 1228 2795 633
Size: 41 554 1567 1761
Fragments arranged by size:
1761 1567 554 41
ApaLI G'TGCA_C
Cuts at: 280 2693 280
Size: 2413 1510
ApoI r'AATT_y
Cuts at: 927 3276 3532 927
Size: 2349 256 1318
Fragments arranged by size:
2349 1318 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
AvaI C'yCGr_G
Cuts at: 571 943 571
Size: 372 3551
BamHI G'GATC_C
Cuts at: 948 948
Size: 3923
BanI G'GyrC_C
Cuts at: 168 408 697 902 939 1353 1601 1636 168
Size: 240 289 205 37 414 248 35 2455
Fragments arranged by size:
2455 414 289 248 240 205 37 35
BanII G_rGCy'C
Cuts at: 326 529 572 584 937 1967 326
Size: 203 43 12 353 1030 2282
Fragments arranged by size:
2282 1030 353 203 43 12
BbeI G_GCGC'C
Cuts at: 906 1357 1605 906
Size: 451 248 3224
Fragments arranged by size:
3224 451 248
BbsI GAAGACnn'nnnn_
Cuts at: 167 167
Size: 3923
BciVI GTATCCnnnnn_n'
Cuts at: 1821 2582 3923 1821
Size: 761 1341 1821
Fragments arranged by size:
1821 1341 761
BclI T'GATC_A
Cuts at: 1443 1443
Size: 3923
BglI GCCn_nnn'nGGC
Cuts at: 887 887
Size: 3923
BglII A'GATC_T
Cuts at: 1438 3475 1438
Size: 2037 1886
BlpI GC'TnA_GC
Cuts at: 1035 1035
Size: 3923
Bme1580I G_kGCm'C
Cuts at: 173 284 700 1548 1641 2697 173
Size: 111 416 848 93 1056 1399
Fragments arranged by size:
1399 1056 848 416 111 93
BmgBI CAC'GTC
Cuts at: 390 390
Size: 3923
BmrI ACTGGGnnnn_n'
Cuts at: 267 416 1135 1216 1551 3695 267
Size: 149 719 81 335 2144 495
Fragments arranged by size:
2144 719 495 335 149 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 3923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 397 448 556 586 310
Size: 87 51 108 30 3647
Fragments arranged by size:
3647 108 87 51 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1099 1978 2470 2768 1099
Size: 879 492 298 2254
Fragments arranged by size:
2254 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 444 3627 444
Size: 3183 740
BsaAI yAC'GTr
Cuts at: 279 1276 279
Size: 997 2926
BsaBI GATnn'nnATC
Cuts at: 6 1437 3474 6
Size: 1431 2037 455
Fragments arranged by size:
2037 1431 455
BsaHI Gr'CG_yC
Cuts at: 903 1354 1602 2304 3800 903
Size: 451 248 702 1496 1026
Fragments arranged by size:
1496 1026 702 451 248
BsaWI w'CCGG_w
Cuts at: 1112 1633 2311 2585 2732 3143 1112
Size: 521 678 274 147 411 1892
Fragments arranged by size:
1892 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 424 454 424
Size: 30 3893
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 520 523 520
Size: 3 3920
BseYI C'CCAG_C
Cuts at: 483 488 493 743 876 1348 2106 2683 3179
Size: 5 5 250 133 472 758 577 496
Cuts at: 3179 483
Size: 1227
Fragments arranged by size:
1227 758 577 496 472 250 133 5
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 355
Size: 3923
BsiEI CG_ry'CG
Cuts at: 104 1465 1511 2719 104
Size: 1361 46 1208 1308
Fragments arranged by size:
1361 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 284 326 937 1715 1905 2697 284
Size: 42 611 778 190 792 1510
Fragments arranged by size:
1510 792 778 611 190 42
Bsp1286I G_dGCh'C
Cuts at: 173 284 326 529 572 584 700 937 1548
Size: 111 42 203 43 12 116 237 611
Cuts at: 1548 1641 1715 1905 1967 2697 173
Size: 93 74 190 62 730 1399
Fragments arranged by size:
1399 730 611 237 203 190 116 111
93 74 62 43 42 12
BspEI T'CCGG_A
Cuts at: 3143 3143
Size: 3923
BspHI T'CATG_A
Cuts at: 3099 3099
Size: 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 455 650 973 1187 1489 1870 455
Size: 195 323 214 302 381 2508
Fragments arranged by size:
2508 381 323 302 214 195
BsrBI CCG'CTC
Cuts at: 50 519 900 2215 2269 3753 3918 50
Size: 469 381 1315 54 1484 165 55
Fragments arranged by size:
1484 1315 469 381 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1835 1835
Size: 3923
BsrFI r'CCGG_y
Cuts at: 1921 2102 2311 1921
Size: 181 209 3533
Fragments arranged by size:
3533 209 181
BssHII G'CGCG_C
Cuts at: 793 795 797 793
Size: 2 2 3919
Fragments arranged by size:
3919 2 2
BssSI C'ACGA_G
Cuts at: 205 2194 2552 205
Size: 1989 358 1576
Fragments arranged by size:
1989 1576 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1300 1300
Size: 3923
BstBI TT'CG_AA
Cuts at: 2284 2284
Size: 3923
BstYI r'GATC_y
Cuts at: 1 183 614 948 1415 1438 1773 2019 3031
Size: 182 431 334 467 23 335 246 1012
Cuts at: 3031 3117 3129 3475 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 431 346 335 334 246
182 86 23 12
BtgI C'CryG_G
Cuts at: 404 882 404
Size: 478 3445
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1873 2054 2217 1873
Size: 181 163 3579
Fragments arranged by size:
3579 181 163
BtsI GCAGTG_nn'
Cuts at: 234 1166 234
Size: 932 2991
DraI TTT'AAA
Cuts at: 922 922
Size: 3923
DraIII CAC_nnn'GTG
Cuts at: 279 279
Size: 3923
DrdI GACnn_nn'nnGTC
Cuts at: 1629 2487 1629
Size: 858 3065
EaeI y'GGCC_r
Cuts at: 429 893 1508 1682 2073 2100 3445 429
Size: 464 615 174 391 27 1345 907
Fragments arranged by size:
1345 907 615 464 391 174 27
EagI C'GGCC_G
Cuts at: 1508 1508
Size: 3923
EarI CTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 571 607 2131 2441 2587 3733 571
Size: 36 1524 310 146 1146 761
Fragments arranged by size:
1524 1146 761 310 146 36
EcoICRI GAG'CTC
Cuts at: 324 935 324
Size: 611 3312
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 397 448 556 586 1748 2180 2927 3195
Size: 87 51 108 30 1162 432 747 268
Cuts at: 3195 310
Size: 1038
Fragments arranged by size:
1162 1038 747 432 268 108 87 51
30
EcoO109I rG'GnC_Cy
Cuts at: 888 1062 888
Size: 174 3749
EcoRI G'AATT_C
Cuts at: 927 927
Size: 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 717 1942 717
Size: 1225 2698
FspI TGC'GCA
Cuts at: 805 1704 805
Size: 899 3024
HaeII r_GCGC'y
Cuts at: 733 906 1357 1605 2627 3606 733
Size: 173 451 248 1022 979 1050
Fragments arranged by size:
1050 1022 979 451 248 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1765 1795 1797 1827 1765
Size: 30 2 30 3861
Fragments arranged by size:
3861 30 30 2
HincII GTy'rAC
Cuts at: 962 962
Size: 3923
KasI G'GCGC_C
Cuts at: 902 1353 1601 902
Size: 451 248 3224
Fragments arranged by size:
3224 451 248
KpnI G_GTAC'C
Cuts at: 943 943
Size: 3923
MluI A'CGCG_T
Cuts at: 3356 3356
Size: 3923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 617 2594 2778 617
Size: 1977 184 1762
Fragments arranged by size:
1977 1762 184
MscI TGG'CCA
Cuts at: 431 1684 431
Size: 1253 2670
MslI CAynn'nnrTG
Cuts at: 1912 2039 1912
Size: 127 3796
MspA1I CmG'CkG
Cuts at: 348 653 743 866 884 1034 1348 1708 2721
Size: 305 90 123 18 150 314 360 1013
Cuts at: 2721 2966 348
Size: 245 1305
Fragments arranged by size:
1305 1013 360 314 305 245 150 123
90 18
NaeI GCC'GGC
Cuts at: 2104 2104
Size: 3923
NarI GG'CG_CC
Cuts at: 903 1354 1602 903
Size: 451 248 3224
Fragments arranged by size:
3224 451 248
NcoI C'CATG_G
Cuts at: 404 404
Size: 3923
NgoMIV G'CCGG_C
Cuts at: 2102 2102
Size: 3923
NspI r_CATG'y
Cuts at: 976 1476 2383 976
Size: 500 907 2516
Fragments arranged by size:
2516 907 500
PciI A'CATG_T
Cuts at: 2379 2379
Size: 3923
PflMI CCAn_nnn'nTGG
Cuts at: 789 789
Size: 3923
PmeI GTTT'AAAC
Cuts at: 922 922
Size: 3923
PmlI CAC'GTG
Cuts at: 279 279
Size: 3923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 3891
PstI C_TGCA'G
Cuts at: 353 970 1655 353
Size: 617 685 2621
Fragments arranged by size:
2621 685 617
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3923
PvuII CAG'CTG
Cuts at: 348 1348 1708 348
Size: 1000 360 2563
Fragments arranged by size:
2563 1000 360
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 3923
SacI G_AGCT'C
Cuts at: 326 937 326
Size: 611 3312
SacII CC_GC'GG
Cuts at: 885 885
Size: 3923
SalI G'TCGA_C
Cuts at: 960 960
Size: 3923
SapI GCTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
SbfI CC_TGCA'GG
Cuts at: 970 970
Size: 3923
ScaI AGT'ACT
Cuts at: 3440 3440
Size: 3923
SexAI A'CCwGG_T
Cuts at: 164 434 164
Size: 270 3653
SfcI C'TryA_G
Cuts at: 33 349 966 1651 2644 2835 33
Size: 316 617 685 993 191 1121
Fragments arranged by size:
1121 993 685 617 316 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 887 887
Size: 3923
SfoI GGC'GCC
Cuts at: 904 1355 1603 904
Size: 451 248 3224
Fragments arranged by size:
3224 451 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
SmaI CCC'GGG
Cuts at: 573 945 573
Size: 372 3551
SmlI C'TyrA_G
Cuts at: 1078 1993 2485 2747 1078
Size: 915 492 262 2254
Fragments arranged by size:
2254 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3923
SphI G_CATG'C
Cuts at: 976 1476 976
Size: 500 3423
SrfI GCCC'GGGC
Cuts at: 573 573
Size: 3923
StyI C'CwwG_G
Cuts at: 404 1057 1411 404
Size: 653 354 2916
Fragments arranged by size:
2916 653 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 669 1506 1639 2098 2307 669
Size: 837 133 459 209 2285
Fragments arranged by size:
2285 837 459 209 133
TatI w'GTAC_w
Cuts at: 3438 3438
Size: 3923
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 424 456 3440 3472 424
Size: 32 2984 32 875
Fragments arranged by size:
2984 875 32 32
Tth111I GACn'n_nGTC
Cuts at: 1720 1720
Size: 3923
XbaI T'CTAG_A
Cuts at: 954 954
Size: 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 424 1418 424
Size: 994 2929
XmaI C'CCGG_G
Cuts at: 571 943 571
Size: 372 3551
XmnI GAAnn'nnTTC
Cuts at: 134 313 134
Size: 179 3744
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SgrAI SnaBI SspI StuI SwaI XhoI ZraI